| 赵艳，阮云泽，张晓波.王草砖红壤土壤根际解磷菌的分离鉴定及培养条件研究[J].广东农业科学,2014,41(19):35-38 |
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王草砖红壤土壤根际解磷菌的分离鉴定及培养条件研究 |
| Study on isolation, identification and culture condition of rhizosphere phosphate-solubilizing microorganisms from red paddy soil of king grass |
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| DOI： |
| 中文关键词: 王草 解磷菌 分离 鉴定 |
| 英文关键词: Cynodon dactylon L. phosphate-solubilizing microorganism isolation identification |
| 基金项目:海南省自然科学基金（312060);海南大学青年基金项目(qnjj1201) |
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| 摘要点击次数: 1135 |
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| 中文摘要: |
| 从王草（Cynodon dactylon L.）根际砖红壤土壤中筛选到21株解磷菌，对其形态学和生理生化特性进行了测定，最后将筛选出的解磷能力较强且无拮抗反应的4 株解磷细菌组合成为解磷菌群，对其最适温度、最适pH值和最适生长时间进行了研究。结果表明，菌株2-5、2-6、2、8-1、2-9、3-8、3-8-1、4-1-2、4-2、4-5 为巨大芽孢杆菌（Bacillus megaterium）；菌株2-7-1、2-8、3-6-1、4-1-1、4-3为伯克霍尔德菌属（Burkholderia sp）；菌株4-4、2-7、2-8-2、3-6、4-3-1为蜡样芽孢杆菌（Bacillus cereus）。解磷菌群的最适生长温度为25~30℃，最适生长pH 值为6.5~7.0。最适生长时间为90h。 |
| 英文摘要: |
| Twenty-one strains of Phosphate-solubilizing bacteria were screened from red paddy soil samples of king grass, and physiological and biochemical characters of these strains were determined. Finally, 4 phosphate-solubilizing bacterial strains with stronger phosphate-solubilizing ability but without antagonism were screened, and combined as flora
of phosphate-solubilizing bacteria, then their optimum temperature, optimum initial pH and optimum growth time were
studied. The results showed that Strains 2-5, 2-6, 2-8-1, 2-9, 3-8, 3-8-1, 4-1-2, 4-2, 4-5 were Bacillus megaterium,
Strains 2-7-1, 2-8, 3-6-1, 4-1-1, 4-3 were Burkholderia sp., Strains 4-4, 2-7, 2-8-2, 3-6, 4-3-1 were Bacillus cereus.
The optimum temperature for phosphate-solubilizing bacteria flora was 25℃-30℃, the optimum initial pH was 6.5-7.0 and
the optimum growth time was 90 h. |
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