| 王轶男，陈雪，盖颖.毛白杨木质素合成酶基因F5H克隆与生物信息学分析[J].广东农业科学,2014,41(20):131-135 |
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毛白杨木质素合成酶基因F5H克隆与生物信息学分析 |
| Cloning and bioinformatics analysis on Lignin synthetase gene Ferulate-5-hydroxylase of Populus tomentosa |
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| DOI： |
| 中文关键词: 毛白杨 阿魏酸-5-羟化酶 基因克隆 生物信息学 |
| 英文关键词: Populos tomentosa Ferulate-5-hydroxylase cloning bioinformatics |
| 基金项目:\ |
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| 摘要点击次数: 1249 |
| 全文下载次数: 490 |
| 中文摘要: |
| 克隆毛白杨阿魏酸-5-羟基化酶（F5H）基因全长序列，并通过生物信息学分析，为进一步研究F5H 基因功能提供基础。利用RT-PCR方法成功从毛白杨741组培苗叶片中克隆了F5H 基因，并对F5H 基因的核苷酸序列及推导的氨基酸序列进行分析，包括疏水性-亲水性、氨基酸翻译后修饰、亚细胞定位、跨膜结构域、蛋白质二级三级功能结构域等进行分析预测和推断。获得的基因长度为1 686 bp，编码区1542 bp，与已公布的毛果杨F5H mRNA序列（GenBank 登录号为AJ010324)的编码区相似性达97.45%,与其他植物的该基因序列具有同源性。F5H 是亲水性较强的蛋白，整条肽链有1 个跨膜结构域；通过亚细胞定位表明F5H 主要位于质体中，并含有磷酸化位点有19 个。F5H 含有丰富的二级结构，卷曲占44.44%、折叠占7.02%、螺旋占48.54%，并预测了其三维结构。分析结果显示，植物的阿魏酸-5-羟基化酶是一个具有跨膜结构域的亲水性蛋白，在内质网等分泌途径中表达。 |
| 英文摘要: |
| It provides a basis for further study of the gene function of Ferulate-5-hydroxylase(F5H) to clone and make
bioinformatics analysis of F5H gene in Populus tomentosa. F5H gene was cloned from P. tomentosa leaves by RT-PCR
method. The physical chemistry characteristics including hydrophobsity, post -translational modification, subcellular
localization, transmembrane peptide, secondary and 3D structure of F5H were analyzed by applications on-line. The length
of acquired sequence was 1 686 bp and the sequence in coding region is 1542bp. Compared with the coding region of F5H
mRNA in P. trichocarpa (Accession Number: AJ010324), the resemblance was 97.45% . The results of subcellular
localization showed that the F5H mainly lies in plasmid, which was analyzed by prediction tools. The secondary structure of F5H was various: 44.44% of Alpha helix, 7.02% of Extended strand and 48.54% of Random coil. 3D structure of F5H was
predicted by SWISS-MODE. The results show that F5H is a hydrophilic protein with two transmembrane peptides and
expresses in the endoplasmic reticulum and other secretory pathways. |
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