| 王凯强，朱丹，逄秀梅，米晓霞，李艳华，尹昭坤，李强强，陈刚.基于脂肪酸差异的有机猪肉溯源识别研究[J].广东农业科学,2014,41(21):106-110 |
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基于脂肪酸差异的有机猪肉溯源识别研究 |
| Research on traceability identification for organic pork based on fatty acid differences |
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| DOI： |
| 中文关键词: 溯源 有机猪肉 普通猪肉 脂肪酸 |
| 英文关键词: traceability organic pork conventional pork fatty acid |
| 基金项目:农业部引进国际先进农业科学技术计划（948）项目（2013-S1）；国际原子能机构（IAEA）CRP项目（16567） |
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| 中文摘要: |
| 有机猪肉的真假鉴别是优质农产品质量安全监管的难点。利用脂肪酸作为表征因子，对有机猪肉的真实性进行溯源识别的方法进行研究。利用气相色谱-氢火焰离子化检测器法测定了有机和普通猪肉中21种脂肪。样品用氯乙酰-甲醇甲酯化，正己烷提取，CP7420毛细管柱（100m*0.25mm,0.25μm）分离，高纯氮作载气；单因素方差分析结果表明，21种脂肪酸含量中，C12:0、C15:0、C16:0、C16:1、C18:2n6t、C18:2n6c、C18:3(n3)、C20:1、C21:0、C20:3(n6)、C22:0、C20:5(n3)、C24:0、C24:1、C22:6(n3)有显著差异；利用SPSS 软件分析原始数据，建立了有机猪肉溯源识别模型，作判别分析和交叉验证，有机猪肉的正确判别率达到了100%；同时利用Origin软件作有机和普通猪肉PCA图。
结果表明有机猪肉能够有效溯源识别。 |
| 英文摘要: |
| One problem of quality and safety supervision for high-grade agro-products lay in identity authentication
for organic pork. The test used fatty acid as characterization factor to study the traceability identification of organic pork facticity. 21 fatty acids of organic and conventional pork were determined by gas chromatography-FID. After methylesterification of chloroacetic methanol, dissolved in n-hexane, fatty acids were separated by a capillary column of CP7420 (100m*0.25mm,0.25μm) and high purity nitride was served as carrier gas. Results analyzed with one-way Anova
showed that fatty acids existed significant difference on content in organic and conventional pork, namely C12:0, C15:0,
C16:0, C16:1, C18:2n6t, C18:2n6c, C18:3(n3), C20:1, C21:0, C20:3(n6), C22:0, C20:5(n3), C24:0, C24:1, C22:6(n3). Initial
data analyses and origin recognition model of organic pork was established, by discriminant analysis and cross-validation
with SPSS 22.0, the correct rate of the discriminant analysis was up to 100%. Meanwhile principal component analysis
figure of organic and conventional pork was constructed by Origin. The result indicated that organic pork could be traced
validly |
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