| 郭培国，许兰桂，何嘉仪，夏岩石，黄红弟，郑岩松，张华，李荣华.菜心种质材料的分子身份证构建研究[J].广东农业科学,2014,41(23):116-122 |
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菜心种质材料的分子身份证构建研究 |
| Establishment of molecular identity in flowering Chinese cabbage germplasm |
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| DOI： |
| 中文关键词: 菜心 种质 MFLP 分子身份证 |
| 英文关键词: flowering Chinese cabbage germplasm MFLP molecular identity |
| 基金项目:广州市科技计划项目（1212011541，2014J4100123）；广东省教育厅科技创新项目（2012KJCX0083）；广州市教育局科研项目（2015-2016） |
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| 中文摘要: |
| 建立菜心种质材料的分子身份证有利于菜心种质材料的收集、保存、管理和利用。利用荧光MFLP 标记技术分析供试的36 份菜心种质材料，从200 对选择性扩增引物组合中筛选出扩增产物清晰稳定的18 对引物组合。这些引物组合在36 份菜心种质材料中有3~31 等位变异，多态性信息量（PIC）和标记指数值（MI）分别在0.26~0.98和0.8~30.4 之间。主坐标分析表明，18 对引物组合可以区分各供试材料，并按遗传相似性将供试材料归为3 个类群。考虑到使用最少引物组合区分不同种质材料，筛选和确定出PIC 和MI 值最高的3 个MFLP 选择性扩增引物组合（tfCCTA(CA)7/mAAG、tfGGTC(ATT)5/mCAA 和tfCCTA(CA)7/mCTC）作为核心引物组合，建立3 个引物组合检测到每份种质材料等位变异有（1）无（0）的二进制数据，并将其转换成简捷直观的16 进制数据，从而构建了供试菜心种质材料的分子身份证。 |
| 英文摘要: |
| Eestablishment of molecular identity is beneficial to effectively serve the preservation and utilization of the
germplasm in flowering Chinese cabbage. In this study, the fluorescent microsatellite -anchored fragment length
polymorphism (MFLP) technique was utilized to genotype 36 accessions of the flowering Chinese cabbage germplasm
collected from different parts of China, and 18 appropriate primer pairs amplified stably and unambiguously polymorphic
bands were chosen from 200 selective amplification primer combinations to detect the polymorphic alleles among the
accessions. The results showed that the polymorphic alleles for 18 primer pairs ranged from 3 to 31 with the average of
12.5 per primer combination. Polymorphism information content (PIC) and marker index (MI) ranged in 0.26-0.98 and 0.8-
30.4, respectively. The principal coordinate analysis (PCA) showed that 36 accessions could be clearly distinguished by 18
primer combinations, which divided into 3 groups based on genetic similarity. According to the principal of distinguishing
accessions with the less primer pairs, three primer pairs including tfCCTA(CA)7/mAAG, tfGGTC(ATT)5/mCAA and tfCCTA
(CA)7/mCTC with the highest values of PIC and MI were selected as the core primer combinations. Based on the binary
data of the allele variants (allele present is 1, absent is 0) of the accessions, the molecular identity for each accession of flowering Chinese cabbage was established by converting the corresponding binary data into the simple and intuitive hexadecimal data. |
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