| 商龙臣1，李琴1，张驰1,2.正交试验优化红豆杉硒多糖的提取[J].广东农业科学,2014,41(24):95-98 |
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正交试验优化红豆杉硒多糖的提取 |
| Extraction of Se-polysaccharides from Taxuschinensis by orthogonal test |
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| DOI： |
| 中文关键词: 红豆杉 硒多糖 提取分离 正交优化 |
| 英文关键词: Taxus chinensis Se-polysaccharide extraction and separation orthogonal optimization |
| 基金项目:国家自然科学基金（61263030/F030304）；湖北省
科技厅重点基金（011CDA011）；生物资源保护与利用湖北省重
点实验室开放基金（PKLHB1113）；湖北省大学科技创新项目
（01310517016）; 2013 年湖北省战略性新兴（支柱）产业人才培
养（生物工程）项目 |
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| 中文摘要: |
| 以恩施高硒地区的珍贵药用植物红豆杉为研究对象，以料液比、水浴浸提时间以及微波处理时间为试
验因素设计正交试验，优化了红豆杉硒多糖的提取分离纯化工艺。通过硫酸苯酚法测定红豆杉中硒多糖的含量，采
用原子荧光法测定硒多糖中硒的含量，并以SPSS 统计软件对正交试验结果进行处理和分析。结果表明，3 因素对硒
多糖提取率影响大小为料液比>微波处理时间>水提时间，且红豆杉中硒多糖的最佳提取条件为料液比1颐80（W/V）、
水浴浸提时间30 min、微波处理时间5 min。在此条件下，测得红豆杉中硒多糖含量为1.96%，硒多糖中硒含量为
0.0555 滋g/g。 |
| 英文摘要: |
| In this thesis, Taxus chinensis, a kind of precious medicinal plant that grows in Enshi where it is a high
selenium areas in China, was used as experimental material. Solid -liquid ratio, water extraction time and microwave
processing time were the three experimental factors, then orthogonal experiment was designed to find a more efficient way
to extract and separate Se-polysaccharide from T. chinensis. Based on that, the phenol sulfuric acid method was used to
detect the content of Se-polysaccharide in T. chinensis, and the atomic fluorescence method was used to detect the content
of Se in Se -polysaccharide. In addition, statistical software, SPSS, was used to analyze and process the orthogonal
experiment results. The results showed that the best conditions to extract this kind of polysaccharide were as follows: the
solid-liquid ratio was 180 (W/V), water extraction time was 130 min, microwave processing time was 5 min. Among these
three factors, the influence order on polysaccharide extraction rate was solid-liquid ratio>microwave processing time>water
extraction time. Under these conditions, the content of Se-polysaccharide was up to 1.96%, selenium content in Sepolysaccharide
was 0.0555 g/g. |
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