| 柳忠玉1，赵树进2.转PcRS基因拟南芥植株表型分析[J].广东农业科学,2014,41(24):134-137 |
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转PcRS基因拟南芥植株表型分析 |
| Phenotypic analysis of the transgenicPcRS Arabidopsis plants |
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| DOI： |
| 中文关键词: 虎杖 白藜芦醇合酶 转基因拟南芥 花青素 种皮 |
| 英文关键词: Polygonum cuspidatum resveratrol synthase transgenic Arabidopsis anthocyanin seed coat |
| 基金项目:长江大学博士启动基金（801100010122）；广东省
自然科学基金（10151001002000012） |
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| 中文摘要: |
| 为了研究虎杖（Polygonum cuspidatum Sieb. et Zucc.）白藜芦醇合酶基因PcRS 的功能，以转PcRS 基因拟
南芥为材料，对其进行分子鉴定和表型研究。利用Southern blot 和Northern blot 验证了外源PcRS 基因在转基因拟南
芥基因组中的整合和表达。对经过选育得到的T3代纯合株系（L4、L6、L15）进行性状观察。在体视显微镜下观察“生
型和转基因拟南芥植株表型曰采用分光光度法测定低氮培养基上的子叶花青素含量。结果显示，与“生型拟南芥相
比，转基因拟南芥的种皮颜色发生改变呈浅黄褐色，并且子叶中花青素含量显著减少。这表明PcRS 基因可能导致转
基因拟南芥的黄酮类物质合成受阻。 |
| 英文摘要: |
| To screen transgenic Arabidopsis plants with Polygonum cuspidatum resveratrol synthase (PcRS), and to
analyze the biological function of PcRS in the transgenic Arabidopsis plants, the integration and expression of transgene in
the genome of Arabidopsis was confirmed by Southern blot and Northern blot analyses. With stereomicroscope, the plant
phenotypes were observed in transgenic Arabidopsis and wild-type Arabidopsis. Arabidopsis plants were grown on medium
devoid of nitrogen sources and anthocyanin content was quantitated by using a spectrophotometer. The homozygous T3
progenies of three transgenic lines (L4, L6 and L15) were obtained. In this paper, we observed that transgenic plants had
seeds with a pale fawn colour and accumulated less anthocyanin in cotyledons in contrast to the wild -type plants.
Overexpression of PcRS could cause altered seed coat pigmentation and significant reduction in anthocyanin levels. These
results suggested that additional PcRS activities lead to the disturbance of the subsequent flavonoid biosynthesis steps in
Arabidopsis. |
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