| 王栓群， 孙瑞芹， 刘燕玲，等.华南地区猪流行性腹泻病毒的分子流行病学调查与分析[J].广东农业科学,2015,42(6):91-96 |
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华南地区猪流行性腹泻病毒的分子流行病学调查与分析 |
| Investigation and analysis on molecular epidemiology ofporcine epidemic diarrhea virus in south China |
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| DOI： |
| 中文关键词: 猪流行性腹泻病毒 分子流行病学 基因进化分析 |
| 英文关键词: porcine epidemic diarrhea virus molecular epidemiology gene evolution analysis |
| 基金项目: |
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| 摘要点击次数: 1450 |
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| 中文摘要: |
| 为了解华南地区猪流行性腹泻病毒（PEDV）的流行、变异及进化情况，从该地区的不同猪场采集
2 159 份样品，利用 RT-PCR 方法检测 PEDV，扩增了部分 PEDV 阳性样品中的 S、M 和 ORF3 基因并测序，利用
生物信息学软件进行序列分析。 结果显示，PEDV 在猪场中的检出率为 100%， 在所有样品中的平均检出率为
53.0%，在哺乳仔猪、断奶仔猪、育肥猪和母猪样品中，PEDV 平均阳性率分别为 67.6%、42.8%、12.8%和 47.2%。
根据 S、ORF3 和 M 基因建立的进化树，PEDV 可分为 2 个基因群，其中当前样品株为一群，韩国、美国和中国的
参考毒株为一群。 结果表明，PEDV 在华南地区猪场普遍存在，其基因也在不断发生变化。 |
| 英文摘要: |
| In order to know the epidemic, variation and evolution of porcine epidemic diarrhea virus (PEDV) in
south China, 2159 samples were collected from different herds in south China. In this study, partial spike (S), ORF3,
and membrane (M) genes amplified from these samples were sequenced and analyzed. While, the morbidity and
mortality rate of herds mentioned above was counted. The results showed that all of the herds were positive for
PEDV, the average positive rate of PEDV was 53.0%. However, the mean positive rate of PEDV in suckling piglets,
nursing piglets, finishing pigs and sows were 67.6%，42.8%，12.8% and 47.2%, respectively. The phylogenetic trees
that were constructed by MEGA based on partial S, M and ORF3 genes, showed that all the PEDV analyzed fell into
two groups, which were consisted by present fields strains and reference strains. These results indicated that PEDV
was prevalent in the field herds in south China and variant in PEDV genes. |
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