| 张大智，詹儒林，柳 凤，等.芒果细菌性角斑病菌细胞壁降解酶反应条件优化…[J].广东农业科学,2015,42(8):66-71 |
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芒果细菌性角斑病菌细胞壁降解酶反应条件优化… |
| Reaction conditions optimization of cell wall degradingenzymes from mango bacterial leaf spot pathogen |
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| DOI： |
| 中文关键词: 芒果细菌性角斑病 细胞壁降解酶 反应条件 酶活性 |
| 英文关键词: mango bacterial leaf spot cell wall degrading enzymes reaction condition enzyme activity |
| 基金项目: |
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| 摘要点击次数: 1417 |
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| 中文摘要: |
| 为优化出芒果细菌性角斑病菌细胞壁降解酶酶活性的反应条件，采用3，5- 二硝基水杨酸法（DNS
法），分别测定以1%羧甲基纤维素钠（CMC）、0.5%CMC+0.5%果胶和1%果胶为诱导底物的细胞壁降解酶活性，
并从温度、时间、pH等方面优化酶活性测定条件。结果表明在3种主要细胞壁降解酶中，羧甲基纤维素酶（Cx）
以1.0%…CMC 作为底物诱导效果最好，多聚半乳糖醛酸酶（PG）和果胶甲基半乳糖醛酸酶（PMG）以1.0% 柑橘
果胶作为底物诱导效果最好。Cx 和 PG 的最适反应温度分别为 60℃和 50℃，最适反应时间分别为 60…min 和
20…min，当pH分别达到4.6和6.6时，Cx酶和PG的酶活性最强。 |
| 英文摘要: |
| To optimize the reaction conditions of cell wall degrading enzymes from bacterial leaf spot pathogens ofMangifera indica Linn（mango），3，5-dinitrosalicylic acid method（DNS method）was used to determine the activitiesof cell wall degrading enzymes（CWDEs）with the induction substrate of 1% sodium carboxymethyl cellulose（CMC），0.5% CMC+0.5% pectin and 1% pectin，respectively；and these CWDEs assay conditions in terms of temperature，time，pH，ect were optimized. The results showed that，1.0% CMC was the most suitable substrate for the induction ofcarboxymethyl cellulose（Cx），whereas 1.0% pectin was most suitable for the induction of polygalacturonase（PG）andpectin methylgalactuionase（PMG）. The activities of Cx and PG were the highest at 60℃ and 50℃，respectively，for60 min and 20 min，respectively；the activities of Cx and PG were the highest when the pH value reached 4.6 and 6.6，respectively. |
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