| 陈冠州，沈会芳，林壁润，等.鬼针草白粉病的病原菌鉴定[J].广东农业科学,2015,42(11):77-79 |
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鬼针草白粉病的病原菌鉴定 |
| Pathogen identification of Bidens pilosa powdery mildew |
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| DOI： |
| 中文关键词: 鬼针草 白粉病 病原鉴定 二孢白粉菌 |
| 英文关键词: Bidens pilosa powdery mildew pathogen identification Golovinomyces cichoracearum |
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| 摘要点击次数: 1665 |
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| 中文摘要: |
| 采用形态学鉴定、致病性鉴定和分子系统学鉴定方法对中国广东地区首次发现的鬼针草白粉病进行
病原鉴定研究。结果表明，该病原菌分生孢子梗直立，圆柱形，没有分支，大小为100~160 μm × 11~13 μm，包含1
个足细胞，2~4 个短细胞；分生孢子串生，圆柱形，大小为20~50 μm × 10~20 μm（长/ 宽为 1.25~3.0）；病原菌接种
叶片病症与自然状态一样，形成薄而边缘不明显的白色斑；对其核糖体DNA 内转录间隔区（ITS）序列进行扩增、
测序获得528 bp 序列（GenBank 登录号：KJ920100），经MECA 3.1 软件分析，其与来自二孢白粉菌（Golovinomyces
cichoracearum）的KF453969 序列聚为一枝，与其相似度达到99%。本研究表明，广州地区的鬼针草白粉菌为二孢
白粉菌（G. cichoracearum）。 |
| 英文摘要: |
| In order to make clear the phylogenetic evolution relationship of Bidens pilosa powdery mildew detectedin Guangzhou，China，morphological，etiological and molecular systematic identification were tested. Results show thatconidiophores were measured with 100 - 160 μm × 11 - 13 μm，and they produced 2 to 4 immature conidia in chainswith a crenate outline and 1 to 2 straight cells of the conidiophore at the terminal. Conidia were oval-shaped to roundshaped，hyaline，and measured with 20 - 50 μm × 10 - 20 μm（length/width ratio of 1.25 - 3.0）. The fungus appearingon the inoculated plants had the same morphology with those from original diseased plants. Internal transcribed spacer（ITS）sequences of its ribosomal DNA were amplifild by PCR， 528 bp sequences（GenBank：KJ920100） were obtained，and sequence analysis by MEGA3.1 software revealed that sequence of pathogenic bacteria and KF453969 fromGolovinomyces cichoracearum gather together with 99% of similarity. The results show that pathogen of B. pilosa powderymildew in Guangzhou is G. cichoracearum. |
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