| 宋西红，郝 磊，吕晓玲，等.紫苏肉桂酸4-羟基化酶基因的克隆与表达[J].广东农业科学,2015,42(11):124-129 |
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紫苏肉桂酸4-羟基化酶基因的克隆与表达 |
| Cloning and expression analysis of cinnamate 4-hydroxylasegene from Perilla frutescens |
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| DOI： |
| 中文关键词: 紫苏 迷迭香酸 肉桂酸4- 羟基化酶 基因克隆 表达分析 |
| 英文关键词: Perilla frutescens rosmarinic acid cinnamate 4-hydroxylase gene cloning expression analysis |
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| 摘要点击次数: 1392 |
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| 中文摘要: |
| 为阐述紫苏中迷迭香酸合成的分子调节机制，应用同源克隆和RACE 技术首次从紫苏叶片中克隆得
到肉桂酸-4- 羟基化酶基因（PerC4H）的全长cDNA 序列（GenBank 登录号：KM434189）。PerC4H 基因总长度为
1 667 bp，基因的开放阅读框（ORF）为1 518 bp，编码505 个氨基酸残基，还含有长度为21 bp 的5′非编码区（5′
UTR）和95 bp 的3′非编码区（3′UTR）。系统进化树分析得到PerC4H 基因与唇形科植物丹参和黄芩的亲缘性最
近。由实时荧光定量PCR 分析可知，该基因在紫苏中具有组织表达特异性，根中的表达最高，茎中次之，而叶中最
少。一定浓度的赤霉素与茉莉酸甲酯能提高PerC4H 的表达量，而脱落酸和黑暗处理能降低PerC4H 的表达量。 |
| 英文摘要: |
| In order to clarify the molecular mechanism of romarinic acid（RA）biosynthesis，the cDNA of cinnamate4-hydroxylase（PerC4H）gene was firstly cloned from the leaves of Perilla frutescens（GenBank accession No：KM434189）by homology-based cloning and rapid amplification of cDNA ends（RACE）technique. The full-length ofPerC4H cDNA was 1 667 bp，containing 1 518 bp open reading frame（ORF）which encoded a pepitide of 505 amanioacids and two non-coding regions，21 bp 5′UTR and 95 bp 3′UTR. Phylogenetic tree analysis showed that PerC4Hhad the closest relationship with Salvia miltiorrhiza and Scutellaria baicalensis. Real time quantitative PCR showedthat expression of PerC4H was the highest in root，moderate in stem，and the lowest in leaf. Further expression analysisshowed that Gibberellins and MeJA treatments could up-regulate the transcript level of PerC4H，while ABA and darktreatments could down-regulate its transcription level in some degree. |
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