| 吕思行1，郑常格1，江敏华1，徐树兰1，杨思佳2，汤 历1，刘吉平2.一株昆虫病原性真菌莱氏野村菌的分离与鉴定[J].广东农业科学,2015,42(15):53-56 |
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一株昆虫病原性真菌莱氏野村菌的分离与鉴定 |
| Isolation and identification of Nomuraea rileyi，an entomopathogenic fungus |
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| DOI： |
| 中文关键词: 昆虫病原真菌 莱氏野村菌 分离 鉴定 |
| 英文关键词: entomopathogenic fungus：Nomuraea rileyi isolation identification |
| 基金项目:广东省重点科研基地建设项目（2012A
061300001）；广东省科技基础条件建设项目（2013B0605
00004，201509020002） |
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| 中文摘要: |
| 利用PDA 培养基，对收集自广东化州的家蚕病原真菌（编号为Ma1 菌株）进行分离培养。首先
对菌落特征、菌丝、分生孢子梗及分生孢子形态特征进行观察。再利用真菌核糖体基因ITS 区域设计合成的引
物对Ma1 菌株进行PCR 扩增得到558 bp 大小的目的条带。目的条带采用Blast 方法将测序结果在GenBank 中
进行同源搜索，依据邻接法构建获得与其相关菌株的ITS 序列系统发育树。通过形态学和ITS 基因序列分析，
最终判定来自家蚕的病原真菌Ma1 菌株为莱氏野村菌（Nomuraea rileyi） |
| 英文摘要: |
| A strain（No. Ma1）was isolated using PDA medium from the muscardine cadaver of silkworm collected
from Huazhou of Guangdong province. Its colony characteristics were observed and described. The morphological of
its mycelium，matured spores and conidiophores were observed under microscope and identified by molecular biology
techniques. The primers were designed according to PCR amplification area ITS of the ribosome genes from Ma1，it could
get amplification strip about 558 bp. We searched for homology using the BLAST programs available at the web site of the
National Center for Biotechnology Information，and the phylogenetic tree for the genus Nomuraea rileyi was constructed
by Neighbor-Joining method. Based on its morphology and ITS sequence analysis，the strain from entomopathogenic
fungus was identified as Nomuraea rileyi |
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