| 郭 宁，胡利宗，解恒昌，袁明月，王秋霞，周 琳，王红星.棉花与模式植物DGAT1 基因
的鉴定与比较分析[J].广东农业科学,2015,42(15):98-104 |
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棉花与模式植物DGAT1 基因
的鉴定与比较分析 |
| Identification and comparative analysis of DGAT1genes among cotton and other model plants |
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| DOI： |
| 中文关键词: 二酰甘油酰基转移酶 保守基序 棉花 同源基因 进化 |
| 英文关键词: diacylglycerol acyltransferase conserved motif cotton homologous gene evolution |
| 基金项目:2 0 1 5 年周口师范学院青年科研基金
（zknuB315213） |
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| 摘要点击次数: 1251 |
| 全文下载次数: 528 |
| 中文摘要: |
| 二酰甘油酰基转移酶（DGAT）是催化三脂酰甘油生物合成的关键酶。对棉花与其他植物DGAT1
基因进行鉴定与比较分析，序列分析结果表明：棉花与大多数模式植物DGAT1 基因有16 个外显子，内含子长
度高度变异，但其相位极其保守；这些基因编码的蛋白具有7 个保守基序，核心保守基序与功能结构域相互重
叠。系统进化分析显示：基因树与物种树具有较高的一致性，DGAT1 基因进化能真实反映物种系统发生关系；
棉花DGAT1 基因以单拷贝形式存在，但在玉米、水稻、杨树与小立碗藓中均存在至少两个DGAT1 基因。选择
压力检测结果显示：除了PtDGAT1a/PtDGAT1b 受制于正选择作用外，剩余4 对同源基因均受控于纯净选择；
在基因亚群水平共检测到17 个正选择位点，这说明在显著水平下各个亚群中均未检测到正选择位点。这些结
果可为进一步研究棉花及植物DGAT1 基因的功能提供基础。 |
| 英文摘要: |
| Diacylglycerol acyltransferases（DGATs） are the key enzymes that catalyze the triacylglycerol
biosynthesis. The comparative analysis was performed on the DGAT1 genes of cotton and other model plants. Sequence
analysis showed that most of the DGAT1 genes，with high variation of intron length and high conservation phrase of
intron，from the cotton and other model plants had 16 exons. Additionally，7 conserved motifs were present in these
DGAT1 proteins. The core motifs were overlapped with the functional domain of DGAT1 protein. Phylogenetic analysis
demonstrated that gene tree was highly consistent to species tree，suggesting that the evolutionary history of species
was revealed by gene tree. There was single copy of DGAT1 gene in cotton，but at least two duplicated DGAT1 genes
were identified in rice，maize，poplar and moss genomes. The selective pressure analysis showed that the PtDGAT1a/
PtDGAT1b was under positive selection，but four pair homologous genes were under negative selection. 17 positively
selected sites were identified at subgroup level（P<0.05），suggesting these subgroup under relaxed functional
constraint. The findings provide a basis of function and evolution of DGAT1 genes in cotton and other model plants. |
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