| 王冰峰1，2，王宏芝2，魏建华2.杨树转录因子PtoMYB057 的功能分析[J].广东农业科学,2015,42(15):105-109 |
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杨树转录因子PtoMYB057 的功能分析 |
| Analysis of function of PtoMYB057transcription factor from poplar |
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| DOI： |
| 中文关键词: 转录因子 木质部 亚细胞定位 |
| 英文关键词: transcriptional factor xylem subcellular localization |
| 基金项目:国家高技术研究发展计划项目（2011AA
100201，2012CB114501） |
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| 摘要点击次数: 1272 |
| 全文下载次数: 526 |
| 中文摘要: |
| 转录因子在转录水平上调控目的基因的表达是植物对其生长调控的一种重要方式。以杨树为材
料，克隆了杨树PtoMYB057 转录因子的全长cDNA。荧光定量PCR 的分析表明，PtoMYB057 在木质部高水平
表达。系统进化分析表明，PtoMYB057 与拟南芥MYB006、MYB008、MYB003、MYB007、MYB108 相似性较
高。根据亚细胞定位结果，证实了PtoMYB057 定位于细胞核中。在烟草中过量表达PtoMYB057 基因的试验表
明，此基因对烟草的生长以及茎发育有影响。 |
| 英文摘要: |
| It is an important way that transcription factors regulate the expression of target genes at the
transcriptional level，to regulate plant growth and development. In this study，we cloned the full-length cDNA of
PtoMYB057 transcription factor in poplar. The results of fluorescent quantitative PCR showed that the transcript of
PtoMYB057 was the most abounded in xylem. Phylogenetic analysis showed that PtoMYB057 had higher similarity with
Arabidopsis MYB006，MYB008，MYB003，MYB007，MYB108. Subcellular localization showed that PtoMYB057 was
localized in nucleus. Overexpression of PtoMYB057 in transgenic tobacco indicated that this gene played important roles
in plant growth and development. |
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