| 章锦涵1，张 威1，李美欣1，陈蓉蓉1，赵晓娟1，刘 佳2，白卫东1，陈海光1.检测牛奶中氯霉素的双通道
分子印迹传感器研究[J].广东农业科学,2015,42(19):95-100 |
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检测牛奶中氯霉素的双通道
分子印迹传感器研究 |
| Study on dual channel molecularly imprinted sensorfor chloramphenicol detection in milk |
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| DOI： |
| 中文关键词: 分子印迹 电化学传感器 氯霉素 聚邻苯二胺 丝网印刷电极 |
| 英文关键词: m o l e c u l a r l y i m p r i n t e d electrochemical sensor chloramphenicol poly
（o-phenylenediamine） screen-printed electrode |
| 基金项目:广东省自然科学基金（S2013010013416）；
广东省高等学校优秀青年教师培养计划项目（ Y q
2 0 1 3 0 9 7 ）；广州市软科学研究计划项目（ 2 0 1 4 Y
4300007） |
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| 摘要点击次数: 1258 |
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| 中文摘要: |
| 以氯霉素（CAP）为模板分子，邻苯二胺（o-PD）为功能单体，采用循环伏安法（CV）分别在
玻碳电极（GCE）和双通道丝网印刷碳电极（SPCE）表面原位电聚合形成聚邻苯二胺膜，经醋酸- 乙醇混合
溶液将模板分子洗脱，制得两种氯霉素分子印迹膜电极（CAP-MIP/GCE 和CAP-MIP/SPCE）。以K3Fe（CN）6 为
电活性探针，建立了间接测定氯霉素的分析方法，并对分子印迹传感器的重现性和选择性等进行评价。CAPMIP/
GCE 和CAP-MIP/SPCE 在溶液中的响应电流与氯霉素浓度分别在1.0×10-5~1.0×10-4 mol/L、2.0×10-5~1.4
×10-4 mol/L 范围内呈良好的线性关系，检出限分别为5.2×10-6、1.9×10-6 mol/L。运用建立的方法对市售纯
牛奶中的氯霉素进行测定，并用紫外分光光度法进行了验证。结果表明，该氯霉素传感器选择性好、准确可
靠，适用于牛奶样品中氯霉素的测定，其中双通道分子印迹传感器可同时平行检测样品两次，且样品用量少
（50 μL），在分析速度和重现性等方面比基于玻碳电极的分子印迹传感器都获得了较大的提升。 |
| 英文摘要: |
| Taking chloramphenicol（CAP）as template molecule，and o-phenylenediamine（o-PD）as
functional monomer，poly（o-phenylenediamine）（PPD）film was electropolymerized on the surface of a glassy
carbon electrode（CAP-PPD/GCE）and a dual channel screen-printed carbon electrode（CAP-PPD/SPCE）
using cyclic voltammetry（CV），respectively. Furthermore，CAP molecularly imprinted electrode（CAP-MIP/GCE
and CAP-MIP/SPCE）was fabricated by eluting the template molecules using the mixed solution of acetic acid and
alcohol. CAP was indirectly determined based on potassium ferricyanide as electroactive probe．The properties of
molecularly imprinted sensor were investigated. The response current of the two sensor had good linear relationship
with the concentration of CAP in the range of 1.0×10-5-1.0×10-4 mol/L and 2.0×10-5-1.4×10-4 mol/L. The
detection limit was 5.2×10-6 mol/L and 1.9×10-6 mol/L，respectively. The proposed method was applied to detect
CAP in commercial pure milk，and ultraviolet spectrophotometry was used to verify. The results showed that the
proposed sensor had the advantages of good selectivity
and high accuracy，and was suitable for determination
of CAP in milk samples. Compared to CAP-MIP/GCE，
the dual channel sensor could achieve two repetitive
measurements by using a small amount of sample（50
μL），furthermore，test efficiency and reproducibility
were obviously improved |
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