| 宋华伟，刘 颖，曹荣祥，张巨明.ZS-SJZ 结缕草与60Co-γ 辐射诱变
新品系的抗寒性比较[J].广东农业科学,2015,42(20):39-44 |
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ZS-SJZ 结缕草与60Co-γ 辐射诱变
新品系的抗寒性比较 |
| Comparison of cold resistance of ZS-SJZ zoysiagrassand its new strains induced by 60Co-γ radiation |
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| DOI： |
| 中文关键词: ZS-SJZ 结缕草 60Co-γ 辐射 新品系 隶属函数 抗寒性 |
| 英文关键词: ZS-SJZ zoysiagrasses 60Co-γ radiation new strains subordinate function cold resistance |
| 基金项目:广东省科技计划项目（2012B020302002） |
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| 中文摘要: |
| 采用低温（5、10、20℃）处理ZS-SJZ 结缕草（ZSS）及60Co-γ 辐射诱变后筛选的2 个新品
系（ZSS-50-1 和ZSS-60-2），通过测定叶片脯氨酸含量、相对电导率、可溶性总糖含量、超氧化物歧化酶
（SOD）活性和丙二醛（MDA）含量等5 个生理指标，同时观测草坪草颜色和盖度变化，比较3 种材料之间
抗寒性强弱。结果表明：随着胁迫温度的降低，3 种材料的脯氨酸含量、叶片相对电导率、可溶性总糖含量、
MDA 含量和SOD 活性均呈增大趋势，草坪颜色评分和盖度降低。在5℃低温胁迫下，新品系的脯氨酸含量、
叶片相对电导率、可溶性总糖含量、MDA 含量、颜色评分和盖度高于ZSS，而SOD 活性低于ZSS。利用隶属
函数标准差系数赋予权重法对3 种材料的抗寒性进行综合评价，抗寒性由强到弱依次为ZSS-50-1 ＞ ZSS ＞
ZSS-60-2。 |
| 英文摘要: |
| Study on cold resistance of ZS-SJZ zoysiagrass and its two new strains induced by 60Co-γ radiation，
ZSS-50-1 and ZSS-60-2 was conducted under low temperature at 5℃，10℃，20℃ respectively. The cold resistance of
the three zoysiagrasses was evaluated by measuring proline content，relative electrical conductivity，soluble total sugar
content，superoxide dismutase（SOD）activity and malondialdehyde（MDA）content of leaves，and the turfgrass
coverage and color were observed. The results showed that the proline content，leaf relative electrical conductivity，
soluble sugar content，MDA content and SOD activity of the three zoysiagrasses showed an higher trend with the
decrease of temperature stress，whereas the turfgrasses coverage and color ratings were reduced. Under 5℃ treatment，
the proline content，relative electrical conductivity，soluble total sugar content and MDA content of the new turfgrass
strains were greater than those of ZSS zoysiagrass，while the SOD activity was lower than that of ZSS zoysiagrass. Using
the method of subordinate function weights coefficient of standard deviation the cold resistance of the three zoysiagrasses
was comprehensively evaluated，the order of cold resistance ability was ZSS-50-1 ＞ ZSS ＞ ZSS-60-2. |
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