| 张 来，杨碧昌，黄元射.黔产竹节参根人参皂苷提取工艺
与RP-HPLC 含量分析[J].广东农业科学,2015,42(20):86-90 |
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黔产竹节参根人参皂苷提取工艺
与RP-HPLC 含量分析 |
| Extraction process and RP-HPLC analysis of ginsenosidesfrom Panax japonicus root in Guizhou |
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| DOI： |
| 中文关键词: 竹节参 人参皂苷 提取工艺 RP-HPLC |
| 英文关键词: Panax japonicus ginsenoside extraction process RP-HPLC |
| 基金项目:国家自然科学基金（81102796）；贵州省
优秀青年科技人才专项（黔科合人字［2015］18 号）；贵
州省中药现代专项（黔科合中药字［2011］50 号）；贵州
省教育厅重点项目（黔教高发2011［278］号） |
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| 中文摘要: |
| 利用正交试验设计黔产竹节参的提取工艺技术和RP-HPLC 建立多种人参皂苷的检测方法，结
果发现，黔产竹节参根人参皂苷的最优提取工艺为：材液比1∶10，甲醇浓度65%，提取温度50℃；在波长
203 nm 下，Re、Rg1、Rg2、Rh1、Rh2 的线性回归方程依次为y=42.08x+24.25、y=52.23x+43.87、y=40.09x+3.51、
y=115.36x+32.41、y=98.46x+30.52，线性范围和相关系数在0.18~15.45 μg 和0.996~0.998 之间，仪器重现性好，
稳定、精密度和加样回收率高；人参皂苷单体含量随着竹节参生长年限的增加而提高；除Rg2 外，5 年生竹节
参的Re、Rg1、Rh1、Rh2 含量均超过1 mg/g；在相同年限的不同生长时期，Re 含量均高于其他4 种，可见Re 为
黔产竹节参根的标志性人参皂苷。 |
| 英文摘要: |
| With orthogonal experiments，the extraction process was optimized，and by RP-HPLC，the detection
method was established for ginsenosides from Panax japonicus root in Guizhou. The results showed that the optimal
extraction process of ginsenosides from P. japonicus root was as follows：ratio of material to liquid with 1∶10，methanol
concentration of 65%，at 50℃. At the wavelength of 203 nm，the linear regression equation of Re，Rg1，Rg2，Rh1 and
Rh2 were y=42.08+24.25x，y=52.23+43.87x，y=40.09+3.51x，y=115.36+32.41x，y=98.46+30.52x，respectively，
the linear range and correlation coefficient were 0.18-15.45 μg and 0.996-0.998，indicating that the method had good
reproducibility，stability，high precision and sample recovery rate. The content of ginsenosides monomer increased with
the growth period of P. japonicus increasing. Except for Rg2，the contents of other four kinds of ginsenosides monomer
were more than 1 mg/g in five-year P. japonicus root. At different growth period of the same year，the content of Re was
higher than that of the other four，indicating that Re was the main ginsenoside from P. japonicus root in Guizhou |
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