| 梁冬秋，张翔宇，张 松.白蜡多年菌液体培养产胞外多糖条件的研究[J].广东农业科学,2015,42(20):97-101 |
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白蜡多年菌液体培养产胞外多糖条件的研究 |
| Submerged culture conditions for exopolysaccharideproduction by Perenniporia fraxinea |
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| DOI： |
| 中文关键词: 白蜡多年菌 液体培养 胞外多糖 条件优化 |
| 英文关键词: Perenniporia fraxinea submerged culture exopolysaccharide condition optimization |
| 基金项目:华南师范大学学生课外科研项目（1 2
SKGB04） |
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| 摘要点击次数: 1197 |
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| 中文摘要: |
| 通过单因素及正交试验研究了白蜡多年菌液体培养产胞外多糖适宜的碳源、氮源及培养条件，为
白蜡多年菌的开发利用及其多糖工业化生产提供试验依据。单因素试验结果表明，白蜡多年菌液体培养产胞
外多糖的适宜碳源为蔗糖，最适浓度为40 g/L，适宜氮源为硝酸铵，最适浓度为1 g/L。正交试验结果表明，白
蜡多年菌液体培养产胞外多糖的最优条件：最适初始pH 为5.5、最佳装液量为125 mL、最适温度为27℃、最
适转速为150 r/min、最适培养时间为9 d。在此最适培养条件下，菌丝胞外多糖的产量比优化前提高55.6%。 |
| 英文摘要: |
| Shake flask culture was used and the yield of exopolysaccharide（EPS）was taken as main index
to ensure the optimum culture conditions for submerged fermentation of Perenniporia fraxinea. The carbon-source，
nitrogen-source and cultivation conditions of P. fraxinea in submerged culture were optimized by single factor
experiments and multi factor orthogonal experiments respectively. The results showed that the optimal carbon source was
surge（40 g/L）and nitrogen source was ammonium nitrate（1 g/L）. In addition，the optimal temperature，initial pH，
bottled fluid volume，shaking speed and agitation time for cultivation of P. fraxinea were 27℃， pH 5.5，125 mL，150
r/min，and 9 days. Under the optimal culture conditions，the yield of EPS significantly increased by 55.6% compared
with no optimization. |
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