| 杨云龙，蔡征文，胡开辉.废弃平菇栽培料纤维素酶提纯工艺研究[J].广东农业科学,2015,42(21):102-105 |
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废弃平菇栽培料纤维素酶提纯工艺研究 |
| Extraction and purification of cellulase fromwaste Pleurotus ostreatus compost |
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| DOI： |
| 中文关键词: 平菇 废弃栽培料 酶活力 纤维素酶 分离纯化 |
| 英文关键词: Pleurotus ostreatus waste compost enzyme activity cellulase separation and purification |
| 基金项目:福建省教育厅项目（JA14122） |
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| 中文摘要: |
| 为充分利用废弃物资源，以废弃平菇栽培料为原料，经浸提正交优化、硫酸铵分级沉淀、透析、双
水相萃取等方法，提取、加工高纯度纤维素酶制剂。结果表明：以0.2 mol/L pH 5.5 的乙酸- 乙酸钠为缓冲液，
料液比为 1∶4，温度15℃，浸提 1~2 h，再利用质量分数均为20% 的PEG6000- 硫酸铵双水相体系进行萃取，
纯化后的纤维素酶比活力为19 705.60 U/mg，纯化倍数为5.34，回收率为60.9%。 |
| 英文摘要: |
| In order to take full use of waste resources，the waste Pleurotus ostreatus compost was selected to extract
and process high-purity cellulase by orthogonal optimization，ammonium sulfate precipitation，dialysis and aqueous
two-phase extraction method. Results showed that the specific activity，purification fold and recovery rate of the resultant
enzyme was 19 705.60 U/mg，5.34 and 60.9%，respectively，after using 0.2 mol/L acetic acid-sodium acetate as buffer
solution（pH 5.5），ratio of material to liquid at 1∶4，immersing for 1-2 h at 15℃ and extraction by aqueous twophase
system composed of PEG6000 and ammonium sulfate with mass fraction of 20%. |
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