| 许 龙1，黄运安1，朱秋劲1，2，汪瑞敏1，叶 春1
（1. 贵州大学酿酒与食品工程学院，贵州 贵阳 550025；
2. 贵州大学食品科学工程研究中心，贵州 贵阳 550025）.天麻多糖的提取及其清除自由基作用研究[J].广东农业科学,2015,42(21):117-123 |
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天麻多糖的提取及其清除自由基作用研究 |
| Extraction and scavenging free radical effects ofpolysaccharides from Gastrodia elata |
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| DOI： |
| 中文关键词: 响应面法 天麻多糖 清除自由基 |
| 英文关键词: response surface methodology Gastrodia elata polysaccharides scavenging free radical |
| 基金项目:国家自然科学基金（31360373） |
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| 摘要点击次数: 1205 |
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| 中文摘要: |
| 基于单因素试验结果，利用响应面法（RSM）优化天麻多糖的超声波提取工艺，依据回归分析
确定多糖提取率的影响因子，求得最佳浸提条件为：水料比40∶1，超声波输出功率75.70%，超声时间29.32
min。采用分光光度法和化学发光法比较天麻多糖与3 种天然抗氧化剂α- 硫辛酸、Vc 和VE 对二苯代苦味肼
基自由基（DPPH·）、超氧阴离子自由基（O2
-·）、羟自由基（·OH）、过氧化氢（H2O2）以及过氧亚硝基阴离
子（ONOO-）的清除能力。结果表明，在0~500 μg/mL 质量浓度范围内，天麻多糖对DPPH·的清除率和α-
硫辛酸的清除效果接近，强于VE，明显弱于Vc，最高清除率达到22.37%；对O2
-·的清除能力强于Vc 和VE，
弱于α- 硫辛酸，最高清除率达到12.23%；对·OH 的清除能力弱于Vc，在400~500 μg/mL 质量浓度范围
内清除率与α- 硫辛酸、VE 接近，最高清除率达到4.85%；对H2O2 的清除能力强于α- 硫辛酸，略弱于VE，
明显弱于Vc，最高清除率达到25.80%；对ONOO- 的清除能力明显弱于α- 硫辛酸和Vc，最高清除率达到
20.52%。 |
| 英文摘要: |
| Response surface methodology（RSM）was applied to optimize the extraction conditions for
polysaccharides from Gastrodia elata based on single factor tests. Technological condition parameters were determined
according to regression analysis. The optimum conditions were as follows：ratio of water to material was 40：1，ultrasonic
power output was 75.70%，extraction time was 29.32 min. Spectrophotometry and chemiluminescence were employed to
compare the abilities of scavenging free radicals including DPPH·，O2
-·，·OH，H2O2 and ONOO- of polysaccharides
extracted from G. elata and three natural antioxidants α-lipoic acid，Vc and VE. The results showed that at the
concentration of 0-500 μg/mL，the scavenging ability of polysaccharides extracted from G. elata on DPPH· was similar
to that of α-lipoic acid，stronger than that of VE，obviously weaker than that of Vc，the highest clearance rate reached
12.23%；the scavenging ability on ·OH was weaker than that of Vc，similar to that of α-lipoic acid and VE at the
concentration of 400-500 μg/mL，the highest clearance rate reached 4.85%；the scavenging ability on H2O2 was stronger
than that of α-lipoic acid，slightly weaker than that of VE，obviously weaker than that of Vc，the highest clearance rate
reached 25.80%；the scavenging ability on ONOO- was obviously weaker than that of Vc and α-lipoic acid，the highest
clearance rate reached 20.52%. |
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