王茂胜1,2,汪汉成2,马 莹3,郭亚利3,胡向丹3,黄 鸿3,蒋选利1.烟草内生细菌多样性研究[J].广东农业科学,2015,42(23):59-64 |
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烟草内生细菌多样性研究 |
Reasearch on endophytic bacterial diversity in tobacco |
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DOI: |
中文关键词: 烟草 内生细菌 多样性 16S rDNA |
英文关键词: tobacco endophytic bacteria diversity 16S rDNA |
基金项目:国家自然科学基金(31360448);贵州省
烟草公司黔西南州公司科技项目(2013-09) |
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中文摘要: |
为了揭示烟草内生细菌多样性,选择K326、云85 和红花大金元3 个烤烟品种进行内生细菌的
分离和16S rDNA 序列分析鉴定,获得不同品种烟草内生细菌的多样性特征。结果显示,27 株内生细菌分
别属于枯草芽孢杆菌Bacillus subtilis(33%)、蕈状芽孢杆菌Bacillus mycoides(4%)、芽孢杆菌属Bacillus
sp.(11%)、肠杆菌属Enterobacter sp.(4%)、寡养单胞菌Stenotrophomonas sp.(4%)、热带根瘤菌Rhizobium
tropici(4%)、假单胞菌属Pseudomonas sp.(4%)、土地芽孢杆菌属Terribacillus sp.(4%),芽孢杆菌属
Bacillus sp. 为优势细菌类群。3 个品种的 Shannon index(H′)以K326 最高、为2.02,其次是云85、为1.48,红
花大金元最低、为1.3。 |
英文摘要: |
The diversity of endophytic bacteria in tobacco tissues originating from three flue-cured tobacco
varieties(K326,YUN 85 and Honghuadajinyuan)in Guizhou province was surveyed,to describe the distribution
of isolated endophyte species in different varieties,and to assess their specificity and abundance. Endophytic
bacteria were isolated from surface-sterilized tissues,and the strains were identified based on molecular
characteristics of analysis of the 16S rDNA sequence. 27 strains belonged to eight different taxa,they were Bacillus
subtilis(33%),Bacillus mycoides(4%),Bacillus sp.(11%),Enterobacter sp.(4%),Stenotrophomonas
sp.(4%),Rhizobium tropici(4%),Pseudomonas sp.(4%),Terribacillus sp.(4%),dominated by Bacillus
sp. Shannon Wiener diversity index of K326,YUN 85 and Honghuadajinyuan(HD)were 2.02,1.48 and 1.3
respectively. |
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