| 张 琳1，2，程 萍1，2，喻国辉2，王 燕2，3.枯草芽胞杆菌TR21激发的广粉1号根系抗性信号物质累积[J].广东农业科学,2016,43(5):101-106 |
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枯草芽胞杆菌TR21激发的广粉1号根系抗性信号物质累积 |
| Accumulation of resistance signal substances in Guangfen No.1（ Musa spp. ABB） roots induced by Bacillus subtilis strain TR21 |
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| DOI：10.16768/j.issn.1004-874X.2016.05.020 |
| 中文关键词: 香蕉枯萎病 枯草芽胞杆菌 诱导抗性 信号物质 |
| 英文关键词: Fusarium oxysporum f. sp. cubense Bacillus subtilis induced resistance signal substances |
| 基金项目:仲恺农业工程学院研究生创新项目（KA151495007）；2012 年省级农业科技推广项目（粤财农[2013]185 号） |
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| 中文摘要: |
| 通过检测广粉1 号根系中与抗性途径有关的信号物质NO、H2O2 和SA 含量变化，揭示枯草芽胞杆菌TR21 菌株诱导的粉蕉系统抗性与SAR 途径的关系。试验设置清水（CK）、TR21 叶腋接种、香蕉枯萎病菌FOC004 菌株根系接种和挑战接种（TR21 叶腋接种24 h 后再根系接种FOC004）4 种方式处理广粉1 号种苗，测定4 种方式接种后0、12、24、48、72、96 h 根系SA、NO、H2O2 含量。结果显示：TR21 处理和FOC004处理均在早期避免激活植物产生NO，而挑战处理则迅速激活植物根系产生NO 并持续维持高水平；TR21 处理和FOC004 处理也在早期避免迅速激活植物产生H2O2，FOC004 处理在24 h 才显著激发植物根系H2O2 含量升高，而TR21 处理需要到48 h 才使根系H2O2 达到最高，但挑战处理在12 h 就显著激发了植物根系的H2O2，并一直维持较高水平；TR21 处理早期显著激发植物根系游离SA 的产生并避免植物产生结合态SA，FOC004 处理则抑制植物根系在应答早期产生游离态和结合态SA，挑战处理主要激发植物长期稳定高水平产生结合态SA。试验表明，TR21 叶腋接种的确可以激发广粉1 号后期快速应答病原菌的入侵，对TR21 进一步开发和大田生产应用具有意义。 |
| 英文摘要: |
| In order to reveal the relationship between systemic acquired resistance（ SAR） and systemic resistance
in dwarf banana Guangfen No.1（ Musa sp. ABB） that induced by Bacillus subtilis strain TR21，the signal substances，
NO，H2O2 and SA in the roots of Guangfen No.1 were detected. The seedlings were treated by four treatments as control
（water inoculated in leaf axils），TR21 inoculated in leaf axils，Fusarium oxysporum f. sp. cubense strain FOC004microconidia inoculated on roots and challenge inoculation（ TR21 vaccinated leaf axils，24 h later FOC004 inoculated on roots），and then the SA，NO，and H2O2 content in roots were detected at 6 time points after 0 h，12 h，24 h，48 h，72 h and 96 h. Results showed that，both of the TR21 and FOC004 treatments avoided activating the plant to produce NO in early
stage，but challenge inoculation activated the plant roots producing NO quickly and maintaining a high level for long time. TR21 and FOC004 avoided activating the plant generating H2O2 quickly in early stage，FOC004 only
stimulated the H2O2 increasing markedly in roots at 24 h，but H2O2 in TR21 treated kept low level until 48 h and reached
the highest level，the challenge inoculation treatment significantly stimulated the H2O2 increasing at the 12 h，and
remained at a high level. TR21 treatment only promoted the production of free SA，but avoided bound SA observably in
the early stage，FOC004 treatment inhibited the both free and bound SA production，but challenge inoculation motivated
the plant producing bound SA for a long-term and stable level. To sum up，TR21 inoculation in leaf axils early could
indeed help the Guangfen No.1 response the invasion of pathogen quickly，which would help the further development
and field application of TR21. |
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