| 张卫娜，陈中健，吴秀菊，朱 翠，李秀梅，梁 锐，陈 庄.应用激光共聚焦显微技术检测镉对植物根系生长的影响[J].广东农业科学,2016,43(8):69-73 |
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应用激光共聚焦显微技术检测镉对植物根系生长的影响 |
| Effects of cadmium on plant root growth by laser confocal microscopy technique |
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| DOI：10.16768/j.issn.1004-874X.2016.08.012 |
| 中文关键词: 激光共聚焦显微技术 活性氧 细胞活性 钙离子 |
| 英文关键词: laser confocal microscopy technique ROS cell viability Ca2+ |
| 基金项目:广东省主体科研机构创新能力建设专项（2011070703） |
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| 摘要点击次数: 1940 |
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| 中文摘要: |
| 为了能简单、直观、无损伤地检测重金属镉（Cd）对植物的毒害作用，应用激光共聚焦显微技术，以植物光照培养箱中的拟南芥组培苗为材料，探讨0、50、75、100、200 μmol/L CdCl2 浓度对拟南芥幼根生长的影响。结果表明，随着Cd 处理浓度的增大，拟南芥组培苗幼根的生长受到明显抑制，特别是Cd 浓度≥ 75 μmol/L 时，根长明显变短，根细胞中累积的活性氧（ROS）和钙离子（Ca2+）也逐渐增多，而根细胞活性有所下降。进一步研究发现Ca2+ 螯合剂可减轻ROS 的积累，而ROS 清除剂对于Ca2+ 的增加影响明显，表明植株在遭遇镉胁迫时，根部ROS 与Ca2+ 积累存在着一定的互作关系。该技术能有效研究重金属对植物生长发育的毒害，为进一步揭示重金属的毒害机理提供理论基础。 |
| 英文摘要: |
| In order to detect the toxicity of heavy metal cadmium（ Cd） on plant by a simple，straightforward and non-invasive approach，laser confocal microscopy technique was used to study the effects of different concentrations（ 0，50，75，100，and 75μmol/L ） of Cd on Arabidopsis thaliana root growth. Results showed that root growth was inihibited by Cd treatments（ ≥ 75 μmol/L）. Using fluorescent probes specific for reactive oxygen species（ ROS） and calcium（ Ca），data revealed that Cd was able to increase the concentrations of ROS and Ca accumulated in roots. Furthermore，experiments with probes that lable dead cells and apoptotic cells showed that the viability of root cells decreased with the increase of Cd concentration. Finally，pre-treatment of Ca chelating agent EGTA could decrease ROS concnentration at root cells，while ROS scavenger showed no obvious effect on Ca accumulation. That suggested that upon Cd treatment on the seedlings，Ca accumulation would affect ROS increase in the root cells，that may contribute to the Cd adverse effect on root growth. These studies may provide a feasible approach by using laser confocal miscrocopy on evaluation of Cd effect on plant and also provide experimental data to futher reveal the mechanism how heavy metals affected plant growth and development. |
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