| 决登伟，桑雪莲，舒 波，刘丽琴，王一承，石胜友.玉米WRKY 转录因子非生物胁迫的表达分析[J].广东农业科学,2017,44(1):15-22 |
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玉米WRKY 转录因子非生物胁迫的表达分析 |
| Expression analysis of maize WRKY transcription factor genes under abiotic stress |
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| DOI：10.16768/j.issn.1004-874X.2016.12.003 |
| 中文关键词: 玉米 WRKY 转录因子 干旱 盐胁迫 低温 |
| 英文关键词: maize WRKY TFs drought stress salt stress low temperature stress |
| 基金项目:海南省自然科学基金（20163111）；国家自然科学基金（31572087） |
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| 中文摘要: |
| WRKY 蛋白是一类植物特异的转录因子家族，在植物响应病害及非生物胁迫中起重要作用。
通过生物信息学方法，从玉米基因组中得到3 个WRKY 家族基因序列（ZmWRKY14-like、ZmWRKY25-
like、ZmWRKY62-like），预测表明3 个蛋白都定位于细胞核。荧光定量PCR 分析表明，3 个基因在玉米
的不同器官中都有表达，但具有组织表达特异性。ZmWRKY14-like 和ZmWRKY62-like 在幼果中的表达
量均显著高于在其他组织中的表达量，ZmWRKY25-like 基因在叶、穗丝和幼果中的表达量高于在其他组
织中的表达量。盐胁迫下，ZmWRKY25-like 基因呈上调表达，ZmWRKY62-like 基因呈下调表达；干旱胁
迫下，ZmWRKY62-like 基因出现下调表达；低温胁迫下，ZmWRKY25-like 基因呈上调表达。结果表明，
ZmWRKY25-like 可能参与了植物对盐和低温胁迫的响应，ZmWRKY62-like 基因则可能参与了植物对盐和
干旱胁迫的响应。 |
| 英文摘要: |
| WRKY proteins are large family of plant-specific transcription factors（ TFs），play essential roles
in regulating plant responses to pathogens and abiotic stress. In this study，we identified three WRKY transcription
factor（ TF） genes（ ZmWRKY14-like，ZmWRKY25-like，ZmWRKY62-like） in maize by using bioinformatics
method. Plant-mPLoc predicted that these three proteins were all localized in nucleus. Real-time qPCR assay
showed that ZmWRKY14-like，ZmWRKY25-like，ZmWRKY62-like expressed in all tested maize tissues，but
had differential expression. The expression levels of ZmWRKY14-like and ZmWRKY62-like in young fruit were
significantly higher than those in other tissues，while the expression level of ZmWRKY25-like gene in leaf，
spike and young fruit was higher than that in other tissues. The expression levels of three genes under different
abiotic stress were also analyzed by real-time qPCR. The expression of ZmWRKY25-like was up-regulated，while
ZmWRKY62-like was down-regulated under salt stress. The expression of ZmWRKY62-like was down-regulated
under drought stress. Under low temperature stress，the expression level of ZmWRKY25-like was up-regulated.
These results indicated that ZmWRKY25-like may be involved into plant response to salt and low temperature
stress，and ZmWRKY62-like may be involved into plant response to drought and salt stress. |
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