| 段秀霞1，施文正1，邱伟强1，方 兵1，汪之和1，江 敏2.南美白对虾多酚氧化酶的提取及其性质研究[J].广东农业科学,2017,44(3):136-142 |
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南美白对虾多酚氧化酶的提取及其性质研究 |
| Extraction and characteristics of polyphenoloxidase from white shrimp |
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| DOI：10.16768/j.issn.1004-874X.2017.03.020 |
| 中文关键词: 南美白对虾 多酚氧化酶（PPO） 响应面优化 反应动力学 |
| 英文关键词: white shrimp polyphenol oxidase response surface kinetics |
| 基金项目:国家“十二五”科技支撑计划项目（2015BAD17B01）；上海市虾类现代农业产业技术体系建设项目
（2014-5）；上海市高校知识服务平台项目（ZF1206） |
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| 中文摘要: |
| 以南美白对虾为对象，研究了多酚氧化酶（PPO）分离提取工艺及其生化特性。在单因素试
验基础上，采用响应面试验优化了料液比、提取液pH 值和浸提时间等因素提取南美白对虾PPO 的工艺，
并对PPO 的生化性质进行分析。结果表明：液料比为2∶1、提取液体系pH 值为8.0、浸提时间为3 h 等条
件下，提取的PPO 具有最大酶活力为94.8 A/min·mL，所得回归模型显著（P ＜ 0.0001），方程拟合性好
（R2=0.9762），可用于预测南美白对虾中PPO 的提取；PPO 酶促反应动力学方程符合米氏方程，米氏常数
Km 为30.322 mmol/L，Vmax 为0.281 A/min。PPO 性质研究将为南美白对虾黑变抑制提供理论指导。 |
| 英文摘要: |
| The optimization of extraction and biological characteristics of polyphenol oxidase（ PPO） of white
shrimp（ Penaeus vannamei） were studied. The experimental factors and levels were determined by single factor
experiments. Response surface methodology was used to explore the effects of solid-liquid ratio，pH and extraction
time on PPO activity. The results showed that the optimal extraction conditions were as follows: the solid-liquid ratio
was 2∶1，pH was 8.0 and the extraction time was 3.0 h. Under the optimal extractions，the PPO activity could reach
94.8 A/min·mL. Regressive model of extracted PPO was significant（ P ＜ 0.0001） and better fitting（ R2=0.9762），
it could be used to predict the PPO extraction. The kinetic equation of enzyme reaction was consistent with Michaelis
Menten equation. The Km was 30.322 mmol/L and Vmax was about 0.281 A/min. Study on properties of PPO could
provide theoretical guidance for white shrimp anti-melanosis technology. |
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