| 黄 丹，陈和明，吕复兵.蝴蝶兰丛生芽组织培养研究进展[J].广东农业科学,2017,44(10):19-24 |
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蝴蝶兰丛生芽组织培养研究进展 |
| Advances in clusterd buds tissue culture of Phalaenopsis |
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| DOI：10.16768/j.issn.1004-874X.2017.10.004 |
| 中文关键词: 蝴蝶兰 丛生芽 组织培养 外植体 诱导 褐化防治 |
| 英文关键词: Phalaenopsis clusterd buds tissue culture explant induction proliferation browning prevention |
| 基金项目:广东省科技计划项目（2013B061800038，2017A030303040）；广东省现代农业产业技术体系创新团队
项目（2016LM1096，2017LM1096） |
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| 摘要点击次数: 1939 |
| 全文下载次数: 700 |
| 中文摘要: |
| 蝴蝶兰市场前景广阔，采用组织培养快速繁殖种苗是规模化生产及推广应用的唯一手段，综述
了蝴蝶兰丛生芽组织培养研究进展，旨在为蝴蝶兰种苗规模化生产提供依据。以花梗第2 节或第3 节为外
植体，用0.1% 氯化汞消毒8～15 min 比次氯酸钠效果好；以1/2MS 或花宝一号为基本培养基，使用6-BA
或6-BA 和NAA 相搭配可诱导出丛生芽；以MS 或花宝一号为基本培养基，6-BA 或TDZ 并配合NAA 适宜
的浓度，对丛生芽增殖效果明显；以低浓度的NAA 或IBA，并配以椰子汁或香蕉汁、土豆汁等有机物对壮
苗生根有较好的促进作用；活性炭能显著抑制褐化且对褐化的影响最大，转接周期对褐化的影响次之。 |
| 英文摘要: |
| The Phalaenopsis market has broad prospects，the rapid breeding of seedlings by tissue culture is the
only method for the mass production of Phalaenopsis. The clusterd buds tissue culture of Phalaenopsis was reviewed
in order to provide a basis for the scale production. As the explant in section 2 or 3 of the pedicel，0.1% chlorinated
mercury disinfected 8-15 min was better than sodium hypochlorite. The basic medium of 1/2MS or hyponex No.1，
and 6-BA or 6-BA and NAA could be used to induce clumps. 6-BA or TDZ and the appropriate concentration of
NAA had a significant effect on the proliferation of clusterd buds，and the basic culture medium was either MS or
hyponex No.1. The low concentration of NAA or IBA，and the organic matter such as coconut juice or banana juice，
potato juice and so on had a good effect on the rooting. The activated carbon could inhibit browning and the influence
of browning was first，and the influence of transition cycle on browning was secondary. |
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