| 黄燕琼1，邓 艳1，张 森2，何淑华1，戴 金1，柏建山1.黄鳝和泥鳅感染颚口线虫的分类鉴定研究[J].广东农业科学,2017,44(10):102-108 |
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黄鳝和泥鳅感染颚口线虫的分类鉴定研究 |
| Classification and identify of infected Gnathostoma sp. from Monopterus albus and loaches |
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| DOI：10.16768/j.issn.1004-874X.2017.10.017 |
| 中文关键词: 颚口线虫 形态学鉴定 序列分析 系统进化树 |
| 英文关键词: Gnathostoma morphological identification sequence analysis phylogenetic tree |
| 基金项目:广东检验检疫局科技专项（2016GDK13） |
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| 中文摘要: |
| 对从白云机场口岸入境的黄鳝（19 批次）和国内部分地区（广东广州、重庆、四川内江、湖北
荆州、福建漳州和吉林长春）市售的黄鳝和泥鳅进行剖检。将内脏和肌肉进行蛋白酶消化后，取滤液沉淀，
体视显微镜下挑出虫体进行形态学鉴定，确定所分离出的虫体为颚口线虫三期幼虫。印尼黄鳝中检出颚口
线虫三期幼虫，批次阳性率达到91.7%；菲律宾黄鳝中未检出颚口线虫。国内只在漳州的泥鳅中发现了18
条虫体，在黄鳝中发现了1 条虫体，其他地区均无发现虫体。提取基因组DNA 后，PCR 扩增核糖体DNA 第
二内转录间隔区（internal transcribed spacer region 2，ITS2）基因，对产物测序后结果进行多重序列比对，构
建系统进化树。确定印尼黄鳝中分离的颚口线虫为棘颚口线虫（Gnathostoma spinigerumn）和刚刺颚口线虫
（G. hispidum）；福建漳州地区分离出的19 条颚口线虫，其中17 条为日本颚口线虫（G. nipponicum）、2 条
为棘颚口线虫（G. spinigerumn）。 |
| 英文摘要: |
| To identify the third stage larvae of Gnathostoma in Monopterus albus and loaches，19 batches of M.
albus imported from the port of Baiyun Airport in Guangzhou，12 batches of M. albus and loaches from Guangzhou，
Chongqing，Sichuan Neijiang，Hubei Jingzhou，Fujian Zhangzhou and Jilin Changchun were inspected. After
digestion of the viscera and muscles，the filtrate was precipitated and the larvaes were picked out under the stereo
microscope. Morphological identification was carried out to determine that the isolated larvaes were the third larvae
of Gnathostoma. The infection rate in M. albus from Indonesia was 91.7%，but no Gnathostoma was found in M.
albus from Philippines. At the same time，18 Gnathostoma from loaches and 1 Gnathostoma from M. albus were
isolated from Zhangzhou city，but no Gnathostoma was found in other cities at home. Genomic DNA of Gnathostoma
was extracted to amplify internal transcribed spacer region 2（ITS-2） by PCR，the product of which was analyzed
by electrophoresis and sequencing. The sequences were aligned with corresponding sequences in GenBank，
and phylogenetic tree was constructed. Morphological dentification and sequence alignment analysis results showed
that the larvae from Indonesia was G. spinigerumn and G. hispidum，the larvae from Zhangzhou city was G. nipponicum
and G. spinigerumn. |
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