| 刘蒙娜，刘 媛，马敏怡，刘书成，吉宏武，高 静，郝记明，毛伟杰.3 种巯基化合物对冷藏条件下凡纳滨对虾的防黑变和保鲜效果[J].广东农业科学,2018,45(1):94-101 |
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3 种巯基化合物对冷藏条件下凡纳滨对虾的防黑变和保鲜效果 |
| Inhibitory melanosis and preservation of three sulfydryl compounds on Litopenaeus vannamei duringrefrigerated storage |
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| DOI：10.16768/j.issn.1004-874X.2018.01.016 |
| 中文关键词: 凡纳滨对虾 抑制剂 巯基化合物 抑制黑变 保鲜 冷藏 |
| 英文关键词: Litopenaeus vannamei inhibitor sulfhydryl compounds melanosis inhibition preservation refrigerated storage |
| 基金项目:国家自然科学基金（31771997，31371801）；广东海洋大学创新强校重大科研培育项目（GDOU
2017052603）；国家现代农业产业技术体系专项基金（CARS-47）；广东普通高等学校水产品高值化加工与利用创新
团队项目（GDOU2016030503） |
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| 中文摘要: |
| 研究前期筛选了3 种对凡纳滨对虾多酚氧化酶具有较好抑制效果的巯基化合物（L- 半胱氨
酸、还原性谷胱甘肽和N- 乙酰-L- 半胱氨酸），为进一步探讨它们对凡纳滨对虾的防黑变效果，以无菌
水、亚硫酸钠和4- 己基间苯二酚（4-HR）处理为对照，利用黑变评分、相对灰度值、感官评价、菌落总
数、挥发性盐基氮和pH 值等指标评价其对4℃贮藏的凡纳滨对虾的防黑变和保鲜效果。结果表明：与无菌
水处理组相比，3 种巯基化合物对凡纳滨对虾均具有较好的防黑变效果，当贮藏到10 d 时，L- 半胱氨酸、
还原性谷胱甘肽和N- 乙酰-L- 半胱氨酸处理虾体的黑变评分分别为3（±0.81）、3（±0.81）和5.33
（±0.73）分，均未超过最大可接受值6 分，而且与亚硫酸钠的防黑变效果相当，但弱于4-HR；但3 种巯
基化合物对凡纳滨对虾不具有明显的保鲜效果。因此，3 种巯基化合物可代替亚硫酸钠作为有效的虾类黑
变抑制剂，但是如果要同时对虾进行保鲜，必须与其他抑菌物质复配。 |
| 英文摘要: |
| Litopenaeus vannamei is one of the very popular seafoods with higher nutritional value. However，
melanosis of the shrimp occurs easily because polyphenol oxidase（ PPO） catalyses a series of biochemical reactions
during storage and processing after capture. Although melanosis does not affect the nutritional value of shrimp，it
seriously affects shrimp sensory quality and commercial value，then causes severe economic loses. At present，sulfite
and 4-hexylresorcinol（ 4-HR） are widely used to prevent melanosis of the shrimps commercially. However，some
researches show that they have potential hazards to human health and have been prohibited to use as food additives in many countries. Therefore，it is very important to develop green，safe and effective melanosis inhibitors of shrimps.
In the previous studies，three sulfydryl compounds（ L-cysteine，glutathione and N-acetyl-L-cysteine） had better
inhibitory effects on PPO from L. vannamei. In this study，regarding sterile water，sodium sulfite and 4-HR treatment
as controls，melanosis inhibition of three sulfhydryl compounds on L. vannamei was explored during refrigerated storage.
The results show as follows：Compared with the sterile water treatment，three sulfydryl compounds have better inhibitory
melanosis effect on L. vannamei. Three sulfydryl compounds have the same inhibitory melanosis effect on L. vannamei
with sodium sulfite，but less than that of 4-HR. Three sulfydryl compounds do not have obvious preservation effect on L.
vannamei. Therefore，three sulfhydryl compounds can replace sodium sulfite as effective melanosis inhibitors of shrimps.
They must be mixed with other antibacterial substances if shrimps need to be preserved meanwhile. |
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