| 许卫华1，2，赵殿强1，黄 芬1，李虹仪1，张 茂1，孙艳发1.槐猪FTO 基因遗传多态性及其与肉质性状相关性研究[J].广东农业科学,2018,45(1):108-113 |
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槐猪FTO 基因遗传多态性及其与肉质性状相关性研究 |
| Diversity analysis of FTO gene and their association with meat quality traits in Huai Pig |
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| DOI：10.16768/j.issn.1004-874X.2018.01.018 |
| 中文关键词: 槐猪 FTO 基因 肉质性状 PCR-RFLP |
| 英文关键词: Huai pig FTO gene meat quality traits PCR-RFLP |
| 基金项目:福建省自然科学基金(2015J01620)；福建省中青年教师教育科研项目（JA14306）；龙岩学院引进人才
科研启动经费（LB2014025） |
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| 摘要点击次数: 1937 |
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| 中文摘要: |
| 以上杭槐猪为试验材料，对FTO 基因5′上游区域SNP g.-167 T>G、第一内含子SNP c.46-
139A>T 和第三外显子同义突变SNP c.594C>G 遗传多态性及其与肌内脂肪含量、背膘厚度、眼肌面积等肉
质性状的相关性进行研究，3 个突变分别产生EcoR Ⅰ、Xap Ⅰ和Bsh1236I 等3 个限制性内切酶酶切位点。
结果表明：被测猪群在3 个位点均存在一定的遗传多态性，PIC 值分别为0.276、0.343 和0.235，各位点等位
基因型频率均处于Hardy-Weinberg 平衡状态；g.-167 T>G 位点，等位基因G 为优势等位基因，其基因频率
为0.791，然而，TT 基因型肌内脂肪含量显著高于GG 基因型；c.46-139A>T 位点，等位基因T 为优势等位
基因，其基因频率为0.674，相对于AA 基因型，TT 基因型显著降低了背膘厚度；c.594C>G 位点，等位基因
G 为优势等位基因，其基因频率为0.837，各基因型个体之间在3 个被测性状中差异均不显著。因此，FTO
基因g.-167 T>G 和c.46-139A>T 可作为槐猪肉质性状选育的潜在分子遗传标记。 |
| 英文摘要: |
| PCR-RFLPs with EcoR Ⅰ，Xap Ⅰ and Bsh1236 I restriction enzymes were used to analyze
genetic diversities of three SNPs at the 5′-upstream region（g.-167 T>G），the first intron（c.46-139A>T） and
the third exon（c.594C>G） of the FTO gene，and potential associations between genotypes and three meat quality
traits including intramuscular fat（IMF），backfat thickness（BFT） and loineye area（LEA） were investigated.
The results showed that three SNPs presented certain degrees of diversities with the PIC value being 0.276，0.343
and 0.235，respectively；and the frequencies of observed genotypes conformed to Hardy Weinberg equilibrium
（P>0.05）；for g.-167 T>G SNP，G allele was dominant with the frequency being 0.790，whereas CC genotype was
statistically raised IMF content；for c.46-139A>T SNP，T allele was dominant with the frequency being 0.674，and
TT genotype was statistically decreased BFT comparing toAA genotype；for c.594C>G SNP，G allele was dominant
with the frequency being 0.837，and the SNP were not statistically associated with tested traits. In summary，two SNPs of g.-167 T>G and c.46-139A>T could be potential molecular genetic markers for the meat quality traits in the
population of Huai pigs. |
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