| 陈美伶，姜玉松.生姜转录因子基因ZoNAC17 的克隆与表达分析[J].广东农业科学,2018,45(6):30-35 |
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生姜转录因子基因ZoNAC17 的克隆与表达分析 |
| Cloning and expression analysis of transcription factor gene ZoNAC17 in ginger |
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| DOI：10.16768/j.issn.1004-874X.2018.06.006 |
| 中文关键词: 生姜 NAC 转录因子 基因克隆 数字表达谱 表达分析 |
| 英文关键词: Ginger NAC transcription factors gene cloning digital expression profile expression analysis |
| 基金项目:国家自然科学基金（31501273）；重庆市前沿与应用基础研究（一般）项目（cstc2015jcyjA8003） |
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| 中文摘要: |
| NAC 类转录因子在植物逆境胁迫应答过程中具有极其重要的作用。为进一步探索NAC 转录因
子的结构与功能，基于生姜转录组数据库，利用RT-PCR 法克隆生姜转录因子基因ZoNAC17，采用数字表
达谱分析生姜不同组织的ZoNAC17 表达图谱。序列分析结果显示，生姜ZoNAC17 基因的ORF 长度为1 815
bp，编码604 个氨基酸残基，分子量为67.52 kD；转录因子ZoNAC17 等电点为4.59，不存在信号肽，为跨膜
蛋白，亚细胞定位于细胞核。系统进化树分析表明，生姜ZoNAC17 与小果野蕉MaNAC17 基因亲缘关系最
近。数字表达谱结果表明，ZoNAC17 在生姜不同组织中均有较强表达，在成熟组织中的表达量为地上茎＞
叶片＞根茎。在生姜根茎的发育过程中，ZoNAC17 表达量先增加，后逐渐减少，且在生长约3 个月的发育期
表达量最高。 |
| 英文摘要: |
| NAC transcription factor plays an important role in the response of plant stress. To further explore
the structure and function of NAC transcription factors，based on the ginger transcriptional database，using RTPCR
method to clone the transcription factor gene ZoNAC17 of ginger，and analyze the ZoNAC17 expression pattern
of different tissues of ginger by digital expression profile. The sequence analysis results showed that the length of
ZoNAC17 gene’s ORF in ginger is 1 815 bp，encoding 604 amino acids residues，molecular weight is 67.52 kD.
ZoNAC17 transcription factor is a transmembrane protein without signal peptide，the isoelectric point is 4.59，and
the subcellular location is located in the nucleus. The phylogenetic analysis indicated that ZoNAC17 gene in ginger
had the closest genetic relationship with MaNAC17 gene of Musa acuminata. The results of digital expression profile
displayed that ZoNAC17 gene is strongly expressed in different tissues of ginger，and the expression level of ZoNAC17
in mature tissues is different，which is higher in stem than leaf，and is more in leaf than rhizome. During the
development of rhizomes of ginger，the expression level of ZoNAC17 increased first and then decreased gradually，
and the highest expression level is in the development period of about 3 months. |
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