| 柳婷婷1，2，刘南南1，王桂清1.康宁霉素对臭椿苗期致病菌的离体抑菌活性[J].广东农业科学,2018,45(7):81-86 |
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康宁霉素对臭椿苗期致病菌的离体抑菌活性 |
| Fungistasis of trichokonins on pathogensin Ailanthus seedling in vitro |
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| DOI：10.16768/j.issn.1004-874X.2018.07.013 |
| 中文关键词: 康宁霉素 臭椿 苗期致病菌 杀菌剂 毒力测定 抑菌作用 |
| 英文关键词: trichokonins Ailanthus pathogens in seedling fungicide toxicity determination fungistasis |
| 基金项目:山东省自然科学基金联合专项（ZR2012CL17）；聊城市科技发展计划项目（2014GJH10） |
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| 中文摘要: |
| 以引起臭椿苗期病害的4种致病菌腐皮镰刀菌（Fusarium soiani）、木贼镰刀菌（F. equiseti）、棒状拟盘多毛孢菌（Pestalopsis clavispora）和极细链格孢菌（Alernaria tenuissima）为靶标，以康宁霉素水溶剂为研究对象，以苯醚甲环唑、多菌灵、苦参碱和霜霉立克为阳性对照，采用生长速率法和孢子萌发法，研究康宁霉素对致病菌的菌丝生长和孢子萌发的影响。结果表明，康宁霉素对4种致病菌菌丝生长和孢子萌发均有抑制作用，药剂浓度越高，抑制效果越明显，且对致病菌孢子萌发的抑制效果优于对菌丝生长的抑制效果；其中康宁霉素对棒状拟盘多毛孢菌的菌丝生长和孢子萌发EC50最小，分别为32.97 mg/L和47.99 mg/L；虽然TKS的抑菌效果低于化学药剂多菌灵和生物制剂苦参碱，但优于霜霉立克，说明康宁霉素可用于防治由此4种病原菌引起的臭椿苗期病害。该研究为扩大康宁霉素的应用范围和臭椿苗期病害的生物防治提供了理论依据。 |
| 英文摘要: |
| In this study, four kinds of pathogens that caused diseases in Ailanthus seedling（Fusarium soiani, F.equiseti, Pestalopsis clavispora and Alernaria tenuissima） were selected as the targets to research the fungistasis of trichokonins. The inhibitory effects of trichokonins of hyphae growth and spores germination of pathogens were determinated using hyphae growth rate and spores germination methods, with Difenoconazole, Carbendazim, Matrine and Downy mildew like as the positive control. The results showed that trichokonins had significant antibacterial activities of tested pathogens. The higher the concentration，the stronger the inhibition. And meanwhile the inhibited effect on spores germination was better than that on hyphae growth. The effect on hyphae growth and spores germination of P. clavispora was best, with EC50=32.97 mg/L, and 47.99 mg/L. The fungistasis of trichokonins was poorer than that of Carbendazim and Matrine, while stronger than that of Downy mildew like, thus trichokonins can control the diseases caused by these four kinds of pathogens in Ailanthus seedling. This study provided a theoretical basis for expanding the application range of trichokonins and the biological control of the diseases in Ailanthus seedling. |
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