| 韩向峰1，李志芳1，曹 琴2，张海平3，程小爱4.农大5号钙果叶片芽诱导及其植株再生研究[J].广东农业科学,2018,45(8):37-42 |
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农大5号钙果叶片芽诱导及其植株再生研究 |
| Study on bud induction and regeneration fromleaves of Nongda No.5 in Chinese dwarf cherry |
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| DOI：10.16768/j.issn.1004-874X.2018.08.006 |
| 中文关键词: 欧李 农大5号 叶片 不定芽 再生 |
| 英文关键词: Chinese dwarf cherry Nongda No.5 leaves adventitiousbuds regeneration |
| 基金项目: |
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| 摘要点击次数: 1720 |
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| 中文摘要: |
| 采集定植于温室中苗木上的叶片和组培瓶中农大5号钙果分化苗、壮苗叶片，进行离体叶片再生芽诱导及其植株再生体系研究。结果表明，温室叶片诱导不定芽的最佳培养基为1/2MS+IAA 0.1 mg/L+NAA 0.05 mg/L+6-BA 0.8 mg/L，培养基中全部元素减半；组培分化苗叶片和壮苗叶片诱导不定芽的最佳培养基为1/2MS+IAA 0.05 mg/L+NAA 0.1 mg/L+6-BA 0.8 mg/L，培养基中大量元素减半；辅助因子以培养基中添加AgNO3 0.2 mg/L、全光照、叶片反放和带叶柄的叶基部等措施配合取得了最高的芽诱导率。 |
| 英文摘要: |
| In this study, leaves of Chinese dwarf cherry variety Nongda No.5 were collected from greenhouse
cultivated seedlings and tissue cultured seedlings respectively. Bud induction and plant regeneration were studied.
Theoptimum medium for adventitious buds induction of leaves from greenhouse was 1/2MS+IAA 0.1 mg/L+NAA
0.05 mg/L+6-BA 0.8 mg/L, in which all the nutrients were halved.The best medium for adventitious buds induction
of leaves from tissue cultured seedlings was1/2MS+IAA 0.05 mg/L+NAA 0.1 mg/L+6-BA 0.8 mg/L, in which
macro-elements were halved. Moreover, auxiliary factor studies showed that the highest bud induction rate was
achieved with the combination of adding 0.2 mg/L AgNO3 into the medium, full light exposure, turning leaves
upside down and using leaves with petioles. |
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