| 林奕云,林耀盛,郑家概,等.响应面法优化微波结合酶解制备米糠多肽工艺对黄曲霉毒素B1降解效果的研究[J].广东农业科学,2019,(6):- |
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响应面法优化微波结合酶解制备米糠多肽工艺对黄曲霉毒素B1降解效果的研究 |
| Study On Modeling and Optimization Microwave- Assisted Enzyme Treatment during the preparation of rice bran polypeptide on Degradation of Aflatoxin B1 (AFB1) |
| 投稿时间：2019-04-15 修订日期：2019-04-15 |
| DOI： |
| 中文关键词: 关键词：米糠多肽 微波处理 酶法 黄曲霉毒素B1 响应面脱除 |
| 英文关键词: Key words: polypeptide of rice bran bran Microwave treatment enzymatic pretreatment AFB1 response surface method for detoxification |
| 基金项目:基金项目：公益性行业（农业）科技专项(201203037-01-01)；国家重点研发计划项目（2017YFD0401105）；广东省省级科技计划项目（2015A030310466） |
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| 中文摘要: |
| 摘要：【目的】研究微波结合酶解法组合技术对米糠多肽黄曲霉毒素B1（aflatoxin B1，AFB1）的影响，为染毒米糠的脱毒提供新途径。【方法】在单因素试验的基础上，将料液比（X1）、微波时间（X2）、碱性蛋白酶酶解时间（X3）、酶解pH（X4）因素进行组合，应用Design Expert软件和Box-Behnken中心组合试验方法对AFB1降解条件进行了优化，测定米糠多肽AFB1的脱除量。【结果】初始米糠中AFB1浓度为100.14 μg/kg，运用响应面分析确定了其最佳工艺条件：微波功率750W、微波时间9.56min；碱性蛋白酶酶解温度50℃、酶解pH 10.74、酶解时间1.57h、加酶量0.4%；料液比1∶9.96，在此条件下，米糠中AFB1的降解率为98.68%，所制备的米糠多肽残留浓度为1.85 ug/kg，符合国家标准（≤10ug/kg）。【结论】与单因素处理相比，微波结合酶法制备工艺能发挥协同作用，有效降低米糠AFB1含量。 |
| 英文摘要: |
| Abstract: 【Objective】To provide new pathways for detoxification of rice bran, the effect of microwave combined with enzymatic hydrolysis on aflatoxin B1 (AFB1) were studied in rice bran. 【Method】 On the basis of single factor experiment, the factors of material-liquid ratio (X1), microwave time (X2), alkaline protease hydrolysis time (X3) and enzymatic hydrolysis pH (X4) were combined. Design Expert software and Box-Behnken central combination test method were used to optimize the degradation conditions of AFB1 and determine the removal amount of rice bran polypeptide AFB1. 【Result】 The initial concentration of AFB1 in rice bran was 100.14 ug/kg, and the optimum technological conditions were determined by response surface analysis: microwave power 750 W, microwave time 9.56 min; alkaline protease hydrolysis temperature 50 ℃, enzymatic hydrolysis pH 10.74, enzymatic hydrolysis time 1.57 h, enzymatic dosage 0.4%; material-liquid ratio 1:9.96. Under these conditions, the degradation rate of AFB1 in rice bran was 98.68%, and the residual concentration of rice bran polypeptide was obtained. 1.85 ug/kg, in line with the national standard (<10ug/kg). 【Conclusion】Compared with single factor treatment, microwave combined with enzymatic method can play a synergistic role and effectively reduce the content of rice bran AFB1. |
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