| 古广武,董辰韵,钟伟强,等.聚硅氧烷-聚丙烯酸酯季铵盐嵌段共聚物对水稻纹枯病菌细胞膜的影响[J].广东农业科学,2019,(6):- |
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聚硅氧烷-聚丙烯酸酯季铵盐嵌段共聚物对水稻纹枯病菌细胞膜的影响 |
| Effect of polysiloxane-polyacrylate quaternary ammonium salt block copolymer on cell membrane of Rhizoctonia solani |
| 投稿时间：2019-04-30 修订日期：2019-04-30 |
| DOI： |
| 中文关键词: 聚硅氧烷-聚丙烯酸酯季铵盐嵌段共聚物 R. solani 细胞膜 脂质过氧化 |
| 英文关键词: Polysiloxane-polyacrylate quaternary ammonium block copolymer R. solani Cell membrane Lipid peroxidation |
| 基金项目:广州市科技计划项目(201704020084、201803020015),国家自然科学(31772202) |
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| 中文摘要: |
| 【目的】研究聚硅氧烷-聚丙烯酸酯季铵盐嵌段共聚物(Six-Q5)对于水稻纹枯病菌细胞膜的影响,为水稻纹枯病的防治提供新思路。【方法】通过PI染色后在微分干涉显微镜下观察菌丝形态表征Six-Q5对水稻纹枯病菌细胞膜完整性的影响；通过考马斯亮蓝法、蒽酮-硫酸法以及电导率法表征Six-Q5对水稻纹枯病菌细胞膜渗透性的影响；利用丙二醛(MDA)试剂盒测定丙二醛的含量变化,来表征Six-Q5对水稻纹枯病菌细胞膜脂质过氧化的影响。【结果】经过Six-Q5处理的菌丝显微镜下可观测到荧光亮斑；菌丝培养液中的蛋白质、碳水化合物和电解质均有明显区别于对照组的变化；水稻纹枯病菌菌丝以及菌丝培养液中的MDA含量均随着药物浓度的增加而增加；且随着PDMS嵌段长度的不同,其对于R. solani菌丝细胞膜的影响也有所差别。【结论】实验结果表明,Six-Q5破坏了水稻纹枯病菌细胞膜的完整性,改变了细胞膜的渗透性以及细胞膜的结构功能完整性；且随着Six-Q5的嵌段长度增加,其影响更加显著。细胞膜是Six-Q5抑制水稻纹枯病菌一个作用靶点。 |
| 英文摘要: |
| 【Objective】We studied the effect of polysiloxane-polyacrylate quaternary ammonium salt block copolymer (Six-Q5)on cell membrane of rice sheath blight, thus we can provide a new idea for the control of rice sheath blight.【Method】The effects of Six-Q5 on cell membrane integrity of Rhizoctonia solani were studied by PI staining and differential interference microscopy, and the effects of Six-Q5 on cell membrane permeability of Rhizoctonia solani were characterized by Coomassie brilliant blue method, anthrone-sulfuric acid method and conductivity method. Malondialdehyde (MDA) assay kit was used to characterize the effect of Six-Q5 on lipid peroxidation of cell membrane of Rhizoctonia solani.【Results】Under the fluorescence microscope, the morphology of mycelia treated with Six-Q5 were observed with fluorescent bright spots. We found that the protein, carbohydrate and electrolyte contents in the mycelium culture medium of the treatment were significantly different from those of the control. The MDA content of mycelia and culture medium in the treatment increased with the increase of drug concentration. With the difference of PDMS block length, its influence on the R. solani mycelia membrane was also different.【Conclusion】The results show that Six-Q5 destroyed the integrity of cell membrane of rice sheath blight fungus and changed the permeability of cell membrane and the structural and functional integrity of cell membrane. Moreover, with the increases of the block length of Six-Q<sub>5</sub>, the effect is more pronounced. Cell membrane is a target of Six-Q<sub>5</sub> for inhibiting rice sheath blight. |
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