| 蔡时可,梅瑜,顾艳,等.走马胎的离体培养与快速繁殖[J].广东农业科学,2019,(9-10):- |
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走马胎的离体培养与快速繁殖 |
| In vitro culture and rapid propagation of Ardisia gigantifolia |
| 投稿时间：2019-06-25 修订日期：2019-06-25 |
| DOI： |
| 中文关键词: 走马胎 组织培养 快速繁殖 离体培养 |
| 英文关键词: Ardisia gigantifolia Tissue culture Rapid propagation In vitro culture |
| 基金项目:广东省农业科学院2018年农业科技特派员精准扶贫乡村产业振兴支撑项目(T2018021)；农业部华南都市农业重点实验室开放(016) |
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| 摘要点击次数: 436 |
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| 中文摘要: |
| 【目的】走马胎是我国传统药用植物,长期依赖自然采挖,以致野生资源枯竭,急需发展驯化种植。本研究采用走马胎的茎作为外植体,建立了组织培养快速繁殖方法【方法】采用70%酒精和0.1%的升汞溶液为消毒液设置不同处理时间对外植体进行消毒；得到无菌外植体后接种在含有6-BA、KT和IBA的改良MS培养基进行丛芽增殖；采用1/2改良MS培养基作为基础培养基,添加 NAA 和IBA不定芽进行生根诱导；再生植株经过炼苗后,移栽。【结果】外植体消毒采用70%酒精处理30秒结合0.1%的升汞溶液消毒处理7-9分钟最为合适；诱导不定芽的增殖培养基配方为改良MS培养基 + 6-BA 1.0 mg/L +KT1mg/L +IBA 0.05 mg/L；生根培养基配方为1/2改良MS培养基 + NAA 0.2 mg/L +IBA 0.3 mg/L。【结论】通过对快速繁殖体系中外植体的消毒方法,增殖和生根培养基的优化,建立了走马胎的离体培养与快速繁殖方法,为人工种植提供了种苗基础。 |
| 英文摘要: |
| 【Objective】Ardisia gigantifolia is an important traditional Chinese herbal medicine, which relies on harvesting from nature for a long time, so that resources are almost exhausted and the market is shortages. In this study, stems of Ardisia gigantifolia were took as the explants to establish a rapid propagation method by tissue culture in vitro.【Method】The explants were sterilized with 70% alcohol and 0.1% Mercury chloride solution for different treatment time; cluster buds were inoculated in the modified MS medium containing 6-BA, KT and IBA with the aseptic explant proliferates; the 1/2 modified MS medium with NAA and IBA were used to induce rooting for the adventitious buds; after seedling refining, the regenerated plants were transplanted.【Result】The result showed that disinfecting the stems via 70% alcohol for 30 seconds and 0.1% Mercuric chloride solution together with 7-9 mins was the best methods; modified MS medium with 1.0 mg/L 6-BA KT and 0.05 mg/L IBA was the optimal combinations for inducing and reproduction. The 1/2 MS medium with 0.2 mg/L NAA and 0.3 mg/L IBA was suite for rooting.【Conclusion】By optimizing the sterilization method, proliferation and rooting medium of explants in rapid propagation system, the method of in vitro culture and rapid propagation of Ardisia gigantifolia was established, which provided seedling enough for artificial planting and alleviate the market demand effectively. |
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