| 何艺平,陈杰,高鑫凤,等.白麻羽性状与SLC45A多态性关系[J].广东农业科学,2019,(10-11):- |
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白麻羽性状与SLC45A多态性关系 |
| Relationship between white-partridge feathers trait and SLC45A polymorphism |
| 投稿时间：2019-08-14 修订日期：2019-09-18 |
| DOI： |
| 中文关键词: 文昌鸡 白麻羽 SLC45A2 银色羽基因座 |
| 英文关键词: Wenchang Chicken White-partridge feather SLC45A2 SLV |
| 基金项目:江西省教育厅科技项目(GJJ181080)；南昌师范学院启动基金；南昌师范学院“11531”工程建设项目。 |
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| 中文摘要: |
| 【目的】本文研究了文昌鸡白麻羽性状的遗传规律并鉴定了与白麻羽性状相关的基因变异，其目的是为文昌鸡白麻羽群体的利用以及白麻羽性状的分子标记辅助选择提供参考依据。【方法】首先利用正反交实验探索白麻羽性状的遗传规律；然后根据白麻羽性状的特点结合前人研究结果，选取SLC45A2基因为候选基因，利用重测序的方法筛选外显子区域内的变异；最后重点验证外显子区域的错义突变和无义突变与白麻羽性状的关系。【结果】黄麻羽个体与白麻羽个体的正反交试验结果表明：白麻羽性状相对于黄麻羽呈伴性隐性遗传。实验以白麻羽和黄麻个体为DNA模板，扩增了SLC45A2上的7个外显子区域。结果共筛选到5个SNP，其中3个位于外显子上，2个为错义突变（c.a74g和c.g1154c），1个为同义突变（c.c561t）。以文昌鸡白麻羽群体的文昌鸡和非白麻羽性状的文昌鸡、霸王山鸡、清远麻鸡、杏花鸡、红色原鸡为材料，验证筛选到上述错义突变，结果显示c.g1154c位点与白麻羽性状完全连锁。【结论】通过实验结果可知，白麻羽性状呈现伴性隐性遗传，c.g1154c可能是白麻羽性状的致因突变。 |
| 英文摘要: |
| 【Objective】In this study, we studied the inheritance of the trait of white-partridge feathers in Wenchang chicken and identified the related genetic variation. The results will be valuable for molecular marker-assisted selection of white-partridge and hybrid hybrid utilization of the white-partridge population. 【Method】Firstly, the inheritance of white-partridge feathers was studied by reciprocal test. Then, SLC45A2 was selected as a candidate gene based on the genetic characteristics of white-partridge feather and its phenotypic traits. The variation in the exon domain of SLC45A2 gene was analyzed using re-sequencing method. Lastly, the relationship between polymorphism in the exon domain and the trait of white-partridge was verified. 【Result】The result of reciprocal test showed that the trait of white-partridge was recessive inheritance compared with yellow-partridge trait. The amplification result indicated that there were five SNPs in SLC45A2 gene, three of them were located in exon domain, two of them were missense mutation (c.a74g and c.g1154c), and one of them was synonymous mutation (c.c561t). Finally, the result showed that C.G1154C site of SLC45A2 gene was completely linked with the white-partridge trait based on missense mutation study in Wenchang Chicken, Bawang Chicken, Qingyuan Partridge Chicken, Xinghua Chicken and Gallus gallus. 【Conclusion】In conclusion, trait of white-partridge feathers showed recessive inheritance and c.g1154c site might be the causation. |
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