| 吉薇,刘扬,龙慧静.扁舵鲣鱼低聚肽化学组成及其对人体肝癌细胞的抑制作用[J].广东农业科学,2019,(11-12):- |
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扁舵鲣鱼低聚肽化学组成及其对人体肝癌细胞的抑制作用 |
| Chemical Composition of Oligopeptide Derived from Auxis thazard Protein and Its Inhibiting Activity on Human Liver Cancer Cells |
| 投稿时间：2019-08-25 修订日期：2019-09-23 |
| DOI： |
| 中文关键词: 扁舵鲣鱼 低聚肽 化学组成 抑制活性 肝癌细胞 细胞培养 |
| 英文关键词: Auxis thazard Oligopepteds chemical composition inhibiting activities liver tumor cells cell culture |
| 基金项目:国家自然科学基金(31801614)；广东省普通高校青年创新人才项目(2017KQNCX141)；农业部水产品加工重点实验室开放(NYJG201703)；广东省水产品加工与安全重点实验室开放(GDPKLAPPS1703) |
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| 中文摘要: |
| 【目的】为扁舵鲣鱼低聚肽开发抗肝癌辅疗食品提供技术依据。【方法】本研究以扁舵鲣鱼为原料，采用复合酶水解、两级膜耦联分离和喷雾干燥等技术制备扁舵鲣鱼低聚肽，测定其分子量的分布、氨基酸组成和矿物质成分，采用细胞培养实验，测定其对人体HepG2、BEL-7402与SMMC-7721肝癌细胞抑制活性。 【结果】扁舵鲣鱼低聚肽蛋白质含量为88.90%，低聚肽含量为71.96 g/100g，肽分子量主要在1000 u以下，占总肽的89.79%；谷氨酸、赖氨酸、天冬氨酸、亮氨酸、精氨酸等5种氨基酸丰富，占总氨基酸的52.41%；硒与锌含量高，分别为2.84、10.10 mg/100g。细胞培养实验结果显示：与阴性对照组相比，扁舵鲣鱼低聚肽对HepG2、BEL-7402与SMMC-7721肝癌细胞都具有抑制作用，且具有剂效关系，但对对抗肿瘤药物顺铂的抗癌作用无增敏效应。高剂量低聚肽组对HepG2、BEL-7402与SMMC-7721肝癌细胞均具有显著抑制作用（p<0.01），中剂量组也具有显著的作用（p<0.05）。【结论】扁舵鲣鱼低聚肽是一种良好的抗肝瘤辅疗食品基料。 |
| 英文摘要: |
| 【Objective】To supply technology basis for the oligopeptide derived from Auxis thazard protein developing anticancer food for liver tumors 【Method】 The oligopeptide was prepared from Auxis thazard protein by using complex enzyme hydrolysis, membrane separation and spray drying, and its molecular weight distribution, amino acid composition and mineral composition were analyzed. Its inhibiting activities on 3 kinds of human liver cancer cells (HepG2、BEL-7402 and SMMC-7721) in vitro were determined by cell culture experiments. 【Result】Chemical components analysis results showed that protein content of the oligopeptide derived from Auxis thazard was 88.9%，the total oligopeptide was 71.96 g/100g and its molecular weight was mainly below 1000u, accounting for 89.79% of the total dry weight. Glutamic acid, lysine, aspartic acid, leucine and arginine were higher in the amino acid content, accounting for 52.41% of the total amino acids. It was rich in selenium and zinc, which was 2.84 mg/100g and 10.10 mg/100g, respectively. Cell culture results showed that Compared to the negative control group, the oligopeptides groups all exhibited inhibiting activity on HepG2, BEL-7402 and SMMC-7721 cells and had dose-effect dependence，but it did not effectively increased cisplatin inhibiting activity on HepG2, BEL-7402 and SMMC-7721 cells. High dose group showed significant inhibiting activities on HepG2, BEL-7402 and SMMC-7721 cells (p<0.01), middle dose group also had significant inhibiting effect on HepG2, BEL-7402 and SMMC-7721 cells (p<0.05). 【Conclusion】So the oligopeptide derived from Auxis thazard was a good anticancer food base for liver tumors. However, Its anti-cancer activity in vivo and inhibiting mechanism on liver cancer still need further studying. |
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