| 张云,赵艳菲,王雅平,等.延薯4号马铃薯对氮素的生理响应及转录组分析[J].广东农业科学,2019,(11-12):- |
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延薯4号马铃薯对氮素的生理响应及转录组分析 |
| Physiological response and transcriptome analysis of nitrogen in Yanshu No.4 potato |
| 投稿时间：2019-08-31 修订日期：2019-09-24 |
| DOI： |
| 中文关键词: 马铃薯 转录组测序 生理响应 氮代谢 差异基因 |
| 英文关键词: Potato Transcriptome sequencing Physiological response Nitrogen metabolism Differences in gene |
| 基金项目:吉林省马铃薯新品种‘富农1号’脱毒种薯繁育及产业化开发 |
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| 摘要点击次数: 198 |
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| 中文摘要: |
| 【目的】为了研究不同氮浓度处理对马铃薯的生理生化响应以及氮代谢相关基因的挖掘。【方法】以“延薯4号”马铃薯为试材,设置施氮与未施氮处理,采用盆栽种植的方式研究了马铃薯氮效率、生理生化差异以及生长发育关键时期,并且对马铃薯现蕾期的叶和根进行转录组测序分析,获得氮代谢差异表达基因。【结果】结果表明：施氮处理显著增加了马铃薯氮效率,可溶性糖含量,可溶性蛋白含量,根系活力,硝酸还原酶活性,谷氨酰胺合成酶活性,同时现蕾期为马铃薯生长发育关键时期；施氮的叶和根中共有12996个DEGs,6440个上调,6556个下调,GO富集于11个生物过程,17个细胞成分和2个分子功能,在氮代谢途径中共有15个DEGs,8个上调,7个下调；未施氮的叶和根中共有12178个DEGs,6268个上调,5910个下调,GO富集于7个生物过程,21个细胞成分和2个分子功能,在氮代谢途径中共有19个DEGs,8个上调,11个下调；氮代谢途径鉴定了编码9种基因的19个DEGs,7个DEGs在叶片中的表达量较高(PGSC0003DMG400016996,PGSC0003DMG400006913,PGSC0003DMG400030212,PGSC0003DMG400025823,PGSC0003DMG400016001,PGSC0003DMG400004355,PGSC0003DMG400009698),同时有7个DEGs在根中的表达量较高(PGSC0003DMG400015734,PGSC0003DMG400001145,PGSC0003DMG400008262,PGSC0003DMG400008356,PGSC0003DMG400014592,PGSC0003DMG400013235,Novel02273)。【结论】因此NRT2.4,NRT2.5,NRT2.7,NR,NiR基因主要参与马铃薯氮素吸收功能,GdH,GS,GOGAT基因主要参与马铃薯氮素利用功能。 |
| 英文摘要: |
| 【Objective】In order to study the physiological and biochemical responses of potato treated with different nitrogen concentrations and explore the genes related to nitrogen metabolism. 【Method】Taking Yanshu No. 4 potato as the test material, the nitrogen efficiency, physiological and biochemical differences as well as the key period of growth and development of potato were studied by pot planting under nitrogen and non-nitrogen treatments.In addition, transcriptome sequencing was performed on the leaves and roots of potato at the budding stage to obtain differentially expressed genes of nitrogen metabolism.【Result】The results showed:Nitrogen treatment significantly increased potato nitrogen efficiency, soluble sugar content, soluble protein content, root activity, nitrate reductase activity and glutamine synthase activity. Meanwhile, the budding stage was a key period of potato growth and development. A total of 12,996 DEGs were upregulated and 6,556 were upregulated in leaves and roots treated with nitrogen. GO was enriched in 11 biological processes, 17 cell components and 2 molecular functions, and 15 DEGs were upregulated and 7 DEGs were upregulated in nitrogen metabolism pathways; A total of 12,178 DEGs were upregulated in leaves and roots without nitrogen application, 6,268 were upregulated and 5,910 were upregulated. GO was enriched in 7 biological processes, 21 cell components and 2 molecular functions, and 19 DEGs were upregulated, 8 DEGs were upregulated and 11 DEGs were upregulated in nitrogen metabolism pathways;The nitrogen metabolism pathway identified 19 DEGs encoding 9 genes, and 7 DEGs were highly expressed in leaves,(PGSC0003DMG400016996,PGSC0003DMG400006913,PGSC0003DMG400030212,PGSC0003DMG400025823,PGSC0003DMG400016001,PGSC0003DMG400004355,PGSC0003DMG400009698), meanwhile, 7 DEGs had higher expression in roots,(PGSC0003DMG400015734,PGSC0003DMG400001145,PGSC0003DMG400008262,PGSC0003DMG400008356,PGSC0003DMG400014592,PGSC0003DMG400013235,Novel02273). 【Conclusion】Therefore, NRT2.4, NRT2.5, NRT2.7, NR and NiR genes are mainly involved in nitrogen absorption function of potato, while GdH, GS and GOGAT genes are mainly involved in nitrogen utilization function of potato. |
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