| p>王铃钰,邢梦玉,张旭阳,等.海南砧用南瓜种质资源对黄瓜枯萎病抗性鉴定及转录组分析[J].广东农业科学,2024,(4-6):- |
|
PDF
HTML
海南砧用南瓜种质资源对黄瓜枯萎病抗性鉴定及转录组分析 |
| Hainan Pumpkin Germplasm Resources for Resistance Identification and Transcriptome Analysis of Cucumber Fusarium Wilt |
| 投稿时间：2023-12-13 修订日期：2024-02-27 |
| DOI： |
| 中文关键词: 黄瓜枯萎病 抗性机理 抗感砧木 转录组测序 差异表达基因 |
| 英文关键词: Cucumber Fusarium Wilt Mechanism of Resistance Susceptible and Resistant Rootstock Transcriptome Sequencing Differentially Expressed Genes |
| 基金项目:海南省重大科技计划项目(ZDKJ2021005) |
|
| 摘要点击次数: 43 |
| 全文下载次数: 0 |
| 中文摘要: |
| 摘要：【目的】：筛选出海南黄瓜枯萎病抗感南瓜砧木，分析差异表达基因，探究抗性机理。【方法】：对收集的21种南瓜砧木种质资源进行黄瓜枯萎病抗性鉴定。人工接种尖孢镰刀菌黄瓜专化型（Fusarium oxysporum f. sp. Cucumerinum, FOC），通过测定发病率，病情指数（Disease Index, DI）、疾病严重程度曲线下面积（Area under the disease severity index curve, AUDRC）、根系活力（Root Activity）、叶绿素相对含量SPAD值、相对电导率(Relative conductivity, REC)、丙二醛（Malondialdehyde, MDA）等指标，综合评价不同砧木对黄瓜枯萎病的抗性，选取抗感砧木进行转录组测序分析，以接菌2d,4d和6d的抗感砧木根部组织为材料，未侵染0d为对照组，分析接种不同时段抗性和感病砧木中的差异表达基因。【结果】：DI指标表明砧木3、4、5号为抗性砧木，指标DI，结合AUDRC筛选出抗性最好为4号砧木；高感砧木为12、18、19号。生理指标中，根系活力表现较好为3、4、5号砧木；叶绿素含量较高的品种为4、5、10号砧木；相对电导率较低为1、5号砧木；丙二醛含量较低的砧木品种为3、4、15号。综合各项指标以及市场应用普及性，选取4号抗性砧木‘金甲砧’和18号感病砧木‘金刚钻1901’进行转录组测序，共同差异表达基因（Differentially Expressed Genes, DEGs）主要富集在丝裂原活化蛋白激酶(Mitogen-activated protein kinase, MAPK)信号通路、淀粉和蔗糖代谢、苯丙烷生物合成等途径。差异表达基因分析发现抗性砧木‘金甲砧’上调差异表达基因主要富集在苯丙烷类生物合成、植物-病原体互作、MAPK信号通路等抗性相关通路。【结论】：综合DI-AUDRC、根系活力、SPAD、REC、MDA生理指标，3、4、5号为抗性砧木，4号砧木‘金甲砧’为综合抗枯萎病最佳砧木，可作为海南黄瓜枯萎病抗性砧木育种资源，在响应黄瓜枯萎病应答防御中，抗性砧木‘金甲砧’DEGs参与苯丙烷类生物合成、植物-病原体互作等抗病相关代谢通路，涉及到PAL、4CL、CCR、HCT等可能与枯萎病抗性相关的基因，提高自身枯萎病抗性。 |
| 英文摘要: |
| Abstract: [Objective] This study aims to identify resistant and susceptible pumpkin rootstocks for Hainan cucumber fusarium wilt and to analyze differentially expressed genes (DEGs) to investigate resistance mechanisms. [Methods] Resistance to cucumber fusarium wilt was evaluated in 21 collected rootstocks. Following artificial inoculation with Fusarium oxysporum f sp. cucumerinum (FOC), disease incidence, disease index (DI), area under the disease severity index curve (AUDRC), root activity, soil and plant analysis development (SPAD), relative conductivity, and malondialdehyde (MDA) levels were measured to assess the resistance of various rootstocks to cucumber fusarium wilt. Resistant and susceptible rootstocks were selected for transcriptome sequencing. Root tissues of resistant and susceptible rootstocks at 2, 4, and 6 days post inoculation were used for analysis, with uninoculated 0 days as the control group, to identify DEGs at different inoculation intervals. [Results] The DI indicated that rootstocks 3, 4, and 5 were resistant, with rootstock 4 exhibiting the best-combined resistance based on the DI–AUDRC. Highly susceptible rootstocks were identified as rootstocks 12, 18, and 19. In terms of physiological indicators, rootstocks 3, 4, and 5 exhibited good root activity. Rootstocks 4, 5, and 10 had higher SPAD. Lower REC was observed in rootstocks 1 and 5. Lower MDA content was found in rootstocks 3, 4, and 15. Considering all indicators and market adoption, resistant rootstock 4 ‘JINJIAZHEN’ and susceptible rootstock 18 ‘JINGANGZUAN 1901’ were chosen for transcriptome sequencing. The shared DEGs were mainly enriched in pathways such as the mitogen-activated protein kinase signaling pathway, starch and sucrose metabolism, and phenylpropanoid biosynthesis. Analysis of DEGs revealed that upregulated DEGs in the resistant rootstock ‘JINJIAZHEN’ were mainly enriched in phenylpropanoid biosynthesis, plant–pathogen interaction, and mitogen-activated protein kinase signaling pathway, which are related to resistance. [Conclusion] Integrating DI–AUDRC, root activity, SPAD, REC, and MDA physiological indicators, rootstocks 3, 4, and 5 were identified as resistant. Rootstock 4 ‘JINJIAZHEN’ was found to be the best comprehensive rootstock for FOC and can serve as a breeding resource for rootstocks resistant to cucumber fusarium wilt in Hainan. In the defense response to cucumber fusarium wilt, DEGs in the resistant rootstock ‘JINJIAZHEN’ participate in metabolic pathways related to disease resistance, such as phenylpropanoid biosynthesis and plant–pathogen interaction, involving potential FOC resistance–related genes, such as PAL, 4CL, CCR, and HCT, and enhancing the fusarium wilt resistance of ‘JINJIAZHEN’. |
|
查看/发表评论 下载PDF阅读器 |
|
|
|
