| 张钰玉,陈智雄,陈方,等.不同土壤条件下辣椒根际土壤微生物群落分布特征研究[J].广东农业科学,2024,(4-6):- |
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不同土壤条件下辣椒根际土壤微生物群落分布特征研究 |
| Investigation of Rhizospheric Microbial Community in Pepper under Varied Soil ConditionsYuyu Zhang1*, Zhixiong Chen1, Fang Chen1, Jinqiang Yan2 *,Junyu Wu1,Qingyuan Xi3,Shumei Ge1 |
| 投稿时间：2023-12-29 修订日期：2024-03-21 |
| DOI： |
| 中文关键词: 辣椒 土传病害 根际土壤 微生物多样性 群落结构 |
| 英文关键词: Pepper Soil-Borne Diseases Rhizospheric Soil Microbial Diversity Community Structure |
| 基金项目:贵州省教育厅青年科技人才成长项目 (黔教合KY字(2019)098)；广东省农业科学院高水平农科院建设人才项目(R2021YJ-YB2004, R2023PY-JX007)；贵阳学院博士科研启动(0221001002010133)；大学生创新训练项目 (S202310976018)。 |
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| 摘要点击次数: 33 |
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| 中文摘要: |
| 【目的】研究不同抗性辣椒品种根际微生物群落分布特征，以期为辣椒土传病害的生物防治提供依据。【方法】以辣椒感病品种HL99和抗病品种SSQ为实验材料，利用高通量测序技术，分析比较感病土和非感病土条件对不同抗病性辣椒根际土壤细菌及真菌的群落多样性、物种组成、相关性网络和功能的影响。【结果】不同抗病性辣椒品种对土壤的驯化效果不同，该结果可直接影响辣椒根际土壤微生物群落分布，进而影响辣椒的生长。病害环境可导致土壤细菌丰富度降低，真菌丰富度增加。在病害条件下，细菌群落有更明显的响应，但不同抗病性品种间细菌群落结构无明显差异，不同抗病性品种间真菌群落结构有明显的差异。但不同品种不同处理下，单一细菌或真菌属的相对丰度与辣椒生长表现间未显示出显著的相关性。相较而言，抗病品种能在根际募集到更多有益菌，并进一步形成其各自组内及组间的相关性网络结构，这可能是形成差异性菌群结构，并在病害条件下增强辣椒抗病性、促进抗病辣椒品种生长的原因。【结论】抗病辣椒品种根际的有益真菌和有益细菌形成的相关性网络结构，可增强辣椒对病害环境的承受力，改善其生长表现。本研究鉴定出的镰刀菌属、轮枝菌属、枝顶孢属、枝孢属、葡萄孢属，木霉属、踝节菌属、节丛孢属等真菌，芽孢杆菌属、链霉菌属和假单胞菌属细菌，及其各自组内及组间的相关性网络结构，将为土壤驯化研究、生防资源的筛选与定殖提供重要信息，为土传病害的生物防治提供重要的理论参考。 |
| 英文摘要: |
| 【Objective】This study endeavors to examine the distribution patterns of rhizospheric microbial communities in diverse resistant pepper varieties, laying the groundwork for investigating the biological control mechanisms of soil-borne diseases in peppers. 【Methods】 Employing the susceptible variety HL99 and the resistant variety SSQ as experimental materials, we utilized high-throughput sequencing technology to scrutinize and compare the impacts of diseased and non-diseased soil conditions on the diversity, species composition, correlation networks, and functions of bacterial and fungal communities in distinct disease-resistant pepper rhizospheric soils. 【Results】Diverse disease-resistant pepper varieties exhibit distinct effects on soil domestication, directly influencing the distribution of rhizosphere soil microbial communities and subsequently impacting pepper growth. Disease environments can result in a decrease in bacterial richness and an increase in fungal richness in the soil. Under disease conditions, the bacterial community exhibits a more pronounced response, but there is no significant difference in bacterial community structure between different disease-resistant varieties. However, a noticeable difference exists in the fungal community structure among different disease-resistant varieties. Nevertheless, under different treatments and varieties, the relative abundance of individual bacterial or fungal genera does not exhibit a significant correlation with pepper growth performance. In comparison, disease-resistant varieties can recruit a greater number of beneficial fungi in the rhizosphere, forming a correlated network structure within and between groups. This phenomenon may contribute to the formation of distinct microbial community structures, enhancing pepper resistance to diseases and promoting the growth of disease-resistant pepper varieties under disease conditions.【Conclusion】The correlated network structure established by beneficial fungi and bacteria in the rhizosphere of disease-resistant pepper varieties enhances pepper"s resilience to disease environments and optimizes its growth performance. The identification of fungi such as Fusarium, Verticillium, Acremonium, Cladosporium, Botrytis, Trichoderma, Talaromyces and Arthrobotrys, alongside bacteria such as Bacillus, Streptomyces, and Pseudomonas, elucidating their respective intra- and inter-group correlation network structures, will supply crucial information for soil domestication research, the selection and colonization of biocontrol resources, and serve as a fundamental theoretical reference for the biological control of soil-borne diseases. |
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