| 郭 义,李鹏燕,李敦松.荔枝蒂蛀虫不同地理种群遗传多样性的初步研究[J].广东农业科学,2024,(4-6):- |
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荔枝蒂蛀虫不同地理种群遗传多样性的初步研究 |
| Preliminary study on genetic diversity of different geographical populations of the litchi fruit borer, Conopomorpha sinensis (Bradly) |
| 投稿时间：2024-01-13 修订日期：2024-02-28 |
| DOI： |
| 中文关键词: 荔枝蒂蛀虫 地理种群 遗传多样性 遗传分化 简化基因组测序 |
| 英文关键词: Conopomorpha sinensis geographical populations genetic diversity genetic differentiation simplified genome sequencing |
| 基金项目:国家荔枝龙眼产业技术体系(CARS-32)；广州市科技计划项目农村科技特派员专题（2024E04J1256） |
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| 中文摘要: |
| 【目的】明确荔枝蒂蛀虫Conopomorpha sinensis不同地理种群的遗传结构特征、遗传多样性水平及分子变异情况，为其起源和演化研究提供科学证据，为不同区域的该虫防治提供参考信息。【方法】选用我国南方5个产区的荔枝蒂蛀虫作为材料，利用GBS（Genotyping-by-sequencing）技术对样本进行基因分型，再利用SAMTOOLS软件鉴定SNP位点，根据SNP基因分型结果，采用邻接法构建系统进化树，并进行主成分分析。【结果】利用GBS简化基因组测序技术对5个地区荔枝蒂蛀虫进行测序，共获得高质量的有效数据27.39 Gb，平均每个样本5.48 Gb，共获得高质量的单核苷酸多态性（SNPs）位点11 767个。主成分分析结果显示5个地区荔枝蒂蛀虫划分为2大类，四川泸州种群明显区分于其它4个种群。系统进化树分析表明四川泸州种群离其它种群亲缘关系较远。主成分分析和系统进化树分析都揭示了不同地理来源的荔枝蒂蛀虫在遗传水平上存在明显的差异。种群遗传多样性分析结果表明5个种群观测杂合度为0.2099~0.2531，期望杂合度为0.1937~0.2203，π为0.2495~0.2748，显示荔枝蒂蛀虫种群的遗传多样性水平较低。AMOVA分析结果表明，荔枝蒂蛀虫种群间的遗传变异（0.87%）小于种群内（99.13%），说明变异主要来源于种群内，而不是种群间。【结论】不同地理种群的荔枝蒂蛀虫遗传多样性水平较低，四川泸州种群遗传分化程度较高，与其它种群亲缘关系较远。 |
| 英文摘要: |
| 【Objective】To clarify the genetic structure characteristics, genetic diversity level and molecular variation of different geographic populations of Conopomorpha sinensis, so as to provide scientific evidence for the study of its origin and evolution, and provide reference information for the control of this pest in different regions.【Method】Using the C. sinensis from 5 southern production areas of our country as materials, Genotyping the samples using GBS (Genotyping-by-sequencing) technology, then identifying SNP locus with SAMTOOLS software, according to SNP genotyping results, phylogenetic tree was constructed by the adjacency method. Principal component analysis was performed.【Result】Using GBS simplified genome sequencing technology, we sequenced C. sinensis in 5 regions. A total of 27.39 Gb of high quality effective data were obtained, with an average of 5.48 Gb per sample. A total of 11 767 high quality single nucleotide polymorphism (SNPs) locus were obtained. The results of principal component analysis showed that C. sinensis was divided into 2 groups in 5 regions, and the Luzhou population in Sichuan was significantly different from the other 4 populations. Phylogenetic tree analysis showed that Luzhou population was far from other populations. Both principal component analysis and phylogenetic tree analysis revealed that there were obvious differences in genetic level among C. sinensis of different geographical origin. The results of genetic diversity analysis showed that the observed heterozygosity was 0.2099~0.2531, the expected heterozygosity was 0.1937~0.2203, π was 0.2495~0.2748, indicating that the genetic diversity level of C. sinensis population was low. The results of AMOVA analysis showed that the genetic variation between populations (0.87%) was smaller than that within populations (99.13%), indicating that the variation mainly came from within populations rather than between populations.【Conclusion】 The genetic diversity level of C. sinensis in different geographic populations was low, the genetic differentiation degree of Luzhou population in Sichuan was high, and the genetic relationship with other populations was distant. |
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