| 邱道寿,罗永坚,李长发,等.骨碎补大棚栽培品与野生品的广靶代谢组学比较分析[J].广东农业科学,2024,(4-6):- |
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骨碎补大棚栽培品与野生品的广靶代谢组学比较分析 |
| Comparative analysis of metabolomics between greenhouse cultivated and wild collected Rhizoma Drynariae |
| 投稿时间：2024-02-19 修订日期：2024-03-09 |
| DOI： |
| 中文关键词: 骨碎补 槲蕨 大棚栽培 野生药材 代谢组学 黄酮 |
| 英文关键词: Rhizoma Drynariae Drynaria fortune Greenhouse cultivation Wild medicinal herbs Metabolomics Flavone |
| 基金项目:广东省省级科技计划项目“药用及药食两用作物种质资源收集、鉴评与利用(2019B030316008)”；“粤北山区道地南药生态种植技术研究与示范(200831086270718)”；广东省农业科研类及技术推广示范类项目“广东省南药产业运行情况监测(粤财农〔2022〕16号)”。 |
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| 中文摘要: |
| 【目的】骨碎补是一种多年生传统中药材,目前药材原料多来源于野生采集,鲜见有人工栽培的报道。因其野生资源采集、破坏严重,药材资源锐减,人工种植成为必然趋势。本研究在探索骨碎补设施大棚栽培技术的基础上,利用代谢组学技术开展了设施栽培药材与其野生品的靶向代谢组学比较研究,以期为骨碎补的人工栽培技术和栽培效果提供理论依据。【方法】本研究以骨碎补基原植物槲蕨为研究对象,采用UPLC-MS/MS分析技术对比分析了设施大棚栽培三年的槲蕨根茎与其野生根茎之间的差异代谢物,并进行了差异代谢物富集通路分析。【结果】从两种药材检测到的749种代谢物中筛选到100种差异代谢物。与野生品比较,在大棚栽培品中上调的58种,下调的42种。这些差异代谢物主要包括黄酮类化合物、有机酸、氨基酸及其衍生物、酚酸类、生物碱、游离脂肪酸等代谢物。其中,在大棚栽培品中,黄酮醇、黄酮碳糖苷、糖及糖类代谢物全部上调,而在野生品中,氨基酸及衍生物、酚酸类、溶血磷脂酰胆碱和花青素类代谢物上调较多。进一步的通路富集分析结果显示,100个差异代谢物共注释到47条代谢通路,其中,显著富集的有维生素B6代谢、芪类化合物生物合成、二芳基庚烷生物合成、姜酚的生物合成、黄酮和黄酮醇生物合成、半胱氨酸和蛋氨酸代谢、花青素生物合成、氨酰基tRNA生物合成等途径。【结论】大棚栽培的骨碎补,其黄酮醇、黄酮碳糖苷、糖及糖类代谢物化合物显著多于野生品,鉴于骨碎补总黄酮被公认为主要药效成分,因此,大棚栽培的骨碎补比野生品具有主效成分优势,可为骨碎补规模化设施栽培或人工栽培提供科学依据。 |
| 英文摘要: |
| 【 Objective 】 Rhizoma Drynariae is a perennial traditional Chinese medicinal herb. Currently, most of the medicinal materials come from wild collection, and there are few reports of artificial cultivation. Due to the serious collection and destruction of its wild resources, medicinal resources have sharply decreased, and artificial cultivation has become an inevitable trend. On the basis of attempting greenhouse cultivation techniques for Rhizoma Drynariae, this study utilized metabolomics techniques to conduct targeted metabolomics comparative studies between greenhouse cultivated materials and wild collected materials, in order to provide theoretical basis for the artificial cultivation techniques and cultivation effects of Rhizoma Drynariae.【 Method 】This study focuses on the original plant of Rhizoma Drynariae, Drynaria fortune, and uses UPLC-MS/MS analysis technology to compare and analyze the differential metabolites between greenhouse cultivated three-year rhizomes and its wild three-year rhizomes. Subsequently, enrichment pathway analysis of differential metabolites was conducted using the KEGG pathway database. 【 Result 】 Out of 749 metabolites detected from two habitat samples, 100 differential metabolites were screened. Compared with wild samples, there were 58 upregulated metabolites and 42 downregulated metabolites in greenhouse cultivation samples. These differential metabolites mainly include flavonoids, organic acids, amino acids and their derivatives, phenolic acids, alkaloids, free fatty acids, and other metabolites. Among them, in greenhouse cultivation samples, flavonols, flavonoid glycosides, sugars, and carbohydrate metabolites were all upregulated, while in wild samples, amino acids and derivatives, phenolic acids, lysophosphatidylcholine, and anthocyanin metabolites were more upregulated. Further pathway enrichment analysis showed that 100 differential metabolites were annotated into 47 metabolic pathways, among which significantly enriched pathways included vitamin B6 metabolism, astragalus compound biosynthesis, diarylheptane biosynthesis, gingerol biosynthesis, flavonoid and flavonol biosynthesis, cysteine and methionine metabolism, anthocyanin biosynthesis, and aminoacyl tRNA biosynthesis.【Conclusion】 The content of flavonols, flavonoid glycosides, sugars, and carbohydrate metabolites in greenhouse cultivated Rhizoma Drynariae is significantly higher than that in wild cultivated Rhizoma Drynariae. Considering that the total flavonoids of Rhizoma Drynariae are recognized as the main active ingredients, greenhouse cultivated Rhizoma Drynariae has the advantage of main active ingredients compared to wild cultivated Rhizoma Drynariae, which can provide scientific basis for large-scale facility cultivation or artificial cultivation of Rhizoma Drynariae. |
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