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<title cf:type="text"><![CDATA[Editorial Department of Guangdong Agricultural Sciences -->Plant Protection]]></title>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Research Progress on Mechanisms of Pineapple Blackheart]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201909013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[Pineapple blackheart is one of the major diseases after harvesting. The damaged fruits usually have no 
obvious external characteristics and are hard to be distinguished from the normal ones. The initial symptoms of pineapple 
blackheart are translucent spots appearing near the fruit core. With the development of the internal browning, dark-brown 
pigmentation begins to appear in the pulp and gradually spreads to the whole fruit. In recent years, due to the abuse of 
gibberellins or other reasons, blackheart has been reported to appear in summer fruits of pineapples, causing huge economic 
losses to the whole pineapple industry. Scholars at home and abroad have studied the underlying mechanisms of pineapple 
and proposed various solutions. Therefore, summary of the current research progresses have positive influence on studying the mechanisms of pineapple blackheart, breeding blackheart resistant cultivars and improving the production efficiency of 
the pineapple industry. This paper summarized the pre-harvest and post-harvest factors affecting the occurrence of pineapple 
blackheart and the corresponding countermeasures, and proposed a possible model of pineapple blackheart based on the recent 
progresses of related hormone signaling pathways. The model can be divided into three stages, namely, initial stage, apoptosis 
execution stage and browning stage. Chilling stress is a major factor of internal browning, which induces ROS and causes 
membrane damage in the initial stage of pineapple blackheart. Gibberellins act in the downstream of chilling stress, which play 
an important role in the apoptosis execution stage. The browning reaction catalyzed by PPO in the browning stage might be only 
a secondary response. There is no direct correlation between the level of PPO and the incidence of pineapple blackheart.]]></description>
<pubDate>2019/11/15 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[贺 涵，刘传和，匡石滋，肖维强，赖 多，邵雪花]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>贺 涵，刘传和，匡石滋，肖维强，赖 多，邵雪花</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201909013&flag=1]]></guid><cfi:id>119</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effects of Adding Chemical Fertilizer and Plastic Films to Raise Soil Temperature on the Elimination of Radopholus similis in Soil]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201909014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The eliminating effect of chemical fertilizer and sunlight on Radopholus similis in soil 
were studied.【Method】The experiment was conducted by adding chemical fertilizer to the soil infected with R. similis
and covering it with plastic films.【Result】The results showed that the soil temperature was raised by adding calcium 
cyanamide or carbamide and covering the soil with white or black films. In the case that the highest average temperature in 
the local area was above 38.8 ℃ , and the number of sunny days was above 31 days, the R. similis in soil could be eliminated 
effectively by the method(adding calcium cyanamide or carbamide and covering soil with films) treating soil for no less than 
5 weeks; in the case that the highest average temperature in the local area was above 37.6 ℃ , and the number of sunny days 
was above 38 days, the R. similis in soil could also be eliminated by the method(adding calcium cyanamide or carbamide and 
covering soil with films) treating soil for no less than 6 weeks. Among all the treatments, 1 L soil treated with 10 g calcium cyanamide and covered with white films had the best effect on increasing soil temperature.【Conclusion】In high temperature 
seasons, the method of adding calcium cyanamide or carbamide and covering white or black films can eliminate the R. similis in 
infected soil.]]></description>
<pubDate>2019/11/15 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[吕 梅，杨 欣，李俊毅，丁善文，徐春玲，谢 辉]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>吕 梅，杨 欣，李俊毅，丁善文，徐春玲，谢 辉</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201909014&flag=1]]></guid><cfi:id>118</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Insecticidal Activity and Behavior Inhibition of the RedImported Fire Ant by Strophanthus divaricatus]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201909015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was conducted to explore the effects of Strophanthus divaricatus on the toxic 
activity, climbing ability and walking ability of Solenopsis invicta Buren.【Method】The toxic activity of S. divaricatus 
against the red imported fire ant (RIFA)was assayed with water immersion soil treatment method.【Result】S. divaricatus
had good toxic activity and behavioral inhibition on small and medium RIFA workers. After treated with fresh smashed 
leaves of S. divaricatus (0.5 g), the mortality rates of small RIFA workers with soil depths of 0-1.2 cm, 1.2-2.4 cm and 2.4-3.6 
cm were 80.00%, 60.00% and 85.33%, and those of medium RIFA workers were 37.33%, 34.67% and 40%. After treated 
with fresh smashed leaves of S. divaricatus (1 g), the mortality rates of small RIFA workers were 50.67%, 62.67% and 88% 
respectively, and those of medium RIFA workers were 33.33%, 33.33% and 40%. After treated with fresh smashed leaves of 
S. divaricatus (2 g), the mortality rates of small RIFA workers were 90.67%, 88%, 73.33%, and those of major RIFA workers 
were 18.67%, 28%, 12%, respectively.【Conclusion】S. divaricatus has a good lethal effect on small and medium RIFA 
workers and it can significantly reduce their walking ability and climbing ability. It has stronger insecticidal activity and 
behavioral inhibition on small RIFA workers than that on medium RIFA workers. S.divaricatus is expected to become a new 
material for the control of RIFA.]]></description>
<pubDate>2019/11/15 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[黄玲俊，肖春霞，张志祥]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>黄玲俊，肖春霞，张志祥</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201909015&flag=1]]></guid><cfi:id>117</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effect of 30% Xiange Oceanic Bioactive Peptides PesticideFertilizer on Sugarcane Pest Prevention, Yield and Sugar]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201909016&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The objective was to study the effect of 30% Xiange oceanic bioactive peptide pesticidefertilizer on sugarcane pests prevention, yield and sugar.【Method】With conventional compound fertilizer and 3% 
carbofuran granulated insecticide as control, a comparative test of the field effect of 30% Xiange pesticide fertilizer on 
sugarcane was carried out.【Result】The results showed that after the application of 30% Xiange pesticide-fertilizer for 90 
d, the control rates on sugarcane borer in the two test points were 93.66% and 93.09%, respectively, which were significantly 
higher than the rates of 21.86% and 24.19% of the control. Before harvest, the reduction rates of sugarcane borer in the two 
test points under pesticide-fertilizer treatment were 6.21% and 5.10%, respectively, which were significantly lower than the rates of 36.25% and 34.55% of the control. The control rates of Xiange pesticide-fertilizer on sugarcane chafer in two test 
points were 91.34% and 100.00% respectively, which were significantly higher than the rates of 68.00% and 64.29% of the 
control. The yields of sugarcane treated with Xiange pesticide-fertilizer at two test points were 107 700 kg/hm2 and 108 000 kg/
hm2, respectively, with the sugar contents of 15.62% and 15.55%, respectively. The yield and sugar content of sugarcane treated 
with Xiange pesticide-fertilizer increased by 28.63% and 1.33% compared with those of the conventional control treatment.
【Conclusion】30% Xiange oceanic bioactive peptide pesticide-fetilizer has good effect on sugarcane pests control, which 
would obviously increase yield and sugar. It is worth being promoted and applied in sugarcane planting area.]]></description>
<pubDate>2019/11/15 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[谢江江 1
，罗青文 1
，吴　冠 2，杨春强 1
，陈　聪 2，杨马生 2，陈　顺 1
，杨俊贤 1，2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>谢江江 1
，罗青文 1
，吴　冠 2，杨春强 1
，陈　聪 2，杨马生 2，陈　顺 1
，杨俊贤 1，2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201909016&flag=1]]></guid><cfi:id>116</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effects of Rice Dwarf Virus on the Development andReproduction of Nephotettix cincticeps (Uhler)]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201908013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Rice dwarf disease is a kind of common viral disease, which causes serious threats to rice
production. Rice dwarf virus (RDV) is the pathogeny and the spread of the disease is mainly rely on Nephotettix cincticeps
(Uhler) in a persistent-propagative manner. The exploration on the effects of RDV on the vector insect green rice leafhopper
can provide basis for the population control of N. cincticeps and the sustainable control of rice dwarf disease.【Method】Two
treatments(non-infected and RDV-infected) were set at 27℃ in laboratory, the growth and population increase of N.cincticeps
were observed and the life tables of experimental populations were constructed.【Result】The results showed that the nymph
survival, fresh body weight per male/female adult of N.cincticeps fed on RDV-infected rice plants did not significantly differ
from those on healthy plants, longevity and fecundity of female adult were slightly longer/higher, and the population number
of adults was significantly increased, whereas the growth period of male/female nymph and longevity of male adult was
decreased obviously. Also, the net reproductive rate (R0) significantly increased among the five life table parameters.
【Conclusion】RDV-infected rice plants has positive indirect effects on N.cincticeps and RDV is beneficial to the population increase of N.cincticeps]]></description>
<pubDate>2019/9/24 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[王前进 1，2，方　琦 2，叶恭银 2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>王前进 1，2，方　琦 2，叶恭银 2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201908013&flag=1]]></guid><cfi:id>115</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[A SYBR Green Ⅰ Real-time Fluorescence Quantitative PCR Detection Method for the Mulberry MosaicDwarf associated Virus]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201908015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The purpose of this study was to establish a real-time fluorescence quantitative PCR
detection method for the Mulberry Mosaic Dwarf associated Virus (MMDaV).【Method】As target gene，the conservative
coat protein gene of MMDaV was applied to design the specific primers，construct the positive plasmids of MMDaV and
establish the standard curve of the positive plasmids.The sensitivity and specificity of the particular primer of MMDaV
was detected.The real-time fluorescence quantitative PCR detection method of MMDaV was applied to detect MMDaV of
diseased mulberry leaf from six provinces of Guangdong，Guangxi，Hainan，Chongqing，Shaanxi，and Jiangxi.【Results】
The standard curve constructed has excellent amplification efficiency (97.88%).The specific primer can accurately detect
the MMDaV.The detection method has the lowest plasmid detection concentration of 8 copies/μL，the sensitivity of which
was 24 times higher than that of conventional PCR.The method has good detection results for diseased mulberry leaf samples
from six provinces，and the Cq value of detection is between 9.69 and 26.17.【Conclusion】The established real-time
fluorescence quantitative PCR detection method for MMDaV，with high efficiency，specificity and sensitivity，can be
applied to detect MMDaV in host plants quantitatively.]]></description>
<pubDate>2019/9/24 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[孙勋勋，杨宏宇，周轶楠，刘吉平]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>孙勋勋，杨宏宇，周轶楠，刘吉平</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201908015&flag=1]]></guid><cfi:id>114</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Identification of Pathogens and Their Virulence of Crown Gall Disease of Peach from Different Peach Production Regions]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201907013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】 In this study, the pathogenicity of Agrobacterium tumefaciens, a pathogen isolated from
tumor tissues collected from several peach production regions, was identified. 【Method】Molecular detection of virD2 and
ipt genes associated with A. tumefaciens and T-DNA metastasis and gall tumor formation was carried out. The pathogenicity
of A. tumefaciens isolated from tumor tissues collected from different peach production regions was identified by means of
the 3-ketoglycosides test and other biological methods. Indicator plants were used to detect the pathogenicity of strains of
different biological types. Finally, peach plants of different varieties was used to verify the pathogenicity of crown gall strains
by artificial inoculation. 【Result】 The PCR results showed that there are both 203 bp and 427 bp target fragments of the
specific primers virD2 and ipt genes in 21 strains of pathogenic bacteria, Which indicate that the isolated pathogens belong
to A. tumefaciens. The results of 3-ketoglycosides test show that 3 strains changed from blue to yellow belong to Biotype Ⅰ ,
and the remaining 18 strains with unchanged color belong to Biotype Ⅱ . Indicator plants show that pathogens could form
tumors on plants, and the pathogenicity of Biotype Ⅱ strains is significantly stronger than that of Biotype Ⅰ . The inoculation
of Biotype Ⅱ pathogens in different varieties of peach trees can form tumors obviously, and it also verify that Biotype Ⅱ
pathogens have stronger pathogenicity. 【Conclusion】 The pathogens of crown gall disease isolated from different peach
production areas are A. tumefaciens, most of which belong to Biological biotype Ⅱ , which has strong pathogenicity and can infect almost all peach varieties.]]></description>
<pubDate>2019/8/22 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[郝峰鸽，李桂荣，王保全，蔡祖国，尤　杨，牛生洋]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>郝峰鸽，李桂荣，王保全，蔡祖国，尤　杨，牛生洋</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201907013&flag=1]]></guid><cfi:id>113</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effect of Polysiloxane-polyacrylate QuaternaryAmmonium Salt Block Copolymer onCell Membrane of Rhizoctonia solani]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201907014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was to investigate the effect of polysiloxane-polyacrylate quaternary ammonium
salt block copolymer (Six-Q5) on cell membrane of Rhizoctonia solani, and to provide a new idea for the control of rice sheath
blight.【Method】The effects of Six-Q5 on cell membrane integrity of R. solani were observed under differential interference
microscopy after PI staining. The effects of Six-Q5 on cell membrane permeability of R. solani were characterized by
Coomassie brilliant blue method, anthrone-sulfuric acid method and conductivity method, respectively. Malondialdehyde
(MDA) kit was used to determine the content of MDA to characterize the effect of Six-Q5 on lipid peroxidation of cell
membrane of R. solani.【Result】Under the fluorescence microscope, the mycelia treated with Six-Q5 are observed with
fluorescent bright spots. It is found that the protein, carbohydrate and electrolyte contents in the mycelium culture medium of the treatment are significantly different from those of the control. The MDA content of mycelia and culture medium in the
treatment increased with the increas of drug concentration. With the difference of PDMS block length, its influence on the R.
solani mycelia membrane is also different.【Conclusion】The results show that Six-Q5 destroy the integrity of cell membrane,
chang the permeability of cell membrane and the structural and functional integrity of cell membrane. Moreover, with the
increase of the block length of Six-Q5, the effect is more pronounced. Cell membrane is a target of Six-Q5 for inhibiting rice
sheath blight.]]></description>
<pubDate>2019/8/22 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[古广武 1
，董辰韵 1
，钟伟强 2，张安强 2，林雅铃 1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>古广武 1
，董辰韵 1
，钟伟强 2，张安强 2，林雅铃 1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201907014&flag=1]]></guid><cfi:id>112</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Screening of Formula for Seed Pelleting Agentof Bacillus subtilis Yz strain]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201907015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】This study aims to clarify the bacteriostatic activity of the fermentation powder of Bacillus
subtilis Yz strain on cotton seedling diseases, and screens the formula for seed pelleting agent with the decline rate of live
spores and the germination rate of cotton seeds as indicators.【Method】The antibacterial activity of Yz strain fermentation
powder against cotton damping-off, cotton anthracnose, and cotton red rot was determined by the confrontation culture
method. In addition, by orthogonal design L9(33), the effects of different additives on the decline rate of live spores of
fermentation powder of B. subtilis Yz strain and the germination rate of cotton seeds were determined to screen the best
formula for seed pelleting agent.【Result】The corrected inhibitory zone diameter of fermentation powder of B. subtilis
Yz strain with 50 times dilution for cotton damping-off, cotton anthracnose, and cotton red rot were 10.07mm, 15.54mm
and 8.96mm, respectively. And the diameter of inhibitory zone decreased gradually with the decrease of the concentration
of fermentation powder. The best additives were diatomite, sodium carboxymethyl cellulose and sodium lignosulfonate.
【Conclusion】The fermentation powder of B. subtilis Yz strain show good antibacterial activity against cotton dampingoff,
cotton anthracnose, and cotton red rot. The optimum proportion is 3(diatomite) ∶ 0.8(sodium carboxymethyl
cellulose) ∶ 0.1(sodium lignosulfonate) ∶ 1(Yz fermentation powder).]]></description>
<pubDate>2019/8/22 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[罗振亚 1
，徐　淑 1
，林开春２]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>罗振亚 1
，徐　淑 1
，林开春２</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201907015&flag=1]]></guid><cfi:id>111</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Preliminary Study on the Behavior Rhythmof Bandicota indica in Summer]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201907016&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】In order to elucidate the seasonal variation trend of the activity rhythm of Bandicota
indica, the behavior rhythm of B. indica in rat pond under high temperature in summer was studied.【Method】One rat
was cultured in every pond and infrared camera monitoring system was used to automatically record all kinds of behavior
of B. indica in the pond.【Result】In hot summer, B. indica began to leave the nest and forage at 18：00 p m and such
activities lasted until 4：00 a m the next day. The peak period of the activity out of the nest appeared between 19：00
and 3：00 a m. In the nest the B. indica had behaviors such as resting, sleeping, self-modification, looking around and
burrowing. 82.82% of the time was used for resting and sleeping, accounting for 54.24% of the total time of activities the
whole day. There were many activities out of nest, such as looking around, running, feeding, drinking, self-modification
and burrowing. The main activities were looking around and running, accounting for 38.58% and 34.75% of the total time
of out-of-nest activities, respectively. The behavior rhythm of feeding and drinking was basically the same, and the total
time of feeding was significantly higher than that of drinking. Self-modification and burrowing were evenly distributed
throughout the day, and 53.54% of the modification activities took place out of the nest.【Conclusion】B. indica spent most of time resting in the nest during the day, and activities were carried out at night, the activity rhythm of which belonged
to the type of hiding in the daytime and coming out at night. There were some differences in time distribution between the
activities inside and outside the nest.]]></description>
<pubDate>2019/8/22 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[姚丹丹，隋晶晶，姜洪雪，冯志勇]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>姚丹丹，隋晶晶，姜洪雪，冯志勇</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201907016&flag=1]]></guid><cfi:id>110</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Characterization of Antibacterial Activity of Amphipathic Macromolecular Quaternary Ammonium Salts Against Rhizoctonia solani]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201906012&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】This study provided a reference for the selection of characterization methods of amphiphilic 
macromolecule quaternary ammonium salts (QAS) on plant pathogenic fungi.【Method】The antifungal activity of 
new amphipathic macromolecular QAS copolymers 〔PDMS-b-(PDMAEMA-BC), labeled as Six-Q5〕, water soluble 
macromolecule QAS (PQD-BC) and small molecular QAS (benzalkonium chloride, BC) against Rhizoctonia solani were 
characterized by growth rate method and mycelium weighing method respectively.【Result】Growth rate method can be 
used to characterize the antimicrobial activity of hydrophilic macromolecular QAS (PQD-BC) and small molecular QAS 
(BC), but not used to characterize amphiphilic macromolecular QAS. Mycelial weighing method can be used to characterize 
the antimicrobial activity of three kinds of QAS. The IC50 and IC90 of Si5-Q5 with moderate length of PDMS block for R. 
solani were 12 μg/mL and 59 μg/mL respectively, and its antifungal activity was similar to that of small molecular QAS.
【Conclusion】 For amphipathic macromolecule QAS, mycelial weighing method was more suitable than growth rate method for the characterization of antifungal activity.]]></description>
<pubDate>2019/8/8 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[董辰韵 1
，常瑶瑶 1
，钟伟强 2，张安强 2，林雅铃 1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>董辰韵 1
，常瑶瑶 1
，钟伟强 2，张安强 2，林雅铃 1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201906012&flag=1]]></guid><cfi:id>109</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Study on Life Table of Laboratory Population of Amblyseiuscucumeris Oudemans Preying on Scirtothrips dorsalis Hood]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201906013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was to clarify the growth, development and propagation of Amblyseius cucumeris 
Oudemans with Scirtothrips dorsalis Hood as prey, and the population growth parameters were obtained, which provided 
references for the control of S. dorsalis by using A. cucumeris. 【Method】The development duration, survival rate, adult 
longevity and fecundity of A. cucumeris with S. dorsalis as prey were observed indoors by using the traditional biological 
observation method, the life table of the laboratory population was established, and the life table parameters were analyzed. 
【Result】The results showed that the feeding amount of female adult A. cucumeris was the largest, up to 122 individuals, 
follow by that of nymph and non-feeding of larvae. The development period of A. cucumeris with S. dorsalis as prey was 9.98 
d, and the survival rate was 94%. The average longevity of adult female was 24 d, and the average per female oviposition was 28, and a large number of adult deaths occurred after the peak of oviposition. The net reproductive rate (Ro), intrisic rate of 
natural increase (rm), mean generation time (T), finite rate of increase (λ) and double population time (t) of A. cucumeris were 
26.95, 0.15, 22.32 d, 1.16 and 4.70 d, respectively. 【Conclusion】With S. dorsalis as prey, A. cucumeris can complete its 
generation growth, development and reproduction, and can be used as a predatory enemy of S. dorsalis for its biological control.]]></description>
<pubDate>2019/8/8 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[郑丽旧1
，陈俊谕 2,3，王康权 1
，符悦冠 2，章程辉 3]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>郑丽旧1
，陈俊谕 2,3，王康权 1
，符悦冠 2，章程辉 3</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201906013&flag=1]]></guid><cfi:id>108</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Screening of Detoxication for Tobacco Phytotoxicity Caused by Insecticides and Herbicides]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201906014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】 The study was to screen detoxications to phytotoxicity caused by insecticides and herbicides 
on tobacco.【Method】The pot experiment was conducted to study the detoxication effects to tobacco photytoxicity. 【Result】 
Microelement fertilizer had good repair effect to the phytotoxicity of methomyl and phoxim. The repair rates were 47.28% 
and 54.43%, respectively after 9 days treatment. Brassinolide and amino acid fertilizer could relieve the phototoxicity of 
imidacloprid . After 9 days treatment, the repair rates were 60.32% and 56.77%, respectively. Active carbon and quick lime 
could repair deformity of tobacco by quinclorac. When the concentration of active carbon in soil was 80mg/kg, the repair 
rate of the plant height, leaf length and leaf width were 81.44%, 76.81% and 89.59% after 30 days treatment, and the repair 
rate increased with the increase of the concentration of active carbon in soil. Quick lime had the best detoxication effect on quinclorac when the concentration was 150 mg/kg in soil, but the repair rate decreased with the increase of the concentration of 
quick lime . Mefenpyr-diethyl and active carbon could relieve bensulfuron-methyl’s inhibition on growth of tobacco. Active 
carbon and mefenpyr-diethyl had good repairing effects on bensulfuron-methyl. Mefenpyr-diethyl had the best detoxication 
effect on bensulfuron-methyl when its concentration was 4 mg/kg in soil. The repair effect decreased with the increase of the 
concentration of mefenpyr-diethyl. The detoxication effect of active carbon on bensulfuron-methyl phototoxicity increased with 
the increase of its concentration in soil. 【Conclusion】 Microelement fertilizer could be use to alleviate the phototoxicity of 
methomyl and phoxim. Brassinolide and amino acid fertilizer could relieve the imidacloprid phototoxicity on tobacco. Active 
carbon and quick lime could repair the tobacco phytotoxicity by quinclorac. Bensulfuron-methyl phototoxicity could be 
alleviated by active carbon and mefenpyr-diethyl.]]></description>
<pubDate>2019/8/8 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[沈会芳，蒲小明，张景欣，孙大元，林壁润]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>沈会芳，蒲小明，张景欣，孙大元，林壁润</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201906014&flag=1]]></guid><cfi:id>107</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Investigation on Species of Hemiptera Pests Attacking Mulberry in Guangdong and Their Damage]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201905012&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】In recent years, Hemiptera pests are the main pests in mulberry planting area in Guangdong, 
which cause great economic losses to sericulture production every year. However, there is no relevant research on the 
systematic identification of Hemiptera mulberry pests and damage investigation of such pests in Guangdong. Therefore, it is 
of great significance to systematically investigate and study the Hemiptera mulberry pests in Guangdong Province, so as to 
provide theoretical basis for further formulating effective control measures and carrying out relevant research. 【Method】
The investigation and identification of seven Lepidoptera pests in mulberry orchard was systematically conducted by 
morphology and molecular characteristics (cytochrome oxidase subunit Ⅰ gene, mtCO Ⅰ ).【Result】Seven species of Hemiptera 
pests were identified, i.e., Ferrisia virgata Cockerell, Anomoneura mori Schwarz, Erythroneura mori Matsumura, Aphrophora 
sp., Nezara viridula Linnaeus, Acanthocoris scaber Linnaeus, Dysdercus cingulatus Fabricius. Ferrisia virgata Cockerel. 
【Conclusion】Among the seven identified Hemiptera pests, Aphrophora sp. and Dysdercus cingulatus Fabricius were first 
reported to attack mulberry in Guangdong.]]></description>
<pubDate>2019/7/1 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[章 攀 1，2，游春平 2，邵蝴蝶 2，韩群鑫 2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>章 攀 1，2，游春平 2，邵蝴蝶 2，韩群鑫 2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201905012&flag=1]]></guid><cfi:id>106</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Comparative Test on Trapping Effects of Different Sex Pheromone Luresto Spodoptera litura in Guangzhou]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201905013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】A comparison of the attractive effects of different sex attractants on Spodoptera litura in 
Guangzhou was conducted. 【Method】 The trapping effects of four kinds of S. litura lures (NB, QZ, BJ, ZZ) from different 
manufacturers was measured in fields of Guangzhou. When other external factors, such as crops and field management 
measures, were basically the same, the number of S. litura trapped was collected every other week, and the lures were 
replaced every four weeks. The significant difference analysis was conducted on the number of S. litura trapped by the four 
lures for S. litura. 【Result】The total number of S. litura attracted by NB, QZ, BJ and ZZ were 2402, 786, 394 and 123 
respectively. The number of S. litura trapped by lure NB was 3.05 times of that by lure QZ, 6.09 times of that by lure BJ 
and 19.53 times of that by lure ZZ. The average number of S. litura attracted per bottle per day was 26.1, 8.5, 4.2 and 1.3 
respectively. The number of S. litura trapped by lure NB was 3.07 times of that by lure QZ, 6.21 times of that by lure BJ, and 
20.08 times that by lure ZZ.【Conclusion】 The lure activity: NB > QZ > BJ > ZZ; and the half-life time: NB(32.25d) ＞
QZ(25.1d) ＞ BJ(16.04d) ＞ ZZ(12.1765d).]]></description>
<pubDate>2019/7/1 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[陈炳翰，温焰辉，程传宝，杨云惠，申建梅]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>陈炳翰，温焰辉，程传宝，杨云惠，申建梅</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201905013&flag=1]]></guid><cfi:id>105</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effect of Ultrasonic Wave on Microorganisms on the Surfaces of Rice Seeds]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201905014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Ultrasound wave (USW) treatment of rice seeds can promote seed germination and seedling 
growth, but its bactericidal effect is not clear. In this study, we intend to investigate the effects of USW on fungi and bacteria 
on the surface of rice seeds, and to evaluate the antimicrobial efficacy of different USW treatments and the influencing 
factors. We also try to analyze the reasons of the promotion of rice germination and seedling growth by USW. 【Methods】 Using the rice cultivar, Xiangyaxiangzhan, as material, three treatments, 25-50 kHz USW wet method (Twet), 50 kHz USW drying 
method (Tdry1) and 25 kHz USW drying method (Tdry2), were set up. The conventional wet method (CK1) and dry method (CK2) 
were taken as controls. The experiments were repeated three times. Microorganisms on the seed surfaces were separated by 
PDA plates. The fungi and bacteria were identified by combining colony features, microscopic structures and ITS sequences 
analysis. 【Results】USW had substantial bactericidal and fungicidal effects on seed surfaces. Wet treatment of USW (Twet) 
was better than dry treatment (Tdry1, Tdry2), and the 50 kHz USW (Tdry1) was better than 25-kHz USW (Tdry2). Among the 15 
species of bacteria detected on seed surfaces, USW had the significant bactericidal effects on low abundance bacterial species 
such as Xanthomonas sacchari, Pseudomonas aeruginosa, Chryseobacterium gleum, Pantoea agglomerans and Stenotrophomo 
maltophilia, but USW had not effects on the higher abundance bacteria such as Enterobacter cloacae and Klebsiella pneumoniae. 
Eleven fungi were detected on the seed surfaces, USW had better fungicidal effects on Fusarium proliferatum, F. oxysporum, 
F. moniliforme, F. equiseti and Phoma sp. But USW had not apparent effects on Sarocladium oryzae and Penicillium 
oxalicum with the relatively higher richness. 【Conclusion】USW has antimicrobial effects on rice seed surfaces, but the 
effects are related to the treatment ways of USW, microorganism species and their abundance. USW treatment on wet seeds has 
better bactericidal and fungicidal effects than those of USW treatment on dry seeds. USW has significant fungicidal effects on 
Fusarium spp with low abundance, which may be one of the reasons for promoting rice germination and seedling growth.]]></description>
<pubDate>2019/7/1 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[蔡　伟 1
，严卓晟 2，William Jia Lan3，彭　彬 4，钟坚文 2，张　杰 2，胡琼波 1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>蔡　伟 1
，严卓晟 2，William Jia Lan3，彭　彬 4，钟坚文 2，张　杰 2，胡琼波 1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201905014&flag=1]]></guid><cfi:id>104</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Research Progress on Sugarcane Smut and Its Control]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201904012&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[Sugarcane is one of the important sugar and biomass energy crops in the world. Sugarcane smut caused
by Sporisorium scitamineum is the main disease of sugarcane, which results in enormous yield losses and decline in cane
quality, and poses a serious threat to the sustainable development of sugarcane production and sugar industry in China and
even in the world. Currently, study on pathogenic mechanism of S. scitamineum and resistance mechanism of sugarcane
at molecular level has become a research hotspot, which provides scientific basis for smut control and disease resistance
breeding of sugarcane. At present, breeding and promotion of new resistant varieties is the most economical and effective
measure to control sugarcane smut. However, research of breeding resistance cultivars is relatively weak in China. This paper
reviewed disease occurrence, pathogenic feature, genetic diversity, pathogenic mechanism, disease resistance breeding and
control measures. The further research ideas of sugarcane smut were also discussed according to current research.]]></description>
<pubDate>2019/5/23 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[韦金菊 1, 宋修鹏 1, 魏春燕 1, 张小秋 1, 黄伟华 2, 颜梅新 1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>韦金菊 1, 宋修鹏 1, 魏春燕 1, 张小秋 1, 黄伟华 2, 颜梅新 1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201904012&flag=1]]></guid><cfi:id>103</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effects of Chlorantraniliprole on Toxic Activity and Behavior of Solenopsis invicta Buren]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201904013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was conduct to explore the effects of chlorantraniliprole on the toxic activity,
walking ability, climbing ability and adhesion ability of small and medium-sized Solenopsis invicta Buren. 【Method】 The
toxic activity of chlorantraniliprole against S. invicta Buren was assayed with aqueous solution tubes method. 【Result】The
results showed that the corrected mortality of the small-sized S. invicta Buren was 96.55% after treatment with 8 mg/L of
chlorantraniliprole for 60 h. The corrected mortality of the medium-sized S. invicta Buren was 100.00% after treatment with
16 mg/L of chlorantraniliprole for 60 h. After treatment with 8 mg/L chlorantraniliprole for 36 h, the walking rate, climbing
rate and adhesion rate of the small-sized Solenopsis invicta Buren were 15.00%, 15.00% and 11.67%, respectively. After
treatment with 16 mg/L chlorantraniliprole for 36 h, the walking rate, climbing rate and adhesion rate of the medium-sized
Solenopsis invicta Buren were 15.00%, 13.33% and 11.67%, respectively. 【Conclusion】 Chlorantraniliprole shows good
toxic activity and behavior effects on small and medium-sized S. invicta Buren, and has a good application prospect in the
prevention and control of S. invicta Buren.]]></description>
<pubDate>2019/5/23 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[陈慧雅，黄诗悠，马千里，张志祥]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>陈慧雅，黄诗悠，马千里，张志祥</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201904013&flag=1]]></guid><cfi:id>102</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Application of Agrobacterium tumefaciens-mediated Transformation in Magnaporthe oryzae]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201903015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[Agrobacterium tumefaciens-mediated transformation (ATMT) is one of the most revolutionary techniques
in fungal research in recent 20 years. As a transformation tool, it has been widely used in the study of fungal gene
function, which made people have a better understanding of fungal genomics. Combined with phenotype screening of
mutants, random T-DNA obtained by ATMT was inserted into mutant library and the genetic basis of many processes
was elucidated. ATMT possesses the advantages of simple operation, wide transformation receptors, high transformation
efficiency and high stability of transformants etc.Magnaporthe oryzae, one of the top ten important fungi in plants, is
the pathogenic fungus that causes rice blast. At present, the most economical and environmentally-friendly method to
control rice blast is to cultivate rice resistant varieties. It is of great theoretical and practical significance to explain
the pathogenic mechanism and physiological characteristics and cultivate disease-resistant varieties by cloning the pathogenic genes related to Magnaporthe oryzae and studying their functions. ATMT provided an important tool for the study
of insertion mutation, gene knockout and marker cloning of Magnaporthe oryzae. The principle, characteristics, steps, and
influencing factors of ATMT and its application in functional genomics study of Magnaporthe oryzae were reviewed in order
to provide relevant references for the study of genetic transformation of fungi such as Magnaporthe oryzae etc.]]></description>
<pubDate>2019/4/24 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[何丽云 1
，张树林 2，崔百元 1
，朱庆锋 1
，刘圣杰 1
，刘文华]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>何丽云 1
，张树林 2，崔百元 1
，朱庆锋 1
，刘圣杰 1
，刘文华</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201903015&flag=1]]></guid><cfi:id>101</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Improvement of Physiological Control Methods of Adult Bradysia odoriphaga and Their Field Control Effects]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201903017&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The objective was to explore suitable methods to control, Bradysia odoriphaga Yang et
Zhang, a major pest that damage Chinese chives.【Methods】The oviposition taxis of adult B. odoriphaga to different
organic fertilizer was studied by selective experiment and the trapping and killing effect of mixture of sugar-acetic acidethanol (MSAE) with fermented manures were studied based on selective experiment. 40% phoxim EC, 5% chlorfluazuron
EC and yellow board were used as CK to explore the field control effects of the comprehensive application of decomposed
animal manure, MSAE, yellow board and biogas slurry on B. odoriphaga Yang et Zhang. 【Results】Cow dung, chicken
manure, sheep manure and mushroom residue had significant attraction to oviposition of adult B. odoriphaga Yang et
Zhang. The application of MSAE with cow dung and chicken manure could enhance the trapping and killing effect on adults. The biogas slurry-yellow board treatment, like drip irrigation with two chemical pesticides, showed rapidly available
effect on B. odoriphaga, and 9 days after the treatment, the plant protection rate was significantly higher than that of other
treatments.The control effects of MSAE-cow dung, yellow board, and yellow board-cow dung treatment were unremarkable
at the early stage, but gradually increased 36 days after treatment, and reached above 66% 63 days after treatment. The
protective effect of biogas slurry-cow dung treatment on Chinese chives was was comparable to drip irrigation of two
insecticides 9 d, 36 d and 63 d after treatment.【Conclusion】Adding in animal manure into MSAE could improve the
trapping effect of MSAE on adult B. odoriphaga . The control effect of the combined application of animal manure, MSAE,
yellow board and biogas slurry on adult B. odoriphaga was comparable to that of drip irrigation with recommend dosage of
Phoxim and Fluridin, therefore, it was worth recommending in the control of B. odoriphaga.]]></description>
<pubDate>2019/4/24 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[孙丽娟，张  芳，陈杰民，杨晓敏，康国栋，郑长英]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>孙丽娟，张  芳，陈杰民，杨晓敏，康国栋，郑长英</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201903017&flag=1]]></guid><cfi:id>100</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Screening and Identification of Biocontrol Bacteria Against Bacterial Wilt of Peanuts]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201902014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】To Screen the biocontrol bacterial strains against bacterial wilt of peanuts. 【Method】
The biocontrol bacterial strains which have antagonistic effects on bacterial wilt of peanuts were screened by plate 
confrontation method and the bacterial strains with good control effect were screened by pot experiment.【Result】Seven 
strains with antagonistic effects on bacterial wilt of peanuts were screened from over 300 bacteria strains isolated from 
rhizosphere soil. Among them, strains Ab, A27 and A38 had a bacterial inhibition diameter of more than 10 mm, and 
their secretions had an inhibitory effect on the bacteria. The three strains were further subjected to pot experiment, and 
the results showed that A38 got the best control effect, reaching 52.72%. The strain A38 was identified as Pseudomonas 
aeruginosa by physiological, biochemical and molecular biological identification.【Conclusion】A biocontrol bacterium 
with good antagonistic effect against bacterial wilt of peanuts was screened and identified as Pseudomonas aeruginosa.]]></description>
<pubDate>2019/3/26 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[陆  济，游春平，董章勇]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>陆  济，游春平，董章勇</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201902014&flag=1]]></guid><cfi:id>99</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Study on the Allelopathy Effects of Aqueous Extracts from Helianthus tuberosus on Echinochloa crusgalli (L.) Beauv.]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201902015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The allelopathic effects of aqueous extracts from different kinds of Helianthus tuberosus on 
barnyard grass were explored in order to reveal the mechanism of aqueous extracts from Helianthus tuberosus and provide 
a strong basis for their application in weed control.【Method】With the seeds of Echinochloa crusgalli (L.) Beauv. as 
experimental materials, a soil culture experiment was conducted to study the allelopathy of aqueous extracts from different 
organs of two H. tuberosus varieties from Xuzhou and Weifang on E. crusgalli. and to determine the root length, length of 
bud, germination rate, antioxidant enzyme activity, malondialdehyde (MDA) content of Echinochloa crusgalli (L.) Beauv, 
and the alkaline phosphatase, invertase activity and electronical conductivity(EC) of soil.【Result】With the increase of 
extract concentration, the germination rate of seeds decreased continuously, the highest inhibition rate reached 52.08%, 
and the maximum decrease of root length and length of bud reached 81.96% and 19.89%, respectively. The activity of 
POD, CAT and soil alkaline phosphatase increased first and then decreased with the increase of extract concentration 
and the SOD activity of E. crusgalli Beauv treated with aqueous extracts was generally inhibited. Aqueous extracts from 
Weifang H. tuberosus could induce MDA accumulation in E. crusgalli Beauv, while aqueous extracts from Xuzhou H. tuberosus made MDA content of E.crusgalli Beauv rise first and then drop. With the concentration of the aqueous extracts 
increased, the soil conductivity increased gradually and its maximum increase rate was 180.56%. The invertase activity 
showed a stimulating effect with the increase of the concentration of aqueous extracts from Weifang H. tuberosus.【Conclusion】
Aqueous extracts from H. tuberosus has certain effects on barnyard grass the growth, antioxidant capacity, MDA, and enzymes 
activity and EC in root soil of E. crusgalli Beauv. Therefore, aqueous extracts from H. tuberosus could be applied for weed 
control.]]></description>
<pubDate>2019/3/26 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[陈  瑞，张  琪，张婷婷，崔乐怡，杨  蓉，杨  蕾，余之琳，刘  鹏]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>陈  瑞，张  琪，张婷婷，崔乐怡，杨  蓉，杨  蕾，余之琳，刘  鹏</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201902015&flag=1]]></guid><cfi:id>98</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Infection Effect of A Strain of Aspergillus sp. Q527 onHeortiavites soides and Its Biological Characteristics]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201902016&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】In order to obtain more resources of insect-controlling fungi, the pathogenicity of an 
Aspergillus strain isolated from the dead larvae of Heortiavites soides in the field was determined and cultured to provide 
a theoretical basis for the cultivation of highly pathogenic biocontrol fungi.【Method】Aspergillus sp. Q527 was used as 
experimental material to determine the infection effect of Aspergillus sp. Q527 on H. soides by impregnation method. The 
culture conditions and sporulation characteristics of Aspergillus sp. Q527 were studied by mycelium growth rate method 
and sporulation quantity method.【Result】The results showed that the Aspergillus sp. Q527 had a good infection effect 
on the larvae of H. soides. The corrected infection rate was 83.3% after inoculation for 7 days, and the optimal medium for the growth of Aspergillus sp. Q527 was PDA medium. The average colony cultured on PDA medium was 78.18mm; the pH 
8 was suitable for growth and sporulation, with the size of 78.12mm and sporulation amount of 2.61×109; It could not resist 
high temperature and stopped growing and sporing germination at 65 C. Lactose, as a carbon source, effectively promoted the 
growth of Aspergillus sp. Q527 mycelium, with the size of 37.97mm; soluble starch, as a carbon source, effectively promoted 
Aspergillus sp. Q527 sporulation, with the sporulation amount of 2.03×109; peptone, as a nitrogen source, effectively 
promoted the growth and sporulation of Aspergillus sp. Q527 mycelium, with the size of 51.06 mm and the sporulation amount 
of 2.96×109 respectively; the best growth condition was in dark state with the colony of 78.17mm and the sporulation amount 
of 2.63×109.【Conclusion】Aspergillus nomius Q527 is lethal, fast-growing and adaptable to H. soides, and has potential 
application value in biological control of H. soides.]]></description>
<pubDate>2019/3/26 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[赵鹏飞 1，2，常明山1，2，罗　辑 2­，吴耀军 2，杨　娟 3，杨振德 1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>赵鹏飞 1，2，常明山1，2，罗　辑 2­，吴耀军 2，杨　娟 3，杨振德 1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201902016&flag=1]]></guid><cfi:id>97</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effect of Candidatus Liberibacter asiaticus Infection on the Activities of SOD and POD in Catharanthus roseus]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201901011&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[：【Objective】The objective is to compare the effects of Candidatus Liberibacter asiaticus(CaLas) 
infection on the activity of superoxide dismutase (SOD) and peroxidase (POD) in three organs, i.e. leaves, stems and roots of 
Catharanthus roseus. 【Method】The activities of SODand POD in the leaves, stems and roots of C. roseus infected by CaLas 
were determined by spectrophotometer method.【Result】The activity of SOD in the affected C. roseus decreased gradually 
with the increase of infection duration. When the activity was the lowest, the activity of SOD in the leaves, stems and roots 
was only 61%, 55% and 47% respectively of that of the healthy control group. Among these three organs, the leaves had the 
highest SOD activity while the roots had the lowest. The activity of POD in the affected C. roseus increased first and then 
decreased with the increase of infection duration. The activity of POD in leaves, stems and roots reached the highest 30, 35 
and 35 days after grafting, and was 205%, 254% and 194% respectively of that of the healthy control group. The activity of 
POD in roots was much higher than that in leaves and stems【Conclusion】The infection of CaLas has a great effect on the 
activity of SOD and POD in the organs of C. roseus. The increase of SOD and POD activities plays an important role in the 
resistance of C. roseus to CaLas infection.]]></description>
<pubDate>2019/3/12 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[李  亚 1，2，余沁涵 1，鲍敏丽 2，陈燕玲 2，邓晓玲 2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>李  亚 1，2，余沁涵 1，鲍敏丽 2，陈燕玲 2，邓晓玲 2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201901011&flag=1]]></guid><cfi:id>96</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effects of Fungicides on Fruit Diseases and Pre-harvest Fruit Drops of Chestnuts in Long'an County]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201901012&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The objective is to identify the cause of fruit diseases and pre-harvest fruit drop of Chinese 
chestnuts in Long’an and select the effective fungicides for the diseases and drop in order to provide a scientific basis for 
the prevention of fruit diseases and pre-harvest fruit drop of chestnuts.【Method】Through field investigation of 14-yearold chestnuts, the effects of different fungicides on fruit diseases and pre-harvest fruit drop of chestnuts in Long’an 
County were carried out in response to the phenomenon of fruit drop and diseases of chestnuts. Clear water was used in the 
control group. Five fungicides, including Carbendazim, Kasugamycin, Diphenoxazole, Kasugamycin + Diphenoxazole and 
Thiodiazole-copper were sprayed, individually or in combination, on the chestnuts for 3 times one month before harvest, 
with the last spraying performed about 15 days before harvest. And the rates of pre-harvest fruit drop and diseases were 
investigated.【Result】The five fungicides, sprayed individually or in combination, could reduce the fruit drop before 
harvest. The average rate of fruit drop was between 10.60% and 34.24%, with the best control effect found in carbendazim 
(10.60%). In addition, the rate of fruit diseases ranged from19.69% and 33.49%, with an overall average of 27.16%. 
The average rate of fruit drop was 19.69% for those sprayed with carbendazim. Three fungi strains that caused the fruit diseases of chestnuts were finally identified through a pathogenic bacteria separation, purification and pathogenicity 
test.【Conclusion】For the fruit protection measures of chestnuts, in-depth research should mainly focus on the rational 
spraying and scientific management of the fungicide carbendazim to reduce the fruit drop and diseases and improve economic 
benefits and yield.]]></description>
<pubDate>2019/3/12 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[孙万霞，潘晓芳]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>孙万霞，潘晓芳</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201901012&flag=1]]></guid><cfi:id>95</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Phototoxicity of Two Paddy Herbicides on Tobacco and Screening of Alternative Herbicides]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201901013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】To choose the safe herbicides, the field experiments were carried out.【Method】The pot 
experiment were conducted to study the influence on the growth of tobacco caused by two paddy herbicides. 【Result】The 
results indicated that Quinclorac and Bensulfuron-methyl could affected the height, leaf length and leaf width of tobacco. 
Quinclorac had significant effect on leaf width and made the blades to be narrow and linear with no commercial value. The 
inhibition rate of leaf width was up to 10% when the concentration of quinclorac in soil was 2×10-4 mg/kg. Moreover, the 
influence of Bensulfuron-methyl mainly included that plant dwarfed leaf number decreased and production dropped. The 
inhibition rate on plant height was more than 10% in case of 2×10-3 mg/kg Bensulfuron-methyl concentration in soil. 
【Conclusion】The result showed that Benzoicacid could be used as alternation agent to control broadleaf weeds instead of 
Bensulfuron-methyl. Butachlor could control gramineous weeds instead of Quinclorac. Fluoroglycofen-ethyl could control 
gramineous weeds and broadleaf weeds instead of Quinclorac and Bensulfuron-methy. Thus, three herbicides (Benzoicacid, 
Butachlor, Fluoroglycofen-ethyl) would be used in rice filed safely and no influence on stubble crop tobacco.]]></description>
<pubDate>2019/3/12 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[沈会芳，蒲小明，张景欣，孙大元，林壁润]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>沈会芳，蒲小明，张景欣，孙大元，林壁润</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201901013&flag=1]]></guid><cfi:id>94</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Investigation on the Pseudo-rust Disease of Pueraria lobata in Guangxi Province]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201912011&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The pseudo-rust disease caused by Sychytrium puerariae Miy infected the leaves, petioles 
and stems of Pueraria lobata, resulting in malformation of these tissues and slow growth of stems and tuberous roots, 
which severely affected the yield P. lobata. The investigation was conducted to understand the occurrence of pseudo-rust 
in P. lobata producing areas of Guangxi and find out effective control measures.【Method】The occurrence of pseudorust of four P. lobatas varieties bred by the research group was investigated in the promotion areas of Guangxi in 2018—
2019.【Result】The results showed that the disease index (ID) of different Pueraria varieties in the producing areas 
was between 0-50.31, and there were great differences among varieties. The large leaf medicinal type Guige 18 was a 
high resistant variety, with ID of 0-0.16 and average ID of 0.04. The large leaf processing type Guige 8 was a moderately 
susceptible variety, with ID of 18.00-50.31 and average ID of 31.98. Both of the large leaf processing type Guige 17 and 
small leaf vegetable type Guige 1 were moderately resistant varieties, with IDs of 0.10-30.79 and 6.20-30.83 and average 
IDs of 16.48 and 16.77, respectively.【Conclusion】The disease severity of the Guige 18 was significantly lower than that 
of the Guige 8. And the disease severity of issue culture virus-free seedlings of the same variety was significantly lower 
than that of cutting seedlings.]]></description>
<pubDate>2020/3/4 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[潘睿扬，袁高庆，何龙飞，肖　冬，王爱勤]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>潘睿扬，袁高庆，何龙飞，肖　冬，王爱勤</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201912011&flag=1]]></guid><cfi:id>93</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Determination of Indoor Toxicity of Azadirachtin to Diaphorina citri and its Control Effect in Field]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201912012&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The research was to evaluate the indoor toxicity and field control effect of azadirachtin to 
Diaphorina citri and provide a scientific basis for the biocontrol of D. citri. 【Method】The indoor toxicity and field control 
effects of azadirachtin on the 3rd instar nymphs and adults of D. citri were determined by leaf soaking method and spraying 
method. 【Result】Under indoor condition, the LC50 values of 3rd instar nymphs were 2.77, 1.14 and 0.61 mg/L, respectively 
after being treated with azadirachtin for 1 d, 3 d and 7 d. However, the mortality rate of adult D. citri was only 13.33% after 
being treated with 16.0 mg/L azadiracthin for 4d, which had no significant difference with the water control. The field control 
effects on D. citri nymphs were 14.35%, 49.56% and 86.4%, respectively after sprayed with 800 times diluent of 0.3% 
azadirachtin EC for 1 d, 3 d and 7 d.【Conclusion】Azadirachtin had good toxicity activity against D. citri nymphs and 
showed the best field control effect on D. citri nymphs after being treated for seven days, while it had no toxicity activity toadults. Azadirachtin has a favorable application prospect in the control of D. citri nymphs.]]></description>
<pubDate>2020/3/4 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[赖　多1
，曹　旭 2，邵雪花 1
，肖维强 1
，刘传和 1
，贺　涵 1
，刘　秀 2，匡石滋 1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>赖　多1
，曹　旭 2，邵雪花 1
，肖维强 1
，刘传和 1
，贺　涵 1
，刘　秀 2，匡石滋 1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201912012&flag=1]]></guid><cfi:id>92</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effective Inhibition Duration and Field Control Effectsof 16 Fungicides Against Neoscytalidium dimidiatum]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201912013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Fungicides with long effective inhibition duration and good field control effects against 
Neoscytalidium dimidiatum were screened to provide scientific basis for the control of the most destructive pitaya brown 
rot disease in pitaya production.【Method】By measuring the size and disappearing time of inhibition zone, the effective 
duration of 16 fungicides against N. dimidiatum mycelial growth were determined by agar diffusion method. And field control 
efficacy of fungicides with good effective inhibition or long duration were tested and compared.【Result】Six fungicides,including fludioxonil, propiconazole, prothioconazole, tebuconazole, flusilazole and iprodione, showed long effective inhibition 
duration, and the inhibition zone did not disappear after being cultured for 15 days. Three fungicides, including prochloraz, 
difenoconazole and myclobutanil, showed relatively long effective inhibition duration. Three fungicides, including carbendazim, 
probenazole and azoxystrobin, showed short effective inhibition duration. Four fungicides, including propineb, kasugamycin, 
chlorothalonil and epoxiconazole, showed extremely short effective inhibition duration. Field efficacy tests indicated that 5 
fungicides, including tebuconazole, iprodione, lusilazole, prothiazolium and propiazole, showed good control effects on the 
tender stem of the pitaya. Except that procineazole at the active ingredient concentration of 500 mg/kg could inhibit the growth of 
tender stems of pitaya, the other 4 fungicides were safe for the growth of pitaya.【Conclusion】It is preferentially recommended 
to use systemic fungicides of tebuconazole, iprodione, lusilazole, prothiazolium and propiazole to control the pitaya brown rot 
disease with rotation, and application concentration of which should be appropriately increased while the pitaya orchard facing 
with high disease pressure.]]></description>
<pubDate>2020/3/4 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[蓝国兵 1, 2，何自福 1
，于　琳 1
，汤亚飞 1
，李正刚 1
，邓铭光 1
，佘小漫 1, 2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>蓝国兵 1, 2，何自福 1
，于　琳 1
，汤亚飞 1
，李正刚 1
，邓铭光 1
，佘小漫 1, 2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201912013&flag=1]]></guid><cfi:id>91</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Isolation and Biological Characteristics of Tobacco Ralstonia solanacearum Bacteriophage]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201911011&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Tobacco bacterial wilt a widespread and devastating bacterial disease in southern tobacco 
production area of China, and there is no effective chemical for the control of such disease. The screening of phage will 
provide phage resources for the prevention and control of tobacco bacterial wilt in the future.【Method】Soil samples were 
collected from the tobacco field of Ji'an City, Jiangxi Province, and the bacterial wilt phages were isolated by double-layer plate 
culture and isolation method and the effects of physical and chemical factors on the activity of phage were further compared.
【Result】Six Ralstonia solanacearum bacteriophages were isolated from the soil of tobacco field in Ji'an City, and numbered 
as PTb7-1，PTb1521，PTb1553，P1Tb1556，P2Tb1556 and PTb574，among which there were differences in biological 
characteristics. The results of biological characteristics research showed that the optimum growth temperature for several 
phages was about 35℃ , the lethal temperature was 55-60℃ and the optimum pH was 5-9, and those phages were sensitiveto ultraviolet, ether and chloroform. However, phage PTb7-1 was only suitable to grow and propagate under acidic environment. 
Phage P2Tb1556 was not sensitive to ultraviolet and ether, but highly sensitive to chloroform.【Conclusion】There are abundant 
phage resources in tobacco soil, which is a good source for phage screening. The biological characteristics of the six phages is 
different. P2Tb1556 phage is not sensitive to ultraviolet light and ether, but sensitive to chloroform.]]></description>
<pubDate>2020/1/10 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[胡蓉花 1
，余成鹏 2，任敏华 2，李小勇3，刘琼光 2，4]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>胡蓉花 1
，余成鹏 2，任敏华 2，李小勇3，刘琼光 2，4</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201911011&flag=1]]></guid><cfi:id>90</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Advances in Research of Post-harvestPineapple Blackheart Disease]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201911012&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[Pineapple blackheart disease is a physiological disease that is easy to occur in the process of post-harvest storage 
and transportation of pineapple. Its shelf life is only 6-8 d, which seriously restricts the development of pineapple industry and 
import-export trade. In this paper, the research status of pineapple blackheart disease in Bali in recent years is summarized from 
three aspects: the pathogenesis, the pre-and post-harvest control methods, and the existing problems and the prospect. A series 
of complex physiological and biochemical reaction processes are involved after the occurrence of pineapple blackheart disease. 
The pathogenesis can be studied from the aspects of enzymatic browning, membrane lipid metabolism, sugar and acid metabolism, 
active oxygen accumulation and etc. The control methods mainly include: cultivating Bali fruit in summer and resistant varieties 
in winter, controlling the harvest time of Bali pineapple, air-conditioned preservation combined with low-temperature storage. 
Comprehensive prevention and control of pineapple blackheart disease is required. A series of measures, such as improving 
fruit quality, selecting resistant varieties and optimizing pre- and post-harvest treatments are taken to reduce the occurrence of 
pineapple blackheart disease. The fundamental control of pineapple black heart disease lies in the improvement of varieties. With 
the progress of gene editing technology and the maturity of pineapple genetic transformation system, Bali pineapple blackheart 
disease will be eliminated fundamentally, which will greatly promote the development of pineapple industry in Leizhou Peninsula.]]></description>
<pubDate>2020/1/10 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[宋康华，谷　会，张鲁斌，洪克前，贾志伟，侯晓婉]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>宋康华，谷　会，张鲁斌，洪克前，贾志伟，侯晓婉</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201911012&flag=1]]></guid><cfi:id>89</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Pathogenic Identification of Citrus Melanose andIndoor Screening of Fungicides]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201911013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】In recent years, citrus melanose has been widespread in citrus planting areas, which has 
a great impact on the citrus production. This paper aims to screen out new fungicides with strong antifungal effect on the 
pathogen of citrus melanose, which can provide a reference for selecting more rotating fungicides to control the disease 
in the field.【Method】The pathogen was isolated from symptomatic citrus fruits collected in Guangxi and identified 
based on the morphological characteristics and rDNA-ITS sequence analysis. Antifungal effects of 21 fungicides on the 
pathogen were tested by mycelial growth inhibition method, and fungicides with good effects were selected to determine 
their toxicity to the pathogen.【Result】Three strains with morphological characteristics of Diaporthe citri were isolated 
from the diseased fruits, and the homology of rDNA-ITS sequence of the strain and that of D. citri in GenBank was 
99%, therefore, the pathogen of citrus melanose in Guangxi was identified as D. citri, Pyraclostrobin·cuppric nonyl 
phenolsulfonate, epoxiconazole·thifluzamide, copper quinolate·pyraclostrobin, epoxiconazole·pyraclostrobin and picoxystrobin 
were selected from 21 kinds of fungicides for toxicity test. The EC50 values of five fungicides were 0.106 μg/mL, 0.814 μg/mL, 
0.064 μg/mL, 0.053 μg/mL and 0.056 μg/mL, respectively, and fepoxiconazole·pyraclostrobin had the highesttoxicity to D. citri. 【Conclusion】Pyraclostrobin·cuppric nonyl phenolsulfonate, epoxiconazole·thifluzamide, copper 
quinolate·pyraclostrobin, epoxiconazole·pyraclostrobin and picoxystrobin had strong inhibitory effect on D. citri.]]></description>
<pubDate>2020/1/10 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[何永林 1
，黄晓琴 2，黎起秦 1
，陆　温 1
，林　纬 1
，袁高庆 1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>何永林 1
，黄晓琴 2，黎起秦 1
，陆　温 1
，林　纬 1
，袁高庆 1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201911013&flag=1]]></guid><cfi:id>88</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Study on the Parasitic Characteristics ofDiachasmimorpha longicaudata (Ashmead) onDifferent Insecticide-resistant Strain Hosts]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201911014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was to clarify the selection tropism of Diachasmimorpha longicaudata on 
Bactrocera dorsalis Hendel with different resistance levels and the effects of insecticide-resistant host stains on the 
development characteristics of D. longicaudata.【Method】By means of“Y”-tube olfactometer testing and choice 
test, the behaviour response and parasitic capacity of D. longicaudata to three kinds of B. dorsalis Hendel insecticideresistant strains were determined, and the larval development of D. longicaudata on these three strain hosts werealso investigated by biological observation test.【Result】The results showed that D. longicaudata had no significant 
selection tropism to B. dorsalis Hendel strains that resistant to trichlorphon, beta-cypermethrin and abamectin. Similarly, 
there was no significant difference in the parasitic number of D. longicaudata to different B. dorsalis Hendel resistant 
strains. The D. longicaudata on different resistant host strains showed sex differences in larval developmental duration, 
viz., there was no significant difference in the larval development duration of female wasps on different test host strains, 
while the developmental duration of male wasps on high resistant strains was significantly shorter than that on middle 
resistant strains and regular strains.【Conclusion】Different insecticide-resistant strains of oriental fruit fly had no 
significant effect on the parasitic rate of D. longicaudata, but showed significant influence on the developmental duration 
of male wasps. The test result can provide a theoretical basis for the effective control of oriental fruit fly by the use of the D. 
longicaudata.]]></description>
<pubDate>2020/1/10 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[赵海燕 1
，吉训聪 1
，陈海燕 1
，韦德卫 2，林珠凤 1
，刘吉敏 2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>赵海燕 1
，吉训聪 1
，陈海燕 1
，韦德卫 2，林珠凤 1
，刘吉敏 2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201911014&flag=1]]></guid><cfi:id>87</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Research on Identification and Biological Characteristicsof the Pathogen of Philodendron bipinnatifidum]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201910012&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】A new leaf spot disease was found from Philodendron bipinnatifi dum in Wenchang, Hainan 
Province and its pathogen and biological characteristics were identified. 【Method】Isolation culture and inoculation 
test were carried out, and traditional morphology and molecular methods was adopted identify the pathogen. The crossing 
method and the blood cell count plate method were used to determined its biological characters. 【Result】The pathogen 
was identified as by Colletotrichum gloeosporioides. The colony of the pathogen was white at the initial stage and then 
changed to green-grey with red spore piles. The conidia were ellipse with the mean size of 6.14~14.69 μm × 2.96~6.49 
μm. The optimum temperature for the colony growth was 25℃ . The optimum pH value was 9. In the tested carbon sources, 
the pathogen could make use of many kinds of carbon sources, but the effect was different. The optimum carbon source and 
nitrogen source were sucrose and sodium nitrate, respectively. Obviously, ammonium nitrate and ammonium sulfate(as nitrogen 
sources) were not conducive for the mycelium growth. 【Conclusion】The pathogen of anthracnose in P. bipinnatifi dum was 
isolated and identified for the first time. The optimum growth temperature and pH for the growth of the pathogen were 25℃ 
and 9, respectively. The optimum carbon and nitrogen sources were sucrose and sodium nitrate.]]></description>
<pubDate>2019/12/17 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[余凤玉，宋薇薇，韩　轩]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>余凤玉，宋薇薇，韩　轩</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201910012&flag=1]]></guid><cfi:id>86</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Research on Cold Storage Technology for Eggs of Hermetia illucens L．]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201910013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The egg production of Hermetia illucens L.is greatly affected by continuous low temperature 
or rainy weather. It is helpful to standardize the production of H. illucens by refrigerating the surplus eggs during the peak 
spawning period.【Method】In this paper, the egg hatching rate of H. illucens at different developmental stages after being 
refrigerated at 12℃ for different days, and the hatching, growth and development of eggs at gastrula stage at 32℃ after 
being refrigerated at 12℃ for 5, 10 and 15 days respectively was studied. 【Result】The results showed that the primiparous 
eggs could not be refrigerated directly. Refrigerated at 12℃ for 1 day and then incubated at 32℃ , the mortality rate of 
eggs was 100%. The eggs at blastoderm and germ band formation stage, gastrula stage, dorsal closure stage and hatching 
stage were refrigerated at 12℃ and then incubated at 32℃ . The average hatching rate were 94%, 94%, 27% and 0%, 
respectively. Therefore, the eggs at dorsal closure stage and hatching stage were not suitable for cold storage. The hatching 
rate of eggs during blastoderm and germ band formation stage was reduced to less than 80% after 13 days of cold storage. 
The hatching rate of the eggs during gastrula stage was reduced to less than 80% after 15 days of cold storage. There were 
no significant difference in the growth and development between the larvae hatched from the eggs at gastrula stage that 
were refrigerated for 5 and 10 days and the larvae of control group. The survival rate of the eggs at gastrula stage which were refrigerated for 15 days was lower than that of the control group. There were no significant difference in the weight of 
one hundred larvae. 【Conclusion】The eggs at gastrula stage refrigerated at 12℃ can maintain a high survival rate, and the 
larvae can grow and develop normally. The results can be used to provide theoretical basis and practical guidance for industrial 
production of H. illucens.]]></description>
<pubDate>2019/12/17 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[王凤英 1，2 ，肖乾鹏 1
，王　星 2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>王凤英 1，2 ，肖乾鹏 1
，王　星 2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201910013&flag=1]]></guid><cfi:id>85</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Influence of Pesticide Adjuvants on the Prevention and Control of Bemisia tabaci in Cucurbita pepo L. with Acetamiprid]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201910014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The tests were conducted to evaluate the synergistic effects of four kinds of additives, namely, 
organosilicon, mixed orange essential oil, green peel orange oil and Jijian, on field control efficacy of acetamiprid against 
Bemisia tabaci, and to evaluate the decrement effects of 3000-fold dilution of organosilicon on field control efficacy of 
acetamiprid against B. tabaci. 【Method】 The field efficacy trials were conducted by spraying .The recommended dose of 5% 
acetamiprid EC was mixed with organosilicon 3000-fold dilution, orange essential oil 1000-fold dilution, green peel orange 
oil 1000-fold dilution and Jijian 3000-fold dilution, respectively, to determine the control efficacy of droplets density and 
coverage rate of acetamiprid on B. tabaci and the decrement effects of 3000-fold dilution of organosilicon on field control 
efficacy of acetamiprid against B. tabaci.【Result】The results showed that the recommended dose of 5% acetamiprid 
EC mixed with additives could increase the droplets of the liquid. However, the recommended dose of 5% acetamiprid EC 
mixed with different additives showed different control effects to B. tabaci. Among the tested additives, the organosilicon had 
obvious synergistic effect and long persistent on acetamiprid against B. tabaci, followed by green peel orange oil, and there 
was no significant difference between the mixed solution of orange essential oil and acetamiprid EC and branch water. 5% acetamiprid EC mixed with organosilicon could improve the control effect of B. tabaci, and reduce the application amount of 
10%-40%.【Conclusion】5% acetamiprid EC mixed with organosilicon 3000-fold dilution can be used for the prevention and 
control of B. tabaci in practice.]]></description>
<pubDate>2019/12/17 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[吴颖仪 1，2，冯伟明 1
，郝东川1
，陈育民 1
，黎　昆 1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>吴颖仪 1，2，冯伟明 1
，郝东川1
，陈育民 1
，黎　昆 1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201910014&flag=1]]></guid><cfi:id>84</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Preliminary Study on the Mechanism of a Strain Bacillus licheniformis HS10 against Cucumber Downy Mildew]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202009011&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Bacillus licheniformis HS10 is a biocontrol strain obtained by a series of screening in the previous study. And the disease defense spetrum and mechanism of such strain were observed in this study.【Method】By comparing the pathogens with similar classification status with cucumber downy mildew, the litchi downy mildew, caused by Peronophythora litchii Chen ex Ko et al., was selected as control object. The litchi fruits after-harvest and in the field were pre-treated to observe the control effect of litchi downy mildew. A primary exploration on the disease defense mechanism was conducted by analyzing the crude protein produced by the strain HS10 and the plant disease-resistant enzyme activity.【Result】The HS10 strain had a control efficacy of 62.36% against post-harvest litchi downy mildew and a field control efficacy of 46.00%. The control efficacy of final concentration of 50 g/mL crude protein against cucumber downy mildew in the greenhouse was 60.08%. Extracellular crude protein could inhibit the growth of Phytophthora capsici, causing abnormal growth of the hypha tip, expansion and distortion. After the cucumber plants being treated with HS10 strain, PAL and POD increased in the early stage, then decreased, and increased rapidly in the late stage.【Conclusion】B. licheniformis HS10 also had control efficacy on other similar diseases. The strain inhibited the growth of hyphae through exocytic protein and affected relevant plant defense enzyme activity, which laid a foundation for further exploration of control mechanism.]]></description>
<pubDate>2020/11/7 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[ZHENG Li1,2, XU Long4, LUO Yuming3, LIU Hongxia2, GUO Jianhua2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>ZHENG Li1,2, XU Long4, LUO Yuming3, LIU Hongxia2, GUO Jianhua2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202009011&flag=1]]></guid><cfi:id>83</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effect of Tea Leafhopper on the Biochemical Components of Tea and Control Effect of High-efficiency Low Water-soluble Pesticides]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202009012&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The control effects of three low water-soluble pesticides on tea leafhoppers were tested, and the effects of tea leafhoppers on the biochemical components of tea during different damage periods were analyzed so as to provide a basis for taking strong picking measures in a timely manner.【Method】Taking strong picking and no control as control, three kinds of low water-soluble pesticides of 24% chlorfenapyr, 15% indoxacarb, and 22% thiamethoxam+lambdacyhalothrin were selected and three application concentrations were designed to test their control effects on tea leafhoppers. And the young shoots of damaged tea leaves were picked to analyze the change of their main biochemical contents.【Result】There were differences in the main biochemical components of normal tea and the tea damaged by tea leafhoppers. The contents of tea 
polyphenols, caffeine, and soluble sugar showed an upward trend and then a downward trend. The contents of amino acids and moisture showed a downward trend, of which the content of amino acids decreased by 22.7%, reaching significant difference. With the increase in the level of damage, the contents of tea polyphenols, amino acids, caffeine, soluble sugar, and moisture all showed a downward trend. After treatment for 1d, the effects of different treatments （from high to low） showed as: 24% chlorfenapyr>strong picking>22% thiamethoxam+lambda-cyhalothrin>15% indoxacarb, where the difference in the efficacy of 24% chlorfenapyr reached a significant level, and the effect was greater than 85%. The prevention and treatment effects of 15% indoxacarb and 22% thiamethoxam+lambda-cyhalothrin showed a trend of decreasing first and then increasing after treatment for 1 d. The lowest peaks reached at the 7th day （48.73%） and the 3rd day （26.19%） after drug application, and the highest peaks reached at the 21st day （90.74%, 83.80%） after drug application. The occurance number of tea leafhoppers showed a significantly negative correlation with picking frequency.【Conclusion】When the damage level of tea is Grade 1, it is better to adopt strong picking measures for prevention and control. The water loss of new shoots is light, and the phenol-ammonia ratio is relatively low. It is suitable to pick and produce green tea; When the damage level is Grade 2-3, it is suggested to take strong picking and 24% chlorfenapyr, and leaf quality of young shoots is crisp with high phenol-ammonia ratio, which is suitable for the production of black tea.]]></description>
<pubDate>2020/11/7 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[LIU Jiayu1, LI Jianlong1, ZHOU Bo1, CHEN Yiyong1, LIAO Zhencheng2, TANG Hao1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>LIU Jiayu1, LI Jianlong1, ZHOU Bo1, CHEN Yiyong1, LIAO Zhencheng2, TANG Hao1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202009012&flag=1]]></guid><cfi:id>82</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Advances in Molecular Research of Sterile Insects]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202009013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[Sterility is an effective means applied to pest control. Sterile insect techniques （SIT）, as an Area-Wide Integrated Pest Management （AW-IPM） measure, can block the invasion of alien species and the damage of agricultural pests to the greatest extent, while the rapid development of biomolecule technology has also contributed to many innovative achievements in SIT research. In this paper, various novel sterility techniques and ideas are described, and their advantages and limitations are summarized and evaluated, providing references for the rational use of different techniques. With SIT as an introduction, the advances in ideas of studies on applications of various molecular genetic control mechanism are summarized, and unique thoughts are made on the current sterile techniques, and corresponding suggestions are made for their large-scale practical applications. The use of sterile insects should be efficient and concise, and a large number of strains with low irradiation tolerance genes should be released in the wild so that the trait can be spread in the wild population. The insect population can be controlled by the use of ultra-low doses of irradiation.]]></description>
<pubDate>2020/11/7 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[YANG Yuhang, HUANG Xinyi, WENG Qunfang]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>YANG Yuhang, HUANG Xinyi, WENG Qunfang</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202009013&flag=1]]></guid><cfi:id>81</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Study on the Causes and Control Techniques of Rice Straight Head in Mianyang City]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202008011&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was to explore the causes of rice straight head in Mianyang City and the corresponding control measures.【Method】By means of investigation and field test, the incidences of rice straight head under different herbicide treatments were compared, and the types and contents of heavy metals in soil and rice plant were measured to preliminarily determine the causes of rice straight head in Mianyang City. The control measures were explored by analyzing the incidences of rice straight head under different control methods.【Result】Herbicide application was not the cause of rice straight head, but excessive herbicide application could significantly reduce rice yield. The content of As in the soil layer of 0-50 cm in diseased field was 2.3-5.6 mg/kg higher than that in non-diseased field. The content of As in diseased plants was 0.03-0.20 mg/kg higher than that in non-diseased plants. Compared with the control, sulphur application during wheat season and lime application during rice season could reduce the incidence rate of rice straight head by 20.74%. The planting of japonica rice varieties in the fields could significantly reduce the incidence rate of susceptible rice straight head compared with that of indica rice varieties.【Conclusion】The occurrence of rice straight head in Mianyang City was related to the high content of As, Cd and Cu in soil. The four herbicides, bensulfuron-methyl and acetochlor, pyrazosulfuron, quinclorac and penoxsulam, were not the causes of rice straight head. Under the conditions of wheat-rice rotation and full straw returning, sulfur application in wheat season and lime application in rice season can prevent and control the disease effectively.]]></description>
<pubDate>2020/9/27 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[LIU Qian1，WANG Xuechun1，LUO Huayou3，SONG Xiang2，LAI Peng1，WANG Xiaosong2，HU Yungao1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>LIU Qian1，WANG Xuechun1，LUO Huayou3，SONG Xiang2，LAI Peng1，WANG Xiaosong2，HU Yungao1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202008011&flag=1]]></guid><cfi:id>80</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Changes of Enzyme Activities in Resistant and Susceptible Near-isogenic Lines of Rice after Infection with Xanthomonas oryzae pv.oryzicola]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202008012&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was conducted to explore the changes of enzyme activity in rice resistant and susceptible near-isogenic lines infected with Xanthomonas oryzae pv.oryzicola（Xooc）, which provided a theoretical basis for studying the resistance mechanism of rice bacterial leaf streak（BLS）.【Method】With the rice BLS resistant near-isogenic line LR19 and susceptible near-isogenic line LS19 as materials, the treatment group was inoculated with pathogenic bacteria of Xooc by needling method, while the control group was inoculated with sterile water. The leaves at tillering stage of rice were taken as samples at 0, 24, 48 and 96 h after inoculation, and the changes of enzyme activities of 
malondialdehyde（MDA）, catalase（CAT）, phenylalanine ammonia-lyase（PAL）, and peroxide enzyme（POD）, polyphenol oxidase（PPO）and superoxide dismutase（SOD）were analyzed.【Result】After inoculation, the average activities of CAT, PAL, POD, PPO and SOD in the LR19 treatment group were 269.36, 39.61, 31 677.75, 106.4 and 2 035.44 U/g, respectively, and those in the control group were 195.16, 33.23, 21 449.75, 85.67 and 1 139.38 U/g.The average activities of CAT, PAL, POD, PPO and SOD in the LS19 treatment group were 149.13, 34.3, 26 635.25, 87.6 and 1 354.82 U/g, respectively, and those in the control group were 73.95, 30.45, 10 506.43, 62.3 and 779.5 U/g, respectively. After being infected with Xooc, the activities of CAT, PAL, POD, PPO and SOD in both near-isogenic lines increased, and the activities of these five enzymes in LR19 were higher than those in LS19. However, the infection of Xooc reduced the MDA content in LR19 and LS19, and the MDA content of LR19 was generally lower than that of LS19.【Conclusion】The enhanced activity of CAT, PAL, POD, PPO and SOD is conducive to improving the resistance to BLS, while the accumulation of MDA content is negatively correlated to the BLS resistance.The activities of these enzymes can be used as supplementary evaluation indexes for identification of resistance to rice BLS.]]></description>
<pubDate>2020/9/27 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[HE Shengxian1，2，WAN Yao1，ZHANG Hui1，LIU Fang1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>HE Shengxian1，2，WAN Yao1，ZHANG Hui1，LIU Fang1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202008012&flag=1]]></guid><cfi:id>79</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effects of Thidiazuron 0.2% SL on Yield and Quality of Mango]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202008013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Mango（Mangifera indica L.）enjoys the reputation of " King of Tropical Fruit " and is one of the most important tropical fruits in China. As a new and highly effective exogenous plant growth regulator, Thidiazuron（TDZ）is widely used in agricultural production. This paper aims to study the effects of thidiazuron 0.2% SL on the yield and fruit quality of mango.【Method】Five different concentrations of thidiazuron 0.2% SL were sprayed at the young fruit and fruit expansion stages of the mango to assess their effects on the production, quality and relevant indicators of mango. 【Result】Compared with the blank control, thidiazuron 0.2% SL could significantly increase the single plant yield of mango by 44.92%-55.60%, with the fruit quality increased by 43.63%-53.04%, the fruit transverse diameter increased by 7.52%-9.83%, the fruit longitudinal diameter increased by 27.53%-33.28%, the soluble content of mango pulp increased by 5.66%-8.12%, the content of vitamin C increased by 10.66%-14.04%, the sugar content increased by 6.71%-14.20%, while the titratable acid content decreased by 10.39%-18.42%.Compared with the control agent 0.003% propionyl brassinolide aqueous solution（CK）, the fruit yield, quality and fruit shape index under thidiazuron 0.2% SL treatment were significantly higher than those of the control agent, and there was no significant difference in quality indicators, all of which far exceeded the standards of Ministry of Agriculture and Rural Affairs standard for superior fresh mango.【Conclusion】Thidiazuron 0.2% SL significantly improved the yield and quality of mango. At the dosage of 3.33 mg/kg, the indicators of mango reached a high level, and showed no significant difference to the maximum value, therefore, the recommended concentration of thidiazuron 0.2% SL for mango production was 2.50-3.33 mg/kg.]]></description>
<pubDate>2020/9/27 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[TIAN Qingheng1, LI Zhaoyang2, TANG Liangde3, WU Jianhui2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>TIAN Qingheng1, LI Zhaoyang2, TANG Liangde3, WU Jianhui2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202008013&flag=1]]></guid><cfi:id>78</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Study on the Synergism of Two Adjuvants on Bromadiolone Baits]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202008014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】In order to increase the utilization rates and prevention and control effects of anticoagulant rodenticides and reduce their ecological risks, the synergistic effects of two adjuvants on bromadiolone baits were investigated.【Method】The content distribution of bromadiolone in the poison grains prepared by different varieties and concentrations of adjuvants were determined by using high-performance liquid chromatography. The palatability and the toxic effects of the bromodiolone poison grains prepared with different adjuvants for mice were evaluated by selective and non-selective feeding tests.【Result】The content of bromadiolone in the brown rice of 0.01% poison grains treated with 4% N, N-dimethylformamide and 4% dimethyl sulfoxide was significantly higher than that in the 0.01% poison grains, with the increase of 271.17% and 163.51%, respectively. While the content of bromadiolone in the brown rice of 0.0025% poison grains treated with 4% N, N-dimethylformamide and 4% dimethyl sulfoxide was significantly higher than that in the 0.0025% poison grains, with the increase of 433.33% and 400.95%, respectively. The results of bioassay showed that the 0.0025% bromodiolone poison grains with two kinds of adjuvants exhibited good palatability and potent efficacy on mice. Their feeding coefficient were 2.69 and 0.80, with the poison rates of 100% and 90%, which were significantly higher than the poison rate of the 0.0025% bromodiolone poison grains, but there was no significant difference with the 0.01% bromodiolone poison grains.【Conclusion】4% N, N-dimethylformamide and 4% dimethyl sulfoxide showed significant synergistic effects to the bromodiolone poison baits, and decreased the use concentration of rodenticide obviously.]]></description>
<pubDate>2020/9/27 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[JIANG Hongxue, YAO Dandan, FENG Zhiyong]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>JIANG Hongxue, YAO Dandan, FENG Zhiyong</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202008014&flag=1]]></guid><cfi:id>77</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Occurrence and Subgroup Identification of Cucumber mosaic virus in Luoyang Area]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202007014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was to identify the occurrence and subgroup types of Cucumber mosaic virus（CMV）on major vegetables in Luoyang City, and provide an important theoretical basis for the field diagnosis and prevention and monitoring to prevent the large-scale outbreak of CMV in this area.【Method】Samples with suspected virus symptoms collected from the Solanaceae, Cucurbitaceae and Leguminosae vegetables in Luoyang City, and were detected by RT-PCR with CMV primers, the obtained CMV CP gene sequences were analyzed by relevant biological software and the phylogenetic tree was constructed.【Result】Among 86 vegetable samples with suspected virus, CMV was detected in 13 samples from tomato, pepper, kidney bean and peanut, with a detection rate of 15.12%. The nucleotide homology of 4 CMV CP genes was 90.8%-98.8%, and it was found that the CMV isolates on these 4 crops belonged to CMV subgroup Ⅰ strains according to the phylogenetic tree construction of isolates in CMV subgroup Ⅰ and CMV subgroup Ⅱ .【Conclusion】CMV occurred in Solanaceae and Leguminosae vegetables in Luoyang area, and CMV Ⅰ may be the dominant virus, indicating that aphid prevention and control should be done in advance at the early stage of crop planting, and CMV Ⅰ disease resistance breeding should be actively carried out on relevant crops.]]></description>
<pubDate>2020/9/8 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[LI Yueyue, WANG Qianjin, TIAN Shan, WANG Jiali, ZHAO Xusheng]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>LI Yueyue, WANG Qianjin, TIAN Shan, WANG Jiali, ZHAO Xusheng</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202007014&flag=1]]></guid><cfi:id>76</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Changes of Endogenous Hormones Levels in Infected Plants Associated with Trema levigata Witches' Broom Disease]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202007015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The differences of six kinds of endogenous hormone contents between the diseased and asymptomatic branches and leaves of Trema levigata Hand.-Mazz. witches' broom disease induced by phytoplasma were determined, which laid a foundation for the research of pathogenesis of T. levigata Hand.-Mazz. witches' broom disease and provided theoretical basis for the prevention and treatment of this disease.【Methods】The infected samples with typical symptoms of witches' broom and healthy samples with normal morphology were collected from the same plant in the 
field of Xinping County, Yunnan Province. Liquid chromatography-tandem mass spectrometry（LC-MS/MS）was used to qualitatively and quantitatively detect the contents of 25 types of plant hormones in six categories（auxin, cytokinin, abscisic acid, jasmonic acid, salicylic acid and gibberellin）.【Results】A total of 24 hormones were detected, H2JA was not detected in both infected and healthy samples, cZ was not detected in infected samples, GA19 was only detected in infected samples, and GA20, GA24 and GA53 were only detected in healthy samples. The content of MESA was the highest, there were highly significant differences in contents of IAA, IBA, ICA, MEJA, JA, JA-ILE, MESA, SA and ABA, and significant 
differences in those of IP and tZ between infected samples and healthy samples. The contents of GA3, GA19, GA20, GA7, GA9 and GA15 in gibberellin changed greatly. The content of six kinds of plant hormones in infected samples decreased significantly and the C/A ratio increased.【Conclusion】The study showed that the contents of plant endogenous hormones in phytoplasma-infected tissues of T. levigata Hand.-Mazz witches' broom changed significantly. The main symptom of this phytoplasma disease was the witches' broom, which was often caused by the comprehensive influence of various pathogenic factors. Further studies were needed to determine which hormone imbalance was the main causative factor.]]></description>
<pubDate>2020/9/8 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[WAN Qionglian1, WANG Lianchun1, CHAO Kaixiang1, ZHAO Jing1, SU Fan2, HE Menglan2, CAI Hong]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>WAN Qionglian1, WANG Lianchun1, CHAO Kaixiang1, ZHAO Jing1, SU Fan2, HE Menglan2, CAI Hong</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202007015&flag=1]]></guid><cfi:id>75</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Isolation and Identification of Phomopsis vexans Harter and Optimization of Sporulation Conditions]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202006008&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Phomopsis blight of eggplant caused by Phomopsis vexans Harter is an important disease in the current eggplant industry. A sufficient amount of spores is the material basis for studying the spore germination of P. vexans and its pathogenic mechanism. This research is aimed to further simplify the current complex sporulation process and improve the sporulation efficiency.【Method】The tissue separation method was used to isolate and purify the pathogen. The morphological characteristics observation and rDNA-ITS sequence analysis were used to identify the pathogen. The liquid culture method was used to optimize the sporulation conditions of P. vexans.【Result】The rDNA-ITS sequence of P. vexans was obtained. The sequence had the closest genetic relationship with P. vexans through NCBI and sequence alignment, with a homology of 99%. After cultivation for one day under the same condition, the mycelial growth in the liquid medium was better. The spore production after culture at 28 ℃ for one day then 24 ℃ for three days was 1.2 × 1010 spores/mL, which was larger than the spore production（6 × 107 spores/mL）after culture at 24 ℃ for four days. The spore production at different culture temperatures were 2.5×107（20 ℃）, 1.2×1010（22 ℃）, 9.4×109（24 ℃）and 8.5×108（26 ℃）spores/mL, respectively. Under the same culture condition, the 25% glucose PDB medium had the highest spore production（1.2×1010 spores/mL）.【Conclusion】The pathogens of P. vexans were isolated and identified, and the sporulation conditions were optimized. The optimized sporulation conditions were as follows: in a 150 r/min shaker, using the 25% glucose PDB medium to culture at 28 ℃ for one day, and then culture at 24 ℃ for three days.]]></description>
<pubDate>2020/8/10 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[HENG Zhou1, SUN Xiaoqing1,2, HUANG Junlin1, SUN Baojuan1, LI Zhi-liang1, LI Ying1, LI Tao1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>HENG Zhou1, SUN Xiaoqing1,2, HUANG Junlin1, SUN Baojuan1, LI Zhi-liang1, LI Ying1, LI Tao1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202006008&flag=1]]></guid><cfi:id>74</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Research Progress of Plant Virus Disease Transmitted by Bemisia tabaci and Chemical Control]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202006009&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[Bemisia tabaci is a major worldwide pest that spreads plant virus diseases and seriously harms agricultural and forestry production. B. tabaci can transmit more than 400 plant viruses, mainly including Begomovirus of the family Geminiviridae, Crinivirus of the family Closteroviridae, Carlavirus of the family Betaflexiviridae and Ipomovirus of the family Potyviridae. Among them, Begomovirus is the main virus transmitted by B. tabaci, which accounts for about 90% of the transmitted viruses. Insect control is the main method to control such virus diseases. The main insecticides to control B. tabaci are bisamides and new nicotine. At present, although there is a lack of effective chemical agents for virus diseases, the research and development of antiviral agents has attracted wide attention. The types of virus diseases transmitted by B. tabaci and the chemical agents for pest and disease control were reviewed in order to provide ideas for the prevention and control of B. tabaci and its transmitted virus diseases.]]></description>
<pubDate>2020/8/10 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[GUAN Xue, HU Qiongbo]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>GUAN Xue, HU Qiongbo</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202006009&flag=1]]></guid><cfi:id>73</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Comparison of Physiological and Ecological Characteristics Between Borreria latifolia and Several Common Orchard Weeds]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202006010&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Borreria latifolia is suitable for green covering in the orchards of south China. The study is conducted to elucidate the physiological and ecological characteristics of B. latifolia and several common orchard weeds.【Method】The differences in seed morphology and germination characteristics, root characteristics, photosynthetic characteristics, and sensitivity to glyphosate between B. latifolia and three common weeds, Ageratum conyzoides, Bidens alba and Eleusine indica, were compared.【Result】The thousand seed weight of B. latifolia was 1.8-25.8 times than that of A. conyzoides, B. alba and E. indica. Meanwhile, seed germination rate of B. latifolia was greater than that of other weeds, and seedling growth of B. latifolia was also faster. The root-shoot ratio of B. latifolia was 70.1%-79.3% lower than that of other weeds. However, the diameter frequency（87.8%） of its root system in the range of 0-0.5 mm was 16.3%-21.6% higher than that of the other three weeds. Among the four species, B. latifolia had the lowest photosynthetic intensity, and its net photosynthetic rate（Pn） was 22.9%, 34.3%, and 64.3% lower than that of A. conyzoides, B. alba and E. indica, respectively, while its transpiration rate（Tr） and stomatal conductance（gs） were lower than those of A. conyzoides and B. alba, but higher than those of E. indica. B. latifolia was less sensitive to glyphosate than A. conyzoides and B. alba, and the 50% effective dose（ED50） of glyphosate on B. latifolia was 3.74 and 1.79 times of A. conyzoides and B. alba, respectively.【Conclusion】The characteristics of B. latifolia are fast growth ability at the seedling stage, shallow roots, low photosynthetic rates and glyphosate tolerance.]]></description>
<pubDate>2020/8/10 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[ZHANG Taijie, GUO Wenlei, ZHANG Chun, TIAN Xingshan]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>ZHANG Taijie, GUO Wenlei, ZHANG Chun, TIAN Xingshan</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202006010&flag=1]]></guid><cfi:id>72</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Establishment of Fluorescence LAMP Detection System for Pathogen of Moko Disease]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202005009&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The purpose of the study is to establish a real-time fluorescence qualitative detection method based on the LAMP technology and the specific conservative primers of the pathogen of Moko disease（Ralstonia solanacearum, race 2）. To solve the problems such as low specificity, low sensitivity and long time-consuming of qualitative detection of conventional PCR, an accurate and efficient qualitative detection within an hour can be achieved, which will provide technical support for the detection and quarantine of prevention and control of the disease.【Method】The sequence of gene target was determined and the Primer software was used to design two pairs of internal and external primers. The LAMP reaction system consisted of two pairs of primers, DNA polymerase with characteristics of strand displacement, template DNA, betaine, Mg 2SO4, reaction buffer, fluorescent dye etc. By using real-time fluorescence LAMP method, a lid-opening detection could be avoided after reaction and the sensitivity, specificity and repeatability of this method for the qualitative detection of sample DNA could be validated effectively.【Result】The designed primers could successfully amplify the target gene. The real-time fluorescence LAMP detection system had good sensitivity with specificity and strong repeatability for the pathogen of Moko disease, which was 10 times higher than that of ordinary PCR.【Conclusion】 The fluorescence LAMP detection system shows high specificity, high sensitivity, short time-consuming and low possibility of pollution for the detection of the pathogen of Moko disease.]]></description>
<pubDate>2020/7/15 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[YANG Leiliang1,2, GUAN Wei1, Sun Lixia2, Wu Yinger1, SHAN Zhenju1, WANG Zhanggen1, CHEN Dinghu1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>YANG Leiliang1,2, GUAN Wei1, Sun Lixia2, Wu Yinger1, SHAN Zhenju1, WANG Zhanggen1, CHEN Dinghu1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202005009&flag=1]]></guid><cfi:id>71</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Synergistic Toxicity and Field Control Efficacy of Tebuconazole and Fludioxonil against Neoscytalidium dimidiatum on Pitaya]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202005010&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was to clarify the synergistic toxicity and field control efficacy of tebuconazole and fludioxonil against Neoscytalidium dimidiatum on pitaya.【Method】The mycelial growth rate method was used to determine the in vitro toxicity efficacy of tebuconazole, fludioxonil and their compound fungicide on N. dimidiatum. The synergistic effect of the compound fungicide was evaluated by Wadley method. The field experiment of the compound was carried out to verify its control effect on pitaya brown rot disease.【Result】 Fludioxonil and tebuconazole showed good inhibitory effect against N. dimidiatum on pitaya, and the EC50 values were 0.0045 and 0.0671 mg/L, respectively. The compound of tebuconazole and fludioxonil exhibited an additive effect on N. dimidiatum. After 7 days of the last spraying with the active ingredient concentrations of 100 mg/kg and 500 mg/kg on pitaya plants for three times, the average control effects of tebuconazole and fludioxonil with the mass ratio of 8 ∶ 2 were 80.31% and 89.44%, respectively. And the average control effect of single tebuconazole was 80.24% and 89.71%, while the average control effect of single fludioxonil was 70.08% and 76.29%, respectively. After 15 days of the last spraying, the average control effect of tebuconazole and fludioxonil with the mass ratio of 8 ∶ 2 was 76.80% and 83.63%, respectively. And the average control effect of single tebuconazole was 74.63% and 81.22%, while that of single fludioxonil was 54.53% and 65.24%, respectively. The control effect of two active ingredient concentrations of tebuconazole and fludioxonil compound was higher than that of single fungicide.【Conclusion】The compound of tebuconazole and fludioxonil with the mass ratio of 8 ∶ 2 showed good inhibitory effect to N. dimidiatum on pitaya, and exhibited good control efficacy in the field, with non-toxicity to pitaya. Therefore, the compound fungicide with the mass ratio of 8 ∶ 2 could be promoted in pitaya production.]]></description>
<pubDate>2020/7/15 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[LAN Guobing1, 2, HE Zifu1, YU Lin1, TANG Yafei1, LI Zhenggang1, DENG Mingguang1, SHE Xiaoman1, 2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>LAN Guobing1, 2, HE Zifu1, YU Lin1, TANG Yafei1, LI Zhenggang1, DENG Mingguang1, SHE Xiaoman1, 2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202005010&flag=1]]></guid><cfi:id>70</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Establishment and Application of RT-LAMP Detection Method for Sugarcane Streak Mosaic Virus]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202004013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】In order to provide technical support for the detection of sugarcane streak mosaic virus（SCSMV）, a reverse transcription loop-mediated isothermal amplification（RT-LAMP）assay was established.【Method】A set of specific primers were designed from the conserved regions of coat protein gene sequences of SCSMV. The reaction conditions for RT-LAMP were optimized and the detection sensibility and specificity of RTLAMP were analyzed and compared. For fluorescence detection, the RT-LAMP reaction products were also visually detected by adding SYBR Green I dye to identify the results in a convenient and rapid way.【Result】A RT-LAMP assay was developed for the rapid detection of SCSMV, and the whole detection process could be completed in 60 minutes at 60-63℃ . The RT-LAMP established in this study had high sensitivity, which was 100 times higher than that of the traditional RT-PCR, and the lowest detection concentration was 167.9×10-5 ng. In addition, the method had high specificity and could be used for the specific detection of SCSMV. 【Conclusion】A sensitive, convenient and rapid RT-LAMP assay was established and used for field detection of SCSMV samples.]]></description>
<pubDate>2020/6/16 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[LI Zhanbiao, XIE Huiting, CUI Lixian, CAI Jianhe, QIN Bixia]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>LI Zhanbiao, XIE Huiting, CUI Lixian, CAI Jianhe, QIN Bixia</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202004013&flag=1]]></guid><cfi:id>69</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Analysis on CP Gene Sequence of Turnip Mosaic Virus Infecting Cabbage in Guizhou]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202004014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was to identify the occurrence and distribution of cabbage Turnip mosaic virus（TuMV）in Guizhou Province, and provide guidance for scientific prevention and control.【Method】Samples of cabbage virus disease were collected in Anshun City, Weining County and Xiuwen County of Guizhou Province, and TuMV was detected by enzyme-linked immunosorbent assay（ELISA）and RT-PCR. The consistency of TuMV CP gene sequences was analyzed by relevant biological software, and the phylogenetic tree was constructed.【Result】Among 152 samples of cabbage virus disease, 66 samples were tested positive, with a detection rate of 43.42%. The nucleotide homology of 15 TuMV CP genes was 88.70%-100%, and it was found that 14 isolates belonged to basal BR and 1 isolate belonged to world-B group according to the phylogenetic tree construction of isolates in 6 TuMV groups.【Conclusion】TuMV is common in different cabbage planting areas of Guizhou Province and basal-BR is the dominant toxic source for the infection of cabbage.]]></description>
<pubDate>2020/6/16 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[LIU Xuehui, LI Chun, CHEN Xiaojun, HE Haiyong, WANG Lishuang]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>LIU Xuehui, LI Chun, CHEN Xiaojun, HE Haiyong, WANG Lishuang</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202004014&flag=1]]></guid><cfi:id>68</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Changes of Endogenous Hormone Content in Resistant and Susceptible Near-isogenic lines of Rice after Infection with Xanthomonas oryzae pv.oryzicola]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202003012&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The objective was to study the changes of endogenous hormone content during the interaction 
between rice resistance, susceptible near-isogenic lines and pathogenic bacteria of Xanthomonas oryzae pv.oryzicola
（Xooc）to provide theoretical basis for the study of the resistance mechanism of bacterial leaf streak.【Method】Using 
rice resistant and susceptible near isogenic lines（LR19 and LS19）as materials, the treatment groups were inoculated by pricking with pathogenic bacteria of Xooc, while the control groups were inoculated with sterilized water. At 0, 24, 48 and 96 h 
after inoculation, the leaves of rice at the tillering stage were taken as samples to analyzed the changes in the content of salicylic 
acid（SA）, jasmonic acid（JA）, ethylene（ETH）, gibberellin（GA）and zeatin（ZT）.【Result】After inoculation, 
the average contents of SA, ETH and ZT in the treatment groups of LR19 were 8.32 μg/mL 220.06 nmol/L and 5.56 pmol/
L, respectively, while those in the control groups were 8.31 μg/mL 250.86 nmol/L and 6.4 pmol/L, respectively. The average 
contents of SA, ETH and ZT in the treatment groups of LS19 were 6.57 μg/mL, 128.35 nmol/L and 4.37 pmol/L, respectively, 
while those in the control groups were 6.27 μg/mL, 134.7 nmol/L and 4.96 pmol/L, respectively. For these three hormones, there 
were no significant differences between the treatment and control groups of both lines, but the contents in resistant material were 
always higher than those in susceptible material. After inoculation, the average contents of JA and GA in the treatment groups 
of LR19 were 2 316.0 pmol/L and 63.81 pmol/L, respectively, while those in the control groups were 1 174.33 pmol/L and 75.19 
pmol/L, respectively. The average contents of JA and GA in the treatment groups of LS19 were 3 043.08 pmol/L and 83.20 pmol/
L, respectively, while those in the control groups were 971.83 pmol/L and 66.27 pmol/L, respectively. For these two hormones, 
there were significant differences between the treatment and control groups of both lines, and the contents in resistant material 
were lower than those in susceptible material.【Conclusion】The contents of hormones SA, ETH and ZT in the resistant near isogenic lines were higher than those in the susceptible near-isogenic lines. Infection with Xooc induced the changes of JA and 
GA contents in both lines, which may be related to the BLS resistance.]]></description>
<pubDate>2020/6/1 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[何圣贤，万　瑶，覃雪梅，张　慧，刘　芳]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>何圣贤，万　瑶，覃雪梅，张　慧，刘　芳</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202003012&flag=1]]></guid><cfi:id>67</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Field Efficacy of Eight Insecticides on Spodoptera frugiperdain Guangdong and Their Evaluation]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202003013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The control effects and investment ratio of control efficacy of eight insecticides on Spodoptera 
frugiperda in Guangdong were compared.【Method】According to the Pesticide-Guidelines for the Field Effi cacy Trials, field 
trials were conducted in corn fields to evaluate the control effects of eight insecticides, such as lufenuron 50 g/L EC, emamectin 
benzoate 5% WDG and chlorantraniliprole 200 g/L SC, mamestra brassicae nuclear polyhedrosis virus 20 billion PIB/L SC, and 
etc.【Result】The highest control effects were 70.75%, 66.75%, 92.23%, 75.21%, 84.29%, 92.52%, 88.12% and 71.41% under 
treatments of deltamethrin 25 g/L EC, lambda-cyhalothrin 25 g/L EC, lufenuron 50 g/L EC, avermectin 1.8% EC, emamectin 
benzoate 5% WDG, chlorantraniliprole 200 g/L SC, mamestra brassicae nuclear polyhedrosis virus 20 billion PIB/L SC and 
beauveria bassiana 300 billion spore/g WP, respectively. The ratios of control effects and costs corresponding to the treatments 
above were 11.60, 5.56, 13.56, 8.05, 9.00, 18.76, 42.13 and 87.50, respectively.【Conclusion】Under the condition of limited pesticide budget, emamectin benzoate 5% WDG is recommended; under the condition of adequate pesticide budget, lufenuron 50 g/L 
EC and chlorantraniliprole 200 g/L SC are recommended. Mamestra brassicae nuclear polyhedrosis virus 20 billion PIB/L SC can be 
used for biological control of S. frugiperda larvae.]]></description>
<pubDate>2020/6/1 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[林少源，陈炳旭，徐　淑，全林发，池艳艳]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>林少源，陈炳旭，徐　淑，全林发，池艳艳</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202003013&flag=1]]></guid><cfi:id>66</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Study and Analysis on Resistance of Conventional Varieties to Rice Blast in Guangdong Province]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202002014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The types of rice varieties are diverse and complex. Scientific, reasonable, and accurate 
evaluation of the good rice varieties is of great significance for the selection of excellent varieties and their application and 
promotion. The data from five-years regional trials of conventional rice varieties in Guangdong Province was used to analyze the 
selection of varieties resistant to rice blasts and the use of resistance sources of rice blasts in Guangdong province.【Method】
The identification results of resistance to rice blast in early and late rice varieties that participated in regional trials in Guangdong 
Province from 2011 to 2115 were used, and the resistance of 149 rice varieties and parents of resistant varieties were analyzed to 
explore the resistance characteristics of tested varieties to rice blast in Guangdong.【Result】According to the data results, it 
was found that among 149 varieties, 84 varieties had resistance to rice blasts above resistance level, a proportion of 56.4%, and 
22 varieties were lower than susceptible level, accounting for about 14.8%. There were a total of 43 varieties above susceptible 
level as well as lower than resistance level, accounting for 28.9%. It shows that, in recent years, most of the varieties approved inGuangdong province have resistance to rice blast, and breeding units are paying more attention to rice blast resistance breeding and 
the use of germplasm resources resistant to rice blast.From the analysis on the resistance source of rice blast, 28 Zhan and Qingliu’ai 
1 are the two main rice blast resistant parents, which have derived up to 42 new rice varieties, accounting for 77.8% of the rice blast 
resistant varieties. They have become two major resistance sources for the breeding of conventional rice resistant to rice blast in 
Guangdong Province. 【Conclusion】According to the analysis on resistance sources of rice blast resistance cultivars, it shows that 
the selection of appropriate parents is very important for breeding new varieties resistant to rice blast.]]></description>
<pubDate>2020/4/26 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[钟春燕 1
，孟　醒 1
，王茂辉 1
，任　勇1
，邓沛飞 1
，陈志强 2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>钟春燕 1
，孟　醒 1
，王茂辉 1
，任　勇1
，邓沛飞 1
，陈志强 2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202002014&flag=1]]></guid><cfi:id>65</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Distribution and Influencing Factor of Ant Mound of Solenopsisinvicta under Three Habitats in Northern Guangdong]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202002015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The distribution characteristics of ant mound of Solenopsis invicta under different habitats in 
northern Guangdong were studied to provide scientific basis for the surveillance and control of S. invicta.【Method】The 
quadrat method was used to investigate the density and size of ant mound of S. invicta under three different habitats (i.e., 
wasteland, ridge and lawn), and the spatial distribution pattern of ant mound was analyzed by aggregation index method and 
Taylor power law. Moreover, the correlation analysis was conducted to explore the relationship between ant mound density 
and plant diversity on wasteland. 【Result】The ant mound density on the ridge was up to 18.67 mounds per 100 square 
meters (over ten times than that on lawn), which was significantly higher than that on wasteland and lawn. The perimeter of 
basal ant mound was similar in these three habitats, but the height of ant nest on wasteland was higher than that on ridge 
and lawn. Based on the results of aggregation indices and Taylor power law, the spatial distribution pattern of ant mound 
on wasteland and ridge basically belonged to uniform or random distribution, but the ant mound tended to be aggregateddistribution on lawn. Besides, the invasion of S. invicta in northern Guangdong has not caused a decline of plant diversity on 
wasteland.【Conclusion】Affected by factors such as human disturbance, the order of occurrence degree of S. invicta from 
severe to moderate under different habitats in northern Guangdong was ridge, wasteland and lawn. The spatial pattern of ant 
mound was basically aggregated distribution on lawn but tended to be uniform or random distribution on wasteland and ridge, 
such differences in distribution were mainly caused by human disturbance and nesting habits of S. invicta. The ant mound 
density of S. invicta was positively correlated with the plant biodiversity on wasteland, but the correlation was not significant.]]></description>
<pubDate>2020/4/26 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[郭　靖，刘锐英，孔令枝，肖　榕，黄红英]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>郭　靖，刘锐英，孔令枝，肖　榕，黄红英</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202002015&flag=1]]></guid><cfi:id>64</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[RNA Interference of Flightin Gene Mediated by BacteriallyExpressed dsRNA in Bactrocera dorsalis (Hendel)]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202002016&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was to explore the feasibility of feeding target gene dsRNA expressed by HT115-
mediated RNAi in Bactrocera dorsalis(Hendel) fl ightin gene.【Method】The RNAi fragment of fl ightin from B.dorsalis
was inserted into L4440 dsRNA interference vector, and transformed into E.coli HT115 (DE3). The dsRNA corresponding to 
fl ightin, designated as fl ightin-dsRNA, was expressed by IPTG induction.【Result】Real-time quantitative PCR analysis 
revealed that the expression of fl ightin in B.dorsalis was generally up-regulated in different degrees by feeding 10-fold 
concentrated bacterial solution expressing fl ightin-dsRNA to B.dorsalis. Among which, there were significant differences 
between females after feeding for 5, 10 and 20 days, males after feeding for 5 days and the control, and about 43% downregulation was induced in males after feeding for 15 days. The flight ability and chest muscle development of B.dorsalis
were not affected significantly. 【Conclusion】The feasibility by feeding E.coli HT115(DE3) expressing fl ightin-dsRNA to 
interfere with the fl ightin gene of B.dorsalis needs to be further confirmed.]]></description>
<pubDate>2020/4/26 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[袁瑞玲 1，2，郑传伟 2，3，冯　丹 1，2，王艺璇 2，杜春花 2，陈　鹏 2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>袁瑞玲 1，2，郑传伟 2，3，冯　丹 1，2，王艺璇 2，杜春花 2，陈　鹏 2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202002016&flag=1]]></guid><cfi:id>63</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Utilization Situation and Prospect of Gene xa5 AgainstPathotype V of Rice Bacterial Blight]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202001013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[In recent years, the strong virulence pathotypeⅤof rice bacterial blight grew up quickly in Southern 
China, which has become a major population and spread to the rice regions of Jiangsu and Zhejiang provinces in Southern 
China. Since pathotypeⅤcaused serious bacterial blight disease in rice production regions, it is urgent to breed and promote 
resistant varieties against pathotypeⅤ. The most economic and effective measure to control rice bacterial blight is to breed 
resistant cultivars for widely planting by using resistance genes. The Institute of Plant Protection, Guangdong Academy of 
Agricultural Sciences, and Agricultural Science Research Institute of Panyu District of Guangzhou used IRBB5 carrying 
the recessive xa5 gene from IRRI that resists pathotypeⅤto breed resistant varieties with rice blast resistance source, 
hybridization, multiple cross, pedigree selection and synchronous resistance evaluation. We successfully bred serials of new 
rice resistance varieties such as Baixiangzhan, Baigengzhan and Baisizhan, which showed resistance to bacterial blight strongvirulence pathotypeⅤ(scales 1-3), resistance to rice blast (mediate to high resistance), good grain quality (scale 3 of rice quality 
of Guangdong), and equivalent production with major cultivars (compared with region trial control cultivars of Guangdong). 
These new resistant varieties were promoted and planted in the strong virulence pathotypeⅤregion along the west coast of 
Guangdong, which showed favorable superiority and wide application prospect in controlling rice bacterial blight with resistance 
varieties.]]></description>
<pubDate>2020/4/1 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[成太辉 1
，陈　深 2，杨健源 2，朱小源 2，伍圣远 2，洪启金 1
，曾列先 2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>成太辉 1
，陈　深 2，杨健源 2，朱小源 2，伍圣远 2，洪启金 1
，曾列先 2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202001013&flag=1]]></guid><cfi:id>62</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Selection of Bactericides for ControllingBacterial Soft Rot of Banana]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202001014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was to screen bactericides for controlling soft rot of banana.【Method】The 
minimal inhibit concentration, detached leaves and pot experiments were adopted to study the control effects of different 
bactericides on soft rot of banana.【Result】Streptomycin sulfate, benzylpenicillin potassium and zhongshengmycin 
had strong inhibitory capacity, with minimal inhibitory concentrations of 0.2 μg/mL, 3.12 μg/mlL and 6.25 μg/mL, 
respectively. But copper oxychloride and thiodiazole copper had low toxicity to the bacteria, with the minimal inhibitory 
concentrations of 200 μg/mL and 400 μg/mL, respectively. According to the determination results of detached leaf 
method, the control effects of 400 μg/mL copper oxychloride, thiodiazole-copper and streptomycin sulfate were the best, 
with control rates of 97.67%, 96.34% and 95.12%, respectively. When copper oxychloride and thiodiazole copper were 
sprayed after being inoculated with pathogen in pot experiments, the control effects of 30% copper oxychloride SC 600 
times solution and 20% thiodiazole-copper SC 500 times solution were optimal, with control rates of 79.37% and 80.15% onthe 12th day after spraying. While the two bactericides were sprayed before being inoculated with pathogen in pot experiments, 
the control effects of 30% copper oxychloride SC 600 times solution and 20% thiodiazole copper SC 500 times solution were 
87.01% and 89.23% on the 12th day after spraying.【Conclusion】Copper oxychloride and thiodiazole copper could be 
recommended as green bactericides in controlling soft rot of banana.]]></description>
<pubDate>2020/4/1 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[沈会芳，蒲小明，杨祁云，张景欣，孙大元，林壁润]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>沈会芳，蒲小明，杨祁云，张景欣，孙大元，林壁润</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202001014&flag=1]]></guid><cfi:id>61</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Comparison of Utilization Rates and Droplets Distributionof Pesticides Sprayed by Electric and ManualSprayers in Rice Fields]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202001015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The effective utilization rates and droplets distribution effects of three pesticides sprayed on 
rice by manual sprayer and electric sprayer were compared.【Method】The plot experiments were carried out on pesticide 
utilization rate of late rice in two towns of Huiyang District and two towns of Boluo County of Guangdong Province. The tested 
pesticides included 50% pymetrozine water dispersible granule (WDG), 5% chlorpromazide suspension concentrate (SC) 
and 2% kasugamycin aqueous solutions (AS), and the allura red tracers were added into the pesticide diluents. Five point 
sampling method, droplet cards and spectrophotometer (501nm) were used in the research.【Results】The utilization rates 
of pymetrozine, chlorobenzamide and kasugamycin on rice sprayed by electric sprayers were 39.43%, 38.03% and 38.98%, 
respectively; while the rates by manual sprayers were 29.02%, 28.08% and 28.50%, respectively. Compared with those of the 
manual sprayer treatment, the effective deposition rates of three pesticides sprayed by the electric sprayer increased by 10.41, 
9.95 and 10.93 percentage points, respectively. Moreover, the effective deposition rates of the electric sprayer treatments were 
significantly higher than those of the manual spray treatments. The results of droplets deposition distribution assay indicated 
that, both of manual and electric sprayer treatments decreased gradually from top to bottom, and droplet deposition of different 
layers showed significant differences. The results of droplets diameter showed that, the droplets diameters of middle and lower 
layers on rice under the manual sprayer treatments were significantly higher than those of the electric sprayer treatments. The 
results of droplets quantity indicated that, the electric sprayer treatments showed a downward trend from bottom to top, and the 
droplet quantities of the electric sprayer treatments were also higher than those of the manual sprayer treatments.【Conclusion】
The effective utilization rates of three pesticides sprayed by electric sprayers were higher than 38% at the heading stage of late 
rice in Guangdong Province, which were 9.95-10.41 percentage points higher than those of manual sprayer treatments. And the 
atomization effect of electric sprayer was better than that of manual sprayer.]]></description>
<pubDate>2020/4/1 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[蒲小明 1
，陈锐明 2，周松丁 3，黄爱玲 4，路　征 5，张景欣 1
，沈会芳 1
，林壁润 1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>蒲小明 1
，陈锐明 2，周松丁 3，黄爱玲 4，路　征 5，张景欣 1
，沈会芳 1
，林壁润 1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202001015&flag=1]]></guid><cfi:id>60</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Research Progress in Barley Yellow Dwarf Virus and BYDV-GAV Strain in China]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202010013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[Barley yellow dwarf virus （BYDVs） belongs to Luteovirus, the Luteovirdae family. Wheat yellow dwarf disease caused by BYDV first occurred and was reported in the United States, and has been reported in other regions abroad. In China, the disease first occurred and was reported in Shannxi and Gansu in 1960. In recent years, it has also occurred in other regions of China. The disease is called a "cancer" on wheat crops, which has a great impact on wheat crop production. When confronted with severe damage, the outputs of wheat crops were reduced greatly. The classification of BYDV strains is based on the specificity of its transmission vector, that is, a virus strain can only be transmitted by one or two aphids. According to the classification system of the ICTV International Virus Commission, the virus strains identified internationally include BYDV-MAV, BYDV-PAV, BYDV-RMV, BYDV-SGV et al, and 4 strains have been identified in China, which are BYDV-PAV, BYDV-GPV, BYDV-GAV and BYDV-RMV. BYDV-GAV is a prevalent strain in China. This article mainly reviews the disease symptoms, strain differentiation, transmission vector, genome structure and function, disease prevention and control of BYDV, focusing on the research of resistance identification and resistance genes of BYDV-GAV. And then a certain theoretical basis is provided for the disease resistance breeding and control.]]></description>
<pubDate>2020/12/28 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[QIAO Shiying, YAN Jiahui, GUO Qingyun]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>QIAO Shiying, YAN Jiahui, GUO Qingyun</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202010013&flag=1]]></guid><cfi:id>59</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Main Pests and Diseases and Control Phenology Period of Two-cropping Grapes Under Facility Culture in Guangdong]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202010014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was conducted to comprehensively under the main insect pests and diseases and clarify control phenology of two-cropping grapes under facility culture in Guangdong.【Method】Interviews and routine surveys were carried out in 2016-2019. According to the principle of ‘as early and thoroughly as possible’, the key control phenology period should be determined according to the dynamics of the occurrence of main diseases and pest insects, corresponding grape phenology periods and prevention and control practices.【Result】Under the facility culture, powdery mildew was the most serious disease for two-cropping grapes in Guangdong, followed by canker disease, downy mildew, spikestalk brown spot, gray mold, sunscald, air burn, mycoplasmnoid. Thrips were the most serious pests, and the main species were Scirtothrips dorsalis Hood, Thrips tabaci Lindeman, Thrips palmi Karny, followed by Tetranychus urticae Koch, Spodoptera litura（Fabricius）, Adoretus sinicus Burmeister, and Colomerus vitis（Pagenstecher）. The main pests and diseases were quite different from those under open field cultivation. The control phenology focused on the dormancy period, the early flowering period, the young fruit period, the fruit expansion period, the fruit color change period, and the fruit maturity period.【Conclusion】The main insect pests and diseases and control phenology of two-cropping grapes under facility culture were different from those under open cultivation. It had the characteristics of overlapping occurrence, longer damage period, two or more peak periods of multiple insect pests and diseases, and the same kind of insect pests and diseases were more likely to break out. In regard to two-cropping grapes under facility culture, the main insect pests and diseases should be controlled during the critical period, focusing on clearing the orchard during the dormant period, eliminating the source of disease and insects, and carrying out targeted pest control in the key periods such as the flowering and fruiting periods.]]></description>
<pubDate>2020/12/28 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[TAN Delong，NIE Jun，ZHANG Changyuan，SHI Liangliang，LI Yanhong，ZHAO Junhong，ZHENG Jinrong，XIE Yuming]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>TAN Delong，NIE Jun，ZHANG Changyuan，SHI Liangliang，LI Yanhong，ZHAO Junhong，ZHENG Jinrong，XIE Yuming</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202010014&flag=1]]></guid><cfi:id>58</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Comparison of Trapping Effects and Population Density of Fruit Fly（Bactrocera dorsalis）in Pomelo Orchards with Different-habitats During Fruit Ripping]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202010015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was conducted to understand the population density and activity rule of Bactrocera dorsalis in pomelo orchards with different habitats in fruit ripening period, and to explore the trapping effects of different sticky cards in different directions and under different temperature environments on B. dorsalis.【Method】The monitoring and trapping experiments were carried out in two pomelo orchards with different habitats from September to November, 2019.【Result】One way ANOVA showed that the number of B. dorsalis trapped by every bright yellow sticky card in natural pomelo orchard was 24.84±1.90, and that in standard orchard was 4.29±0.44. The trapping effect was significantly higher 
than that of yellow and blue cards. The number of B. dorsalis trapped in natural pomelo orchard was significantly higher than that in standard orchard. The average number of B. dorsalis trapped in the south of the natural orchard was 12.01±2.02, and that of the standard garden was 2.52±0.44, both of which were higher than those in other directions. There was a negative correlation between temperature and population density of Bactrocera dorsalis in standard orchard. The number of B. dorsalis trapped in November was significantly higher than that in September and October.【Conclusion】The bright yellow sticky card had obvious control effect on fruit fly in pomelo orchard. The prevention work of standard orchard should be done in advance according to the population change rule of B. dorsalis under natural conditions in fruit ripening period, and comprehensive management should be carried out according to the local conditions in pomelo orchards.]]></description>
<pubDate>2020/12/28 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[LAN Muxiang, YANG Hesheng, XU Liangzheng, LIU Huina, ZENG Xianlu]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>LAN Muxiang, YANG Hesheng, XU Liangzheng, LIU Huina, ZENG Xianlu</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202010015&flag=1]]></guid><cfi:id>57</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Development and Application of 21% Fluoroglycofen-glufosinate ME]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202108012&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】 The high effective and broad-spectrum herbicide, 21% Fluoroglycofen-glufosinate ME, was 
developed and applied in the control of non-arable weeds, and its physical and chemical properties were determined.【Method】
The optimal combination method was used to screen out different surfactants to determine the optimal formula. Control 
effect of 21% Fluoroglycofen-glufosinate ME against non-arable weeds was studied by field tests. 【Result】The optimal 
formula of 21% Fluoroglycofen-glufosinate ME（mass fraction）was: 20% Glufosinate-ammonium, 1% Fluoroglycofenethyl, 7% cyclohexanone, 10% n-butanol, 20% emulsifier（OA-TAPO∶alkyl glucoside∶betaine = 3∶2∶1）, 
and water to made up for 100%. Field test results showed that, 21% Fluoroglycofen-glufosinate ME with dosages of 270-
540 mL/ hm2
 had obvious control effect on non-arable weeds. The coverage control effects against gramineous weeds were 
80.41%-83.47%（treatment after 5 d）, 92.69%-94.55%（treatment after 10 d）, 93.64%-96.04%（treatment after 30 
d）and those against broadleaf weeds were 84.44%-88.13%（treatment after 5 d）, 95.61%-96.72%（treatment after 10 
d）, 97.59%-98.45%（treatment after 30 d）, respectively. The relative fresh weight control effect could reach 93.65%-
96.09% and 97.37%-98.51%, respectively.【Conclusion】21% Fluoroglycofen-glufosinate ME can broaden the herbicide spectrum and effectively control non-arable weeds. It shows better performance on quick-acting property and continuous effects 
than those of 20% glufosinate-ammonium SL and 10% fluoroglycofen-ethyl EC.]]></description>
<pubDate>2021/9/24 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[彭 静，陈维洪，黄雪萍，蒋殿君，梁丽丽，邓晓珊]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>彭 静，陈维洪，黄雪萍，蒋殿君，梁丽丽，邓晓珊</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202108012&flag=1]]></guid><cfi:id>56</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Identification and Selection of Fresh Corn Germplasm Resources with Resistance to Southern Corn Rust]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202107014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The resistance of fresh corn germplasm resources to southern corn rust was explored with 
a view to provide materials and theoretical references for resistant varieties.【Method】Investigation was conducted to 
analyze the resistance of different inbred lines to southern corn rust with the method of visual observation under field natural 
conditions and artificial inoculation.【Result】There were significant differences between different inbred lines of fresh 
corn. Under field natural conditions, only 1.11% of 710 tested inbred lines showed highly resistant level, and 8.34% of which 
showed resistant level. About 77.75% of inbred lines showed middle resistant and susceptible levels, while the rest showed 
highly susceptible level. After artificial inoculation, 5 inbred lines of fresh corn that highly resistant to the southern corn rust 
were screened out, including three of waxy corn, one of sweet corn and one of waxy-sweet corn. Preliminary genetic analysis 
of the resistant inbred line 09N1-1-1 showed that the separation ratios of F2
 and BC1F1
 populations were 3 ∶ 1（χ2
=2.473，
P=0.116）and 1 ∶ 1（χ2
=0.084，P=0.772）respectively, revealing the resistance of inbred line 09N1-1-1 was probably 
controlled by a single dominant gene.【Conclusion】The study demonstrated that the current fresh corn resources have 
high resistance to southern corn rust. Therefore, the selection and utilization of disease resistant germplasm has potential 
application in variety breeding currently.]]></description>
<pubDate>2021/8/26 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[田耀加，王秋燕，吴 蓓，叶伟忠，陈红弟]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>田耀加，王秋燕，吴 蓓，叶伟忠，陈红弟</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202107014&flag=1]]></guid><cfi:id>55</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Technical Mode of Reduction and Synergism for Citrus Chemical Fertilizers and Pesticides Application in Guangdong]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202107015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[Guangdong is an important citrus production area in China, and the citrus industry is seriously affected by 
the disease Huanglongbing. In the citrus production, fruit growers are highly dependent on the input of chemical fertilizers 
and pesticides. In recent years, with the improvement of citrus varieties and the optimization and adjustment of industrial 
structure, the cultivation technology level of citrus has been increasing year by year, but there are still some problems, such 
as excessive fertilization, unreasonable fertilization, extensive management, pesticide abuse, low utilization rate of fertilizers 
and pesticides, which have brought about ecological environmental pollution and citrus fruit quality and safety. Currently, 
the application amounts of chemical fertilizer and pesticide in citrus production areas in Guangdong are high, and the ways 
of application are unreasonable, which have great potentials for reduction and improvement. In order to improve the current 
situation of fertilizers and pesticides application in citrus production areas in Guangdong province, according to the idea of “scientific application amount, substitution reduction, synergistic increase”of scientific application amount in citrus orchard, 
through the selection, integration and demonstration of key technologies in the field for many years, and the optimization and 
integration of related supporting measures, a technical mode of reduction and synergism for citrus fertilizers and pesticides 
application in Guangdong province was established. The technical model formulated the key technical points of the integration 
of water and fertilizer, the substitution of organic fertilizer for chemical fertilizer, accurate detection and prediction of diseases 
and insects, the coordinated control of multiple diseases and insects in the same suitable period, the rational application 
of synergistic agents, and the weeding by ground cover, which has been applied and promoted in citrus production areas in 
Guangdong, effectively reducing the dosages of chemical fertilizers and pesticides, and achieving good results in the prevention 
and control of main diseases and insects, yield and quality. It provides scientific technical supports for the healthy and 
sustainable production of citrus in Guangdong.]]></description>
<pubDate>2021/8/26 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[赖　多，邵雪花，肖维强，刘传和，贺　涵，匡石滋]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>赖　多，邵雪花，肖维强，刘传和，贺　涵，匡石滋</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202107015&flag=1]]></guid><cfi:id>54</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Pathogen Isolation and Identification of Nigrospora Blight 
Disease on Sugarcane and Screening of Fungicides]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202106011&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was conducted to identify the pathogen causing Nigrospora blight on sugarcane, 
analyze the pathogen biological characteristics, design detection primers for the pathogen Nigrospora sphaerica, screen out 
fungicides which can effectively inhibit the pathogen N. sphaerica, and to provide guidance on field control of Nigrospora
blight on sugarcane.【Method】The pathogen of Nigrospora blight on diseased sugarcane leaves collected from Panyu 
District, Guangzhou City, Guangdong Province was purified and cultured by tissue separation method. After 5 days of 
culture, the morphology of mycelium and spores was observed by using an optical microscope. The fungi were determined 
for pathogenicity, and the pathogenic strains were amplified and sequenced by PCR with the primers ITS1/ITS4; then, 
the software MRGA 7.0 was used to construct a phylogenetic tree based on ITS sequence with the maximum likelihood method and sequence-specific detection primers Ng1F/Ng1R and Ng2F/Ng2R were designed according to the obtained ITS 
sequence. The indoor control effects of 7 different fungicides on N.sphaerica were screened by the mycelium growth rate 
method. The antibacterial effect of N.sphaerica, which is the pathogen of Nigrospora leaf blight on sugarcane, was screened. 
【Result】Through the study on morphological and molecular identification as well as biological characteristics, it was 
determined that the pathogen causing Nigrospora blight on sugarcane was N.sphaerica, which had the fastest colony growth 
rate at the temperature of 25 ℃ and pH of 7. The PCR method confirmed that the primers Ng1F/Ng1R could be used to detect 
the pathogen of sugarcane Nigrosporium blight. The results of the indoor antibacterial test showed that 0.16 mg/L of Prochloraz 
and 2.4 mg/L of Sporgon had the best antibacterial effect on N. sphaerica, and the antibacterial effect could reach over 93%.
【Conclusion】The pathogen causing sugarcane seed-borne disease Nigrospora blight is N.sphaerica. Ng1F/Ng1R can be 
used to detect the pathogen of N.sphaerica. Prochloraz and Sporgon agents can be used as the optimal fungicides for N.sphaerica
in the field or seedling detoxification treatment agent.]]></description>
<pubDate>2021/7/21 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[崔一平，彭埃天，凌金锋，宋晓兵，程保平，陈　霞]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>崔一平，彭埃天，凌金锋，宋晓兵，程保平，陈　霞</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202106011&flag=1]]></guid><cfi:id>53</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Screening of Plant Immune Inducer Against Tobacco Bacterial 
Wilt and Determination of Its Control Effect]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202106012&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】In order to explore the plant immune inducers which can be used to control tobacco bacterial 
wilt, the antibacterial activity and control effects of four kinds of inducers were screened and determined.【Method】
The control effects were determined and analyzed by using plate inhibition method and potted plant experiments, and the 
control effects of different plant immune inducers against tobacco bacterial wilt were studied through the determination of 
minimum inhibition concentration（MIC）and minimum bactericidal concentration（MBC）value.【Result】Aminooligosaccharin and NANO ZnO showed a certain inhibitory effect on Ralstonia solanacearum in the plate tests. The 5 000 
µg/mL amino-oligosaccharin had the best antibacterial effect with the inhibition zone diameter of 35.2 mm, and its MIC and 
MBC were 1 000 µg/mL and 2 000 µg/mL, respectively. Different concentrations of Zhinengcong and Atarin did not show any 
inhibitory effect. However, the potted plant experiments showed that Zhinengcong had the best prevention and control effect 
on bacterial wilt. The disease entered the peak period on the 10th day after inoculation, and the control effect reached 57%, which was significantly higher than the 37% control effect of streptomycin treatment in the same period. Moreover, Zhinengcong 
treatment could delay the occurrence of the disease. The control effect of amino-oligosaccharin with significant antibacterial 
effect on the plate was only 13%, and the control effects of NANO ZnO and Atarin were 3% and 15%, respectively, with poor 
control effects.【Conclusion】Plant immune inducer Zhinengcong had a great potential for biocontrol and can be used for green 
prevention and control of tobacco bacterial wilt.]]></description>
<pubDate>2021/7/21 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[薛　梅，曾婉琳，张　荣，冯淑杰]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>薛　梅，曾婉琳，张　荣，冯淑杰</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202106012&flag=1]]></guid><cfi:id>52</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Preliminary Study on Biocontrol Effect of the Bacterial Suspension 
of PP19 and SI17 Against Litchi Downy Blight]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202106013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【O bjective】New and efficient biocontrol suspensions that could prevent fruit postharvest diseases such 
as litchi downy blight were screened out to reduce the loss of litchi during transportation and delay browning, and reveal the relevant physiological mechanisms of biocontrol efficacy.【Method】The bacterial suspensions of Bacillus amyloliquefaciens 
PP19 and Exiguobacterium acetylicum SI17 were sprayed onto the litchi fruit for pre-treatment at the postharvest in the 
laboratory. Then, a suspension of Peronophythora litchii（SC18）24 hours after pre-treatment. Subsequently, the disease 
severity of litchi fruit was observed and the enzyme activity or substance contents corresponding to peel at different time 
points were detected.【Result】The suspensions of PP19 and SI17 significantly reduced the litchi downy blight, and the 
control efficacy of PP19 was 39.59%-88.76% while that of SI17 was 18.08%-91.01%. Their bacterial suspensions increased 
the activities of CAT, SOD, GLU, PAL and PPO in peel and the content of anthocyanin while decreased the activity of 
anthocyanidase and the content of H2O2
 and total phenol in peel. SI17 increased much more chitinase activity compared 
with that of PP19.【Conclusion】According to the results mentioned above, it is speculated that the effect of two biocontrol 
suspensions on disease prevention may be related to the change of related enzyme activities or substance contents to disease 
resistance in peel.]]></description>
<pubDate>2021/7/21 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[郑　丽，张海鹏，喻国辉，黄石连]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>郑　丽，张海鹏，喻国辉，黄石连</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202106013&flag=1]]></guid><cfi:id>51</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Reproductive System and Oogenesis of Propylea japonica]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202106014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】 Propylea japonica is an important natural enemy of agriculture and forestry, which can prey 
on aphid, whitefly, psyllid and other pests. The study of the reproductive system and oogenesis of P. japonica will provide 
scientific data support for its artificial breeding.【Method】The reproductive system and oogenesis of the P. japonica were 
observed with optical microscope by paraffin section technique and anatomical method. 【Result】The male reproductive 
system include 1 pair of testis, 1 pair of paragonia glands, 1 pair of vas deferens, seminal vesicle and ejaculatory duct. The 
female reproductive system include 1 pair of ovaries, 1 pair of lateral oviducts, median oviduct, spermatheca and gential 
chamber. Each side of ovary contains 11-13 ovarioles. According to the yolk precipitation and the morphology change of 
follicle cells, oogenesis can be divided into prime, middle, transitional and final stage.【Conclusion】The structure of ovary, 
the numbers of ovarioles and oocytes help to explain the oviposition quantity of P. japonica. Unsuitable rearing conditions may inhibit the development of ovary and lead to the change of oviposition quantity. Therefore, in order to realize the large-scale 
application of P. japonica, the optimal nutritional and environmental conditions should be created to ensure the production of as 
many mature eggs as possible.]]></description>
<pubDate>2021/7/21 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[刘志伟，钟国辉，王永跃，王兴民，毕可可]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>刘志伟，钟国辉，王永跃，王兴民，毕可可</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202106014&flag=1]]></guid><cfi:id>50</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Study on Biological Characteristics of Colletotrichum acutatum in Pepper]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202105014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Pepper anthracnose caused by Colletotrichum acutatum is an important factor restricting the pepper industry in Nanxiong, Guangdong Province. The biological characteristics of C. acutatum were studied to provide references for the control of pepper anthracnose.【Method】The pathogenicity of C. acutatum to different kinds of peppers were determined by artificial inoculation. The effects of temperature, pH, light, medium, carbon source, nitrogen source on C. acutatum were measured by mycelium growth method and microscopic examination.【Result】C. acutatum was pathogenic to red cluster pepper, line pepper, bell pepper, spiral pepper, beauty pepper, yellow skin pepper, green skin pepper, wrinkled skin pepper and pickled pepper. Among nine kinds of peppers, line pepper was more sensitive to C.acutatum and it could be infected without wounds. The suitable temperature for mycelium growth of C. acutatum was 24-28 ℃ . Mycelium growth was the fastest at 26 ℃ , and spore quantity was the largest at 30 ℃. The suitable pH for mycelium growth and spore production was 5.0 - 9.0. Different light conditions had little effect on mycelium growth, but light promoted spore production. The optimum medium for mycelium growth was PDA. Pepper fruit juice medium promoted spore production. Soluble starch, glucose and mannitolare were beneficial to mycelium growth and spore production, while sucrose was good for mycelium growth but bad for spore production. Organic nitrogen source beef extract was optimum to mycelium growth and spore production. 【Conclusion】C. acutatum was highly pathogenic to different kinds of peppers, and it could infected line pepper without wounds. The biological characteristics of C. acutatum were different from those of C.gloeosporioides and C.capsici.]]></description>
<pubDate>2021/7/4 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[沈会芳，杨祁云，谢祥恩，邓海滨，陈凤凤，孙大元，蒋尚伯，林壁润]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>沈会芳，杨祁云，谢祥恩，邓海滨，陈凤凤，孙大元，蒋尚伯，林壁润</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202105014&flag=1]]></guid><cfi:id>49</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Field Control of Bactrocera dorsalis by Artificial Release with Diachasmimorpha longicaudata（Ashmead）]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202105015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The field control of Bactrocera dorsalis by artificial propagation with Diachasmimorpha longicaudata was studied.【Method】In the experiment, a release zone and a control zone were set up, and three releasing points were set in the release zone. The artificially propagated D. longicaudata was released according to the ratio of 1∶10 of the 2nd and 3rd instar larvae of the female bee and B. dorsalis. Through biological observation experiment, the field parasitic rate and colonization of the artificially propagated D. longicaudata to B. dorsalis were studied.【Result】Artificial releasing of D. longicaudata could rapidly increase its parasitism rate in the field, reaching 5.97% on average, with the ratio of male to female close to 1. However, with the passage of time after releasing, the parasitic rate and the ratio of male to female decreased gradually, and the emergence rate of B. dorsalis increased gradually.【Conclusion】The D. longicaudata（Ashmead）is an important parasitic wasp for B. dorsalis larvae, and increasing its population in the field by artificial means is one of the important means in biological control at present. However, artificially propagated parasitic wasps are usually a large number of indoor population. Due to the complex field environment, their adaptability, parasitism rate and sex ratio in the field need further research.]]></description>
<pubDate>2021/7/4 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[刘吉敏，黄其椿，邓铁军，杨志强，陆 温]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>刘吉敏，黄其椿，邓铁军，杨志强，陆 温</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202105015&flag=1]]></guid><cfi:id>48</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Advance in Insects Reproductive Fitness]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202104012&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[Insect has become the most diverse and widely distributed group in the animal kingdom. Reproduction 
is an important way for insects to ensure the continuation and prosperity of the population. Due to its strong reproductive 
ability and special reproductive system, female insects can store sperm obtained from males during mating through the 
organ of spermatheca. The females can delay the fertilization of eggs and control their fertilization time so as to avoid time 
and environment that are not conducive to the survival of their offspring. After finding a suitable environment and host, 
the flow of semen will be controlled to fertilize the eggs to ensure the hatching rate of the eggs and the survival rate of the 
larvae. Reproductive fitness is an important index to measure insect adaptation to nature. In nature, insects usually adjust 
and adapt to the changes of external adverse environmental factors and their own factors by changing the way of reproductive 
fitness. Based on the results of a large number of studies on insect reproduction behavior by domestic and foreign scholars in 
recent years, this article reviews the reproductive fitness of insects from two aspects: environmental factors and insects' own 
factors. This paper discusses the influences of external environmental factors in aspects of temperature, humidity, light, food 
and density, and the change of insects' own factors in aspects of body size, multiple mating, mating experience and delayed 
mating on its reproductive fitness and the application of the corresponding research results in practical production. In 
addition, it also points out the problems of the current researches in order to provide directions for related further researches 
and provide ideas for finding new pest control measures in the field.]]></description>
<pubDate>2021/5/27 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[易小龙，王小云，郑霞林，陆　温]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>易小龙，王小云，郑霞林，陆　温</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202104012&flag=1]]></guid><cfi:id>47</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Evaluation on Field Control Effect of Chlorantraniliprole Against Sugarcane Termite]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202104013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was carried out to understand the field control effect of chlorantraniliprole 
against sugarcane termite.【Method】The field control effects of two different doses of chlorantraniliprole applied by 
mixed fertilizer broadcasting method on sugarcane termite were studied in 2018-2019. The plants damaged by sugarcane termite were investigated before harvest (in November), the damaged plants and effective sugarcane stems were investigated 
and the sugarcane yield was measured when harvested. The control effects on sugarcane termite and the economic benefits 
were analyzed.【Result】There was no significant difference in the damaged plant rates of sugarcane termites treated by 
chlorantraniliprole 5% with 7.5 kg/hm2
 and chlorantraniliprole 4% with 7.5 kg/hm2
, and both significantly lower than that 
of sugarcane termite treated by imidacloprid 15% with 7.5 kg/hm2
 at Banmai Village test site, while there was no significant 
difference in the damaged plant rates of sugarcane termites treated with the three pesticides at Qu'ao Village test site. On the 
whole, the control effect of chlorantraniliprole 5% with 7.5 kg/hm2
 on sugarcane termite was better. The damaged plant rate of 
termite treated by chlorantraniliprole 5% with 7.5 kg/hm2
 was under 3%, with the field efficacy of over 80%. Compared with the 
control, the sugarcane yield under treatment of chlorantraniliprole 5% with 7.5 kg/hm2
 increased to 19.2-31.5 t/hm2
, and the net 
income increased to 8 784-15 180 yuan/hm2
, with obvious increases of yield and income. 【Conclusion】Chlorantraniliprole 5%
(Guizhong no.2) with 7.5 kg/hm2
 has better control effect on sugarcane termite with significant benefits, which can be used as the 
pesticide for controlling sugarcane termite in cane areas where this pest seriously damage.]]></description>
<pubDate>2021/5/27 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[李德伟，江凤兰，农振益，梁　阗，罗亚伟，苏云武，谭宏伟，覃振强]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>李德伟，江凤兰，农振益，梁　阗，罗亚伟，苏云武，谭宏伟，覃振强</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202104013&flag=1]]></guid><cfi:id>46</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Study on Dry Heat Treatment of Colletotrichum on Chili Pepper]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202104014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The effects of dry heat treatment on dominant species of Colletotrichum - C. truncatum and C. 
acutatum on chili pepper were explored.【Method】By setting different temperatures and time, the growth status of mycelia 
and spores of C. truncatum and C. acutatum in vitro and the germination potential and germination rate of chili pepper 
seeds under different dry heat treatments were determined and analyzed.【Result】The inhibition rate of mycelia and spores 
growth of the two Colletotrichum species could reach 100% when they were dried at 55 ℃ for 1 h. Meanwhile, chili pepper 
seeds under treatment at 55-66 ℃ for 1-24 h could effectively increase seedling emergence rate.【Conclusion】The growth 
of Colletotrichum could be effectively inhibited and the germination rate of chili pepper seeds could be improved under this 
dry heat treatment condition (at a combined interval of 55-66 ℃ and 1-24 h). The dry heat treatment conditions screened by 
the current study can provide reference basis for the control of vegetable seeds borne fungi, especially Colletotrichum.]]></description>
<pubDate>2021/5/27 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[程唤奇，骆清兰，郑小玲，杨玲丽，孙　丹，刁永朝，胡茂林]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>程唤奇，骆清兰，郑小玲，杨玲丽，孙　丹，刁永朝，胡茂林</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202104014&flag=1]]></guid><cfi:id>45</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Control Effect of 5% Emamectin Benzoate Water Dispersible Granule Against Lepidoptera Pests in Field]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202104015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was carried out to evaluate the control effect of 5% emamectin benzoate water 
dispersible granule (WG) against Lepidoptera pests in field.【Method】Field plot test and five point sampling method 
were adopted to investigate and calculate the field control of 5% emamectin benzoate WG against Spodoptera exigua and 
Cnaphalocrocis medinalis.【Result】After spraying for 7 d, the control effects of 5% emamectin benzoate WG (active 
ingredient, ai) of 7.5 g/hm2
, 12.0 g/hm2
 and 15.0 g/hm2
 against S. exigua were 82.87%, 89.37% and 91.02%, respectively. 
After spraying for 14 d, the control effects of 5% emamectin benzoate WG (ai) of 7.5 g/hm2
, 12.0 g/hm2
 and 15.0 g/hm2
 against 
C. medinalis were 90.32%, 94.20% and 98.03%, respectively. Significant difference analysis results indicated that the 7 d 
and 14 d control effects of the 5% emamectin benzoate WG of 12.0 g/hm2
 treatment against S. exigua were higher than those of 5.7% emamectin benzoate emulsifiable concentrate (EC) treatment. The 14 d control effect of the 5% emamectin benzoate WG 
of 12.0 g/hm2
 treatment against C. medinalis was higher than that of 5.7% emamectin benzoate emulsifiable concentrate (EC) .
【Conclusion】The 5% emamectin benzoate WG showed good field control against S. exigua and C. medinalis. Moreover, it 
had a long duration, no harm to crops and no adverse effects on beneficial organisms.]]></description>
<pubDate>2021/5/27 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[蒲小明，王爱臣，林壁润，莫俊锐，邓海滨，张景欣，沈会芳，杨祁云]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>蒲小明，王爱臣，林壁润，莫俊锐，邓海滨，张景欣，沈会芳，杨祁云</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202104015&flag=1]]></guid><cfi:id>44</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Investigation and Detection of Main Diseases on Gonggan in Renhua County]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202102012&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Gonggan（Citrus reticulata blanco var. Gonggan）production is one of the pillar 
industries in Renhua County, Shaoguan City, Guangdong Province. In recent years, the planting area of Gonnggan has been 
scaled up gradually. In 2019, Renhua County Citrus Industrial Park was selected as the one of the second batch provincial 
modern agricultural industrial parks in Guangdong Province. However, the investigations and researches of major diseases 
on Gonggan in the region have not been reported in details.【Method】The QPCR（probe）detecting system and（RT—
PCR）method were used for the detection of Huanglongbing（HLB）and citrus virus diseases（Citrus tristeza virus, CTV; Citrus tatter leaf virus, CTLV; Citrus exocortis viroid, CEVd; Citrus yellow vein clearing virus, CYVCV）separately on 150 
samples from Renhua County. Besides, some of the fungal diseases in the field were more severe, therefore, conventional fungal 
separation methods and the common primer transcription interval of fungi, ITS, were used to isolate the main pathogens and 
identify the molecular biology of diseased samples collected from the region. 【Result】The average positive rate of HLB on C. 
reticulata blanco var. Gonggan samples collected in April 2019 was 13.2% and below 17% in October 2019 except for those 
in the unsupervised garden on Huangkeng Village, Huangkeng Town. It is worth noting that the positive detection rate of citrus 
HLB rose from 18.2% in April 2019 to 87.5% in October, while that in the orchards of Gebu Village, Danxia Street increased 
from 6.7% to 14.3%. Meanwhile, the average positive detection rates of CTV in April 2019 and October 2019 were 41.2% and 
100%, respectively. None of other virus diseases were detected. Besides, citrus cancer, citrus anthracnose and citrus brown 
spot disease also occurred in this region, and they were more serious in some individual orchards.【Conclusion】There is a 
certain degree of occurrence of citrus HLB in Renhua County. The damages of HLB on citrus industry can be reduced and the 
abundant year and production life of citrus orchards be extended as much as possible through comprehensive prevention and 
control measures, such as the classified control of high-quality and high-yield sustainable development with strengthening 
field management as the focus. However, CTV on C. reticulata blanco var. Gonggan has a high incidence rate, which suggests 
that the detection of CTV shall be conducted during citrus seedling production or before planting. And it is recommended to 
strengthen the scientific prevent and control of citrus anthracnose and citrus brown spot disease, rational drug use and reduction 
in chemical pesticides in this region.]]></description>
<pubDate>2021/3/8 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[陈　霞，崔一平，彭埃天，宋晓兵，程保平，凌金锋]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>陈　霞，崔一平，彭埃天，宋晓兵，程保平，凌金锋</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202102012&flag=1]]></guid><cfi:id>43</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Pathogen Identification of Anthracnose Disease on Coffeaarabica in Guangdong Province and Screening of Fungicides]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202102013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was conducted to clarify the pathogen causing anthracnose disease of Coffea 
arabica in Guagdong Province, and to screen out control fungicides.【Method】Tissue isolation method was used to isolate 
and purify the pathogen, and pathogenicity test was conducted by using Koch’s postulates. The pathogen was identified 
based on morphological characteristics and analysis of phylogenetic sequences（ITS, TUB2, GAPDH, CHS, ACT and GS）. 
Moreover, the inhibitory effects of four common fungicides on the pathogen were measured according to mycelial growth.
【Result】The obtained strains were identified as Colletotrichum fructicola, C. siamense and C. musicola. The results of 
pathogenicity assay indicated that all the selected isolates could infect leaves. In vitro test on two highly pathogenic strains 
（C. fructicola CA-13 and C. siamense CA-16）showed that prochloraz, pyraclostrobin and thiophanate-methyl strongly 
inhibited mycelium growth and their EC50 values were under 0.1 mg/L.【Conclusion】The dominant pathogen causing 
anthracnose disease on C. arabica leaves were C. fructicola and C. siamense; prochloraz, pyraclostrobin and thiophanatemethyl could be used to control this disease as the first choice.]]></description>
<pubDate>2021/3/8 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[徐丹丹 1,2，石力允 2，张　羽 1
，林泽勉 2，姜子德 2，乔　方 1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>徐丹丹 1,2，石力允 2，张　羽 1
，林泽勉 2，姜子德 2，乔　方 1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202102013&flag=1]]></guid><cfi:id>42</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Spatial Distribution of Spodoptera frugiperaLarvae in Corn Fields of Hainan Province]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202102014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】 The study was conducted to analyze the spatial distribution of Spodoptera frugiperda（J. 
E. Smith） larvae in corn fields of Hainan Province.【Method】Six aggregation indexes, Taylor power law and Iwao m*-m
regression method were used to analyze the spatial distribution of S. frugiperda larvae in three stages: low age（1st-2nd 
instar）, middle age（3rd-4th instar）and old age（5th-6th instar） in Hainan Island.【Result】The spatial distribution of S. 
frugiperda larvae was mainly related to corn planting system, larval instar and climate in Hainan. It was damaged seriously 
in seedling stage, small bell stage and big bell stage. The proportion of 3rd instar larvae was the highest, and they were 
distributed in aggregation. The 5th-6th instar larvae distributed evenly in flowering and mature stages. From the perspective 
of regional distribution, the S. frugiperda larvae were concentrated and distributed in the eastern and central parts of Hainan 
Province, and evenly distributed in the southern and northern parts（I>0, C>1, K>0, Cα>0, m*/m>1, α>0, β>1, lg α>1, 
b>1）. The data of each field were analyzed according to Taylor power law lg v=0.23663+1.24689 lg m（R=0.9437）and linear regression equation m*=-0.07870+2.16842m（R=0.7777）, and it could be concluded that the pests in each 
area of Hainan showed an aggregated distribution and the aggregation degree was not dependent on the population density.
【Conclusion】The research result will provide references for the investigation of population density and selection of 
appropriate period of pest control.]]></description>
<pubDate>2021/3/8 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[刘诗颖 1
，孙明凯 1
，李金花 1
，张业扬 1
，李　涛 2，陈剑山 2，范咏梅 1,2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>刘诗颖 1
，孙明凯 1
，李金花 1
，张业扬 1
，李　涛 2，陈剑山 2，范咏梅 1,2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202102014&flag=1]]></guid><cfi:id>41</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Behavior habits and Morphological characteristics of Polychrosis cunninhamiacola]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202102015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The trial was conclucted to understand the damage habits of the Polychrosis 
cunninhamiacola and identify the main morphological characteristics of male and female pupae and adults.【Method】The 
tops with larvae were collected from the Cunninghamia lanceolate forests and observed in insectarium combined with the 
observation outdoors. The behavior habits and morphological characters of larvae, male and female pupae and adults of P. 
cunninhamiacola were observed.【Result】The larvae of P. cunninhamiacola had shoot-transferring behavior habits and damaged the tops of C. lanceolata. When the larvae reach maturity, they pupated in the tip of a branch. Most pupae completed 
eclosion in the wee hours or in the morning, and the egg were scattered on the back of blades. The pupa was obtect and the new 
pupa was beige. The male pupae had a longitudinal genital cleft on the segmental venter of the ninth abdominal segment, with a 
hemispherical protuberance on each side. The distance between anal cleft and genital cleft was 80-170 μm. The genital cleft 
of female pupa was on the eighth abdominal segment and there was no hemispherical protuberance on either side of the cleft. 
The distance between anal cleft and genital cleft was 270-360 μm. The hapis at the tail of the male adult was obvious with 
forked or forceps shaped and densely scaly. The female adult had a kidney-shaped, orange ovipositor on its tail.【Conclusion】
The damage behaviors of P. cunninhamiacola（such as shoot-transferring）were understood, male and female pupae were 
identified by the the characteristics such as pupa’s abdominal segment and protuberance, and male and female adults were 
identified by adult’s hapis, ovipositor and other characteristics, which provided a theoretical basis for the monitoring and 
control of P. cunninhamiacola.]]></description>
<pubDate>2021/3/8 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[常明山1
，吴耀军1
，赵鹏飞 1
，蒋　华 2，周　通 3，张　旭 4，黄华艳 1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>常明山1
，吴耀军1
，赵鹏飞 1
，蒋　华 2，周　通 3，张　旭 4，黄华艳 1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202102015&flag=1]]></guid><cfi:id>40</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Establishment and Application of Fluorescent 
Recombinase-aided Amplification Method 
for Pantoea stewartii subsp. stewarii Detection]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202101014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was conducted to develop a rapid, sensitive and specific assay for detection of 
Pantoea stewartii subsp. stewarii based on fluorescent recombinase-aided amplification（RAA）.【Method】The pstSglmS sequence of P. stewartii subsp. stewarii was used as target sequence, and the specific primers and probes were designed 
and synthesized to establish a fluorescent RAA assay for rapid detection of P. stewartii subsp. stewarii. The sensitivity and 
specificity of the fluorescent method was detected. Simulated samples and actual samples were used to evaluate the reliability 
of fluorescent RAA, with the SN/T 1375-2004 as standard for verification.【Result】A fluorescent RAA for detection of P. 
stewartii subsp. stewarii was established, which could detect P. stewartii subsp. stewarii specifically at 39℃ within 20 min. 
The sensitivity of the fluorescent RAA was 280 fg/μL. With the established method, 6 simulated samples were positive and 
24 real samples were negative with the same results as SN/T 1375-2004.【Conclusion】The fluorescent RAA method for 
detection of P. stewartii subsp. stewarii is rapid, sensitive and specific, which is proved to be a great choice for site monitoring 
of P. stewartii subsp. stewarii.]]></description>
<pubDate>2021/1/26 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[SHAN Changlin, ZHOU Yuan, LI Xiaojun]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>SHAN Changlin, ZHOU Yuan, LI Xiaojun</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202101014&flag=1]]></guid><cfi:id>39</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Investigation and dot-ELISA Pathogen Identification of 
Virus Diseases in Pepper and Cherry Tomato in Hainan]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202101015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was conducted to investigate and identify the occurrence of virus diseases and virus 
types of peppers and cherry tomatoes in Hainan Province, and to determine the degree of damage and source of virus.【Method】
From 2018 to 2019, the symptoms, incidence rates and disease indexes of the viruses were analyzed for peppers and cherry 
tomatoes in 10 cities and counties of Hainan Province by 5 point sampling method. A total of 593 virus samples were collected 
from the main production areas of pepper and cherry tomato, to detect cucumber mosaic virus（CMV）, tobacco mosaic virus 
（TMV）, turnip mosaic virus （TuMV）, broad bean wilt virus （BBWV） and cucumber green mottle mosaic virus （CGMMV）by using the dot enzyme-linked immunosorbent assay （dot-ELISA）.【Result】The main symptoms of capsicum virus disease 
were characterized by mottled mosaic leaves and dwarf twigs, the incidence rate was 0-75%, and the disease index was 0-25.67. 
The main symptoms of cherry tomatoes were shrinkage and curling with mottled floral leaves. The incidence rate was 7%-98%, 
and the disease index was 1.67-37.33. The total detection rate of pepper virus was 37.5%, the compound infection rate was 2.08%, 
while the total detection rate of cherry tomato virus was 20.33%, and the compound infection rate was 0.98%.【Conclusion】Virus 
diseases are common in peppers and cherry tomatoes. The study results confirmed the disease status of pepper and cherry tomato 
in the field. The results of dot-ELISA showed that CMV and TMV were still the dominant virus sources among the five viruses 
detected, and there were four types of compound infection.]]></description>
<pubDate>2021/1/26 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[YAN Wanrong1, ZHAO Zhixiang1, XIAO Tongbin1, ZHANG Shanxue2, WEN Xiangling2, WANG Huifang1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>YAN Wanrong1, ZHAO Zhixiang1, XIAO Tongbin1, ZHANG Shanxue2, WEN Xiangling2, WANG Huifang1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202101015&flag=1]]></guid><cfi:id>38</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Identification and Biological Characteristics of Pathogen Causing Leaf Spot on Gladiolus hybridus]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202111011&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Leaf spot is an important factor restricting the gladiolus industry in Taishan City, Guangdong Province. To provide references for controlling the disease, the pathogen was classified and identified, and the biological characteristics and fungicide sensitivity were studied.【Method】The taxonomic status of pathogen was identified based on pathogenicity determination, morphological characteristics and rDNA-ITS sequence analysis. The effects of temperature, pH, light, carbon source, nitrogen source and fungicide on the pathogen were measured by mycelium growth method and microscopic examination.【Result】Four isolates were purified and obtained from the diseased samples with the same morphological characteristics. The results of pathogenicity determination showed that the symptoms produced by artificial inoculation were consistent with those observed in the field. Conidiophores of the pathogen were brown, mostly solitary, septate, and knee-curved at the top, with the size of 51.0 - 80.0 m×4.0 - 7.6 m. Conidia were brown, arched, four cells, wide in the middle and narrow at both ends, and curved to one side, with the size of 23.5 - 32.0 m×11.5 - 16.0 m. Phylogenetic analysis based on the rDNA-ITS sequences of the pathogen showed that the pathogen and Curvularia gladioli clustered together to form distinct branches. The pathogen was identified as C. gladioli Boerema & Hamers by morphological characteristics and rDNA-ITS sequence analysis. The optimal temperatures and pH ranges for mycelial growth and sporulation of the pathogen were 26 - 30 ℃ and 5.0 - 7.0, respectively. Light promoted the growth of mycelia and darkness promoted the sporulation. Carbon source xylose, glucose and organic nitrogen source beef extract were beneficial to the growth and spore production. Prochloraz and mancozeb had strongest inhibitory effect on mycelial growth of the pathogen, and EC50 were 1.23 g/mL and 2.81 g/mL, respectively.【Conclusion】The pathogen of Taishan gladiolus leaf spot disease was identified as C. gladioli, which was sensitive to prochloraz and mancozeb. And the optimal temperatures and pH ranges for the growth of the pathogen were 26 - 30 ℃ and pH 5.0 - 7.0, respectively.]]></description>
<pubDate>2021/12/13 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[沈会芳，张景欣，杨祁云，蒲小明，孙大元，林壁润]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>沈会芳，张景欣，杨祁云，蒲小明，孙大元，林壁润</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202111011&flag=1]]></guid><cfi:id>37</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effect of FOXO Gene on the Apoptosis of sf9 Cells Induced by Azadirachtin]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202111012&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study aimed to investigate the molecular mechanisms of azadirachtin（AZA）inducing the apoptosis of sf9 cells of Spodoptera frugiperda through FOXO transcription factor.【Method】The ovarian cells of culture in vitro S. frugiperda were divided into AZA group and control group. Cell proliferation and microcosmic changes of sf9 cells were analyzed using CCK-8 assay and transmission electron microscopy, respectively. Apoptotic cells stained by Annexin V-FITC/PI were separated with flow cytometry, and the expressions of p-FOXO and apoptosisrelated proteins were determined by Western blot. The expression of FOXO gene and the activity of Caspase-3 were detected by qRT-PCR and RNAi, respectively.【Result】The CCK-8 detection results showed that AZA inhibited the proliferation of sf9 cells significantly with a time and concentration dependent manner. Transmission electron microscopy revealed that 5 mmol/L AZA could obviously trigger changes in the microstructure of sf9 cells such as contraction of the nucleus, chromatin condensation and the development of cytoplasmic vacuoles. The results of flow cytometry revealed that AZA induced apoptosis of sf9 cells which was positively correlated with time. The results of Western blot analysis showed that AZA could significantly increase the expression of apoptosis-related proteins Bim and cleaved Caspase-3. Compared to the control group, the expression of P-FOXO protein was significantly decreased. However, the expression of the total FOXO protein remained apparently unaltered. The qRT-PCR expression analysis showed that the mRNA expression of FOXO transcription factor increased upon prolonged treatment with AZA. Silencing of FOXO in sf9 cells by RNAi interference caused a significant decline in the activity of Caspase-3 protein. 【Conclusion】Collectively, the results of the present study show that AZA can inhibit the proliferation of sf9 cells and promote the apoptosis by blocking the phosphorylation of FOXO gene.]]></description>
<pubDate>2021/12/13 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[邵雪花，赖　多，匡石滋]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>邵雪花，赖　多，匡石滋</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202111012&flag=1]]></guid><cfi:id>36</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effect of Low Temperature Stress on the Mortality of Dysmicoccus neobrevipes Beardsley]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202111013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The effect of low temperature stress on lethality of Dysmicoccus neobrevipes Beardsley（Hemiptera: Pseudococcidae）was determined.【Method】The lethality of D. neobrevipes at different development stages under different low temperatures（0 ℃ , 4 ℃ , 10 ℃）for five different time treatments（1 h, 2 h, 4 h, 6 h, 8 h）was observed in this study, with the compared temperature of 26 ℃ as control. And the effect of low temperature stress on the mortality of D. neobrevipes was analyzed.【Result】There were significant effects on the mortalities of the 1st and 2nd instar nymphs 
of D. neobrevipes under low temperature treatments of 0 ℃ , 4 ℃ and 10 ℃ , and the mortality in each treatment was more than 30%. The mortality of the 2nd instar nymph of D. neobrevipes was over 95% after being treated for 6 h and 8 h at the low temperature of 0 ℃ and 4 ℃ . There were no significant differences in the mortality of the 3rd instar nymph of D. neobrevipes after being treated for 1 h, 2 h and 4 h at 10 ℃ compared with that at 26 ℃ , with the mortality of less than 10%. For immature female adults of D. neobrevipes, there were no significant differences in the mortalities after being treated for 4 h, 6 h at 10 ℃ compared with those at 26 ℃ . For mature female adults, there were no significant differences in the mortalities of D. neobrevipes in different treatment times at 10 ℃（less than 3%）compared with those at 26 ℃ .【Conclusion】The results indicated that the low temperature stress had a significant effect on the mortality of the 1st and 2nd instar nymphs of D. neobrevipes, and the development stage of D. neobrevipes after the 3rd instar nymph could maintain a good survival ability at 10 ℃.]]></description>
<pubDate>2021/12/13 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[李德伟，吴建辉，覃振强]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>李德伟，吴建辉，覃振强</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202111013&flag=1]]></guid><cfi:id>35</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Identification of Peanut Pod Rot Pathogens in GuangdongProvince and Screening of Fungicides]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202208009&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was conducted to identify the pathogenic types of peanut pot rot in Guangdong 
and screen effective fungicides in order to provide a reference for the prevention and control of the disease in production.
【Method】The pure fungal cultures of fruit rot were isolated from the diseased peanut pods collected in fields by tissue 
isolation method, the pathogens were confirmed after the pathogenicity of the isolated strains were verified by back inoculation, 
and the pathogenic types were identified by cloning the strain’s ITS sequence and phylogenetic analysis. The tissue samples 
were used to isolate fungi from rotted peanut pods. The pathogenicity of all isolates were tested based on Koch’s rule, and then 
identified by ITS sequences analysis. The inhibition effect of 40% thiram WS, 98% hymexazol SP, 24% Jinggangmycin A AS, 
10% difenoconazole WDG and 10% polyoxins WP against the peanut pod rot pathogens were determined by mycelia growth rate 
method. The EC50 values of different fungicides were analysed.【Result】Two pathogenic fungi were isolated from the peanut 
pod rot samples collected in Shaoguan. Both of them could cause peanut pod rot and even kernel rot after back inoculation. 
Phylogenetic analysis based on ITS sequences showed that the pathogens belonged to Fusarium oxyspourm and Fusarium
solani. The inhibitory effect of 40% thiram WS was higher than other fungicides, and the EC50 value on F. oxyspourm and 
F. solani were 0.001 mg/L and 0.021 mg/L, respectively. The EC50 value of 98% hymexazol SP on two Fusairum strains were 
0.296 mg/L and 0.217 mg/L, respectively. The EC50 value of 24% Jinggangmycin A AS on two Fusairum strains were 20.575 mg/L 
and 11.185 mg/L, respectively. 10% difenoconazole WDG and 10% polyoxins WP had no inhibitory effect on the two Fusarium 
pathogen strains.【Conclusion】Peanut pod rot in Guangdon is mainly caused by Fusarium co-infection, and F. solani is the 
main pathogen. The two strains F. oxyspourm and F. solani are both sensitive to thiram and hymexazol, which can be used as 
fungicides for the prevention in production.]]></description>
<pubDate>2022/10/9 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[XING Juejun1
, XU Yuzhao2,3, SU Yutong2,3, WEI Xinying2,4, JIANG Haiqi2,3, YU Guohui2,3, YIN Yan5]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>XING Juejun1
, XU Yuzhao2,3, SU Yutong2,3, WEI Xinying2,4, JIANG Haiqi2,3, YU Guohui2,3, YIN Yan5</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202208009&flag=1]]></guid><cfi:id>34</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Susceptibility of Different Instar Larvae of Spodopterafrugiperda to Commonly Used Insecticides]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202208010&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study aims to understand the susceptibility variation of different instar larvae of 
Spodoptera frugiperda to commonly used insecticides, with a view to providing scientific basis for chemical control 
of this pest.【Method】The control efficacy of 10 insecticides to the larvae of the S. frugiperda was determined in the 
laboratory. The susceptibility of the first, third and fifth instar larvae to four insecticides with the control efficacy of over 
85% was compared by the bioassay method.【Result】The control efficacy of chlorantraniliprole, emamectin benzoate 
and chlorfenapyr to the third instar larvae of S. frugiperda was 100% seven days after treatment, while that of lufenuron 
was 91.67%, and that of cyantraniliprole was 88.89%. The remaining five insecticides had a low control efficacy, ranging 
from 44.44% to 69.44%. Bioassay results showed that the LC50 values of emamectin benzoate to the first, third and fifth instar larvae were 0.019, 0.0234, and 0.3918 mg/L respectively 48 hours after treatment. The LC50 of chlorantraniliprole to 
the first, third and fifth instar larvae were 0.2167, 0.2763, and 3.3718 mg/L 72 hours respectively after treatment, , while that 
of chlorfenapyr was 1.1286, 3.0194 and 19.2726 mg/L, and that of lufenuron was 1.2213, 2.1445 and 7.8419 mg/L. It could 
be seen that the susceptibility of S. frugiperda to insecticides was decreased with the increase of instar. The fifth instar larvae 
of S. frugiperda had significantly lower susceptibility to the four tested insecticides than the first and third instar larvae.
【Conclusion】The chlorantraniliprole, emamectin benzoate, chlorfenapyr and lufenuron were recommended for the control of 
first to third instar larvae of S. frugiperda. The emamectin benzoate and chlorantraniliprole were recommended to control fifth 
instar larvae.]]></description>
<pubDate>2022/10/9 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[CHEN Jincui1
, CAO Lijun1
, MA Zhongzheng1
, YUAN Xinxin2
, 
GONG Yajun1
, SHEN Xiujing1
, WEI Shujun1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>CHEN Jincui1
, CAO Lijun1
, MA Zhongzheng1
, YUAN Xinxin2
, 
GONG Yajun1
, SHEN Xiujing1
, WEI Shujun1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202208010&flag=1]]></guid><cfi:id>33</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Isolation, Identification and Biological Characteristics of An Endophytic Bacterium from Juncao“Oasis No.1”in Hainan]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202207011&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study aimed to explore the growth-promoting properties and antagonism of pathogenic 
fungi of an endophytic bacterium HLY3-2 isolated and identified from the leaves of Hainan Juncao“Oasis No.1”, with a 
view to providing basic theory for the biological control of plant pathogenic fungi and the study of plant probiotic mechanism.
【Method】The endophytic bacterium HLY3-2 was isolated from the leaves of Hainan Juncao “Oasis No.1” by using the 
dilution plating method. Through morphological identification,16SrDNA sequencing and homology analysis, a phylogenetic 
tree was constructed to identify the genus and species of this strain. The biological characteristics were explored by using the 
plate antagonism method and various physiology and biochemistry culture medium and growth-promoting property culture 
medium.【Result】HLY3-2 was identified as a strain of Bacillus thuringiensis through the endophytic bacterium morphology, 
physiology and biochemistry characteristics and 16SrDNA sequencing. The results of antagonism of pathogenic fungi showed 
that the strain HLY3-2 had some inhibition effect on Magnaporthe oryzae and Foctropical race4 with the bacteriostasis rates 
of 18.45% and 30.65% respectively. Then, according to results of the analysis on physiology and biochemistry characteristics, 
the strain did not have the ability of utilizing citrate, cellulose and starch. And it could not produce ester substances and 
hydrolyze gelatin during growth, but it had the ability of producing proteases and catalase during its growth and metabolism 
process. The results of growth-promoting property analysis showed that the strain had the ability to dissolve inorganic 
phosphorus and organophosphorus and degrade silicates, and could not produce iron carriers, but had strong IAA-producing 
ability.【Conclusion】The experiment results show that the endophytic bacterium HLY3-2 has a role in promoting plant growth and 
controlling disease.]]></description>
<pubDate>2022/9/5 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[LI Wanghao, ZHOU Ruipeng, XU Yuying，ZHAO Qiqi, LIU Juan, WANG Rongbo, YE Wenyu]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>LI Wanghao, ZHOU Ruipeng, XU Yuying，ZHAO Qiqi, LIU Juan, WANG Rongbo, YE Wenyu</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202207011&flag=1]]></guid><cfi:id>32</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Control Effects of Three Insecticide Sprays and Root Irrigation on Population of Tomato Bemisia tabaci]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202207012&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study aimed to explore the effects and application methods of dinotefuran, cyantraniliprole 
and spirotetramat in the control of Bemisia tabaci in Guangxi region, with a view to providing references for the control of B. tabaci in Guangxi. 【Method】The control effects of dinotefuran, cyantraniliprole and spirotetramat on B. tabaci population 
on tomatoes in open field and greenhouse were compared by spraying and root irrigation.【Result】In open field tomato 
experiment, after treatment for 3 to 21 days, there was no significant difference in adult and nymph population density of 
B. tabaci among different treatments, which was lower than 0.53 whiteflies per leaf. After being treated with cyantraniliprole 
spray for 42 days, the adult population density of B. tabaci was 0.63 whiteflies per leaf, which was significantly lower than of 
that of the treatment without spraying any insecticide (CK) (1.41whiteflies per leaf). There were significant differences in the 
population density of B. tabaci nymphs among different treatments after treatment for 28 to 42 days. The population density 
of B. tabaci nymphs treated with dinotefuran, cyantraniliprole and spirotetramat or root irrigation with 3 times the amount of 
cyantraniliprole was lower than 0.31 whiteflies per leaf after treatment for 28 to 42 days. In the greenhouse, the cyantraniliprole 
or spirotetramat had a good control effect on the adults and nymphs of B. tabaci, especially in the 28 d and 42 d after spraying, 
the population density of adults and nymphs was significantly lower than that of CK. The nymph densities of were 0.33 whiteflies 
per leaf and 0 whitefly per leaf after being treated cyantraniliprole and spirotetramat for 28 days, while the density of nymphs in 
CK was 2.85 whiteflies leaf.【Conclusion】Cyantraniliprole had a good and stable control effect on the population of B. tabaci. 
The spray treatment with dinotefuran showed a good effect on B. tabaci on tomatoes in open field, while the spray treatment 
with spirotetramat in greenhouse showed a good long-term effect on the control of B. tabaci. At the same dose, the control effect 
of cyantraniliprole and spirotetramat on B. tabaci population by spraying was generally better than that by root irrigation. The 
control effect of cyantraniliprole on B. tabaci population could be improved by increasing the amount of root irrigation.]]></description>
<pubDate>2022/9/5 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[LI Dewei, LUO Yawei, HUANG Zhenwen, LI Tao, LIANG Juju, XIE Wen, QIN Zhenqiang]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>LI Dewei, LUO Yawei, HUANG Zhenwen, LI Tao, LIANG Juju, XIE Wen, QIN Zhenqiang</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202207012&flag=1]]></guid><cfi:id>31</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Analysis on Land Carrying Capacity of Livestock and
Poultry Manure in Shanwei City]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202206011&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was conducted to investigate the status of land carrying capacity of livestock 
and poultry manure in Shanwei City, and to provide a theoretical basis for the combination of planting and livestock and poultry breeding and the rational development of breeding scales.【Method】According to the data in the Shanwei Statistical 
Yearbook, the nutrient supply of livestock and poultry manure, the demand of livestock and poultry manure nutrients for 
crops and the land carrying capacity of Shanwei City from 2017 to 2020 were analyzed.【Result】From 2017 to 2020, the 
livestock and poultry stocks in Shanwei City increased from 664 100 to 878 900 pig equivalents, and the breeding scales of 
Haifeng County and Lufeng City in 2020 increased greatly compared with those in the previous three years. The total nitrogen 
and phosphorus nutrient demand for crops in Shanwei City converted into livestock and poultry manure has increased from 
15 200 t and 3 600 t to 17 600 t and 4 100 t, respectively. During this period, the supply of nitrogen and phosphorus in livestock 
and poultry manure increased from 4 600 t and 800 t to 6 200 t and 1 100 t, respectively. The land carrying capacity indexes 
of livestock and poultry manure based on nitrogen and phosphorus in Shanwei were 0.30 - 0.35 and 0.22 - 0.26, respectively. 
【Conclusion】The overall scale of livestock and poultry breeding in Shanwei City still has great development potential. The 
land carrying capacity could be the basis for the agricultural departments to adjust the planning of the development of livestock 
and poultry breeding.]]></description>
<pubDate>2022/8/9 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[XIONG Zhenqian, DENG Dun, RONG Ting, SONG Min, LI Shuhong, YU Miao, MA Xianyong, LIU Zhichang]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>XIONG Zhenqian, DENG Dun, RONG Ting, SONG Min, LI Shuhong, YU Miao, MA Xianyong, LIU Zhichang</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202206011&flag=1]]></guid><cfi:id>30</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Evaluation of Land Ecological Security in Ecologically
Fragile Region of Shannan City Based on PSR Model]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202206012&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study evaluated the land ecological security in Shannan City of Tibet Autonomous Region, 
revealed the main factors affecting the land ecological security, with a view to providing reasonable suggestions for the 
realization of ecological environment protection and sustainable land use in Shannan City, and promoting the sustainable 
and healthy economic development on the premise of ensuring the land ecological security. 【Method】An evaluation 
index system of land ecological security was established based on Pressure-State-Response (PSR) Model to evaluate the 
state of land ecological security in the city from 2010 to 2020, and the main factors affecting land ecological security were 
identified by Obstacle Degree Model (ODM).【Result】From 2010 to 2016, the land ecological security index of Shannan 
City was between 0.41 and 0.50, showing a slight fluctuation. It continued to decrease from 2016 to 2018, and decreased to 
0.44. From 2018 to 2020, the land ecological security index increased rapidly from 0.44 to 0.69, which was closely related to the economic development and ecological environment protection strategies of Shannan City. Among the pressure, state and 
response indexes, the impact of pressure index on ecological security had little change, and its proportion in ecological security 
index increased from 29.19% in 2010 to 31.46% in 2020. The influence of state index decreased significantly from 41.62% 
in 2010 to 29.39% in 2020. The influence of response index increased significantly, from 29.19% in 2010 to 39.16% in 2020. 
The main influencing factors of land ecological security in Shannan City from 2010 to 2020 were per capita GDP, per capita 
rural disposable income, annual expenditure of environmental protection and fixed-asset investment.【Conclusion】In order 
to en sure the land ecological security of Shannan City, it is suggested to increase the annual expenditure of environmental 
protection and optimize the investment structure of fixed assets in a coordinated way while developing economy rapidly and 
increasing per capita GDP and rural per capita disposable income in the future.]]></description>
<pubDate>2022/8/9 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[LI Hongqing, YANG Yu, ZHANG Junhong, LI Wenqi]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>LI Hongqing, YANG Yu, ZHANG Junhong, LI Wenqi</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202206012&flag=1]]></guid><cfi:id>29</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Establishment of RT-LAMP-HNB Method for Detection of Cymbidium mosaic Virus and Odontoglossum Ringspot Virus]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202205011&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】There are more than 50 kinds of orchid viruses in the world, among which Cymbidium 
mosaic virus (CymMV) and Odontoglossum ringspot virus (ORSV) are the most common viruses. However, there are 
no effective measures to control the virus disease. Therefore, the establishment of efficient detection methods for the 
CymMV and ORSV is of great significance to control infected plants.【Method】CymMV and ORSV were used as 
experimental materials, and the reverse transcription loop-mediated isothermal amplification method (RT-LAMP) 
was used to amplify the viral RNA, so as to establish a rapid detection method of CymMV and ORSV. The RT-LAMP 
primers were designed according to the conserved regions of the cp gene sequences of two viruses. The hydroxylnaphthol blue (HNB, the final concentration of 200 μmol/L）was added to the RT-LAMP reaction system as an indicator of the 
amplification product. Without opening the tube for gel electrophoresis, the reaction results could be directly interpreted by 
the color change of the system which could avoid product aerosol pollution.【Result】The detection method could detect 
the presence of CymMV and ORSV RNA in the samples within 2 h, and its sensitivity was 10 times that of RT-PCR. In 
order to test the detection effect of RT-LAMP-HNB in actual production, 20 Phalaenopsis samples were detected by onestep RT-LAMP-HNB. According to the results, the detection rates of CymMV and ORSV were 70% and 55%, which 
were consistent with the results of RT-PCR, indicating that this method was suitable for the detection of field samples. 
【Conclusion】The one-step RT-LAMP-HNB established in this study is a sensitive, rapid, specific and convenient method 
for the detection of CymMV and ORSV. Nucleic acid amplification can be carried out only by simple temperature control 
equipment such as water bath pot, suitable for promotion and use in grass-roots or inconvenient areas.]]></description>
<pubDate>2022/7/11 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[XU Cong, HUANG Hao, LUO Huajian, DU Jiannan, HUANG Xiaoyan, CHEN Yan, LIANG Weiqu, HU Shan]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>XU Cong, HUANG Hao, LUO Huajian, DU Jiannan, HUANG Xiaoyan, CHEN Yan, LIANG Weiqu, HU Shan</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202205011&flag=1]]></guid><cfi:id>28</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[PMA-qPCR Detection System for Powdery Mildew of Rubber Tree and Its Application]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202205012&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Powdery mildew of rubber tree is the most serious leaf disease of rubber tree in China so far. 
The PMA-qPCR detection system for powdery mildew of rubber tree was constructed by combining propidium monoazide 
(PMA) with real-time fluorescence quantitative PCR (qPCR), which provided technical support for the timely and efficient 
detection of viable conidia of powdery mildew in rubber tree.【Method】Primers were designed according to the conserved 
region of ribosomal internal transcribed spacer (ITS) sequence of Erysiphe quercicola and its specificity was verified. 
The standard curve was drawn with the recombinant plasmid pMD-19T-Eq. A PMA-qPCR detection system for powdery 
mildew of rubber tree was established to evaluate its repeatability and sensitivity. The system was used to detect the viable 
count of powdery mildew after propiconazole treatment.【Result】The designed primers had good specificity. A good linear 
relationship between cycle threshold (Ct) and template concentration was found in the constructed qPCR standard curve for Erysiphe quercicola, the correlation coefficient was 0.9981, and the amplification efficiency was 107.97%. PMA-qPCR had 
little effect on the amplification of live spore suspension, and the detection sensitivity was 4.77×102
 copies/μL. After treatment 
with propiconazole for 15 min, the proportion of live bacteria decreased from 66.13% to 23.18%.【Conclusion】The PMAqPCR system could rapidly and quantitatively detect the number of live bacteria of powdery mildew in rubber tree, which had 
the advantages of sensitivity, accuracy and efficiency, and provided theoretical basis and technical means for the prediction and 
control of the disease.]]></description>
<pubDate>2022/7/11 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[GUAN Xin, TU Min, CAI Haibin, WANG Yunyue, ZENG Xia, HU Yanshi]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>GUAN Xin, TU Min, CAI Haibin, WANG Yunyue, ZENG Xia, HU Yanshi</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202205012&flag=1]]></guid><cfi:id>27</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effects of Host Switching on Diversity of Intestinal Bacteria in Batocera lineolata Larvae]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202204008&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was carried out to investigate the effect of host switching on the diversity 
of intestinal bacteria in Batocera lineolata larvae, and find out the host adaptability of B. lineolata larvae from the 
perspective of intestinal bacteria, with a view to providing a theoretical basis for the development and utilization of microbes. 【Method】B. lineolata larvae were raised with walnut as primary hosts and Eucalyptus as switching hosts, and the diversity 
of intestinal bacteria in B. lineolata larvae was studied by using the methods of traditional microbial culture, 16Sr RNA gene 
sequencing and high-throughput sequencing.【Result】A total of 19 culturable strains of bacteria were obtained through 
traditional microbial culture. 10 strains of bacteria were isolated from the intestine of the larvae of B. lineolata reared on the 
original host, and 9 strains were isolated from the larvae of B. reared by host; 6 genera of culturable bacteria were isolated from 
both treatments, 5 of which were common genera. High-throughput sequencing results showed that there were no significant 
differences in the diversity of intestinal bacteria in the larvae before and after host switching. B. lineolata larvae intestinal 
bacteria samples respectively belonged to 20 phylums, 32 classes, 55 orders, 90 families and 170 genera, including, under two 
treatments, both B.lineolata larvae gut dominant fungi belonging to the Proteobacteri, Gammaproteobacteria, Enterobacteriales, 
Enterobacteriaceae and Uncultured bacterium f Enterobacteriaceae.【Conclusion】The composition of intestinal bacteria 
species of B. lineolata larvae under two treatments was similar, the overall diversity of intestinal bacterial community was 
relatively rich and stable, and it was difficult to change the diversity of intestinal bacteria by changing the host in a short period 
of time.]]></description>
<pubDate>2022/6/6 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[XU Lei, CHEN Naiming, YANG Zhende, ZHOU Hao, LI Qiong, WEI Shanfang]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>XU Lei, CHEN Naiming, YANG Zhende, ZHOU Hao, LI Qiong, WEI Shanfang</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202204008&flag=1]]></guid><cfi:id>26</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Analysis of Monitoring Result of Farmland Rodents inNanxiong City and Xinhui District, Guangdong Province]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202204009&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was carried out to understand the rodent infestation in typical farmlands and 
the difference of rodent community structure in different habitats of Guangdong Province, with a view to providing basis for the prevention and control of rodents in Guangdong. 【Method】Night trapping method was used to monitor rodents for 
three consecutive years at two monitoring sites: Nanxiong City in northern Guangdong and Xinhui District in Pearl River Delta. 
Quantitative analysis of the rodent species composition, population size and community biomass structure was conducted. In 
addition, the differences of rodent community structures in different habitats were compared.【Result】The total captured 
rate in 3 years was 6.37%. A total of 836 captured individuals belonged to 5 species of rodents. Both captured number (589 
individuals) and biomass (322.34 g/100 trap nights) of Rattus losea were dominant in those 5 species. The basic species 
composition of rodent community in farmlands of monitoring sites was“Rattus losea + Bandicota indica + Mus caroli + Rattus 
norvegicus + Rattus tanezumi”. The monthly dynamics of rodent population showed a bimodal pattern, with two peaks (in 
January and November) and two valleys (in April and July) every year. The highest rodent population density season was winter, 
and the average captured rate was 7.17 (±2.49)%. While the spring was the lowest rodent population density season with the 
average captured rate of 4.86 (±2.21)%. The rodent population density and biomass at Nanxiong monitoring site (8.38%±5.05%, 
727.92 g/100 trap nights) were significantly higher than those at Xinhui monitoring site (4.88%±4.28%, 472.52 g/100 trap 
nights). There also were significant differences in species composition, population size and community biomass structure 
between the two monitoring sites.【Conclusion】There are significant differences in farmland rodent population size in different 
months, different seasons, and at different sites. To achieve sustainable rodent pest management under the new circumstances, 
it is needed to enhance the accuracy of monitoring and early warning, and to constantly adjust the control strategies according to 
the changing planting structure and habitat conditions.]]></description>
<pubDate>2022/6/6 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[LIN Siliang,
　HUANG Lisheng, LAN Zisheng, LAI Rongfeng, YAO Dandan, JIANG Hongxue, FENG Zhiyong]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>LIN Siliang,
　HUANG Lisheng, LAN Zisheng, LAI Rongfeng, YAO Dandan, JIANG Hongxue, FENG Zhiyong</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202204009&flag=1]]></guid><cfi:id>25</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Screening and Identification of Antagonistic Endophytic Bacteria Against Phytophthora drechsleri in cucumber]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202204010&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Endophytic bacteria with good resistance to Phytophthora were screened from cucumber 
leaves and identified, so as to provide experimental materials for further study of theoretical exploration and their application 
in controlling cucumber blight.【Method】Endophytic bacteria were screened from cucumber leaves, and were preliminarily 
screened according to microscopic characteristics, Gram staining reaction, colony arrangement and colony morphology. 
The antagonism of endophytic bacteria was tested by plate antagonism method and fermentation liquor antagonism method. 
Through 16S rDNA sequencing and homology analysis, phylogenetic tree was constructed to identify the target strains.
【Result】Thirty-two endophytic bacteria were preliminarily screened and numbered as CL1-CL32, including 10 strains of 
Gram-negative bacillus, 13 strains of Gram-positive bacillus and 9 strains of Gram-positive cocci. Eight strains were found 
to have strong inhibitory effect on Phytophthora drechsleri by plate confrontation method, and the inhibition rate of mycelia ranged from 10.3% to 54.3%. The inhibition radius ranged from 1 mm to 7 mm. Three strains (CL7, CL9, CL15) with better 
inhibitory effect were tested in fermentation liquor antagonism experiment, and the inhibition rates were 54.4%, 72.2%, 63.3%, 
respectively.【Conclusion】The three endophytic bacteria (CL7,CL9,CL15) with strong inhibitory effect on P. drechsleri
belonged to Pantoea sp., Pseudomonas sp.,and Staphylococcus sp., among which CL9 belonging to Pseudomonas sp. had the 
most obvious inhibitory effect.]]></description>
<pubDate>2022/6/6 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[ZHENG Jing, LIANG Lexia, XUAN Qianqian, GUO Huiying, ZHOU Zhiguo, SUN Zemin, ZUO Guocai]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>ZHENG Jing, LIANG Lexia, XUAN Qianqian, GUO Huiying, ZHOU Zhiguo, SUN Zemin, ZUO Guocai</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202204010&flag=1]]></guid><cfi:id>24</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Study on Volatile Components and Antibacterial Activitiesof Hydrosols from Cinnamomum camphora Leaves]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202204011&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The volatile components and antibacterial activities of hydrosols from Cinnamomum 
camphora leaves were studied in order to provide references for the use of hydrosols as natural preservatives and disinfection 
products.【Method】Taking leaves of linalool, citral, cineol and borneol type camphor tree 〔Cinnamomum camphora (L.) 
Presl〕 as research objects, the hydrosols were extracted by steam distillation, and the volatile components, physicochemical 
properties and antibacterial effects of hydrosols were discussed.【Result】The main component in hydrosol of linalool type 
camphor leaf was linalool, with a relative mass of 74.03%; the main components of hydrosol in citral type camphor leaf were 
neroli and geranial, with a relative mass of 14.23% and 29.26%, respectively; the main component in hydrosol of cineol 
type camphor leaf was 1,8-cineole, with a relative mass of 50.36%; and the main component in hydrosol of borneol type camphor leaf was borneol, with a relative mass of 82.27%. Hydrosol of linalool camphor leaf had the best antibacterial effect, 
and its minimum inhibitory concentration (MIC) for all bacteria and fungi was 250 μL/mL, followed by hydrosols of cineol and 
citral camphor leaves with d better antibacterial effect. Hydrosol of cineol type camphor leaf had the best bactericidal effect, the 
minimum bactericidal concentration (MBC) against all bacteria and fungi was 250 μL/mL, followed by hydrosols of borneol type 
and linalool type camphor leaves.【Conclusion】Hydrosol of linalool type leaf had the best antibacterial effect, followed by that 
of citral and cineol type. Hydrosol of cineol type leaf had the best bactericidal effect, followed by that of borneol and linalool 
type.]]></description>
<pubDate>2022/6/6 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[YANG Suhua, ZENG Xianbiao, DENG Yuyin, LU Shunzhong, LI Guiqing, SU Lihua, DANG Zhongguang]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>YANG Suhua, ZENG Xianbiao, DENG Yuyin, LU Shunzhong, LI Guiqing, SU Lihua, DANG Zhongguang</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202204011&flag=1]]></guid><cfi:id>23</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Identification and Fungicides Screening of Lasiodiplodiatheobromae Causing Leaf Spot of Agave sisalana]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202202011&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Agave sisalana is an important hemp crop in China. A kind of leaf spot of sisal disease 
occurs in Zhanjiang area of Guangdong Province, which can lead to a large area of necrosis of sisal leaves and affect the 
yield. The identification and fungicide test of the pathogen causing leaf spot of sisal were conducted so as to control the 
disease effectively.【Method】The fungi were isolated from diseased sisal on PDA medium by tissue isolation method, 
and the pathogen was obtained after the validation of Koch’ s rule. The pathogen was identified with the morphological 
characteristics of the fungus and its ITS (internal transcribed spacer) sequence. Furthermore, the sensibilities of different 
fungicides to the pathogen were determined by mycelial growth rate method.【Result】It was found that the conidia, 
isolated from the leaf spot of sisal in Zhangjiang area, were oval, single spore, dark brown, separated by a middle septum, 
which was similar to the combination of two cells, and the ITS sequence had more than 99% of homology with Lasiodiplodia 
theobromae. The fungicides results showed that chlorothalonil, hexaconazole, prochloraz, tebuconazole, difenoconazole, fipronamide and thiophanate methyl all had good inhibition effect on Lasiodiplodia theobromae, among which chlorothalonil 
had the best inhibition effect with EC50 value of 0.000132 μg/mL.【Conclusion】The pathogen isolated from leaf spot of sisal 
in Zhanjiang area was identified as L. theobromae, which was sensitive to chlorothalonil, hexazolol, imitamine, tebutazolol, 
benzoethycloazole, flupyramide and methyl thiobacillam.]]></description>
<pubDate>2022/3/29 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[GUAN Tianbo，CUI Xiaodong, CHEN Kaihong, LIU Qiongguang]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>GUAN Tianbo，CUI Xiaodong, CHEN Kaihong, LIU Qiongguang</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202202011&flag=1]]></guid><cfi:id>22</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Method for Rapid Detection of Fusarium oxysporum andPhytophthora parasitica var. nicotianae in Tabacco Planting Soil]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202202012&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Fusarium wilt and tobacco black shank, caused by Fusarium oxysporum and Phytophthora 
parasitica var. Nicotianae, are common soil-borne diseases in tobacco planting soil, leading to severe damages. Therefore, 
a rapid detection method for the two pathogens was established to provide technical support for disease epidemic and 
treatment in the process of tobacco planting and ensure the stable production of tobacco industry.【Method】Taking the 
soil with soil-borne disease and healthy soil in Baise area of Guangxi were used as test materials, and the standard curve 
was established with the standard pathogen as reference template. The primers were designed by the conservative regions of the two pathogens, and the specific primers were screened according to the target bands and the dissolution curve and Ct value 
of fluorescence quantitative PCR. At the same time, the extraction method of DNA kit of microbial in soil was improved, and the 
detection systems of PCR and qPCR were established.【Result】A pair of specific primers PN3 and JBR for F. oxysporum and 
P. parasitica var. nicotianae were screened respectively, and the template concentration ranged from 1×102
 to 1×10-4 ng, 
with good linear relationship. The number of two pathogens in diseased soil was higher than that in healthy soil. The method of 
combination of soil drying, grinding and eluting for twice was helpful to extract the DNA of pathogens in the soil. A qPCR detection 
method with the products obtained by ordinary PCR pre-amplification reaction as a template was established.【Conclusion】The 
combination of PCR and qPCR can quickly distinguish and detect F. oxysporum and P. parasitica var. nicotianae, which plays an 
important role in real-time monitoring and early warning of fusarium wilt and tobacco black shank.]]></description>
<pubDate>2022/3/29 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[ZHANG Jili, QI Shi, LUAN Xinbo, JIN Yabo, HUANG Chongjun, LI Ping, WEI Jianyu, YAN Jian]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>ZHANG Jili, QI Shi, LUAN Xinbo, JIN Yabo, HUANG Chongjun, LI Ping, WEI Jianyu, YAN Jian</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202202012&flag=1]]></guid><cfi:id>21</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Complete Genome Sequence Analysis of Guangdong Isolates of Cymbidium Mosaic Virus]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202202013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Cymbidium mosaic virus (CymMV) is one of the most important orchid viruses. The wide 
spread of CymMV poses a serious threat to the development of orchid industry. The study is conducted to explore the genetic 
information and evolution of CymMV, which will provide an important scientific basis for monitoring and early warning of 
orchid virus disease and genetic engineering of orchid virus disease in Guangdong Province. 【Method】RT-PCR and DASELISA were used to detect and identify CymMV from leaves with suspected virus disease of Cymbidium sinense collected 
from Guangzhou area. The genome sequence assembly, annotation, phylogeny and selection p ressure analysis of CymMV 
isolates were performed with related molecular biology software.【Result】Two CymMV isolates (GZV013 and ZC29) were 
found in Guangdong Province for the first time in this study. Both of the genome lengths of GZV013 and ZC29 were 6 227 nt, encoding five functional proteins. The similarity analysis of the full sequence showed that the nucleotide sequence identity 
of GZV013 and Taiwan isolate M2 was 97.03% and that of ZC29 and Nanjing isolate NJ-1 was 97.11%. The complete genome 
sequence identity among CymMV isolates ranged from 86.85% to 98.31%, and the differentiation of diverse populations was 
closely related to host species and geographical isolation. Each region of CymMV genome was affected by negative selection 
and conformed to the neutral evolution model. The RdRp, TGB1 and TGB2 genes exhibited the highest genome-wide diversity. 
【Conclusion】The GZV013 was most closely related to Taiwan isolate M2, and ZC29 was most closely related to Nanjing 
isolate NJ-1, belonging to the same branch of a family.]]></description>
<pubDate>2022/3/29 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[WEI Yonglu, REN Rui, GAO Jie, LIU Weiping, XIE Qi, ZHU Genfa, YANG Fengxi]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>WEI Yonglu, REN Rui, GAO Jie, LIU Weiping, XIE Qi, ZHU Genfa, YANG Fengxi</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202202013&flag=1]]></guid><cfi:id>20</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Analysis of SSR and SNP Loci in Agrotis segetum Based on Transcriptome Sequencing]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202202014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Based on transcriptome dataset of Agrotis segetum, the SSR and SNP loci were analyzed．
【Method】Total RNA of A. segetum was extracted and the transcriptomes were sequenced with Illumina sequencing 
platform. SSR and SNP information in total unigene sequences were separately analyzed by MISA and GATK.【Result】
In the study, the A. segetum tra nscriptome database contained 66 469 unigene sequences. MISA was used to search the 
Unigene sequences and 4 438 SSR loci were obtained, which were distributed on 4 048 Unigene sequences, accounting 
for 6.09% of the total sequences. Mononucleotide and trinucleotide repeats accounted for 54.73% and 29.29% of the total 
repeats, respectively. The A/T motifs were the dominant microsatellite loci of A. segetum SSR. SSR motifs contained 4 to 
24 repeats, with 5 repetitions (21.67%) as the dominant repetitions. The length of SSR loci sequence ranged from 12 bp 
to 127 bp, including 3 493 moderately polymorphic loci and 820 highly polymorphic loci. GATK was used to search SNP loci in Unigene sequence, and 371 148 SNP loci were successfully searched, inculding 237 619 conversion types and 133 
529 transversion types. C/T accounted for 18.61% of the total SNP sites, with the highest ratio, followed by G/A accounting 
for 18.28%, and all of which belonged to conversion types. The ratio of conversion type (64.02%) was significantly higher 
than that of transversion type (35.98%).【Conclusion】The SSR loci in the transcriptome database of A. segetum have higher 
distribution frequency, more species and richer polymorphism; and the conversion type is the main type of SNP variation, 
which can provide a very important scientific basis for future studies on the genetic structure and differentiation, genetic 
evolution, migratory rule and integrated control of A. segetum population.]]></description>
<pubDate>2022/3/29 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[CHANG Hong, GAO Yan, WANG Siwei, WANG Xiaonan, LIU Yanping, SUN Haibin]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>CHANG Hong, GAO Yan, WANG Siwei, WANG Xiaonan, LIU Yanping, SUN Haibin</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202202014&flag=1]]></guid><cfi:id>19</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Analysis of Colonization Ability of Six Biocontrol Bacteria in Cucumber Plants under Chemical Stress Condition]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202202015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was carried out to explore the effect of chemical stress on the colonization ability of 
biocontrol bacteria on cucumber plants.【Method】By setting a greenhouse experiment and two field experiments, biocontrol 
bacteria were inoculated on cucumber plants without fungicides and pathogens, with fungicides and without pathogens, with fungicides and pathogens, respectively.【Result】The six biocontrol strains tested had good colonization effects on cucumber 
leaves and roots. Under the greenhouse conditions without fungicides and pathogens, the colonization of each biocontrol strain 
showed a trend of decrease-increase-stability, strains 1BS4 and 3BY4 had strong colonization ability in cucumber leaves and 
roots. The colonization quantity of strains 1BS4 and 3BY4 was close to 106
 CFU/g(FW) at 30 days post inoculation(dpi) about 104
CFU/g(FW); Under the field conditions without fungicides and with pathogens, there were significant differences in the colonization 
quantities of various biocontrol strains in leaves and roots, and they all showed a trend of decrease-increase-decrease-stability. 
Strain 5BJN1 had the strongest colonization ability. The colonization quantity of cucumber leaves and roots at 30 dpi was 105.92
CFU/g(FW) and 104.95 CFU/g(FW), respectively; Under the field conditions with both fungicides and pathogens, there were 
differences in the colonization quantity of biocontrol bacteria in leaves and roots, but the difference was not significant, and all 
showed a trend of decrease-increase-decrease-stability, and the colonization quantities of strains 3BJN7 and 5BJN1 in cucumber 
leaves at 30 dpi were relatively high, which were 103.88 CFU/g(FW) and 104.23 CFU/g(FW), respectively. The colonization quantity 
of other strains was about 103
 CFU/g(FW).【Conclusion】The effect of chemicals on the colonization ability of biocontrol bacteria 
depends on the types of strains. Some strains (3BJN7 and 5BJN1) have good compatibility with chemicals. In the presence of 
cucumber downy mildew, they can still maintain a certain amount of colonization after using chemicals, therefore, they can be 
used as potential biocontrol agents; In the absence of fungicide stress, the emergence of downy mildew pathogen can induce the 
improvement of colonization ability of strain 3BJN7 so that it can colonize stably on cucumber plants.]]></description>
<pubDate>2022/3/29 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[ZHENG Li, HUANG Jiehao, LIU Hongxia, LUO Yuming, GUO Jianhua]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>ZHENG Li, HUANG Jiehao, LIU Hongxia, LUO Yuming, GUO Jianhua</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202202015&flag=1]]></guid><cfi:id>18</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Research Progress of Insect Odorant Receptors]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202201013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[For insects, olfaction is an important signal input source for their behavioral perception. There are 
many kinds of proteins involved in the process of odor recognition, and they play a leading role in regulating feeding, 
swarming, mating, oviposition and other behaviors of insects. These proteins include Odorant-binding Proteins (OBPs), 
Chemosensory Proteins (CSPs), Odorant Receptors (ORs), Ionotropic Receptors (IRs), Sensory Neuron Membrane Proteins 
(SNMPs) and Odorant Degrading Enzyme (ODE). Among them, ORs play a critical role in identifying odor molecules and 
transmitting olfactory signals downstream. The specific combination of odorant molecule and dendrites of olfactory sensory 
neurons (OSNs) mediated by odorant receptors is an important basis for olfactory recognition. Odorant receptors involves two types: conventional odorant receptors (ORs), and odorant receptor coreceptor (ORCO). ORs, a highly divergent family of 
receptors, have the ability to recognize odor molecule, and the homology is lower between different insects. ORCO, a receptor 
without odor sensitivity, co-expresses with ORs to recognize odor molecule, and its sequence is remarkably conserved among 
insect species. Cell line culture, voltage clamp technique and drosophila“empty neuron”method were used to verify the 
function of odor receptors. Research about odor receptors’function can lay a theoretical foundation for looking for odor 
ligands, development of food attractants and repellents against pests, sex attractants and aggregation pheromones and so 
on. This article reviews the research progress in identification and classification, structure, expression profile, phylogenetic 
relationships and function of odorant receptors in the domestic and overseas, which provides reference for the research of 
insect olfactory mechanism as well as the prevention and control of pests.]]></description>
<pubDate>2022/2/25 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[QI Quanmei, LI Qiurong]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>QI Quanmei, LI Qiurong</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202201013&flag=1]]></guid><cfi:id>17</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Molecular Identification of Anthracnose Pathogen of Chrysanthemum in Huaiji, Guangdong Province]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202201010&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was conducted to identify the pathogen causing anthracnose of Chrysanthemum
in Huaiji of Guangdong province, and to screen the best control agents in the field in order to provide guidance 
for the prevention and control of Chrysanthemum anthracnose.【Method】Diseased samples (flowers and leaves) 
of Chrysanthemum with anthracnose symptoms collected from Huaiji of Guangdong were isolated and cultured 
conventionally. Fungal strains Cg1-1, Cg1-2 and Cg1-3 were isolated and obtained. The pathogenicity of above 
strains were determined by the Koch’s postulates with in vivo and in vitro inoculation of conidial suspension on plants 
of Chrysanthemum. The spores sizes and myelium morphologies of Cg1-1, Cg1-2 and Cg1-3 were observed under 
microscope and polygene molecular identification was conducted with ribosomal transcription spacer (ITS), large subunit (LSU)、Chitin synthase (CHS1) and actin (ACT). Finally, Mega7.0.18 was used to construct the phylogenetic tree of Cg1-
1, Cg1-2 and Cg1-3 with the maximum natural method. Difenoconazole, Prochloraz, 30% Thifluzamide (Liangzun), 430 g/L 
Tebuconazole (Cuijian) and 40% Thifluzamide·Trifloxystrobin (Runxiu) were used to control Chrysanthemum anthracnose 
in the field.【Result】Through the analysis of the biological characteristics of pathogen and the identification of polygene 
molecular biology, the anthracnose pathogen on Chrysanthemum in Huaiji of Guangdong Province is Colletotrichum 
gloeosporioides. The field efficacy test results showed that 1 500 times Difenoconazole and 1 500 times Prochloraz has the 
best control effects on the disease in the field. Attention should be paid to effective application of agents after the rain and 
in the rapid growing season of Chrysanthemum.【Conclusion】C. gloeosporioides is the causative agent of anthracnose 
on Chrysanthemum in Huaiji of Guangdong. 1 500 times Difenoconazole and 1 500 times Prochloraz can be used as 
recommended agents for field control.]]></description>
<pubDate>2022/2/25 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[CUI Yiping，LI Yifang，PENG Aitian，SONG Xiaobing，LING Jinfeng，HUANG Feng，CHEN Xia]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>CUI Yiping，LI Yifang，PENG Aitian，SONG Xiaobing，LING Jinfeng，HUANG Feng，CHEN Xia</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202201010&flag=1]]></guid><cfi:id>16</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[A Preliminary Study on the Effects of Combination of Biocontrol Agents Against Watermelon Fusarium Wilt Caused by Fusarium oxysporum f. sp. niveum]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202201011&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was carried out to screen ef fective combinations of biocontrol agents against 
wa termelon Fusarium wilt caused by Fusarium oxysporum f. sp. niveum【Method】Five biocontrol agents including 
23（Bacillus amyloliquefaciens）, 25（Exiguobacterium acetylicum）, 28（Bacillus pumilus）, 37（Bacillus 
licheniformis）and 156（Bacillus cereus）were combined to obtain 31 combinations. The plate bacteriostasis effects of 
biocontrol agents and volatile organic compounds（VOCs）produced by each combination were studied via dual-culture 
test and separation plate, then the effect of each combination on the germination rate of watermelon seeds and the growth 
of seedlings as well as the biocontrol efficacy of these combinations against watermelon Fusarium wilt were determined.
【Result】The 31 combinations showed significant inhibition effect on FON growth, among which the inhibition rate of C28 
was 68.06%, while the VOCs produced by C13（28+37）was 41.60%. There was no significant difference in the germination 
rates of watermelon seeds pre-treated by various combinations and CK group, however, the gemination rate of seeds and 
emergence rate of cotyledons presented a decreasing trended with the increase of the number of single isolates. In the pot 
test, there were differences in the control effects of various combinations against watermelon Fusarium wilt, and the C22 had 
the highest control efficacy of 61.1%.【Conclusion】There were differences in the plate antagonism and pot control effects 
of 31 biocontrol combinations against watermelon Fusarium wilt, and it was predicted that the combination formed by two or 
three single isolates had the best effect, and the antagonistic combinations are more beneficial to disease control.]]></description>
<pubDate>2022/2/25 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[LAN Jianrong, HUANG Jiehao, HUANG Shilian, Li Jinxuan, Tian Kai, LIU Qing, WU Jianxiong, ZHENG Li]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>LAN Jianrong, HUANG Jiehao, HUANG Shilian, Li Jinxuan, Tian Kai, LIU Qing, WU Jianxiong, ZHENG Li</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202201011&flag=1]]></guid><cfi:id>15</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Evaluation of the Effect of Dry Heat Treatment on Black Rot in Chinese Cabbage Seeds]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202201012&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was carried out to explore the effects of different dry heat treatment conditions 
on seed-borne black rot (Xanthomonas campestris pv. campestris) in Chinese cabbage seeds.【Method】Different heat 
combinations of temperature and time groups were designed. Further, different heat treatments on the inhibition effect 
of pathogenic bacteria, the sterilization effect of seed-borne pathogens and seed vigor were evaluated.【Result】The 
conditions for inactivation of black rot bacteria in vitro pathogens were selected as follows: continuous heat treatment at 
45 ℃ for 4 h or above; of continuous heat treatment at 50 ℃ or 60 ℃ or 65 ℃ for 0.5 h or above. The inhibition rate of 
pathogenic bacteria in infected seeds was 100% and the seed vigor of the infected seeds could be improved when treated 
at 50 ℃ for 8 h.【Conclusion】Continuous heat treatment at 50 ℃ for 8 h was the safe and effective dry heat treatment 
conditions for seed-borne disease black rot in Chinese cabbage seeds, which could provide a technical reference for the 
control of vegetable seeds borne disease.]]></description>
<pubDate>2022/2/25 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[YUE Xinlu , LIANG Gen, LI Zhiqiang, LI Pingdong, LIU Zhen, PENG Liya, YANG Wanqiong, HU Maolin]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>YUE Xinlu , LIANG Gen, LI Zhiqiang, LI Pingdong, LIU Zhen, PENG Liya, YANG Wanqiong, HU Maolin</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202201012&flag=1]]></guid><cfi:id>14</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Molecular Identification of Pathogens Causing Anthracnose on Shatian Lemon in Guangzhou City]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202212011&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study aimed to clarify the types of pathogens causing anthracnose on Shatian lemon, with a view to providing guidance for the prevention and control of anthracnose on Shatian lemon in field.【Method】The collected anthracnose samples were isolated and purified, and 9 strains of the pathogens ST2-1, ST2-2, ST2-3, ST3-1, ST3-2, ST3-3, ST4-1, ST4-1, ST4-2,and ST4-3 of anthracnose on Shatian lemon were obtained. Then puncturing and spraying inoculation methods were used to test the pathogenicity of the above strains, and their ITS, TUB2, ACT, LSU, GAPDH and CAL fragments were cloned for multi-gene joint identification.【Result】ST2-1, ST2-2, ST2-3, ST3-1, ST3-2, ST3-3, ST4-1,ST4-2 and ST4-3 are all highly pathogenic. The biological characteristics and phylogenetic tree indicated that ST2-1, ST2-2 and ST2-3 belonged to Colletotrichum siamense, ST3-1, ST3-2 and ST3-3 belonged to Colletotrichum cliviicola, ST4-1, ST4-2 and ST4-3 belonged to Colletotrichum fructicola.【Conclusion】Based on the morphological characteristics and molecular biological identification results, the pathogens causing anthracnose on Shatian lemon in Guangzhou were C. siamense, C.cliviicola and C. fructicola, in which C. siamese was isolated from the diseased fruit of Shatian lemon, C. cliviicola and C. fructicola were isolated from the diseased leaves of Shatian lemon, and it was the first report in the world that C. cliviicola could cause anthracnose on citrus plants.]]></description>
<pubDate>2023/2/6 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[CUI Yiping1, PENG Aitian1, SONG Xiaobing1, FENG Guanjie2, HUANG Feng1,CHEN Xia1, LING Jinfeng1, CHENG Baoping1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>CUI Yiping1, PENG Aitian1, SONG Xiaobing1, FENG Guanjie2, HUANG Feng1,CHEN Xia1, LING Jinfeng1, CHENG Baoping1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202212011&flag=1]]></guid><cfi:id>13</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Molecular Identification and Genetic and Evolutionary Analysis of Sweet Potato Leaf Curl Virus Infecting Ipomoea purpurea (L.) in Hubei]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202210011&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study is conducted to identify the viral pathogen of Ipomoea purpurea (L.) with yellow 
vein in Jingzhou, Hubei Province, and the results will provide theoretical basis for the effective prevention and control of this disease.【Method】One I. purpurea (L.) sample with yellow vein and curly leaves was collected from Jingzhou, Hubei 
Province. The sample was detected by PCR with a pair of begomovirus degenerate primers PA and PB. Gene cloning, nucleotide 
sequence comparison and phylogentic tree construction were applied for the comparison and genetic and evolutionary analysis 
of the obtained full-length genome sequence. 【Result】PCR results showed that a PCR amplicon with an expect size of 363 
bp were amplified from the samples, which confirmed that the collected I. purpurea (L.) were infected by begomoviruses. A fulllength viral genome sequence of 2 827 bp was obtained from the positive samples by segmenting cloning. Nucleotide similarity 
comparison showed that the sequence in this study had more than 91% nucleotide identities with other isolates of sweet potato 
leaf curl virus (SPLCV) available in GenBank, and it had 97.52% and 96.81% nucleotide identities with Hunan isolate (GenBank 
accession number: KY783941) and Jiangsu isolate (GenBank accession number: FJ176701), respectively. According to the 
standards of the International Committee on Taxonomy of Viruses for the classification of begomoviruses, begomoviruses with 
nucleotide identities of over 91% were classified as the same virus, and the begomovirus infecting I. purpurea (L.) was identified 
as SPLCV. Phylogenetic tree analysis showed that the SPLCV Hubei isolate (GenBank Accession number: OM287440) were 
clustered in a big branch with the SPLCV isolates from China and other Asian areas, and clustered with Hunan isolate in a 
small branch, indicating their close evolutionary relationship.【Conclusion】SPLCV is the pathogen that infects I. purpurea
(L.) in Hubei, and the disease is probably transmitted from Hunan through seedlings or Bemisia tabaci. This is the first report of 
SPLCV infecting I. purpurea (L.) in Hubei.]]></description>
<pubDate>2022/11/29 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[LUO Wandi1, 2，WANG Peng1
，MO Cuiping2
，CAI Jianhe2
，ZHANG Songbai1
，LI Zhanbiao2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>LUO Wandi1, 2，WANG Peng1
，MO Cuiping2
，CAI Jianhe2
，ZHANG Songbai1
，LI Zhanbiao2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202210011&flag=1]]></guid><cfi:id>12</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Antifungal Effects of Seven Biological Fungicides Against the Pathogen Causing Stem Rot Disease in Passion Fruits]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202210012&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】In order to screen safe and effective fungicides to provide guidance for passion fruit 
production, seven biological fungicides were used as materials to study their control effects on the pathogen causing stem rot 
disease in passion fruits.【Method】Seven botanical and microbiological fungicides were used to determine their toxicity 
effects on the pathogen causing stem rot disease in passion fruits by mycelium growth rate method, the effects of fungicides 
on mycelium growth and spore morphology were observed, and their control effects were tested by in vivo inoculation method.
【Result】All the selected fungicides showed inhibition effects on mycelium growth but had different effects on spore growth 
and morphology. Based on the indoor toxicity test results, among the botanical fungicides, Jinggangmycin showed the best inhibition effect with an EC50 of 7.79 mg/L, followed by Chunleimycin, ethylallicin and matrine, with the EC50 of 26.35 mg/L, 
28.65 mg/L and 339.13 mg/L, respectively. Polyxins showed the lowest effect with an EC50 of 1 150.29 mg/L. Microbiological 
fungicide of Bacillus subtilis showed better effect than Trichoderma harzianum, with an EC50 of 3.2×106
 CFU/g. The four 
screened fungicides of Jinggangmycin, Chunleimycin, ethylallicin and B. subtilis showed antifungal effects on different strains of 
fusarium pathogen. The in vivo inoculation test and field test showed that Jinggangmycin and B. subtilis had remarkable control 
effects on the pathogen causing stem rot disease in passion fruits, with in vivo inoculation control efficacy of 58.8% and 70.6%, 
respectively, and field control efficacy of 53.7% and 61.3%, respectively.【Conclusion】Two biological fungicides of B. subtilis
and Jinggangmycin were screened out for their optimal control effects on the stem rot disease in passion fruits. It can provide 
alternatives or effective supplements for chemical fungicides for the control of stem rot disease, which could be promoted and 
applied.]]></description>
<pubDate>2022/11/29 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[KUANG Ruibin , YANG Min, ZHOU Chenping, YANG Hu, HUANG Bingxiong, WEI Yuerong]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>KUANG Ruibin , YANG Min, ZHOU Chenping, YANG Hu, HUANG Bingxiong, WEI Yuerong</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202210012&flag=1]]></guid><cfi:id>11</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Construction and Identification of Infectious Clone from Sri Lankan Cassava Mosaic Virus Tvm3 Isolate]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202308014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Sri Lankan cassava mosaic virus (SLCMV) is a circular single-stranded DNA virus, containing two components of DNA-A and DNA-B. SLCMV is a member of the genus Begomovirus in the family Geminiviridae, which is a persistent threat to the world’s cassava industry. In 2018, the SLCMV was found and reported for the first time in cassava from Fujian and Hainan provinces, China. However, the viral resistance of cassava varieties in domestic was unknown and the germplasm resources for disease resistance was lacking. To further explore the genetic background of domestic cassava germplasm resources resistance to mosaic disease and the mechanism of interaction between virus and host, SLCMV infectious clone was constructed and verified.【Method】In this study, SLCMV TVM3 strain was used as the object, and its 1.10 mer DNA-A and 1.08 mer DNA-B components were respectively constructed into pCAMBIA1301 vector, and the viral infectious clone vectors pCAMBIA1301-DNA-A (pDNA-A) and pCAMBIA1301-DNA-B (pDNA-B) were obtained. Agrobacterium tumefacien GV3101 mediated pDNA-A and pDNA-B co-infected tobacco and Arabidopsis plants. 【Result】At 14 d post inoculation, the systematic leaves of tobacco plants produced severe mosaic and distortion symptoms, while the systematic leaves of Arabidopsis plants showed mild distortion at 18 d post inoculation. Total DNA was extracted from SLCMV infected tobacco and Arabidopsis plants leaves, and viral DNA-A and DNA-B components were detected by PCR method. In addition, the virus infection rate of tobacco plants was 100%, while the virus infection rate of Arabidopsis plants was only 40%, and the difference between the two group was significant (P < 0.05).【Conclusion】The SLCMV infectious clone have been successfully constructed, which could infect tobacco and Arabidopsis plants, and the viral infection rate reached 96.67% in tobacco plants. Furthmore, the DNA sequences of infectious clone in pDNA-A and pDNA-B are 1.10 mer and 1.08 mer length of the original virus genomic DNA-A and DNA-B components, respectively, whichdid not contain repetitive coding region sequences. This study provides an important basis for the further explore the genetic background of domestic cassava germplasm resources resistance to mosaic disease and the pathogenesis of virus .]]></description>
<pubDate>2023/10/20 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[YU Naitong1,2, XIAN Shuli1, YIN Huixiang1, ZHAO Yuhan3, ZHENG Xiaobao1, LIU Zhixin1,2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>YU Naitong1,2, XIAN Shuli1, YIN Huixiang1, ZHAO Yuhan3, ZHENG Xiaobao1, LIU Zhixin1,2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202308014&flag=1]]></guid><cfi:id>10</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Identification of Pathogen Causing Root Rot in Polygonatum cyrtonema Hua and Screening of Biocontrol Bacterias]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202308015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The objective of this study was to identify the pathogen causing for root rot in Polygonatum cyrtonema Hua in Guizhou Province and to screen for biocontrol bacterias capable of effectively antagonizing this pathogen. Ultimately, this research aims to establish a foundation for environmentally friendly control strategies against P. cyrtonema Hua root rot.【Method】 Diseased tubers of P. cyrtonema Hua were collected from Taijiang County, Guizhou Province. These samples underwent a sreices of procedures including isolation, purification, pathogenicity verification and identification. Both morphological and molecular biology techniques were employed for the identification process. Strains exhibiting significate inhibitory activity were singled out through dual-culture tests. Based on the analysis of culture shape, morphological traits and phylogenetic tree, its taxonomic status was clarified, and the inhibitory effect of its fermentation broth on the mycelial growth of pathogenic bacterias was determined. 【Result】 Out of 28 fungi isolated from the diseased samples, 12 were found to induce evident symptoms of root rot. Among these, strain 2GF1 exhibited the most severe disease symptoms. Both morphological traits and phylogenetic tree analysis confirmed that the all 12 pathogens belonged to Fusarium oxysporum.In the dual-culture tests, the biocontrol strain WHD01 demonstrated the most potent antagonistic effect with an inhibition rate of 95.21%. Additionally, the bacterial strain WHD04 also displayed a significant antagonistic effect with an inhibition rate of 78.99%. Based on morphological and molecular identification, the strains WHD01, WHD02, WHD03, and WHD04 were identified as Trichoderma virens, Phlebiopsis gigantea, Bacillus amyloliquefaciens, and Bacillus velezensis, respectively. The inhibitory effects on the growth of pathogenic mycelia at a fermentation broth concentration of 200 mL/L were as follows: 32.70% (Trichoderma viridans), 26.72% (Phlebiopsis gigantea), 33.05% (Bacillus amyloliquefaciens) and 33.52% (Bacillus Velez), respectively. 【Conclusion】This study successfully identified F. oxysporum as the causative pathogen of root rot in P. cyrtonema Hua in Taijiang County, Guizhou Province. Moreover, four biocontrol bacterias with effective antagonistic effects against this pathogen were identified. These findings lay a the groundwork for the development of eco-friendly strategies for managing of root rot in P. cyrtonema Hua.]]></description>
<pubDate>2023/10/20 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[WANG Feng1 ，WEI Guoyu2 ，ZHAO Wei2 ，WANG Hualei2 ，LI Zhu3 ，QIU Hongbo2 ，HU Anlong2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>WANG Feng1 ，WEI Guoyu2 ，ZHAO Wei2 ，WANG Hualei2 ，LI Zhu3 ，QIU Hongbo2 ，HU Anlong2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202308015&flag=1]]></guid><cfi:id>9</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effects of Combined Application of Microbial Fertilizer and Chemical Fertilizer on Production of Chinese Chive and Occurrence Amount of Bradysia odoriphaga]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202306011&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study aimed to clarify the value of microbial fertilizer in the production of Chinese chive, 
and screen the best scheme for combined application of microbial fertilizer with chemical fertilizer in the high quality and 
safe production of Chinese chive and control of Bradysia odoriphaga. 【Method】Xuejiu 791 was taken as the research 
object, 8 treatments of microbial fertilizer combined with chemical fertilizer were set up, namely, the recommended amount of 
chemical fertilizer combined with 30%, 50%, 70%, 100% of the recommended amount of microbial fertilizer (T2~T5), and the 
recommended amount of microbial fertilizer combined with 70%, 50%, 30%, and 0% of the recommended amount of chemical 
fertilizer (T6~T9). The effects of different fertilization schemes on the yield and growth traits of Chinese chives was estimated by 
indoor single factor experiment to screen the better fertilization schemes; and the effects of above better fertilization schemes on 
the yield and quality of Chinese chives and the occurrence of B. odoriphaga were verified by field experiments.【Result】The 
indoor experiment showed that the growth traits of Chinese chives were significantly improved by microbial fertilizer, with plant 
height, stem diameter, maximum leaf width, plant weight per plant and number of fibrous root significantly increased. Among all 
the fertilization schemes, the best growth traits of Chinese chives were found in T5, T6, and T7 treatment, especially for plant 
height, which was 27.87, 27.59 and 28.84 cm, respectively, and significantly differed from that in other treatments. The weight 
per plant was 0.99, 0.93 and 0.94 g, respectively, which was significantly different from that in other treatments except T4 and 
T9. The growth traits of Chinese chives did not differ among T5, T6 and T7. Based on the above results and the economic point 
of view, T6 and T7 were judged to be the optimal fertilizer scheme. The result of field experiment showed that the application 
of T6 scheme of indoor experiment (FT6 treatment) significantly increased the plant weight, stem diameter, pseudostem length, 
plant height, maximum leaf width and number of fibrous root of Chinese chives in greenhouse by 29.41%, 1.70%, 12.19%, 
7.58%, 17.23% and 40.60%, respectively, compared to the control (FT1). The application of T7 scheme of indoor experiment 
in field (FT7 treatment) significantly increased the weight per plant, stem diameter, pseudostem length, maximum leaf width 
and number of fibrous root of Chinese chives by 23.22%, 4.21%, 11.94%, 9.47% and 16.99%, respectively, compared to the 
control (FT1), but the increase amplitude of each growth character was less than that in FT6. The yield of Chinese chives in FT6 
and FT7 treatment was significantly increased by 48.39% and 12.99%, respectively compared to FT1. The quality of Chinese 
chives in FT6 and FT7 treatment was significantly improved, soluble sugar content and soluble protein content were increased 
by 32.72%,10.45% and 6.76%, 6.60%, respectively, compared to FT1. The growth traits of Chinese chives in FT6 were better 
than those in FT7. FT6 and FT7 treatment inhibited the occurrence of B. odoriphaga, and the inhibition rates were 50.15% and 
22.16%, respectively, compared to the control. 【Conclusion】Compared with the single application of chemical fertilizer, the 
combined application of microbial fertilizer and chemical fertilizer effectively improve the yield and quality of Chinese chive, 
and inhibit the occurrence of B. odoriphaga. The best recommended scheme was 70% of the recommended amount of fertilizer 
combined with 100% of the recommended amount of microbial fertilizer.]]></description>
<pubDate>2023/8/17 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[LIU Jiawei1
，ZHENG Changying1
, CHI Xiuli2
, JIANG Yajun3
, SUN Lijuan1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>LIU Jiawei1
，ZHENG Changying1
, CHI Xiuli2
, JIANG Yajun3
, SUN Lijuan1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202306011&flag=1]]></guid><cfi:id>8</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effects of N,N-Dimethylformamide on Infertility and Palatability of Quinestrol]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202306012&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was carried out to clear and definite that the addition of efficient and safe auxiliary 
N,N-Dimethylformamide (DMF) can improve the sterility of quinestrol baits, and improving the palatability of quinestrol 
baits to mice.【Method】The mice were divided into three groups C, T1 and T2 fed with blank bait, quinestrol bait and 
DMF-quinestrol bait, respectively, and the bait consumption was calculated. The male and female mice of three treatment 
groups were caged for 7 days, and the pregnancy and delivery of the female mice were recorded. The high-performance 
liquid chromatography used to detection the content of quinestrol in brown rice in the different formulations of baits. 【Result】
Both the T1 and T2 groups had significant effects on the body weight of male and female mice. The wet weights of the kidney 
organs in the T1 and T2 groups were 16.4% and 19.7% lower than the C group, respectively. Quinestrol had significant 
inhibitory effect on the reproduction of female mice, with the reproductive rate of female mice in T1 and T2 groups decreased to 37.5% and 25.0% of group C, and the number of litters was only 35.0% and 11.1% of group C, respectively. DMF did not 
significantly improve the palatability of quinestrol drug bait, but the results of high-performance liquid chromatography showed 
that the content of quinestrol in brown rice in the T2 group was 1.5 times higher than that in the T1 group. DMF increased the 
permeability of quinestrol in brown rice, thereby increasing the drug intake of mice.【Conclusion】This study shows that DMF 
can effectively dissolve quinestrol and enhance its permeability in brown rice, improving the contraceptive effect of quinestrol 
drug bait,and has the potential to be developed into a promising contraceptive formulation.]]></description>
<pubDate>2023/8/17 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[QIN Jiao1
，JIANG Hongxue1
，LIU Quansheng2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>QIN Jiao1
，JIANG Hongxue1
，LIU Quansheng2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202306012&flag=1]]></guid><cfi:id>7</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Identification and Virulence Assay of the Entomogenous Fungus FE-1 Strain Isolated from Phenacoccus solenoposis]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202306013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】This study aimed to evaluate the biocontrol potential of an entomogenous fungus strain 
isolated from Phenacoccus solenoposis, for providing the basis of its development as a biocontrol agent.【Method】
The strain was isolated and purified from the diseased P.solenoposis using tissue isolation method. Based on the cultural 
traits, morphological traits and phylogenetic tree analysis, its taxonomic ststus was clarified. The infection mode of strain 
on P.solenoposis was explored by scanning electron microscope，which clarified its ability to infect P.solenoposis. The 
pathogenicity of the strain to P.solenoposis was measured by leaf dipping and insect dipping method. 【Result】A strain 
FE-1 was isolated and purified from the diseased P.solenoposis, the morphological traits results showed that the colony 
diameter of strain FE-1 ranged from 50 to 60 mm at 5 days on PDA medium , and the colony was orange-white in front. The conidia morphology had two types, which small type was long column shape and large spore was sickle shape. The strain FE-1 
was identified as Fusarium equiseti based on cultural traits, morphological traits and phglogenetic tree analysis. The strain 
FE-1 infected into the insect body cavity of P.solenoposis mainly by conidia germination forming germ tubes at the epidermis 
for growing horizontally, and the formation of expanded appressorium at the specific contact epidermis, which clarified its ability 
to infect P.solenoposis. The results of indoor toxicity measuremerts showed that the pathogenicity of strain FE-1 against female 
adults of P.solenoposis was gradually increased with the concentration of conidial suspension. At the spore concentration of 
1×108
 CFU / mL, LT50 was 3.32 days, while at 7 days after inoculation, the adjusted mortality of P.solenoposis female adults 
was 87.50±1.79%, LC50 was 1.8×105
 CFU /mL. 【Conclusion】A strain FE-1 isolated and purified from the diseased 
P.solenoposis can form expanded appressorium at the specific contact epidermis for infection, which clarifies its ability to infect 
P.solenoposis. Meanwhile, the strain FE-1 is highly pathogenic against P.solenoposis, which has great potential for biocontrol 
and provide original material for the biocontrol research of P.solenoposis.]]></description>
<pubDate>2023/8/17 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[BI Keke, DENG Jiaru, ZHANG Jin’ai, SUN Longhua]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>BI Keke, DENG Jiaru, ZHANG Jin’ai, SUN Longhua</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202306013&flag=1]]></guid><cfi:id>6</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Genome-wide Identification and Bioinformatics Analysis of Scavenger Receptor Gene Family in Plutella xylostella]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202305011&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The resistance of Plutella xylostella to chemical insecticides is increasing year by year, which makes RNAi ( RNA interference ) pesticides widely concerned. The P. xylostella SR have phagocytic and pathogenclearing abilities in cellular immunity, rendering them a promising target for RNAi pesticides. This study explores alternative targets for such pesticides by investigating the SR gene family in P. xylostella.【Method】Based on the P. xylostella genome, we comprehensively analyzed the physicochemical properties, transmembrane structure, phylogenetic evolution, secondary and tertiary structure prediction, conserved protein motifs and phosphorylation site prediction of SR gene family. 【Result】The SR gene family of P. xylostella has 12 CD36 protein sequences with similar physicochemical properties and functional structures, all of which contain multiple hydrophilic and hydrophobic regions as well as two transmembrane helical regions. Their secondary and tertiary structures feature irregular coiling with the highest serine content in the phosphorylation site. A total of six species, including P. xylostella, Bombyx mori, Anopheles gambiae, Drosophila melanogaster, Manduca 
sexta and Tribolium castaneum contained 117 CD36 protein sequences that were classified into five distinct branches on the evolutionary tree. Genes with similar structures were grouped together within the same branch, indicating a high conservation throughout evolution. CD36px4 and CD36px5 exhibit more intricate tertiary structures. The motif 19 and motif 20 are exclusive to CD36px4, suggesting their potential involvement in species evolution. With a staggering 143 phosphorylation sites, CD36px4 may play a crucial role in the phagocytosis and clearance of apoptotic cells.【Conclusion】The 12 SRs identified are transmembrane proteins with abundant phosphorylation sites, predicted to participate in various physiological processes such as intracellular and extracellular material transport, signal transduction, energy metabolism, and apoptosis regulation. They also possess the functions of mediating phagocytosis and clearance of cellular debris. These receptors represent a promising target for 
RNAi-based pesticides. The results have provided a crucial foundation for the screening of RNAi pesticide targets.]]></description>
<pubDate>2023/7/5 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[TAN Jianbin, XU Jin, SHI Peiqiong, ZHOU Hongkai]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>TAN Jianbin, XU Jin, SHI Peiqiong, ZHOU Hongkai</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202305011&flag=1]]></guid><cfi:id>5</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Study on the Bio-Control Efficiency of Peanut Pod Rot by Bacillus ZKY01]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202303011&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Pod rot caused by Fusarium spp. is a major soil-borne peanut disease in Guangdong, which affects the yield and quality of peanut seriously. In order to provide a theoretical basis for green prevention and control of pod rot, a biocontrol bacterium was screened and its identification, physiological biochemistry characteristics and control efficiency in fields were evaluated.【Method】Strains with wide amitbacterial spectrum activity the pathogens of peanut pod rot were screened from biocontrol Bacillus strains preserved in laboratory by plate antagoinsm method, and identified by sequence amplification and phylogenetic analysis of 16S rDNA, gyrA, and gyrB sequences. Then, their physiological and biochemical characteristics, carbon source utilization capacity of Biolog-GN plate, biofilm formation in MSgg, BGM1, and BGDM media were determined. Meanwhile, the control effects of these strains on peanut pod rot were conducted by field plot tests.【Result】
A Bacillus strain ZKY01 with wide antibacterial spectrum activity was selected, and its plate inhibition rates on Fusarium sp. G15, F. oxysporum R5 and F. solani R4.2 were 72.23 (±3.36)%, 54.14 (±10.33)% and 69.37 (±2.17)%, respectively. According to the phylogenetic analysis, the strain ZKY01 was identified as Bacillus velezensis. ZKY01 could grow normally in 3% to 10% NaCl solution, and temperature suitable for its growth ranged from 10 ℃ to 50 ℃. It could use 50 kinds of carbon sources and form robust pellicles on MSgg, BGM1 and BGDM liquid media. Field control test results showed that ZKY01 wettable powder drenching at seedling stage had a control effect of 95.14% against Fusarium-caused peanut pod rot.【Conclusion】Bacillus velezensis ZKY01 has great application potential in the biological control of peanut pod rot caused by Fusarium spp.]]></description>
<pubDate>2023/5/5 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[WEI Xinying1, XING Juejun1, MIAO Shuang1, QIN Di1, DENG Xiaoyin2, LI Huayong3, YU Guohui1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>WEI Xinying1, XING Juejun1, MIAO Shuang1, QIN Di1, DENG Xiaoyin2, LI Huayong3, YU Guohui1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202303011&flag=1]]></guid><cfi:id>4</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Study on the Antimicrobial Mechanism of Benzothiazolinone Against Kiwifruit Ulcerative Pathogens]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202303012&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】By measuring a series of index changes of Pseudomonas syringae pv. Actinidiae (Psa) after treatment with benzothiazolinone aqueous emulsion, the antibacterial mechanism of benzothiazolinone aqueous emulsion against Psa was revealed, which would provide a theoretical basis for the control of kiwifruit ulcer disease with benzothiazolinone.【Method】Psa was treated with benzothiazolinone diluted 10 times (X10), benzothiazolinone stock solution (Y) and sterile water (CK). The enzymatic activities of catalase (CAT) and superoxide dismutase (SOD) were determined 
by using kits and the soluble protein content was determined by Coomassie Brilliant Blue staining. Changes in the cell membrane structure of Psa were observed by fluorescence microscopy and scanning electron microscopy and cell cycle assays were performed by flow cytometry.【Result】The study showed that Psa growth and reproduction were significantly inhibited by benzothiazolinone treatment, which shortened the logarithmic growth period of Psa, caused cell membrane rupture, cell contents leakage and increased cell death, and the final amount of mycobacteria was only 5.5% of the control. Psa had oxidative stress response to benzothiazolinone, and the enzyme activities of Psa were significantly increased after treatment, showing a trend of increasing first and then decreasing. After treatment of benzothiazolinone, CAT enzyme activity reached a peak of 118.795 U/mg at 10 h and SOD enzyme activity reached a peak of 1 060.452 U/mg at 4 h. Bacterial protein content of X10 and Y differed significantly at 12 h compared with the control, decreasing by 47.1% and 73.4%, respectively. With the increase of the concentration of benzothiazolinone, more G0G1 phase cells piled up and the percentage of cells increased from 30.27% in the control group to 45.23% in treatment group Y. The percentage of S phase cells decreased from 58.84% in the control group to 41.86% in treatment group Y. The G0G1 phase cells in treatment group Y were 56.8% higher than those in CK and 23.7% lower than those in CK in S phase.【Conclusion】Benzothiazolinone ruptures bacterial cell membranes, causes oxidative stress response in Psa, and inhibits Psa growth by inhibiting DNA synthesis in bacterial cells. It can effectively prevent and control bacterial ulcer disease of kiwifruit.]]></description>
<pubDate>2023/5/5 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[HE Zimei1, YIN Maoling1, ZHANG Yu1,2, LUO Xuegang1,2, WANG Zhengqian3, LI Chunhua4]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>HE Zimei1, YIN Maoling1, ZHANG Yu1,2, LUO Xuegang1,2, WANG Zhengqian3, LI Chunhua4</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202303012&flag=1]]></guid><cfi:id>3</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Control Effects of Different Agents Against Blossom Blight of Kiwifruit]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202303013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The research was carried out to clarify the field control effects of six agents on blossom blight of kiwifruit, and to screen efficient and safe agents for guiding the field production of kiwifruit.【Method】The field control experiment was carried out by using random block design, and the incidence rate and disease index of blossom blight of kiwifruit after the application of six agents were determined, and the control effects were evaluated by calculating the change values of incidence rate and disease index before and after treatment. The safety of each agent was evaluated by its effects on the growth situation of kiwifruit plants and the development characteristics of flowers and fruits.【Result】Six agents showed different control effects on blossom blight of kiwifruit, and all of them were safe to kiwifruit plants. The results of field trials showed that there was no significant difference in incidence rate and disease index between the treatment groups and the CK group before control. After two consecutive treatments, the incidence rates of treatment groups and CK group showed an increasing trend compared with those before control, and the change values of the disease situation were significantly different, among which the change value of the CK group was the largest, and the incidence rate and disease index increased by 13.29% and 6.84, respectively. The change values of kasumin • brothermothingonil and tetramycin•propyrithiazole were the smallest, with the increase of incidence rate less than 1.50% and the increase of disease index less than 1.00. The field control effect corresponded to the disease situation. After two consecutive treatments, the treatment of kasumin • brothermothingonil showed the highest control effect, followed by tetramycin•propyrithiazole, both of which were above75%. All agents had no phytotoxicity symptoms and other adverse effects on kiwifruit plants after the application of agents, and showed different degrees of flower protection, fruit protection and yield increase compared with the CK group. The results showed that among the six agents, kasumin • brothermothingonil and tetramycin • propyrithiazole were the most effective in delaying the transmission and spread of the pathogen of blossom blight of kiwifruit, reducing the disease severity, and controlling blossom blight of kiwifruit efficiently and safely.【Conclusion】Two effective and safe agents - kasumin • brothermothingonil and tetramycin • propyrithiazole are screened for blossom blight of kiwifruit, which can be promoted in field production of kiwifruit.]]></description>
<pubDate>2023/5/5 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[CHEN Jia1, SHI Jinqiao1, RAN Fei1, CHEN Tingting1, LONG Youhua1,2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>CHEN Jia1, SHI Jinqiao1, RAN Fei1, CHEN Tingting1, LONG Youhua1,2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202303013&flag=1]]></guid><cfi:id>2</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Influence of Subculture of Beauveria bassiana on Virulence and Spore Production of Spodoptera litura Larvae and its Strain Rejuvenation]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202402009&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study aimed to investigate the impact of continuous subculture of Beauveria bassiana on the virulence and spore production of second-instar larvae of Spodoptera litura. Additionally, a study on strain rejuvenation was conducted to offer references for the biological control of B. bassiana against S. litura.【Method】The primary strain, designated as F0 (SCAUJH19), was inoculated on PDA medium, and subsequent generations (F1~F10) were obtained through ten consecutive subcultures. The second-instar larvae of S. litura were exposed to 1×108 CFU/mL B. bassiana treatment by insect dipping method. The virulence of different sub-cultured strains against second-instar larvae was determined, and spore production for each generation was quantified. A model was constructed to analyze the relationship between virulence, spore production and successive subcultures in B. bassiana strains and laws of virulence degeneration. Live insect rejuvenation method was implemented on strains from generations F4, F6, F8 and F10, followed by a comprehensive comparison and analysis of the virulence effects on second-instar larvae before and after rejuvenation, along with measuring their spore productions.【Result】No significant difference in virulence was observed among F1~F5 subcultures of B. bassiana strains, with mortality rates ranging from 42% to 45% when treat for 7 d. However, the mortality rate of F6, F8 and F10 strains against S. litura decreased to 37%, 22.94% and 12.17%. Spore production analysis indicated that F1~F5 strains maintained a spore quantity of above 9.65~10.43×108 CFU/mL, whereas spore quantities of F6, F8 and F10-generation strains decreased to 7.62×108 CFU/mL, 5.14×108 CFU/mL and 4.69×108 CFU/mL. The constructed model revealed an exponential decrease in spore production with subcultures from the F5 generation, with a positive correlation between spore production and virulence. Strains with fewer subculture generations exhibited higher spore production and greater pathogenicity to second-instar larvae of S. litura. After the rejuvenation of F4, F6, F8 and F10 strains with live insect rejuvenation method, the corrected mortality of the treated strains of S. litura second-instar larvae remained above 50%, surpassing the highest mortality of the original strains, and the spore production of the rejuvenated strains exceeded that of the pre-rejuvenated strains.【Conclusion】After 4 to 5 generations of subculture, B. bassiana demonstrates a gradual decrease in spore production and virulence against second-instar larvae of S. litura and strain rejuvenation is needed. The sub-generation of B. bassiana could be effectively restored or improved to its original characteristics through the live insect rejuvenation method. Spore production of the same strain can be served as a valuable reference index for evaluating its virulence.]]></description>
<pubDate>2024/3/21 0:00:00</pubDate>
<category><![CDATA[Plant Protection]]></category>
<author><![CDATA[CHEN Jijuan, ZHU Bifeng, LI Haolong, SHAUKAT Ali, WU Jianhui]]></author>
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<atom:name>CHEN Jijuan, ZHU Bifeng, LI Haolong, SHAUKAT Ali, WU Jianhui</atom:name>
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