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<title cf:type="text"><![CDATA[Editorial Department of Guangdong Agricultural Sciences -->Animal Husbandry & Veterinary · Aquaculture]]></title>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Research Progress on Selection Methods of Abdominal Fat of High Quality Chicken]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201909017&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[High-quality chickens are usually raised for a long time, and hens are always raised to near sexual maturity 
with a lot of fat doposition. Chicken body fat mainly includes abdominal fat depostion, subcutaneous fat and intermuscular 
fat. Modern commercial broilers contain 150 to 200 grams of fat per kilogram of body weight, and a vast majority of fat is not 
physiologically necessary, which not only leads to waste of feed, but also directly affects the processing of meat products, 
and reduces the slaughter rate and economic benefits. Excessive deposition of fat has become a serious problem in broiler 
production, and excessive abdominal fat has been an important factor that restricts the production efficiency of broilers. 
Therefore, it is of great significance to reduce abdominal fat deposition when selecting and breeding the chicken. Scholars 
at home and abroad have made some progress in the selection of abdominal fat from the aspects of genetics and breeding, 
physiology and biochemistry and so on. In this paper, the selection methods of abdominal fat deposition in breeding broilers 
were reviewed from two aspects: direct selection (direct selection by sibling slaughtering) and indirect selection. The indirect 
selection method mainly includes the selection of feed conversion rate, the selection of body size traits, the selection of blood 
biochemical indexes and genetic selection.]]></description>
<pubDate>2019/11/15 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[郎倩倩，肖　昊，王　丽，高开国]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>郎倩倩，肖　昊，王　丽，高开国</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201909017&flag=1]]></guid><cfi:id>146</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effects of Recombinant Human Lactoferrin on Growth Performance and Serum Biochemical Index of Weaned Piglets]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201909018&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The experiment was conducted to investigate the effects of recombinant human lactoferrin (rHL) 
on the growth performance and serum biochemical index of weaned piglets.【Method】One hundred and eighty 28-dayold weaned piglets (with uniform weight), were assigned randomly to 6 treatment groups and there were 3 replicates in each 
group with 10 piglets per replicate (5 males and 5 females). The control group A were fed with a basal diet, and group B piglets were fed a basal diet supplemented with antibiotic (1 g/kg 10% bacitracin zinc + 200 mg/kg 10% colistin). Groups C, D, 
E and F were fed with basal diets supplemented with 160, 320, 480 and 640 mg/kg rHL, respectively. The experiment lasted 
for 28 days.【Result】The results showed as follows: (1) the final weight and average daily gain(ADG) of groups B, C, D, E and 
F were higher than those of group A (P ＜ 0.05). The daily intake of groups B and E was significantly higher than that of group 
A(P ＜ 0.05). The ratio of weight gain to intake of groups B, D, E and F was significantly lower than that of groups A(P ＜ 0.05). 
The diarrhea index of groups B, D, E and F was lower than that of groups A and C(P ＜ 0.05). (2) The concentrations of serum 
albumin and TP of group E were higher than that those of group A(P ＜ 0.05). The urea nitrogen of groups B and E was higher 
than that of group A(P ＜ 0.05). (3)The T-AOC level of groups C, D, E and F was higher than that of group A(P ＜ 0.05). The 
GSH-Px activity of groups D and F was higher than that of group A(P ＜ 0.05). The T-SOD level of group E was higher than that 
of groups A and B(P＜ 0.05). The MDA content of group A was lower than that of groups C, D, E and F(P＜ 0.05).【Conclusion】
The above data showed the supplemented rHL could improve the growth performance, serum biochemical index and antioxidant 
ability of weaned piglets. The optimum level of rHL was 480 mg/kg based on the test results.]]></description>
<pubDate>2019/11/15 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[梁耀文 1
，舒会友 2，黄新全 3， 陈 玲 4，刘 燊 1
，张辉华 1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>梁耀文 1
，舒会友 2，黄新全 3， 陈 玲 4，刘 燊 1
，张辉华 1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201909018&flag=1]]></guid><cfi:id>145</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Study on the Effect of Nitric Oxide Treatment on the Inhibition of Browning of Fresh-cut Pueraria at Low Temperature]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201909020&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Fresh-cut Pueraria (southern medicinal plant) is prone to browning, the effect of nitric oxide 
(NO) treatment on the inhibition of browning of fresh-cut Pueraria at was studied in this paper.【Method】Fresh-cut 
Puerariawere dipped into 0.5, 1.0 and 2.0 mmol/L sodium nitroprusside (SNP) solution respectively for 15 min and then 
were drained and sealed with polyethylene and stored at constant temperature . And the physiological and biochemical 
indexes related to browning were determined regularly.【Result】The results showed that, compared with the control group, 
the browning of fresh-cut Pueraria was inhibited by being treated with different concentrations of sodium nitroprusside.The 
browning index of treatment with 2.0 mmol/L sodium nitroprusside was lowest, which was only 62.4% of the control at 10 d. 
Based on the determination of the physiological and and biochemical indexes related to browning, different concentrations of 
SNP treatment could inhibit the activity of PAL after 6 d, decrease the total phenol content, and activity of PPO and promote the content of total flavonoid during the whole storage, and the above indexes were inhibited or promoted significantly with 
the increase of the concentration of SNP. The contents of total phenol and flavonoid of the treatment with 2.0 mmol/L SNP 
were 66.4% and 542% of the control at 10d, while the activity of POD and the content of malondialdehyde were decreased 
significantly by the treatment of 2.0 mmol/L SNP during the whole storage, which could inhibit the brownin go ffresh-cut 
Pueraria. According the correlation analysis on the physiological indexes of fresh-cut Pueraria treated with 2.0 mmol/L SNP, 
the browning degree was positively correlated with PAL activity, total phenol contentand flavonoid content and negatively 
correlated with the activity of POD and PPO, however, it was not correlated with MDA significantly.【Conclusion】2 mmol/L 
SNP treatment could effectively inhibit the browning of fresh-cut Pueraria and improve its functional quality, providing reference 
to the color protecting of fresh-cut Pueraria.]]></description>
<pubDate>2019/11/15 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[宋康华 1
，张鲁斌 1,2，冯显爵 2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>宋康华 1
，张鲁斌 1,2，冯显爵 2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201909020&flag=1]]></guid><cfi:id>144</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Improvement of Nonylpheol-induced Embryonic Development Toxicity in Carassius auratus cuvieri by Tea Polyphenols]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201909019&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Nonylphenol (NP) is an environmental estrogen pollutant. The study was to explore the toxic 
effect of NP on the embryonic development of Carassius auratus cuvieri, and to investigate the improvement of the NPinduced toxicity by tea polyhenols.【Method】The tail bud embryos were exposed to different concentrations of NP, and the 
toxic effects of NP on the embryonic development of C. auratus cuvieri were analyzed by comparing the changes of hatching 
rate of embryos and the abnormal rate of larval under toxic treatment, with the clear water as control group. Subsequently, the 
embryos were soaked in NP liquid added with tea polyphenols, and the changes of the hatching rate and the abnormal rate 
were compared between NP control group and tea polyphenols experimental group in order to judge the improvement effect 
of tea polyphenols.【Result】Compared with the ethanol control group, the low and the medium concentration of NP 
(0.04-0.40 mg/L) not only reduced the hatching rate of embryos significantly but also increased the abnormal rate of larvae 
significantly, and the abnormal rate increased with the increasing of NP concentration. The high concentration of NP (4.00 
mg/L) had strong toxicity to embryonic development, and the embryos with this concentration stopped to develop within 24h of 
exposure to NP. After adding the tea polyphenols (0.35-35.00 μg/L) into NP, the tea polyphenols (3.50 μg/L) could significantly 
improve the hatching rate of embryos. There was no significant difference in hatching rate between other experimental group and NP control group. In addition, 0.35-35.00 μg/L tea polyphenols could significantly reduce the abnormal rate of larval 
induced by NP.【Conclusion】NP has certain toxicity to the embryos of C. auratus cuvieri, and adding appropriate amount of 
tea polyphenols can improvement the embryonic toxicity induced by NP.]]></description>
<pubDate>2019/11/15 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[陈建华，谢艳颖]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>陈建华，谢艳颖</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201909019&flag=1]]></guid><cfi:id>143</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Analysis on Canonical Correlation Between Egg Productionand Comb Traits of 72-week-old Chicken]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201908016&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】 The study was to explore the correlation between comb traits and egg production traits of
72-week-old chickens【Method】 The comb traits and egg production traits of 72-week-old chickens were determined
by using the resource population F2 established by Dongxiang Green-shell laying hens and single-comb White Leghorn
chickens as parents. 【Result】 The results showed that 24 pairs of correlations among 9 variables reached extremely
significant. Among the comb traits, the correlation between comb weight and comb area was the largest with the correlation
coefficient of 0.846 (P ＜ 0.01). As for the egg production traits of 72-week old, the correlation between egg number and egg
weight was the greatest with the correlation coefficient of 0.139(P ＜ 0.01). Among the traits of two groups, the correlation
between comb length and egg weight of 72-week old was the greatest with the correlation coefficient of 0.118 (P ＜ 0.01).
The first canonical correlation coefficient between comb traits and production traits was 0.203, which was extremely
significant. 【Conclusion】 The correlation between the two traits was mainly affected by comb height, comb area and egg
number, egg weight and eggshell strength of 72-week old.]]></description>
<pubDate>2019/9/24 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[马　猛，王克华，曲　亮，窦套存，沈曼曼，王星果，郭　军]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>马　猛，王克华，曲　亮，窦套存，沈曼曼，王星果，郭　军</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201908016&flag=1]]></guid><cfi:id>142</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Establishment and Application of Duplex Quantitative Real-TimePCR Method for Detection of Equine Rhinopneumonitis]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201908017&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】 The study was to establish of a duplex quantitative real-time PCR method for rapid and
accurate detection of equine herpesvirus 1 (EHV1) and equine herpesvirus 4 (EHV4). 【Method】 Based on the complete
genome sequence of EHV1 and EHV4, according to the conserved sequences of glycoprotein B (gB) gene in GenBank, a
series of specific primers and probes were designed. By selecting suitable primers, optimizing reaction conditions, two kinds
of equine herpesviruses (EHV1, EHV4) were detected. 【Result】 The standard strains such as EHV1, EHV4, EIV, EAV,
EVJ and EIAV were detected, among which EHV1 and EHV4 was positive and other stains were negative. The minimum
detection limit was 1.47×102copies/μL. The positive detection rate in 64 clinical samples was 14.06%, which was 100%
consistent with virus isolation. 【Conclusion】 The duplex quantitative real-time PCR detection method can be directly
applied to detect and distinguish EHV1 and EHV4, and the test results of specificity and sensitivity of EHV1/EHV4 can be
obtained in a short time.]]></description>
<pubDate>2019/9/24 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[林志雄 1
，鱼海琼 1
，王　莹 1
，佟铁铸 1
，温肖会 2，张　利 3，翟建新 3]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>林志雄 1
，鱼海琼 1
，王　莹 1
，佟铁铸 1
，温肖会 2，张　利 3，翟建新 3</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201908017&flag=1]]></guid><cfi:id>141</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Comparison of Digestive Enzyme and Antioxidant EnzymeActivities of Amphiprion ocellaris Cultured inNatural Seawater and Artificial Seawater]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201908018&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】 The study was to investigate the changes of digestive enzyme and antioxidant enzyme
activities in juvenile Amphiprion ocellaris cultured in natural seawater and artificial seawater. 【Method】 Natural seawater and artificial seawater culture experiments of A. ocellaris were carried out, and the samples were analyzed after being cultured
for 6, 24, 48 and 96 hours to determine the activities of individual pepsin, intestinal amylase and various tissue antioxidant
enzymes, including superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and glutathione peroxidase (GSH) of
kidney, spleen, liver, gill filament and muscle. 【Result】 The results showed that different culture treatments had significant
effects on pepsin (the activity of pepsin of artificial seawater group was significantly higher than that of natural seawater group),
but had no significant effect on amylase. There were tissue difference in antioxidant enzyme activities of juvenile A. ocellaris
under different culture conditions. In the artificial seawater group, although the activities of antioxidant enzymes fluctuated
with time, the law of the change was not obvious. Pepsin activity of juvenile A. ocellaris in artificial seawater group was lower
than that in natural seawater group only at 6 h and higher than that in natural seawater at other time points, while the amylase
activity showed reverse situation, namely, the pepsin activity was higher than that in natural seawater only at 6 h. The maximum
SOD activity (36.70±3.55 U/mg) was obtained from the sampled muscle after being cultured for 48 h in artificial seawater.
The maximum POD activity of gill filament and muscle presented in natural seawater at 96 h respectively, while that of other
tissues presented in artificial seawater group. The maximum CAT activity of spleen presented in natural seawater at 24 h, and
that of other tissues in the artificial seawater group. The maximum GSH activity of spleen and gill filament presented in natural
seawater group at 96 h, and that of other tissues in artificial seawater group. 【Conclusion】 The results prove that the artificial
seawater culture condition has certain stress on the juvenile A. ocellaris, the individual also has a certain regulation effect on
the stress, and different tissues have different regulation functions.]]></description>
<pubDate>2019/9/24 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[胡　静 1，2，3，周胜杰 1，2，3，杨　蕊 1，2，3，陈　旭 1，2，3，杨其彬 1，2，3，马振华 1，2，3]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>胡　静 1，2，3，周胜杰 1，2，3，杨　蕊 1，2，3，陈　旭 1，2，3，杨其彬 1，2，3，马振华 1，2，3</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201908018&flag=1]]></guid><cfi:id>140</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Optimization of Mixed Enzymatic ModificationProcess of Corncob Dietary Fiber]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201908019&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was to establish a method for enzymatic extraction of corncob dietary fiber and
to optimize the preparation of soluble dietary fiber (SDF) by mixed enzymatic modification of corncob insoluble dietary fibe
(IDF). 【Method】 With the corncob as raw material, the conditions of alkaline protease, α-amylase and saccharification
enzyme pretreatment for extraction of corncob total dietary fiber (TDF) were optimized by single factor experiment. With the
combination of single factor and orthogonal design experiments, the process of SDF preparation from IDF by mixed cellulase
and xylanase modification was optimized. 【Result】 The conditions of bio-enzymatic extraction of corncob TDF were as
follows: solid-liquid ratio of 1∶10, pH 9.0 and 1.4% alkaline protease with enzymatic hydrolysis at 50℃ for 60 min; pH 6.5,
0.3% α-amylase and glucoamylase (1 ∶ 1)with hydrolysis at 60℃ for 60 min, and IDF yield was 69.35%. The optimum  process for the modification of IDF by mixed enzymatic method was as follows: pH 5.0, temperature 50 ℃ , cellulase 1.2%,
xylanase 1.2%, with enzymatic hydrolysis for 6 h and the solid-liquid ratio of 1 ∶ 10. The SDF yield could reach 22.16%.
The water holding capacity, expansibility, and oil binding capacity of the obtained SDF were 6.55 g/g, 6.69 mL/g, and 4.65 g/
g, respectively, which were 40.26%, 48.67% and 74.16% higher than those before modification, respectively. 【Conclusion】
The yield of SDF prepared by mixed enzyme modification from corncob IDF was higher than that of single cellulase or single
xylanase, and the water holding capacity, oil binding capacity, and expansibility of SDF product were significantly improved as
well.]]></description>
<pubDate>2019/9/24 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[刘丽娜 1，2，傅曼琴 2，徐玉娟 2，余元善 2，温　靖 2，吴继军 2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>刘丽娜 1，2，傅曼琴 2，徐玉娟 2，余元善 2，温　靖 2，吴继军 2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201908019&flag=1]]></guid><cfi:id>139</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Current Situation of Slaughtering Industry inGuangdong and Development Countermeasuresin the Post African Swine Fever Era]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201907019&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[The outbreak of African swine fever (ASF) has affected the supervision policy of pig transportation, and
the transformation from“pig transportation”to“meat transportation”has led to a great change in pig slaughtering
industry, and it is imperative for slaughtering enterprises to improve the quality and efficiency of slaughtering and promote
the transformation and upgrading of the enterprises. This paper summarized the current situation of the slaughtering
industry in Guangdong, and analyzed the existing problems of livestock and poultry slaughtering industry from the aspects
of enterprise scale, operation and management, slaughtering supervision, technical requirements and standardization
construction, and expounded the challenges and opportunities faced by the pig slaughtering industry in the post ASF era.
Combined with the epidemic development and consumption characteristics of Guangdong, it is proposed to make use of
the market and the effective guidance of the government, accelerate the standardization construction of pig slaughtering
enterprises of Guangdong, strengthen the management norms of slaughtering industry, enhance the technical research
of slaughter processing, improve the quality of employees, to provide decision-making basis for the transformation and
upgrading of slaughtering industry in Guangdong Province.]]></description>
<pubDate>2019/8/22 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[康桦华，移兰丽，王片片，陈兴勃，彭新宇，向　蓉，梅盈洁，徐志宏]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>康桦华，移兰丽，王片片，陈兴勃，彭新宇，向　蓉，梅盈洁，徐志宏</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201907019&flag=1]]></guid><cfi:id>138</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Rapid Detection Method for Sulfamethazine Residue in Eggs]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201907020&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】 The study was to develope a rapid detection method for veterinary drug residues in
whole egg, albumen, and yolk. 【Method】 Taking sulfamethazine as a case, a method was developed for rapid detection
of sulfamethazine in whole eggs, yolks, and albumens. The sample pretreatment was conducted by using liquid-liquid
extraction to achieve the extraction and purification of target compounds. And the target compounds were detected by high
performance liquid chromatography-electrospray ionization tandem mass spectrometry that with good selectivity and high
sensitivity. 【Result】 As verified by spike matrix test, the method was proved to be stable and reliable. The recoveries of
sulfamethazine in whole eggs, yolks, and albumens were in range of 78.4%-85.2%, 77.9%-89.0% and 83.0% - 88.1%,
respectively. The detection limit of the method was 0.5 ng/g for the target compounds in whole eggs, yolks, and albumens. Finally, the developed method was applied in sulfamethazine analysis of 15 egg samples from Guangzhou City. The results
showed that sulfamethazine was detected in only one sample, and the contents of sulfamethazine in whole eggs, yolks, and
albumens were 0.66, 0.94, and 0.47 ng/g, respectively. 【Conclusion】 Due to no column chromatography procedure in sample
pre-treatment, the method has the advantages of simple operation, low cost and high sensitivity. In addition, this method has
good recovery and stability, which is suitable for rapid detection of sulfadimidine in large quantities of whole egg, albumen, and
yolk samples.]]></description>
<pubDate>2019/8/22 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[黄晓梅 1，2，苏秋权 1
，李亚菲1，2，王威利 1，2，殷秋妙 1，2，伍健光 3，吴维煇 1，2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>黄晓梅 1，2，苏秋权 1
，李亚菲1，2，王威利 1，2，殷秋妙 1，2，伍健光 3，吴维煇 1，2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201907020&flag=1]]></guid><cfi:id>137</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Assessment on Water Quality of Pelteobagrus fulvidracoCulture Ponds with Five Feeding Rates Based onPrincipal Component Analysis]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201907021&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】 The study was to evaluate the dynamic changes of water quality of Pelteobagrus
fulvidraco ponds with different feeding rates and explore the effects of different exogenous feed inputs on the water
quality of P. fulvidraco ponds in order to provide reference for the ecological restoration of large-scale culture ponds of P.
fulvidraco. 【Method】 In P. fulvidraco ponds, the feeding rates of 1.0%, 1.5%, 2.0%, 2.5% and 3.0% were set, and the
main environmental factors in each group were monitored regularly. Combined with Grey Relational Analysis, Principal
Component Analysis (PCA) was used to evaluate the water quality and health status of each pond. Based on the comparative analysis of water quality evaluation results, a feed input model for P. fulvidraco ponds based on good water quality was put
forward. 【Results】 The results showed that CODMn, DO, TN, TP and NH3-N were the main environmental factors affecting
the water quality of P. fulvidraco culture ponds. The pH value had extremely high correlation with other environmental factors,
and the TN had significant correlation with TP and NH3-N. Among five experimental groups, the water quality of group A pond
(feeding rate: 1.0%), group B pond (feeding rate: 1.5%), group C pond (feeding rate: 2.0%), group E pond (feeding rate: 3.0%)
reached water quality grade of class Ⅲ in all stages, which is consistent with the water quality of aquaculture. The water quality
of group D pond (feeding rate: 2.5%) reached water quality grade of class Ⅳ in the middle stage of aquaculture, and the contents
of TN and TP in the water exceeded those of the standard levels, therefore, the water quality pollution was serious. At the
same time, the comprehensive scores of principal components in group A, B and C were 1.2704536, 1.2704664 and 1.270464,
respectively, and the water quality was in good condition. 【Conclusion】 The experimental groups with feeding rates of 1.0%-
2.0% have the best water quality, and excessive feeding rate will reduce the quality and health of aquaculture water.]]></description>
<pubDate>2019/8/22 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[赵晚晨 1
，秦秀东 2，程光平 1
，张伟刚 3，孙玉章 3，李文红 1
，赵学倩 1
，张亚军1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>赵晚晨 1
，秦秀东 2，程光平 1
，张伟刚 3，孙玉章 3，李文红 1
，赵学倩 1
，张亚军1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201907021&flag=1]]></guid><cfi:id>136</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Biological Functions of Plant Extracts and Their Application in Broiler Production]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201906015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[Plant extracts are single or mixed compounds extracted from plants by physical and chemical methods. 
They has the biological functions of growth-promoting, antioxidant, antimicrobial, antiviral and immunity enhancing. As feed 
additives, plant extracts can improve the growth performance, meat quality, antioxidant capacity, immunity and intestinal 
health of broilers. The extraction process, active ingredients and experimental conditions of plant extracts affect the effect of 
plant extracts. This paper summarized the biological functions, influencing factors of plant extracts and their application in 
broiler production, to provide theoretical basis for the wide application of plant extracts in broiler production.]]></description>
<pubDate>2019/8/8 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[李贞明，张贝贝，余　苗，刘志昌，容　庭，马现永，王　刚]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>李贞明，张贝贝，余　苗，刘志昌，容　庭，马现永，王　刚</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201906015&flag=1]]></guid><cfi:id>135</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effects of Overseeding Fast-growing Cold-Season Forage in Winter on Yield and Quality of the New Strains of Hybrid Pennisetum in Next Spring]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201906016&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】 The study was carried out to explore the effects of overseeding fast-growing cold-season 
forage grass in winter on the yield and quality of hybrid Pennisetum in the next spring in Guangdong hybrid Pennisetum
grassland.【Method】 In the row of hybrid Pennisetum that is dormant after winter castration, the fast-growing cold-season 
forage grass Vicia villosa, V. sativa, and Lolium perenne were reseeded in the rows of hybrid pennisetum during the winter 
dormancy after mowing, with non-overseeding group as control, and the yield and nutritional quality of hybrid Pennisetum
were determined during spring cutting in the spring of the next year. 【Result】 The results showed the yield and quality of 
hybrid Pennisetum was improved in the next spring after overseeding. The yield of hybrid pennisetum after the reseeding of 
V. villosa, V. sativa, and L. perenne in November and reseeding of Lolium perenne in December was 11.85, 8.85, 8.83 and 
7.54 t/ hm2(DM), respectively, an increase of 65.5%, 23 .6%, 23.3% and 5.3% over those of the non-overseeding control 
group, respectively. The group with the reseeding of V. villosa has the highest crude protein content of hybrid Pennisetum.【Conclusion】The comprehensive effect of the reseeded V. villosa on the production performance of hybrid Pennisetum was 
the best, followed by the L. perenne and L. perenne reseeded in November. The comprehensive effect of L. perenne was poor, 
which was lower than that of non-overseeding treatment.]]></description>
<pubDate>2019/8/8 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[韩卡文，石秀兰，韦立台，杨　磊，张群珧，韩瑞宏]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>韩卡文，石秀兰，韦立台，杨　磊，张群珧，韩瑞宏</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201906016&flag=1]]></guid><cfi:id>134</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Isolation, Identification and Genetic Evolution Analysis of New Epidemic Strains of Senecavirus A in Guangdong Province]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201906017&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】 The pathogen of blistering disease with nasal and coronet in two swine farms in Guangdong 
province was detected, and new strains of Senecavirus A (SVA) were obtained, which provided materials for subsequent 
research. 【Method】 RT-PCR and real-time RT-PCR were used to screen the clinical samples. The clinical samples 
were isolated, treated and cultured with ST cells and BHK-21 cells. The virus was purified by sucrose density gradient centrifugation method and observed by electron microscopy. The specific primers of SVA were designed to amplify the cell 
supernatant, and sequenced for sequence analysis. The TCID50 of the virus isolated was determined. 【Results】 The swinery 
were diagnosed as SVA by RT-PCR and real-time RT-PCR. It was found that the cultured cells had CPE from the 6th 
generation. The virion observed by electron microscopy was about 25 nm. The amplified sequences were submitted to the NCBI 
Blastn, which were identified as SVA. The TCID50 result of the strain SVA CH-GDBL1-2016 was 106.8 and the TCID50 result of 
the strain SVA CH-GDBL2-2016 was 106.7.【Conclusion】 The strains SVA CH-GDBL1-2016 and SVA CH-GDBL2-2016 
provide a material basis for the subsequent diagnosis and vaccine development of Senecavirus disease.]]></description>
<pubDate>2019/8/8 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[林秀银 1
，温肖会 2，吕殿红 2，翟少伦 2，霍　玮 2，翟　颀 2，魏文康 2，杨彩娟 3]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>林秀银 1
，温肖会 2，吕殿红 2，翟少伦 2，霍　玮 2，翟　颀 2，魏文康 2，杨彩娟 3</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201906017&flag=1]]></guid><cfi:id>133</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Analysis on the Contents of Body Fat, Collagen, Amino Acid and Selenium in Wild Pigs Raised Under Highly Simulated Wild Conditions]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201905015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】 The study was conducted to understand the meat quality of wild pigs that raised in highly 
simulated wild conditions. 【Method】Approaches of soxhlet extraction, hydroxyproline colorimetry, o-phenylenediamine 
ultraviolet spectrophotometry and amino acid automatic analyzer were used to detect the contents of fat, collagen, amino acid 
and selenium in wild pigs cultured under highly simulated wild condition. 【Result】 The results showed that the fat content 
of muscles (mixed with equal mass of meat from the fore bock and back legs ), skin (including subcutaneous fat), large 
intestine and small intestine were 29.8, 417.6, 124.9 and 15.6 mg/g, respectively, and the collagen content of them were 9.03, 
312.21, 18.36 and 19.58 mg/g, respectively. The contents of aspartic acid, glutamic acid and alanine in muscle were 13.5, 
23.7 and 8.40 g/kg, respectively. The contents of selenium in large intestine, small intestine and the mixed meat and skin were 
0.78 , 0.77 and 0.70 mg/kg, respectively. While the selenium content of green fodders was 0.43-1.06 mg/kg. 【Conclusion】The fat content of wild pork was moderate and was rich in collagen, amino acid and selenium.]]></description>
<pubDate>2019/7/1 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[钟春英 1
，刘力源 2，黄舒玮1
，陆祖军1
，宾石玉1
，吴先锋 3]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>钟春英 1
，刘力源 2，黄舒玮1
，陆祖军1
，宾石玉1
，吴先锋 3</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201905015&flag=1]]></guid><cfi:id>132</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Analysis on the Effects of Grass Carp Fed with Different Feed Based on Dike-pond Agriculture]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201905016&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was conducted to explore the differences in growth performance, conventional 
nutrient composition and economic benefits of grass carp cultivated with different feed based on dike-pond agriculture. 
【Methods】A comparative test on grass carp breeding among three groups, i.e. pupa peptide protein group(PP. Group), pupa 
peptide protein feed and jean grass group(PP.+JG.Group), feed from WangHai corp.(WH. Group). 610 grass carps with the initial weight of 770(±11.2) g were selected for each group and were cultured in the test ponds of the same size(0.147 hm2) for 
180 d. At the end of test period, 4 grass carps were randomly selected from each group, and dorsal muscle above the lateral line 
were collected and made into 4 compound samples with a mass of 50(±3.8) g as 4 repeated samples in each group. In addition, the 
dorsal muscle of 4 grass carps with the initial weight was randomly collected before the test to be made into compound samples as 
the blank control group. 【Result】The results showed that the grass carps in PP. Group have the highest weight gain rate (134.3%), 
and the crude fat content in muscle (1.74%) was significantly higher than that of other groups . From the two indicators of weight 
gain rate and specific growth rate, there was an obvious difference among 3 groups: PP. Group >WH.Group>PP.+ JG. Group. The 
crude protein content (20.7%) and delicious amino acid content (6.37%) of grass carps in PP.+JG.Group were the highest, while the 
fat content was the lowest (1.27%), and the input-output ratio was the lowest (0.941). The unsaturated fatty acid content (71.07%) of 
the initial group was significantly higher than that of three test groups (66.98%, 65.48%, 66.07%); while the oleic acid content(C18:1) 
and total amino acid content in the muscle of grass carps in the three test groups were significantly higher than those in the initial 
group, and there was no significant difference in the fish nutritional composition between PP. Group and WH.Group . 【Conclusion】
Grass carps fed with pupa peptide protein feed had a high rate of weight gain, but it was easy to cause body fat deposition. Grass 
carps of PP.+JG.Group grew slowly, but the fish had the highest crude protein content (20.7%) and the lowest fat content (1.27%), 
and higher delicious amino acid content. Raising grass carp with grass based on dike-pond agriculture in the idle areas can 
effectively reduce the cost of feed and improve the fish quality .]]></description>
<pubDate>2019/7/1 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[黄　龙 1
，韦木莲 1
，蒙　烽 1
，吴雅丽1
，廖森泰 2，丁成章 1
，李　强 1
，姜　鹏 3]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>黄　龙 1
，韦木莲 1
，蒙　烽 1
，吴雅丽1
，廖森泰 2，丁成章 1
，李　强 1
，姜　鹏 3</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201905016&flag=1]]></guid><cfi:id>131</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effects of Polyculture of Penaeus monodon“Nanhai No.2”with Sparuslatus and Siganus fuscessens on the Water Quality and Growth of Shrimp in Ponds]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201905017&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】 The objective of the study was to explore the ecological benefits of Sparuslatus and Siganus 
fuscessens in two 1 000 m2 aquaculture ponds for Penaeus monodon. 【Method】 A test was conducted to compare the 
change of nutritive salt, content of particulate material, growth performance of phytoplankton, zooplankton and other indexes in P. monodon“Nanhai No.2”- Sparuslatus (Sparuslatus polyculture treatment, XZ pond) polyculture pond and the change 
of such indexes in P. monodon“Nanhai No.2”- S. fuscessens (S. fuscessens polyculture treatment, XL pond) polyculture 
pond in 105 days. 【Results】 The results showed that the contents of ammonia nitrogen (NH4
+-N), total nitrogen (TN), total 
phosphorus (TP), soluble reactive phosphorus (SRP) and particulate matter in XZ pond and XL pond were increased during 
the entire experiment. At the middle and later experiment period, the dissolved nitrogen in the XZ pond was lower than that 
in the XL pond while the TN content in the XZ pond was higher than that in the XL pond and the difference became more 
significant in the late experiment period. Meanwhile, the particulate matter content in XZ pond was higher than that in the XL 
pond, which indicated that the higher TN content in XZ pond was from suspended particulate matters. The ranges of variation 
of plankton and chlorophyll a content in the two ponds were 6.83-18.65 μg/L and 5.34-16.24 μg/L, respectively, showing no 
significant difference. 【Conclusion】 The experimental results indicated that the bioturbation of Sparuslatus could boost the 
transportation of organic matter from the bottom to the upper body of the pond, which was beneficial to reduce the accumulation 
of NH4
+-N, NO3
--N and other substances, and promote the material circulation in the pond culture system. The survival rate 
of P. monodon“Nanhai No.2”and fish sampling results indicated that the Sparuslatus and S. fuscessens could be cultured 
with P. monodon“Nanhai No.2”directly to achieve higher economic benefits. Further study needs to be conducted on the 
feed filtration capacity of Sparuslatus and S. fuscessens on different phytoplankton with different particle sizes in culture ponds 
because of the importance of phytoplankton in the water.]]></description>
<pubDate>2019/7/1 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[郭晓奇 1
，周发林 2，马志洲 1
，姜 松 2，罗国武 1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>郭晓奇 1
，周发林 2，马志洲 1
，姜 松 2，罗国武 1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201905017&flag=1]]></guid><cfi:id>130</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effects of β-glucan on Growth Performance, Immunity and Antioxidant Indexes of Truogx sinensis]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201904014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The effects of β-glucan on the growth performance, non-specific immunity and antioxidant
indexes of young Truogx sinensis were studied under the condition of green ecological breeding.【Method】180 young Truogx 
sinensis with the body mass of 200 (±10)g were randomly divided into 3 groups. Three tests were performed in each group
and 20 young T.sinensis were tested each time, and fed with basic feed(control group) and test feed with 200 mg/kg (G200)
and 1 000 mg/kg(G1000) doses of β-glucan. After 120 days, the increased weight, the ratio of body weight to viscera and
the ratio of viscera to liver of the young T. sinensis were calculated. The contents of catalase in whole blood, the lysozyme,
alkaline phosphatase, acid phosphatase, superoxide dismutase activity and malondialdehyde in serum were determined. 【Result】 There were no significant differences in increased weight, ratio of body weight to viscera and ratio of viscera to liver
in the tested groups. The Lysozyme activity (LYZ) in the serum of G1000 group was significantly higher than that of the control
group and G200 group while the difference between the G200 group and the control group was not significant. The alkaline
phosphatase(AKP) activity in the G200 group and G1000 group was increased significantly compared with the control group.
The superoxide dismutase(SOD) activity of the G200 group was significantly higher than that of the control group and the G1000
group. No significant difference in acid phosphatase(ACP), catalase (CAT) activity and malondialdehyde(MDA) was observed
in all groups(P >0.05).【Conclusion】 The addition of a proper amount of β-glucan in feed could help to improve the nonspecific immune function and antioxidant level of the young T. sinensis without affecting its growth performance.]]></description>
<pubDate>2019/5/23 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[梁志凌，马艳平，冯国清，郝  乐，马江耀，柯  浩，刘振兴]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>梁志凌，马艳平，冯国清，郝  乐，马江耀，柯  浩，刘振兴</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201904014&flag=1]]></guid><cfi:id>129</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Research Progress on the Application of Low Protein Diets in Pigs at Different Growth Stages]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201904015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[The shortage of protein feed resources, low feed utilization rate and pollution problems have restricted the
development of livestock and poultry breeding industry. Low protein diets balanced with synthetic amino acids could reduce
the use of protein materials such as soybean meal, improve feed utilization rate, and be benefit to sustainable development
of the breeding industry. The application of low protein diets in pigs at different growth stages were summarized in this
article. Based on the previous studies, it was found that low protein diets prepared with metabolizable energy and digestible
energy feeds were prone to cause carcass to become fat, and a net energy-based diet formulation method was recommended.
Reducing the protein level of 1%-4% could improve the growth performance of pigs in each growth stageto some extent, and
the effect of reducing nitrogen emission was remarkable. However, the protein content in the diets of piglets and sows should
not be too low, and a reduction of 1%-3% in low protein diets was suggested in most studies. The addition of branched- chain amino acids L-Leu, L-Val and L-Ile in low protein diets was beneficial to the improvement of growth performance and
immune performance of the organism. Finally, based on these studies, issues related to the current research and application of
low protein were discussed in this paper. It is believed that low nitrogen diets have broad application prospects.]]></description>
<pubDate>2019/5/23 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[邓  盾，王  刚，陈卫东，马现永]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>邓  盾，王  刚，陈卫东，马现永</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201904015&flag=1]]></guid><cfi:id>128</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Optimization of the Culture Medium of Nannochloropsis oculata]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201904016&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Nannochloropsis oculata is a kind of excellent single-celled diet microalgae, and widely
used in aquaculture.The culture medium of Nannochloropsis oculata was optimized in order to improve its growth rate and
reduce the production costs. 【Method】Taking Ningbo University 3# Formula as the basic microalgae culture medium,
with CHCOONa as carbon source, KNO3, (NH2)2CO and NH4CL as nitrogen source, KH2PO4 and NaH2PO4 as phosphorus
source, FeSO4 and FeC6H5O7 as iron source, the effects of different nutrient sources such as carbon, nitrogen, phosphorus,
iron,Vitamin B1 and Vitamin B12 on the growth and propagation of N. oculata were analyzed by one-factor experiment
and orthogonal test. 【Result】The optimized culture medium based on nature sea water was formulated as follows: 3 g/
L CHCOONa, 20 mg/L NH4Cl-N, 2 mg/L KH2PO4-P, 3 mg/L FeSO4-Fe, 0.05 mg/L Vitamin B1 and 0.005 mg/L Vitamin
B12. The N.oculata was cultured on optimized medium and Ningbo University 3# medium and the results showed that the
biomass (cell density) of N. oculata cultured on optimized medium for 2-6 days was 2.21, 2.55, 2.30, 1.97 and 1.7 times higher than that of Ningbo University 3# Medium respectively. By the end of the sixth day, the biomass (cell density) of N. 
oculata on the optimized medium was up to 1.74×107 cell/mL, which was 1.7 times higher than that on Ningbo University
3# medium. 【Conclusion】The optimized medium extrenely enhamced the biomass of N. oculata and it was a good medium
for N. oculata.]]></description>
<pubDate>2019/5/23 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[韩福光 1
，王  珺 1，2，林秋露 1
，王  茜1，2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>韩福光 1
，王  珺 1，2，林秋露 1
，王  茜1，2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201904016&flag=1]]></guid><cfi:id>127</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Research Progress on Porcine Deltacoronavirus]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201903018&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[Porcine Deltacoronavirus (PDCoV) is a member of the δ-coronavirus, a newly discovered coronavirus
in 2012 which can infect porcine. Such coronavirus is clinically characterized by diarrhea symptoms in sows and piglets
and basically characterized by vomiting, watery diarrhea, dehydration and loss of appetite. The mortality rate of the
infected piglets is 30%-40%. The disease outbroke in the United States in 2014. The disease emerged in our country in
the same year and has spread to many countries and regions in the following years, which brought huge economic losses
to pig industry. At present, a series of test methods have been established for PDCoV infection, test results showed that
PDCoV not only infected pigs alone but also mixed with other porcine intestinal coronavirus to infect pigs. However, up
to now, there are few researches on the pathogenic mechanism of PDCoV and the understanding of the disease is not
comprehensive enough. There is still no effective vaccine for preventing and controlling the emergence of the disease.
In this paper, the current research status of PDCoV genome characteristics, protein function, epidemiology, genetic and
evolutionary analysis, detection methods and prevention and control measures were summarized so as to provide reference
for the study of pathogenic mechanism and clinical research of PDCoV.]]></description>
<pubDate>2019/4/24 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[董建国 1
，饶  丹 1
，覃燕灵 2，王艳午 2，张  宁 3，易本驰 1
，黄  立 1
，刘纪成 1
，邓凯伟 1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>董建国 1
，饶  丹 1
，覃燕灵 2，王艳午 2，张  宁 3，易本驰 1
，黄  立 1
，刘纪成 1
，邓凯伟 1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201903018&flag=1]]></guid><cfi:id>126</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Analysis on the Expression of Dmrt Gene in Different Stages of Embryonic Development of Epinephelus coioides]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201903019&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The embryonic development process of the Epinephelus coioides and the expression of
Dmrts gene in this process were studied to provide a basis for further study on the function of Dmrts gene in E. coioides.
【Method】The fertilized eggs obtained by natural spawning method were incubated in a constant temperature (24±0.5℃ )
and in still water, and cultured in seawater with a salinity of 31.0. The whole embryonic development process was
completed in about 29 h 50 min and entered the post-embryonic development stage. The embryonic development process of the E. coioides was observed by light microscopy. The embryos in the cleavage stage, blastocyst stage, gastrula
stage, neurula stage, organogenesis stage and newly hatched larvae stage were stored in RNAlater with 5 parallels in each
group. 【Result】The relative expression of Dmrt in different embryonic development stages were analyzed by fluorescence
quantitative analysis. The results showed that the Dmrt gene of the E. coioides began to express from the cleavage stage
during embryonic development. The expression level of Ecdmrt1 (E. coioides Dmrt1) reached the peak at the blastocyst stage,
and the expression level of Ecdmrt1 gene was relatively low at the cleavage stage and larvae stage. The expression level of
Ecdmrt1 gene at the blastocyst stage was 5.4 times that of cleavage stage. The relative expression of Ecdmrt2 (E. coioides 
Dmrt2) gene was relatively stable at the early embryonic stage but increased significantly at the neurula stage (P<0.05),
which was 9.2 times that of cleavage stage. The Ecdmrt2 gene had the highest expression level in the newly hatched larvae,
which was 17.6 times that of the cleavage stage. The expression level of Ecdmrt2b gene increased gradually during embryonic
development and reached the peak at the organogenesis stage (P<0.05) while the expression level decreased at the newly
hatched larvae stage. The expression level of Ecdmrt3 (E. coioides Dmrt3) was relatively low in the early stage of embryonic
development, and significantly increased at the organogenesis stage (P<0.05), which was 7.1 times that of the cleavage stage,
and reached the peak at the newly hatched larvae stage. 【Conclusion】Ecdmrt gene expressed from the cleavage stage
during embryonic development, and may be involved in the development of embryonic organs and larvae.]]></description>
<pubDate>2019/4/24 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[胡  娟 1
，林芳妹 1
，吕晴霁 1
，王  庆 1
，杨慧荣 1
，石和荣 2，赵会宏]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>胡  娟 1
，林芳妹 1
，吕晴霁 1
，王  庆 1
，杨慧荣 1
，石和荣 2，赵会宏</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201903019&flag=1]]></guid><cfi:id>125</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Evaluation of Salinity-alkalinity Tolerance of F3, F4 and F5 Selected Generations of Oreochromis niloticus]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201902019&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The salinity and alkalinity tolerance of the third, fourth and fifth generation (F3, F4 and F5) of
Nile tilapia (Oreochromis niloticus) was evaluated in order to provide basic data for the salinity and alkalinity breeding of Nile
tilapia.【Method】The acute lethal test method was used to determine the tolerance of salt, alkali (NaHCO3) andsalinityalkalinity to the F3, F4 and F5 generation of Nile tilapia. 【Result】The results showed that under the acute lethal condition,
the semi-lethal concentrations of 96h salinity of F3, F4 and F5 generations were 22.473 g/L, 24.468 g/L and 23.833 g/L,
respectively; while the semi-lethal concentrations of 96h alkalinity were 8.256 g/L, 8.443 g/L and 8.453 g/L, respectively.
In the salinity-alkalinity experiment, when the salinity was 10 g/L, there was no death. When the salinity was 15 g/L, the semilethal concentrations of 96 h alkalinity were 4.612 g/L, 4.538 g/L and 4.83 g/L, respectively. When the salinity was 20 g/L, the semi-lethal concentrations of 96 h alkalinity were 1.861 g/L, 1.875g/L and 1.782 g/L, respectively. Under the acute test, there
was no significant difference between F4 and F5 selected generations in semi-lethal concentrations of salinity, alkalinity and
salinity-alkalinity. Under the acute salinity test, salinity semi-lethal concentrations of F4 and F5 were significantly higher than
those of F3 .Under the acute alkalinity test and the salinity-alkalinity test, the semi-lethal concentrations of F4 and F5 were
not significantly different from those of F3 .【Conclusion】The results showed that the salt and alkali tolerance of F5 selected
generation of Nile tilapia was stable.]]></description>
<pubDate>2019/3/26 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[宋凌元 1
，涂翰卿 1
，周昊天 1
，赵  岩 1
，赵金良 1
，筴金华 2，张艳红 2，任炳琛 2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>宋凌元 1
，涂翰卿 1
，周昊天 1
，赵  岩 1
，赵金良 1
，筴金华 2，张艳红 2，任炳琛 2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201902019&flag=1]]></guid><cfi:id>124</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Analysis on Grey Correlation Between Zooplankton Diversity and Water Quality in Culture Pond Based on Two Kinds of Biological Floating Beds]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201902020&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Taking the culture ponds with two kinds of biological floating beds as the research object,
the diversity of zooplankton in pond and its correlation with water quality factors were studied.【Method】The species
Vcomposition of zooplankton and main water quality factors in the ponds of Pelteobagrus fulvidraco cultured in two kinds of
biological floating beds were monitored regularly and the effects of water hyacinth floating bed and alligator weed floating
bed on the diversity of zooplankton were compared. Grey Relational Analysis was used to analyze the correlation of 10
physicochemical factors of water, such as WT, DO, pH, NH3-N,COD and the Shannon-Wiener diversity index (H) and Pielou evenness index (J) of zooplankton.【Result】The results showed that the abundance and diversity of zooplankton in
water hyacinth floating bed ponds were higher than those in alligator weed floating bed ponds, and the water quality of water
hyacinth floating bed ponds was stable during the culture cycle. The dominant species zooplankton was rotifer. Among the water
quality factors, the pH value was the first factor affecting H and J, with the correlation degree of 0.863, 0.797 and 0.901, 0.825,
respectively, followed by WT and DO. The influence of main water quality factors on H and J ranked as follows: pH > WT > DO
> COD > SD > BOD5 > NH3-N > TN > TP > NO2-N.【Conclusion】Among the many water quality factors, pH is the primary
key factor affecting the diversity of zooplankton, followed by WT and DO. The water hyacinth floating bed can better regulate
the population structure of zooplankton than the alligator weed floating bed.]]></description>
<pubDate>2019/3/26 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[陈  琴 1
，高胜男1
，李恩军 2，程光平 1
，崔  亮 1
，赵学倩 1
，何绪伟 1
，秦秀东 2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>陈  琴 1
，高胜男1
，李恩军 2，程光平 1
，崔  亮 1
，赵学倩 1
，何绪伟 1
，秦秀东 2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201902020&flag=1]]></guid><cfi:id>123</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Research Progress of Behavioral Evaluation on Pig Pain]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201901017&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[With the attention to animal welfare issues, how to reduce the pain of pigs in the process of breeding and 
management is a new topic facing to livestock veterinarians. In the process of pork production , pigs will undergo a series 
of management procedures (such as tooth cutting, castration and tail-cutting, etc.), fighting injuries, lameness caused by 
environmental factors and pain caused by other diseases. With the combination of pig management practices, this paper 
explained the pain occurrence , pain assessment, behavioral changes and quantitative sensory tests in pigs, and summarized 
the methods and behavioral indicators for the evaluation on pig pain. Since there was no unified standard and method for 
animal pain assessment in pigs, this article summarized methods and behavioral indicators for assessing pig pain so as to 
provide references for the development of more effective pain relief drugs and the preparation of relevant strategies.]]></description>
<pubDate>2019/3/12 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[张  亮，邱进杰，郭宗义，涂  志，范治连]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>张  亮，邱进杰，郭宗义，涂  志，范治连</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201901017&flag=1]]></guid><cfi:id>122</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Comparison of Phenotypic Traits and Path Analysis of Three Species of Wild Pearl Oysters]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201901018&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】 The objective of the study was to provide scientific reference for the development of new 
species with excellent production performance and the selection of parents pearl oysters. 【Method】 One hundred wild 
pearl oysters from each of three species (Pinctada fucata, Pinctada nigra and Pinctada radiata) were collected from natural 
sea areas and their morphological traits (shell length, shell height, shell width and hinge length) and main economic traits 
(wet weight, tissue weight and shell weight) were measured. The phenotypic traits were compared, and path analysis and multiple regression were used for data analysis. 【Result】The results showed that the average and maximum values of the 
phenotypic traits of P. fucata were higher than those of P. nigra and P. radiata, and the shell height, shell width, hinge length 
and flesh content of the P. radiata were higher than those of P. nigra. Among the correlation coefficients of all phenotypic traits, 
except for the correlation between the shell height and shell width of the Pinctada radiata, the correlation of traits of other wild 
pearl oysters reached an extremely remarkable level. Among the three species of wild pearl oysters, the morphological traits with 
the maximum direct impact on the wet weight of the P. fucata, P. nigra and P. radiata were shell width (0.362), shell height 
(0.517) and shell length (0.337), respectively. The morphological traits with the maximum direct impact on the tissue weight of 
the P. fucata, P. nigra and P. radiata were shell width (0.379), shell height (0.388) and shell width (0.340) respectively. The 
morphological traits with the maximum direct impact on the shell weight of the P. fucata, P. nigra and P. radiata was shell height. 
【Conclusion】The morphological traits with the maximum direct impact on the wet weight of the three species of pearl oysters 
are different, while the morphological traits with the maximum direct impact on shell weight are the same.]]></description>
<pubDate>2019/3/12 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[邓正华 1
，陈明强 1
，李有宁 1
，赵 旺 1
，郑 兴 2，刘 芊 3，王继金 4，王 雨 1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>邓正华 1
，陈明强 1
，李有宁 1
，赵 旺 1
，郑 兴 2，刘 芊 3，王继金 4，王 雨 1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201901018&flag=1]]></guid><cfi:id>121</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Determination and Comparison of Blood Physiological and Biochemical Indexes Between SPF BALB/c Mice and C57BL/6Mice in Different Physiological Stages and Genders]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201912014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was conducted to measure and compare the blood routine and blood biochemical 
values of 6 weeks old and 9 weeks old BALB/c mice and C57BL/6 mice.【Methods】Blood routine and biochemical 
values were determined by automatic blood cell analyzer and automatic biochemical analyzer.【Results】(1) There were 
extremely significant differences in 2 blood physiological indicators(NE% and LY%) of heterosexual BALB/c mice at the 
same physiological stage , and there were significant differences in 8 indicators (LY%, HGB and etc.) in homosexual mice 
at different physiological stages. There were significant differences in 7 blood physiological indicators (RBC and WBC and 
etc.) of heterosexual C57BL/6 mice at the same physiological stage, and there were significant differences in 6 indicators 
(WBC and NE and etc.) of homosexual mice at different physiological stages. (2) There was no significant difference in 
the biochemical indexes between the heterosexual 6 weeks old mice , with the changes of physiological stages, and there 
were extremely significant differences in 8 blood biochemical indicators (AST, ALP and etc.). There was no significantdifference in the biochemical indexes of heterosexual C57BL/6 mice at the same physiological stage , but there were significant 
differences in 7 blood biochemical indicators (ALP, GLU and etc.) . (3) With the changes of physiological stages, there was 
an increase in the difference in part of blood physiological and biochemical indicators of BALB/c mice and C57BL/6 mice.
【Conclusions】The physiological and biochemical indexes of mice blood are significantly affected by the physiological stage 
and gender of mice.]]></description>
<pubDate>2020/3/4 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[黎桂玲，刘　科，黎雄才，邝少松，王　刚]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>黎桂玲，刘　科，黎雄才，邝少松，王　刚</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201912014&flag=1]]></guid><cfi:id>120</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Research Progress of African Swine Fever Detection]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201911015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[Caused by African Swine Fever Viruses (ASFV), African Swine Fever (ASF) is an acute infectious 
disease with high morbidity and mortality. And domestic and wild boars of all ages could be infected by such disease. 
Due to the complexity of ASFV itself and the insufficient understanding of virulence factors and related protective 
genes, there is no effective vaccines and targeted drugs. Since the first report in August 2018, ASF has brought great 
threat to the green and healthy development of the pig industry in China. The successful control of ASF relies heavily 
on fast and reliable laboratory diagnosis, pig isolation and strict biosecurity measures. The current research progress 
of swine fever detection technology in Africa is reviewed from the aspects of molecular biology and immunology 
detection, among which molecular biology methods include ordinary PCR, fluorescent quantitative PCR, digital PCR, 
and so on, and immunology detection methods include red blood cell adsorption reaction, ELISA, immunotomography 
test paper, immune imprinting, etc. in order to provide a selective reference for the diagnosis of swine fever in Africa 
under different conditionsIn order to provide more options for the diagnosis of ASFV under different conditions, 
the current research progress of ASF detection technologies are review from the aspects of molecular biology and 
immunology detection, among which molecular biology methods include conventional PCR, fluorescence quantitative 
PCR, digital PCR, isothermal amplification, and immunology detection methods include haemadsorption, ELISA, 
immunochromatographic strip and immunoblotting, etc.]]></description>
<pubDate>2020/1/10 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[唐　迪，刘　迪，刘　林，王　众，郑　宁，郑业鲁]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>唐　迪，刘　迪，刘　林，王　众，郑　宁，郑业鲁</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201911015&flag=1]]></guid><cfi:id>119</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effects of Different LED Light Colors on Growth Performanceof Wenchang Chicken during Brooding Stage]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201911016&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Based on the different needs of chickens for light environment at different stages, the optimal 
lighting system was studied. This experiment was conducted to investigate the effects of 7 LED light colors, namely, white, 
warm white, red, yellow, blue, green and purple, on the growth performance of Wenchang chicken during brooding stage, 
aiming to find out the most suitable light color for Wenchang chicken breeding.【Method】A total of 630 1-day-old 
Wenchang chicken (hen) of similar weight were randomly assigned to 7 different light color treatment groups. Each treatment 
included 3 replicated groups with 30 chicks per group. Feed and water were provided for ad libitum under cage culture. Lightwas available 24 hours every day and the test lasted for 8 weeks.【Result】The results showed that the 8-week-old chickens 
treated in blue light had an average body weight of up to 793.16 g, which was 58.68 g, 45.17 g, and 45.77 g heavier than those 
treated in red, white, and green light, showing significant differences. The average body weight gain of chickens in blue light was 
significantly different from that in red light, green light and white light, and the body weight gain of chickens in blue light was 
45.12 g higher than that in white light, while the chickens in red light had the least body weight gain. During the whole period of 
the test, the weight uniformity of Wenchang chicken population in blue light was the best, with an evenness of above 90%. There 
was significant difference in body weight gain uniformity between blue light treatment and green light treatment, The chickens in 
blue light had the best body weight gain uniformity, followed by white light and warm white light treatments. White light treatment 
had the lowest feed conversion rate, which was significantly different from that of yellow light treatment. The chicken mortality was 
not significantly different among treatments.【Conclusion】Considering the growth weight, population weight uniformity and 
feed conversion ratio, compared with white light treatment, LED blue light could significantly promote the growth of Wenchang 
chickens during brooding stage and improve the uniformity of body weight, followed by purple light and yellow light treatments.
While red light inhibited the growth of chickens, and green light treatment had the worst uniformity of body weight.]]></description>
<pubDate>2020/1/10 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[严　霞1
，欧丁僮 2，刘天飞 1
，王　劼 1
，罗成龙 1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>严　霞1
，欧丁僮 2，刘天飞 1
，王　劼 1
，罗成龙 1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201911016&flag=1]]></guid><cfi:id>118</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Study on Immune Effect of Different PRRSVaccine Combinations on Piglets]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201910015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】For the problem of the diversity of PRRSV strain and the ineffectiveness of vaccine 
immunization, the best vaccination scheme for porcine reproductive and respiratory syndrome vaccine was explored. 
【Method】 The study was carried out in three pig farms (A, B and C) and four different combinations of PRRS vaccines 
were used in each farm. Group 1: 14-day-old piglets immunized with PRRS attenuated vaccine and inactivated vaccine. 
Group 2: 14-day-old piglets immunized with PRRS attenuated vaccines and 35-day-old piglets immunized with 
inactivated vaccine. Group 3:14-day-old and 35-day-old piglets immunized with attenuated vaccines. Group 4: 14-dayold piglets immunized with PRRS attenuated vaccines. The effects of the PRRS vaccine against PRRSV were evaluated 
through antibody titer, viremia, and production parameters. 【Result】 By comparing different immunization groups, it was 
found that A2, B3 and C3 had the highest antibody levels and the S/P were 2.50, 2.63 and 2.99, respectively. The positive 
rates of PRRSV nucleic acid in serum A2, B2, B3 and C1 were the lowest, which were 12.5%, 0, 0, 6.3%, respectively.The incidences of A1, B2 and C1 were the lowest, which were 10%, 2% and 8%, respectively. 【Conclusion】 The combined 
immunization of 14-day-old PRRS attenuated vaccine and inactivated vaccine, and 14-day-old PRRS attenuated vaccine 
and 35-day-old inactivated vaccine showed the best immunization effects.]]></description>
<pubDate>2019/12/17 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[蔡汝健，蒋智勇，勾红潮，楚品品，李　艳，宋　帅，徐志宏，李春玲]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>蔡汝健，蒋智勇，勾红潮，楚品品，李　艳，宋　帅，徐志宏，李春玲</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201910015&flag=1]]></guid><cfi:id>117</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Relationship between the Trait of White-partridge Feathersand SLC45A2 Polymorphism in Wenchang Chicken]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201910016&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】In this study, the inheritance of the trait of white-partridge feathers in Wenchang chicken 
was studied and the related genetic variation was identified., which provided references for the utilization of the whitepartridge population and molecular marker-assisted selection of white-partridge feather trait. 【Method】Firstly, the 
inheritance of white-partridge feather trait was studied by reciprocal test. Then, SLC45A2 was selected as a candidate gene 
based on the genetic characteristics of white-partridge feather and results of previous researches. The variation in the exon 
domain of SLC45A2 gene was analyzed by using re-sequencing method. Lastly, the relationship between missense mutation/ nonsense mutation in the exon domain and the trait of white-partridge feather was verified. 【Result】 The results of reciprocal 
test showed that the trait of white-partridge feather was recessive inheritance compared with that of yellow-partridge feather. 
The amplification results indicated that there were five SNPs in SLC45A2 gene, in which three were located in exon domain, 
including two were missense mutation (c.a74g and c.g1154c) and one was synonymous mutation (c.c561t). Finally, the result 
showed that C.G1154C site of SLC45A2 gene was completely linked with the white-partridge feather trait based on missense 
mutation study in Wenchang chicken, Bawang chicken, Qingyuan partridge chicken, Xinghua chicken and Gallus gallus. 
【Conclusion】 The trait of white-partridge feathers showed recessive inheritance and c.g1154c site might be the causation.]]></description>
<pubDate>2019/12/17 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[何艺平 1,2，陈　杰 3，高鑫凤 4，许继国 2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>何艺平 1,2，陈　杰 3，高鑫凤 4，许继国 2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201910016&flag=1]]></guid><cfi:id>116</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Study on the Effect of Regional Micro-biological CuringAgent on the Improvement of Pond Environment]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201910017&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was conducted to explore the mechanism of the action of the regional microbiological curing agent in improving the ecological environment in aquaculture.【Method】The simulated pond ecological 
environment was established in 4 aquarium tanks, in which the mud and water were collected from a healthy pond in 
Nansha town of Guangzhou City (Group 1 and 3), an unhealthy pond from Nansha Town (Group 2), and an unhealthy pond in 
Qingyuan City. 3 Aristichthys nobilis, 2 Oreochromis mossambicus and 2 Pterygoplichthys multiradiatus were cultured in each tank and the scientific feeding management was applied to observe the effects of conserving stuff on water quality factors, 
phytoplankton, protozoa and fish survival rateby using local pond microorganism in Nansha and Qingyuan and pond microbiological curing agent (conserving stuff) made from agricultural sub-products regularly.【Result】With the use of conserving 
stuff, the ammonia nitrogen was greatly reduced in Group 2 and Group 4, and the water quality factors were stable during the 
experiment period. The average phytoplankton density, number of species and diversity index in experimental groups grew 
more rapidly and varied in less narrow range than those in control group, which showed as: Group 4 ＞ Group 1 ＞ Group 3
＞ Group 2,Group 2 ＞ Group 4 ＞ Group 1 ＞ Group 3 and Group 4 ＞ Group 2 ＞ Group 1 ＞ Group 3 on D90 respectively. 
The main species of phytoplankton could be maintained as chlorophyta and diophyta species, and the proportion of single 
dominant species was reduced. The species richness of protozoa increased obviously, and the average density, number of species 
and diversity index of all groups showed as: Group 1 ＞ Group 3 ＞ Group 2 ＞ Group 4 on D60. The survival rate of fish was 
significantly increased, which showed as: Group 1 (85.7%) ＞ Group 2 (57.1%) ＞ average (50.0%) ＞ Group 4 = Group 3 
(28.6%), and A. nobilis, which needs to live in good water condition,was alive in Group 1 and Group 2.【Conclusion】Both 
the local microorganism in conserving stuff and the adjustable C/N ratio of conserving stuff played active roles in improving the 
aquaculture ecosystem and the stability of pond culture system could be maintained in a dynamic balance.]]></description>
<pubDate>2019/12/17 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[刘春花 1，2，梁慧丽1
，孙承文 1，2，陶家发 1
，巩　华 1
，石存斌 1
，吴淑勤 1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>刘春花 1，2，梁慧丽1
，孙承文 1，2，陶家发 1
，巩　华 1
，石存斌 1
，吴淑勤 1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=201910017&flag=1]]></guid><cfi:id>115</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Research Progress in Composting with Black Soldier Fly to Promote the Decomposition of Livestock and Poultry Manure]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202008015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[With the development of livestock and poultry industry, a large amount of manure has been produced, but the traditional methods of excrement treatment can not effectively deposit a large number of livestock and poultry manure. Composting with black soldier fly is a new environmental protection method for the processing of livestock and poultry manure, which can quickly reduce the level of harmful substances in the manure, the level of odor emissions and the dry matter of livestock and poultry manure. At present, there are many researches on composting treatment with black soldier fly at home and abroad, but more attention is paid to increasing the biomass of black soldier fly larvae, and less to the purpose of decomposing manure. The characteristics of livestock manure and the traditional treatment methods of livestock and poultry manure were introduced, and the methods of promoting the decomposition of livestock and poultry manure by composting with black soldier fly were summarized from the following four aspects: controlling the inoculation amount of black soldier fly larvae, adjusting the manure using amounts according to the types of different manure, co-converting manure with black soldier fly and bacteria and regulating the environmental conditions of manure treatment with a view to provide references for the livestock and poultry manure treatment in actual production.]]></description>
<pubDate>2020/9/27 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[LU Lizhu1, DENG Dun2, MA Ping3, HU Xujin4, LI Jianhao2, MA Xianyong2, HU Wenfeng1, HU Bin2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>LU Lizhu1, DENG Dun2, MA Ping3, HU Xujin4, LI Jianhao2, MA Xianyong2, HU Wenfeng1, HU Bin2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202008015&flag=1]]></guid><cfi:id>114</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Breeding of A New Silkworm Variety Yuecan No.11 Resistant to BmNPV]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202008016&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The selection of new BmNPV resistant silkworm varieties suitable for breeding in Guangdong sericultural areas will provide excellent varieties for stable production in rearing season subjected to BmNPV.【Method】The resistant silkworm germplasms were created by successive generation of pathogen stress and space mutagenesis, and then Chinese varieties and Japanese varieties were bred by means of hybridization and systematic breeding. On this basis, the combination of the quaternary hybrid of Chinese strain·Chinese strain× Japanese strain·Japanese strain was carried out. Taking the current silkworm variety Liangguangerhao in Guangdong Province as the control, the laboratory comparison, the resistance test to BmNPV, the laboratory joint identification for two consecutive years and the rural production identification were carried out.【Result】Chinese varieties Fukang and Chun 5N as well as the Japanese varieties Hang 7 and 7532N were bred, and the quaternary hybrid combination Fukang·Chun 5N × Hang 7·7532N was formed, named Yuecan No. 11. The resistance of this variety to BmNPV was significantly higher than that of the control. In the laboratory joint identification test, the comprehensive performance was as follows: Yuecan No. 11 had the same duration as Liangguangerhao, with strong vitality, larva-pupa rate of 97.58%, cocoon weight of 1.712 g, cocoon shell weight of 0.369 g, cocoon shell rate of 21.55%,cocoon weight per 10 000 larvae of 16.874 kg, cocoon shell weight per 10 000 larvae of 3.636 kg, single cocoon filament length of 942 m, reelability of 80.15%, filament size of 2.568 dtex and neatness of 93.5 points.【Conclusion】The silkworms of Yuecan No. 11 grow and develop uniformly with strong resistance to BmNPV, with high cocoon yield and excellent silk quality. The new silkworm variety is easy to reproduce and bred with good comprehensive characters. It passed the identification of new varieties of crops in Guangdong Province in October 2019, and is suitable for breeding in sericultural areas of Guangdong Province all year round.]]></description>
<pubDate>2020/9/27 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[XIAO Yang, LI Qingrong, XING Dongxu, ZHAO Chaoyi, YANG Qiong]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>XIAO Yang, LI Qingrong, XING Dongxu, ZHAO Chaoyi, YANG Qiong</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202008016&flag=1]]></guid><cfi:id>113</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Preliminary Study on Food Composition and Intestinal Allometric Growth Pattern of Squalidus argentatus in Midstream of the Jialing River]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202008017&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objectives】Study on the relationship between food composition and intestinal allogeneic growth of Squalidus argentatus so as to explor of its ecological adaptation.【Method】A total of 46 S. argentatus were collected from the Cangxi reaches in midstream of the Jialing River to analyze their food composition and intestinal allometric growth pattern.【Result】The intestinal circling pattern of S. argentatus was simple, which showed S-shape. The foregut, midgut, and hindgut were different significantly in length, quality and development degree. The results of the allometric growth equation showed that there were allometric growth in the foregut and the whole intestine of S. argentatus, which changed from rapid growth （before the inflection point） to constant growth. While the midgut always maintained rapid growth, and the hindgut maintained constant growth. Diet analysis showed that the main diet of S. argentatus was composed of various food categories, including organic detritus, diatoms, freshwater moule （Limnoperna lacustris）, and chironomidae larvae. Due to the various food sources, the S. argentatus was defined as omnivorous fish. S. argentatus was grouped according to the inflection point of foregut. Before the inflection point, organic detritus （Percentage of weight=76.84%）, chironomidae larvae （21.16%）, and diatoms （1.77%） were the main food for S. argentatus （24 individuals）. However, after the inflection point, organic detritus （90.41%） was the most important food category for S. argentatus （22 individuals）, followed by L. lacustris （6.64%） and diatom （1.18%）.【Conclusion】S. argentatus belongs omnivorous fish, its foregut and whole intestine show allometric growth pattern which may be closely related to the prey digestion and absorption.]]></description>
<pubDate>2020/9/27 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[ZHANG Fubin]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>ZHANG Fubin</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202008017&flag=1]]></guid><cfi:id>112</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Bioinformatics Analysis, Vector Construction and Protein Expression of African Swine Fever Virus CP204L Gene]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202007016&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【 目 的】 非 洲 猪 瘟 病（African swine fever，ASF） 是 一 种 由 非 洲 猪 瘟 病 毒（African swine fever virus，ASFV）导致的，能引起猪高度致死的急性传染病。采用生物学手段制备检测试剂能更快、更有效地监测疫情，对 ASF 疫情的防控尤为重要。【方法】根据 ASFV 的 CP204L 基因 CDS 区序列设计特异性引物并进行PCR 扩增，将目的基因片段与 p EASY-T5 连接构建克隆载体，利用生物信息学软件对蛋白进行生物信息学分析，用 T4 连接酶构建重组载体 pET-32a（+）-CP204L，将成功构建的重组表达载体质粒转入感受态 Rosetta（DE3）中，用 1 mmol/L IPTG、37 ℃诱导表达 8 h 后进行 SDS-PAGE 电泳鉴定蛋白表达。【结果】生物信息学分析结果表明，P30 蛋白是一种主要由无规卷曲 α 螺旋和 β 折叠组成的亲水和稳定蛋白，PCR 扩增及测序比对结果证明成功构建了重组表达载体，SDS-PAGE 电泳验证了在 24.3 ku 处有潜在的 P30 蛋白表达。【结论】通过生物信息学软件在分子层面分析了 P30 蛋白的理化性质及蛋白结构，P30 蛋白的表达为制备 ASFV 血清学诊断试剂及诊断技术奠定了基础。]]></description>
<pubDate>2020/9/8 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[LU Zhongshuang1，XU Huihui2，CAI Weibei1，WU Yue1，YANG Lianhua1，WANG Zeyu1，WANG Nan3，XU Yiming4]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>LU Zhongshuang1，XU Huihui2，CAI Weibei1，WU Yue1，YANG Lianhua1，WANG Zeyu1，WANG Nan3，XU Yiming4</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202007016&flag=1]]></guid><cfi:id>111</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effects of Starter Feed and Pig Manure on the Growth of Hermetia illucens L. Larvae]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202007017&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was to investigate the effect of starter feed on the later growth of newly hatched larvae of Hermetia illucens L., and to explore the optimal ratio of mixed compost of pig manure and wheat bran.【Method】Two groups were set: the control group fed with starter feed（whole wheat bran）at initial incubation stage and the experimental group without starter feed but the mixed wheat bran compost with pig manure contents of 90%, 80%, 60% and 50%. In the experiment, the body length, body width and body weight of the larvae were measured regularly.【Result】In the control group, the growth of body length, body width and body weight of the H. illucens L. larvae gradually increased with the decrease of pig manure content, and reached the best when the pig manure content was 50%. In the experimental group, the growth indicators of larvae showed a trend of increasing first and then decreasing with the decrease of pig manure content, and reached the best when the pig manure content was 80%. Except for the 90% pig manure content, the difference between the control group and the experimental group was not significant. The overall growth indicators of the other groups showed that the control group was better than the experimental group and the difference was significant.【Conclusion】It shows that feeding the newly-hatched larvae of the H. illucens L. with starter feed is more conducive to its later growth, and the mixed compost of pig manure and wheat bran is most beneficial to the growth of larvae when the content of pig manure is 50%.]]></description>
<pubDate>2020/9/8 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[YE Jiawei, GUAN Wanting, SHI Yifu, SHI Yunfeng, TANG Xuelian, FU Jinghua, XU Minjun]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>YE Jiawei, GUAN Wanting, SHI Yifu, SHI Yunfeng, TANG Xuelian, FU Jinghua, XU Minjun</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202007017&flag=1]]></guid><cfi:id>110</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Optimization of Method for Ultra-low Temperature Cryopreservation of Paramisgurnus dabryanus Semen]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202007018&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was to optimize the method for ultra-low temperature cryopreservation of the semen of Paramisgurnus dabryanus.【Method】With the thawed sperm motility as the parameter, the experimental conditions were controlled, the best semen diluent and antifreeze were selected, and the volume fraction of antifreeze and the dilution ratio of semen were optimized.【Result】Among the five diluents, diluent Ⅳ was the most effective, with a frozen sperm motility of 35.5（±2.7）%. For the three antifreeze protectants, namely, glycerol（GLY）, methanol（METH）, and dimethyl sulfoxide（DMSO）, when treated with 5%（V/V）DMSO, the frozen sperm motility was 40.7（±2.5）%, significantly higher than that of the 5% GLY and METH treatments. Optimization of the DMSO volume fraction revealed that 10% final concentration of DMSO was more effective in protecting semen, with a frozen sperm motility of 54.6（±1.5）%. Based on the optimization of dilution Ⅳ and semen dilution, it was found that good results could be achieved when diluent IV was diluted with semen at a volume ratio of 3 ∶ 1, and the frozen sperm motility could reach up to 65.4（±2.3）%.【Conclusion】By setting the volume ratio of diluent Ⅳ to the semen as 3 ∶ 1 and a final concentration of 10% DMSO as antifreeze, the best results were obtained when the semen of P. dabryanus was cryopreserved at ultra low temperature.]]></description>
<pubDate>2020/9/8 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[QIAO Ruifeng1, LU Zhuanling2, GAO Shuangshuang1, LEI Kun1, WEI Youchuan1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>QIAO Ruifeng1, LU Zhuanling2, GAO Shuangshuang1, LEI Kun1, WEI Youchuan1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202007018&flag=1]]></guid><cfi:id>109</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Investigation on Reproduction, Slaughter and Meat Traits of Tibetan Pigs in Guangdong Province]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202006011&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】This study was conducted to understand the reproduction, slaughter, and meat traits of Tibetan pigs which were introduced from high altitude areas and raised in warm and humid plain areas of Guangdong province. These results provide a basis for the exploitation and utilization of resources and breeding of Tibetan pigs in Guangdong province. 【Method】Investigation on Tibetan pigs in three Tibetan pig breeding sites in Guangdong province were conducted during 2018 to 2019.【Result】The litter size of Tibetan sows was 8.38（±1.96）heads, and weaning survival rate of piglets was 92.53（±9.79）%; the birth weight of piglet was 0.75（±0.16）kg, and the average weaning weight was 5.38（±1.18）kg. 
The carcass weight of Tibetan boars was 25.17（±6.14）kg, total lean meat rate was 50.46（±4.54）%, skin rate was 11.90
（±2.70）%, bone rate was 10.30（±1.77）%, fat rate was 25.51（±6.34）%, leaf fat rate was 7.41（±1.51）% and average back fat thickness was 2.80（±0.87）cm. The carcass weight of Tibetan sows was 22.35（±5.42）kg, total lean meat rate was 51.92（±5.73）%, skin rate was 12.57（±4.85）%, bone rate was 10.28（±2.00）%, fat rate was 22.97（±9.04）%, leaf fat rate was 6.19（±1.89）%, and average back fat thickness was 2.11（±0.59）cm. After slaughter, pH1h of Tibetan pigs was 6.25±0.08, pH24h was 5.25±0.08, shear force was 41.92（±1.02）N, drip loss at 24 h was 1.74（±0.12）%, muscle marbling score was 2.93（±0.17）points, and intramuscular fat content was 3.35（±0.53）%. 【Conclusion】The reproductive performance and meat production performance of Tibetan pigs in Guangdong area were improved compared with those of Tibetan pigs in high attitude area, indicating that Tibetan pigs were suitable for breeding in Guangdong. However, the meat production performance and meat quality of Tibetan pigs in Guangdong were significantly lower than those of conventional lean pigs, indicating that there was still much room for improvement in breeding.]]></description>
<pubDate>2020/8/10 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[WANG Suting1, PAN Jie2, HU Bin1, LI Baohong1, DU Zongliang1, WU Zhenfang3, LI Jianhao1, MENG Fanming1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>WANG Suting1, PAN Jie2, HU Bin1, LI Baohong1, DU Zongliang1, WU Zhenfang3, LI Jianhao1, MENG Fanming1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202006011&flag=1]]></guid><cfi:id>108</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Cloning and Prokaryotic Expression Analysis of glyA Gene of Vibrio harveyi]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202006012&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Vibrio harveyi is a normal flora present in seawater, however, it has been one of the reasons for the disease and even death of seawater fish in recent years. The main purpose of this study was to clone and analyze the prokaryotic expression of glyA gene in V. harveyi ZJ0603 strain in order to lay a foundation for the development of subunit vaccine.【Method】The PCR technique was used to clone the glyA gene of V. harveyi strain ZJ0603, and the analysis on the physicochemical properties, signal peptides, subcellular localization and advanced structural of its encoded serine hydroxylmethyltransferase（SHMT）were performed. The obtained glyA gene was connected to the prokaryotic expression vector pET-28a to construct the recombinant plasmid pET-28a-glyA, and then the recombinant strain BL21-pET-28aglyA was constructed. After sequencing, the proper strains were selected and induced with IPTG and Western blot analysis and identification of the recombinant protein were conducted. And the expression conditions of the recombinant strain BL21-pET-28a-glyA were optimized to obtain a large number of proteins.【Results】Bioinformatics analysis showed that the glyA gene had a total length of 1 296 bp, a total of 431 amino acids, a theoretical isoelectric point of 6.18, an instability coefficient of 28.66, and a total average hydrophilicity of -0.200. Generally, the glyA gene was hydrophilic and distributed in the cytoplasm, and the expected molecular weight of the protein was 46.60 ku. The recombinant plasmid pET-28a-glyA was successfully constructed and transferred into the expression strain. After induction by IPTG, Western blot results showed that the SHMT 
recombinant protein was successfully obtained.【Conclusion】The optimal induction time, concentration and temperature of 
IPTG were 4 h, 0.4 mmol/L and 37 ℃ after optimizing the expression conditions by the variable-controlling method.]]></description>
<pubDate>2020/8/10 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[SUN Guorong, MA Shaohong, WEI Guangben, ZHANG Yilin, TAN Huiming, CAI Shuanghu]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>SUN Guorong, MA Shaohong, WEI Guangben, ZHANG Yilin, TAN Huiming, CAI Shuanghu</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202006012&flag=1]]></guid><cfi:id>107</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Epidemiology Investigation on the Pathogens of WSS and EMS of Wild Prawn]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202006013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was to investigate the epidemiology of pathogen of White Spot Syndrome（WSS）and Early Mortality Syndrome（EMS）of wild prawn in natural sea area. 【Method】The detection on the WSS and EMS viruses of wild shrimps collected from Daya Bay during 2018 and 2019 was performed by PCR detection method.【Result】The results showed that the average virus positive rate of WSS was 22.96% during 5 months’detection in 2018, and the virus positive rate was 0 in August. The average virus positive rate of WSS was 2.43% during 9 months’detection in 2019, and the virus positive rate was 0 in January and from August to October. The average virus positive rate of EMS was 29.93% in 2018, with the highest value of 63.04%. The average virus positive rate of EMS was 41.33% in 2019, with the highest value of 94.00%.【Conclusion】The time distribution of virus-carrying rate of WSS and EMS of wild shrimps in Daya Bay seemed to be irregular. The virus-carrying rate of WSS was in a lower level, but that of EMS was in a higher level.]]></description>
<pubDate>2020/8/10 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[LIU Su1, GUO Yixin2, ZHANG Haifa1, YANG Yuqing1, WU Jinhui1, SHI Herong1, HUANG Jinxiong1, HUANG Peiwei1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>LIU Su1, GUO Yixin2, ZHANG Haifa1, YANG Yuqing1, WU Jinhui1, SHI Herong1, HUANG Jinxiong1, HUANG Peiwei1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202006013&flag=1]]></guid><cfi:id>106</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Isolation and Identification and Drug Susceptibility of Vibrio alginolyticus from Green Sea Turtle]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202006014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Rotten skin is a common disease in hatchling breeding of green sea turtles（Chelonia mydas）, with high infectivity, morbidity and mortality. In order to determine the etiology and treatment of the disease, a pathogenic bacterial was isolated from the rotten tissue of flipper of diseased C. mydas.【Method】Suspected pathogens were isolated and obtained from limb lesions of diseased larval turtle and marked as CMRT91026. The morphological, physiological and biochemical characteristics as well as drug susceptibility were analysed. The phylogenetic tree was established on the basis of the identified 16S rRNA.【Result】The results showed that the isolated strain could grow on TCBS medium and the colony was yellow. The physiological and biochemical analysis indicated that the strain CMRT91026 was Gram-negative Brevi bacterium. The biochemical characteristics of the strain CMRT91026 on 1%NaCl phosphate glucose peptone water, sucrose, mannose and lysine decarboxylase media presented positive and it could grow in 3%-10% peptone. The homology of 16S rRNA among CMRT91026 and other typical Vibrio alginolyticus on NCBI was 100%. Thus, strain CMRT91026 was identified as V. alginolyticus. The antibiotic susceptibility test showed that strain CMRT91026 was sensitive to enrofloxacin, nalidixic acid, levofloxacin, minocycline, cefepime and ceftriaxone. It exhibited intermediate sensitivity to doxycycline, flurfenicol, tobramycin, spectinomycin, norfloxacin, aztreonam and resistance to 15 drugs such as neomycin, sulfamethoxazole compound, amoxicillin, gentamicin, amikacin and etc.【Conclusion】The strain is identified as V. alginolyticus and it is sensitive to quinolones（enrofloxacin）, tetracyclines（minocycline）and cephalosporins（ceftriaxone）. The test results contribute to the diagnosis and prevention of V. alginolyticus disease in larval green sea turtles.]]></description>
<pubDate>2020/8/10 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[YE Mingbin1, CHEN Hualing1, FENG Guoqing2, LIANG Zhiling2, DUAN Jinxia1, LIU Zhenxing2, GUAN Yu'an1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>YE Mingbin1, CHEN Hualing1, FENG Guoqing2, LIANG Zhiling2, DUAN Jinxia1, LIU Zhenxing2, GUAN Yu'an1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202006014&flag=1]]></guid><cfi:id>105</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Comparison of Pork Quality Between Two Hybrid Combinations]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202005011&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Two-way crossbred pigs（DZ）of Duroc boars and Tibetan sowsand three-way crossbred pigs（ZDE）of Du' er sows（offspring of Duroc boars and small-ear spotted sows）and Tibetan boarswere used to compare the difference of meat quality.【Method】At the age of 10 months, after fasting for 12 hours, 8 heads（4 males and 4 females）were selected for slaughtering and sampling, and the carcass quality and meat quality traits as well as intramuscular fat, inosinic acid, fatty acid and amino acid contents were measured.【Result】The results showed that:（1）there was no significant difference in carcass quality between DZ and ZDE.（2）Compared with ZDE, the pH45min value of muscle in DZ increased by 4.19%（P ＜ 0.05）, while the L *24h value and shear force decreased by 6.26% and 17.61%, respectively（P ＜ 0.05）.（3）Compared with ZDE, the contents of intramuscular fat and inosinic acid in muscle of DZ increased by 121.59% and 40.88%, respectively（P ＜ 0.05）.（4）Compared with ZDE, linolenic acid and eicosadienoic acid in muscle of DZ decreased by 21.05% and 33.33%, respectively（P ＜ 0.05）, while eicosenoic acid increased by 33.71%（P ＜ 0.05）.（5）Compared with ZDE, the contents of methionine, threonine, glutamic acid, serine and tyrosine in muscle of DZ increased by 58.71%, 45.94%, 176.94%, 36.87% and 48.02%, respectively（P ＜ 0.05）, and the contents of total amino acids, total essential amino acids and total non-essential amino acids in muscle of DZ increased by 22.65%, 16.78% and 24.51%, respectively（P ＜ 0.05）.【Conclusion】It can be concluded that the crossbred pigs of Duroc boars and Tibetan sows have better meat quality and nutritional value.]]></description>
<pubDate>2020/7/15 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[LI Zhenming1,2, YU Miao1, MA Xianyong1, RONG Ting1, MENG Fanming1, LI Baohong1, ZHANG Jie2, LI Jianhao1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>LI Zhenming1,2, YU Miao1, MA Xianyong1, RONG Ting1, MENG Fanming1, LI Baohong1, ZHANG Jie2, LI Jianhao1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202005011&flag=1]]></guid><cfi:id>104</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[A Genome-wide Association Study for Age at Puberty of Duroc Pig]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202005012&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【目的】母猪初情期日龄是重要的经济性状，直接影响母猪的繁殖利用年限，但调控母猪初情期启动的机制尚不清楚。【方法】基于全基因组芯片数据，对 571 头杜洛克母猪初情期日龄进行全基因组关联分析，筛选影响母猪初情期日龄的重要基因。【结果】571 头杜洛克母猪的初情期日龄符合正态分布，初情期日龄最早为 173 d，最晚为 291 d，平均为 224 d；初情期越早的母猪表现出更高的窝产仔数和平均窝重；通过质控，共有 30 281 SNP 位点被用于全基因组关联分析，在前 10 个潜在 SNP 位点附近，找到 ABCC8、BCAR3、NELL2 和 NSF 等基因，这些基因的主要功能富集在 ATP binding、第二性征发育、激素分泌的调控方面。【结论】初步筛选了影响母猪初情期日龄的基因，但具体功能需要进一步研究确认。此研究不仅能为解析母猪初情期启动的遗传机制提供参考，还能为母猪初情期日龄的遗传改良提供一定的理论基础。]]></description>
<pubDate>2020/7/15 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[YUAN Xiaolong1,2, XU Dantong1, CAO Haonan1, XIONG Haoming1, ZENG Jianhua2, CHEN Zitao1, ZHANG Zhe1, LI Jiaqi1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>YUAN Xiaolong1,2, XU Dantong1, CAO Haonan1, XIONG Haoming1, ZENG Jianhua2, CHEN Zitao1, ZHANG Zhe1, LI Jiaqi1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202005012&flag=1]]></guid><cfi:id>103</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Comparison of Different Extraction Methods of Exosomes from Chicken Embryos]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202005013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objectives】In traditional Chinese medicine, chicken embryo eggs were used as medicated diet supplements to improve human immune. The effects of different extraction methods on the isolation of chicken embryoderived exosomes were studied to further analyze the physiological functions of chicken embryo eggs.【Method】12 Huiyang chicken embryo eggs with embryonic age of 13 days were selected (3 groups, 4 eggs/group) and pretreatment was carried out by enzyme digestion, differential centrifugation and filtration, then the obtained samples divided into four parts. Subsequently, the four parts were processed by four different exosomes isolation methods: ultracentrifugation (UC) , sucrose density ultracentrifugation (SDUC) , membrane affinity (exoEasy) and polyethylene glycol precipitation (ExoQuick) and labeled from A to D, respectively. The protein concentration, morphology, size and protein expression of the isolated exosomes were compared by using four methods: BCA protein assay, transmission electron microscopy (TEM) , nano-flow cytometry (nanoFCM) and fluorescence activated cell sorting（FACS）.【Result】The results showed that all the 4 isolation methods could produce extracellular vesicles within the expected size range (40–200 nm) and the vesicles had typical microcapsule structure of exosomes.The exosomal protein concentration and particle concentration varied under different isolation methods (D ＞ C ＞ A ＞ B) , in which D contained other aggregate-contaminating proteins. Flow cytometry confirmed the expression of two marker proteins, CD63 and CD81, in samples isolated by all methods (B ＞ D ＞ C ＞ A) .【Conclusion】Considering the exosomes purity, morphology, size and expression of the marker protein comprehensively, the sucrose density ultracentrifugation or membrane affinity methods may be preferred according to the source of tested samples, experimental conditions and purposes. The sucrose density ultracentrifugation is preferred when high-purity exosomes are required, while the membrane affinity method is recommended for limited samples and the unavailability of ultracentrifugation equipment.]]></description>
<pubDate>2020/7/15 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[LI Ying, QU Hao, HE Jingyi, LIU Tianfei, WANG Jie, WANG Yan, SHU Ding-ming, LUO Chenglong]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>LI Ying, QU Hao, HE Jingyi, LIU Tianfei, WANG Jie, WANG Yan, SHU Ding-ming, LUO Chenglong</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202005013&flag=1]]></guid><cfi:id>102</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effects of Different Biochar on Copper Forms in Soil and Absorption and Transfer Enrichment of Corn in Sewage Irrigation Area]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202004009&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The effects of biochar from mushroom bran on the forms of heavy metal copper and the absorption and transfer enrichment of corn in sewage irrigation area were studied, and horizontal comparison was conducted on the effects of three kinds of mushroom bran biochar, corn stalk biochar and rice husk biochar.【Method】The forms of heavy metal copper, the heavy metal contents of various parts of corn and the transfer and enrichment coefficients of different parts of the corn to heavy metal copper were analyzed by the application of five kinds of biochar in the soil of a sewage irrigation area in Shanxi through pot experiment.【Result】Under five different biochar treatments, the exchangeable copper content in the soil was lower than that of the blank control. Among them, the EXC-Cu content in the soil was the lowest under LC treatment, which was 1.005 mg/kg, 31.4% lower than the blank control. The CA-Cu content in the soil of all treatments was reduced compared with that of the blank control. Under the HC treatment, the reduction of CA-Cu content was the maximum and the value reduced to 5.91 mg/kg, which was reduced by 40.65% compared with that of the blank control. Except that the R2O3-Cu content under HC treatment increased by 2.25% compared with the blank control, R2O3-Cu content in soil of the other four treatments decreased slightly. OM-Cu content changed  inconsistently. RES-Cu content of all treatments was increased compared with that of the blank control. Under the HC treatment, the increase of RES-Cu content was the maximum, which was 104.55 mg/kg, 15.76% higher than that of the blank control. The transfer coefficients of corn to copper under various treatments were all less than 1, while the enrichment coefficients changed differently.【Conclusion】All of the five different biochar could increase the inactive Cu content and decrease the active Cu content in the soil, which provided some scientific basis for the effective utilization of biochar and the decrease of the effectiveness of Cu content in the heavy metal contaminated soil.]]></description>
<pubDate>2020/6/16 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[TIAN Ye1，CHENG Hongyan1, OH Kokyo2, YAN Shuangdui1, 
CAO Yanzhuan1, HAO Qianping1, LI Danyang1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>TIAN Ye1，CHENG Hongyan1, OH Kokyo2, YAN Shuangdui1, 
CAO Yanzhuan1, HAO Qianping1, LI Danyang1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202004009&flag=1]]></guid><cfi:id>101</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Deodorization Technology of Livestock and Poultry Scale Farms]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202004015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[The sources of odors in livestock and poultry farms are diverse and the components are complex. With the large-scale and intensive development of livestock and poultry breeding, the pollution of livestock and poultry manure is becoming increasingly serious. However, with the development of animal husbandry and the improvement of people’s environmental protection awareness, the environmental requirements for large-scale livestock and poultry farms are getting higher, and the livestock and poultry breeding industry should shift to green production modes. In order to reduce the pollution of manure to the environment and the harm to humans and animals, it is particularly important to treat the manure of livestock and poultry appropriately. Therefore, it is of great significance to study the odor and emission reduction regulation technology of large-scale livestock farms. This article introduces the research progress in the sources, main components, hazards and reduction control technologies of malodorous gases in large-scale livestock farms. It focuses on the malodorous ectopic control technology and in situ control technology. It is believed that the microbial deodorization technology has evident advantages in livestock and poultry production, with strong ability to adapt to the environment, wide application range, and long-lasting deodorization effect. Microbes can greatly degrade the ammonia nitrogen, hydrogen sulfide and other harmful substances produced by the manure, and eliminate the odors in the breeding farm. Although the biological deodorization method has many advantages, it has certain limitations to the treatment of some high-concentration malodorous substances. In order to promote the sustainable development of China’s largescale livestock and poultry breeding farms, the solving of odor problems cannot rely on a single deodorization technology, while the comprehensive use of multiple deodorization technologies can achieve better results only.]]></description>
<pubDate>2020/6/16 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[TANG Yantian1,2, DENG Dun1, LI Zhengming1, YU Miao1, CHEN Weidong1,2, MA Xianyong1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>TANG Yantian1,2, DENG Dun1, LI Zhengming1, YU Miao1, CHEN Weidong1,2, MA Xianyong1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202004015&flag=1]]></guid><cfi:id>100</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Adjustment of Forage Planting Pattern in South China]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202004016&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[As an important component of feed for herbivores, forage is the key material guarantee to obtain highefficient breeding. Combined land location with cultivation time, an appropriate adjustment of the planting pattern of forage is helpful to improve the utilization efficiency of land resources. On the other hand, it can provide maximum guarantee to balance the forage supply all year round. Therefore, scientific configuration of an appropriate planting pattern is particularly important in forage production in South China. Combined with the practical situation of forage production, the forage planting patterns in different locations and different periods are analyzed, providing new ideas for the promotion of forage planting patterns in different regions and the adjustment of forage industrial structure in South China. The main superior forage varieties in the tropic and subtropic regions are further introduced, and their excellent growth characteristics and planting characteristics are summarized. At the same time, combined with relevant researches, the growth performance and nutritional value are evaluated to provide references for the adjustment of forage planting mode. In South China, gramineous grass should be planted in a single mode in combination with the rotation of forage-early rice-late rice, while leguminous forage should be planted in an intercropping mode of fruit（forest）and grass to maximize land conservation.]]></description>
<pubDate>2020/6/16 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[DING Diyun1, SUN Hao1, CHEN Weidong1, WANG Gang1, WU Linying1, CHEN Sanyou2, LI Pinhong2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>DING Diyun1, SUN Hao1, CHEN Weidong1, WANG Gang1, WU Linying1, CHEN Sanyou2, LI Pinhong2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202004016&flag=1]]></guid><cfi:id>99</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Study on the Ovulation of Epinephelus fasciatus Induced by Exogenous Human Chorionic Gonadotrophin Hormone]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202004017&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】This study aimed to understand the effects of exogenous human chorionic gonadotropin（HCG）hormone inducing ovulation of the blacktip grouper（Epinephelus fasciatus）.【Method】The mature E. fasciatus was used as the target object, and the body length, body weight, and condition factor were measured and calculated. After that, 6 female fishes were injected with HCG（dosage: 200 IU/kg or 500 IU/kg）from July to August in 2018 to conduct maturation induction.【Result】Under the 200 IU/kg HCG treatment, before 24 h of the HCG injection, the tertiary yolk stage 1（Ty1）was detected in oocytes of 5 female fishes. After 48 h of injection, the migratory nucleolus oocytes were found in 2 female fishes, and the tertiary yolk stage 2（Ty2）was detected in oocytes of 1 female fish. After 60 h of injection, the ovulation phenomenon was observed in 1 female fish, and the Ty2 was found in other 5 female fishes. In the ovulation individual of 200 IU/kg HCG treatment, the oocyte diameter was 477.0 μm during the injection while it increased to 624.4 μm after 60 h of injection. Furthermore, after the steps of injection, ovulation, fertilization and hatching, the total number of eggs obtained was 16906, and the fertilization rate and hatching rate were 68.7% and 43.0%, respectively. However, the ovulation of E. fasciatus was 
not observed in the 500 IU/kg HCG treatment. 【Conclusion】In conclusion, the 200 IU/kg usage of exogenous HCG hormone treatment may induce the maturation and ovulation in the blacktip grouper. However, to improve the fertilization rate and hatching rate, the injected dosage and effective time should be further regulated.]]></description>
<pubDate>2020/6/16 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[XU Wengang1, TANG Yongzheng1, ZHANG Jianbai2, SOYANO Kiyoshi3, ZOU Huafeng4]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>XU Wengang1, TANG Yongzheng1, ZHANG Jianbai2, SOYANO Kiyoshi3, ZOU Huafeng4</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202004017&flag=1]]></guid><cfi:id>98</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Research Progress in Effects of Matrix Metalloproteinases MMP2 and MMP 9 on Follicular Development in Mammals]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202003014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[In mammalian follicular development, it is accompanied by significant tissue remodeling, which includes 
follicular cavity formation and expansion, ovulation, luteinization and atresia. In the extracellular matrix（ECM）and 
follicular fluid derived from follicular membrane cells and granulosa cells, there are abundant proteins such as gelatin 
protein, fibrin and type Ⅳ collagen. These active proteins provide necessary conditions for the migration, proliferation and 
differentiation of follicular membrane cells and granulosa cells. Under the coordination control of the other MMPs, estrogen, 
apoptosis and autophagy signaling pathways, MMP2 and MMP9 decompose proteins such as gelatin protein, fibrin and type 
Ⅳ collagen in extracellular matrix and follicular fluid, promote cell apoptosis and angiogenesis, degrade and reshape ovarian 
tissues, and then affect the function of follicular membrane cells and granulosa cells, the formation and expansion of follicular 
cavity, and regulate the growth and development of follicles. This paper mainly reviews the research progress of MMP2 and 
MMP9 in regulation of follicular growth, ovulation, luteinization and atresia.]]></description>
<pubDate>2020/6/1 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[孔亚茹，周小枫，熊浩铭，张　哲，张　豪，李加琪，袁晓龙]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>孔亚茹，周小枫，熊浩铭，张　哲，张　豪，李加琪，袁晓龙</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202003014&flag=1]]></guid><cfi:id>97</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Heritability of Egg Production of Chickens: A Meta-analysis]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202003015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Egg production is a major selected trait in the breeding of layer and broiler breeder. A number 
of researches and reports on the heritability of egg production have been conducted, however, the results were discrepant, 
which resulted in the tough choice of breeders. The meta-analysis aimed to evaluate the heritability of egg production of 
layers, broiler breeders and local chickens and the dynamics on the heritability.【Method】Relevant documents on the 
evaluation of heritability of egg production published at home and abroad from 1990 to 2019 were collected by searching 
the databases of CNKI, ISI web of science, PubMed, EBSCO and ProQuest. A total of 72 papers were included in the meta analysis. The dataset of the heritability of egg production was assessed with random effect model, subgroup analysis and two stepwise regression analysis.【Result】Random effect model showed that the heritability of egg production was 0.234±0.009 
and there were great differences between different studies. Subgroup analysis showed that the heritability of white shelled 
layers and brown shelled layers was 0.242±0.022 and 0.224±0.017, respectively, and that of broiler breeders and local 
chickens was 0.221±0.029 and 0.240±0.014, respectively. The heritability showed a declining trend during late laying 
period. Regression analysis showed that the heritability function expressed as: h2=-0.0023t+0.2253.【Conclusion】A small proportion of egg production variation could attribute to the genetic factors, and the genetic background and week age might 
contribute to the heterogeneity between different studies.]]></description>
<pubDate>2020/6/1 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[郭　军，曲　亮，窦套存，王星果，胡玉萍，王克华]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>郭　军，曲　亮，窦套存，王星果，胡玉萍，王克华</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202003015&flag=1]]></guid><cfi:id>96</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effects of a Small Amount of Eggshell Powder Instead of Stone Powder on Laying Performance, Egg Quality and Calcium Metabolism in Laying Ducks]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202003016&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】This study was conducted to determine the effects of a small amount of eggshell powder 
instead of stone powder on laying performance, egg quality and calcium metabolism of laying ducks.【Method】Eighty 
healthy 180-day-old ducks were randomly divided into two groups（control group and test group）. There were 5 replicates 
in each group and 8 duck eggs in each replicate. The control group was fed with normal basal diet, and the test group was 
fed with basal diet among which 2 g calcium was replaced by the same amount of eggshell powder. The test period lasted for 
56 days. The laying performance, egg quality, calcium deposition, calcium and phosphorus contents in blood and levels of 
calcium metabolism-related hormones were measured during the experiment.【Result】The average egg weight, eggshell 
strength and protein height of the test group were significantly higher than those of the control group, which showed that adding eggshell powder could obviously improve egg quality. The levels of calcium deposition, serum phosphorus and plasma 
parathyroid hormone（PTH）in the test group were significantly higher than those in the control group. The blood calcium 
content of the test group 4.74（±1.17）μmol/mL was extremely significant higher than that of the control group 2.79（±1.00）
μmol/mL, showing that eggshell powder was not only more conducive to calcium deposition, but also significantly increasing the 
contents of calcium and phosphorus as well as the levels of parathyroid hormone in blood.【Conclusion】The results indicate 
that a small amount of eggshell powder replacement can significantly promote calcium metabolism and calcium deposition, 
increase the average egg weight and improve the egg quality.]]></description>
<pubDate>2020/6/1 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[江丹莉 1,2，张焯深 2，孙俊峰 2，陈映霓 2，潘建秋 1,2，黄运茂 1,2，沈　栩 1,2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>江丹莉 1,2，张焯深 2，孙俊峰 2，陈映霓 2，潘建秋 1,2，黄运茂 1,2，沈　栩 1,2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202003016&flag=1]]></guid><cfi:id>95</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effects of Mulberry Leaf on Animal Health andIts Application in Animal Production]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202002017&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[As a source of traditional Chinese medicine, mulberry has anti-inflammatory and antimicrobial effects, 
which is associated with its various bioactive components including polysaccharides, polyphenols and alkaloids in different 
parts. Mulberry leaf receives the most attention because it is easy to be harvested and has large biomass. Bioactive 
components in mulberry leaf such as polysaccharides and polyphenols, etc. have also been shown to be antidiabetic and 
antihyperlipidemic and can enhance immunity and antioxidant capacity. Therefore, mulberry leaf and its extract can be 
beneficial to animal health when applied to animal production. With high crude protein contents and balanced amino acid 
composition, mulberry leaf can be used in animal feeds to substitute parts of protein sources. However, there are large 
amounts of anti-nutritional factors in mulberry leaf. Therefore, mulberry leaf undergone fermentation and silaging may be 
more suitable for animal feeding. This paper reviews the effects of these bioactive components on glucolipid metabolism, 
antioxidant activity and immunity of animals, and elucidates the roles of mulberry leaves in improving meat quality,modulating immunity and enhancing antioxidant activity of animals through summarizing the research progresses of mulberry 
leaves in livestock and aquaculture production.]]></description>
<pubDate>2020/4/26 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[林　森 1
，罗国庆 1,2，王振江 1,2，戴凡炜 1,2，赵超艺 1
，唐翠明 1,2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>林　森 1
，罗国庆 1,2，王振江 1,2，戴凡炜 1,2，赵超艺 1
，唐翠明 1,2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202002017&flag=1]]></guid><cfi:id>94</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Research Advances in Antioxidant Function of YeastSelenium and Its Effect on Meat Quality of Finishing Pigs]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202001016&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[Pork consumption plays a leading role in the meat consumption of Chinese residents. With the improvement 
of people's living standards, consumers put forward higher requirements for pork quality. Researches have found that pork 
quality of pigs can be influenced by genetics, nutrition, feeding management and environmental factors, which can be 
reflected by many indicators such as meat color, pH, intramuscular fat, drip loss, tenderness, flavor and so on. The oxidative 
stress is the main factor affecting the pork quality. Selenium is an important part of glutathione peroxidase, which can resistoxidative stress by reducing the production of organic hydroperoxides. Many studies have shown that dietary supplementation 
with yeast selenium can effectively improve the antioxidant capacity of finishing pigs, and improve the meat color, pH value and 
water holding capacity, thus contributing to the improvement of meat quality and extending the shelf life of pork. Therefore, this 
paper reviews the antioxidant mechanism of yeast selenium and its effect on meat quality in finishing pigs, which may provide 
scientific basis for the nutritional strategies concerning improvement of meat quality.]]></description>
<pubDate>2020/4/1 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[杨景森 1
，王其龙 1
，王 丽 2，朱 翠 1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>杨景森 1
，王其龙 1
，王 丽 2，朱 翠 1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202001016&flag=1]]></guid><cfi:id>93</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Research Progress in Fermented Tree Leaves and Its Application in Chicken Production]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202001017&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[Tree resources are rich and diverse in China, but they has not been fully developed and utilized. The 
contents of crude protein, neutral detergent fiber and acid detergent fiber in tree leaves are high, as well as the contents of 
flavonoids and phenols. However, some leaves contain anti-nutritional factors such as tannin and phytic acid. Microbial 
fermentation prolongs the use of leaves, degrades anti-nutritional factors, and transforms nutrients that are difficult to 
be absorbed. It is one of the green feed processing technologies that can change the leaves into biological feed with goodpalatability, non-toxicity, low toxicity and high nutritional value. At present, the main types of fermented leaves are ginkgo 
leaves, moringa leaves, and mulberry leaves. The strains used are mainly lactic acid bacteria, mould, bacillus and yeast. After 
fermentation, the preservation time of the leaves is prolonged, the crude protein content is improved, and the quality is better. 
Fermented leaves have higher contents of flavonoids, amino acids, reducing sugars, polyphenols and other active substances, 
which have strong antioxidant activity. The application of fermented leaves in chicken production can improve the antioxidant 
capacity of animals, promote body and intestinal health, and improve growth performance and production quality. Fermented 
tree leaves used for feeding animals are beneficial for resource recycling, which reduce production costs and solve environmental 
pollution.]]></description>
<pubDate>2020/4/1 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[崔艺燕，田志梅，邓　盾，刘志昌，容　庭，李家洲，马现永，陈卫东]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>崔艺燕，田志梅，邓　盾，刘志昌，容　庭，李家洲，马现永，陈卫东</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202001017&flag=1]]></guid><cfi:id>92</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effects of Sperm Counts on In Vitro Fertilization and Early Embryonic Development of Mice]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202001018&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The effects of different sperm counts on in vitro fertilization and early embryonic development 
of mice were studied, and the assisted reproductive technique of mice was further improved by comparative analysis.
【Methods】In the test, sperms obtained from the epididymal tail of 9 weeks old C57BL/6 male mice were randomly selected 
and put into HTF culture medium, which cultured in carbon dioxide incubator for 1 hour. The sperm counts of semen solution 
of 1, 3, 5, 10, 15 and 20 μL were calculated, the corresponding semen solution absorbed into the oocyte clusters which 
obtained after the superovulation of 4 weeks old C57BL/6 female mice, and the in vitro fertilization rate and the development 
rate of 2-cell embryos calculated after in vitro culture for 24 hours.【Results】Sperm counts of 1, 3, 5, 10, 15 and 20 μL 
semen solution were 0.25 (±0.23)×106, 1.23 (±0.31)×106, 2.03 (±0.35)×106, 3.95 (±0.33)×106, 6.50 (±0.76)×106, 
and 9.88 (±0.52)×106 (units/mL). At the same time, the in vitro fertilization rates of mice were 88.92%, 95.17%, 94.29%, 
90.56%, 85.89% and 78.26%, respectively, and the development rates of 2-cell embryos were 78.21%, 81.75%, 81.51%,77.42%, 70.47% and 50.76%, respectively. The in vitro fertilization rates and the 2-cell embryo development rates with 
sperm counts of 1.23 (±0.31) ×106 and 2.03 (±0.35)×106 (units/mL) were better than those of others, with no significant 
difference,but were extremely significantly higher than those with sperm counts of 6.50 (±0.76)×106, 9.88 (±0.52)×106 (units/
mL).【Conclusions】Various sperm counts have significantly different effects on the in vitro fertilization and early embryonic 
development of mice. The appropriate sperm numbers were 1.23 (±0.31)×106-2.03 (±0.35)×106 (units/mL) , which can 
effectively improve the in vitro fertilization rates of mice and promote the normal development of embryos.]]></description>
<pubDate>2020/4/1 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[黎桂玲，刘　科，杨　林，黎雄才，邝少松，王　刚]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>黎桂玲，刘　科，杨　林，黎雄才，邝少松，王　刚</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202001018&flag=1]]></guid><cfi:id>91</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Prokaryotic Expression and Purification of JAK3 Protein in Golden Pompano (Trachinotus ovatus)]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202001019&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The purpose of this study was to obtain purified recombinant protein of Trachinotus ovatus
JAK3 (TroJAK3)to lay a foundation for understanding the function, interaction and antibody preparation of TroJAK3 protein.
【Method】In this study, the recombinant plasmid pET-32a-TroJAK3 was constructed by molecular biology,the BL21 
competent cells of E. coli were transformed, and. SDS-PAGE and Western blot were used to detect the expression of 
TroJAK3 induced by IPTG.【Result】The PCR amplification fragment was 3 333 bp in length, and the recombinant plasmid 
pET-32a-TroJAK3 was successfully constructed, which was confirmed by double enzyme digestion, nucleotide sequencing 
and open reading frame. After being induced with a final concentration of 1 mmol/L IPTG at 37℃ for 4 h, SDS-PAGE assay 
showed that TroJAK3 recombinant protein was highly expressed in a form of inclusion body, with a molecular weight of about 
140 ku.The purified recombinant protein was obtained by Ni-IDA resin column. Western blot detection showed that here 
was a band of 140 ku, indicating that TroJAK3 could be recognized by anti-his antibodies.【Conclusion】The recombinant 
plasmid pET-32a-TroJAK3 was successfully constructed and purified to obtain TroJAK3 fusion protein with high purity.]]></description>
<pubDate>2020/4/1 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[杨　萌1
，高爽爽 1
，谢业扬 1
，段家文 1
，陆专灵 2，韦友传 1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>杨　萌1
，高爽爽 1
，谢业扬 1
，段家文 1
，陆专灵 2，韦友传 1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202001019&flag=1]]></guid><cfi:id>90</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Comparison of Slide-preparing Methods of Chicken Bone Marrow Dendritic Cells by Indirect Immunofluorescence]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202010016&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】In order to improve the laboratory techniques and methods of dendritic cells（DC）, two slide-preparing methods（cell climbing and cell dropping）of DC from chicken bone marrow by indirect immunofluorescence was compared and analyzed.【Method】Using chicken embryos as test material, the immature bone marrow cells were isolated and obtained from chicken in vitro, and then were activated by Newcastle disease virus（NDV） lastota strain. The expressions of endogenous protein ROBO2（Roundabout） and exogenous protein NDV were monitored. Chicken NDV antibody and mouse ROBO2 monoclonal antibody were hatched together and marked with the combination of FITC. Fluorescence imaging of dendritic cells was examined by laser scanning confocal microscopy（LSCM）, and the difference between cell climbing and cell dropping was analyzed variously.【Result】The cell dropping process was time- consuming and labor intensive, while the cell climbing process was characterized by easy operation and reduced time. NDV and ROBO2 of these two methods showed highly significant differences in single positive and negative and significant influorescence reactivity differences in double positive.【Conclusion】The immunoreactivity of the protein was better with the cell dropping, which was suitable for labeling target and subcellular localization. The morphological and protein characteristics were always 
maintained by the cell climbing,which was beneficial to the fluorescence overlap of the two antibodies and to make the overall color rendering stronger.]]></description>
<pubDate>2020/12/28 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[HE Jingyi, SHU Dingmimg, LIN Chuxiao, LI Ying,WANG Yan, LUO Chenglong]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>HE Jingyi, SHU Dingmimg, LIN Chuxiao, LI Ying,WANG Yan, LUO Chenglong</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202010016&flag=1]]></guid><cfi:id>89</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Transcriptome Sequencing Reveals Gene Expression Differences in Breast Muscle Tissue of Muscovy Ducks with Different Growth Rates]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202010017&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Transcriptome sequencing technology was used to analyze the gene expression differences in Muscovy ducks with different growth rates, and to explore the genes and signaling pathways that affect the growth performance of Muscovy ducks, providing a theoretical basis for elucidating the genetic mechanism of the differences in growth performance.【Method】The breast muscle tissues of two-tailed Muscovy ducks with different growth rates were selected for transcriptome sequencing, and the sequencing results were analyzed by using analysis methods without reference genomes to obtain differentially expressed genes（DEGs）. Blast2 GO software was used to analyze the functional enrichment of DEGs, and the KEGG pathways enrichment of DEGs was analyzed with the KAAS website.【Result】Transcriptome sequencing analysis shows that there are 186 significant DEGs in the fast-growing Muscovy ducks and the slow-growing Muscovy ducks, of which 49 up-regulated and 137 down-regulated. These DEGs are mainly enriched in phosphatidylinositol 3-kinase-serine/threonine kinase（PI3K-AKT）signaling pathway, mitogen-activated protein kinase
（MAPK）signaling pathway and adenylate-activated protein kinase（AMPK）signaling pathway.【Conclusion】The PI3KAKT, MAPK and AMPK are important regulatory signaling pathways that control the growth and development of Muscovy ducks.]]></description>
<pubDate>2020/12/28 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[ZHANG Siyu1, ZHU Weijian2, JI Congliang2, GUO Lijin1, ZHENG Ming1, XU Haiping1, NIE Qinghua1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>ZHANG Siyu1, ZHU Weijian2, JI Congliang2, GUO Lijin1, ZHENG Ming1, XU Haiping1, NIE Qinghua1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202010017&flag=1]]></guid><cfi:id>88</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effects of Dietary Protein Levels on Growth, Physiological Indexes and Body Composition of Ridgetail White Prawn（Exopalaemon carinicauda）]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202010018&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was conducted to explore the optimal protein level in the feed for Exopalaemon carinicauda cultured in ponds.【Method】The experiment was carried out in 12 cement ponds（length×width×depth = 14 m × 12 m × 1.2 m）, and 1 kg of the parents of E. carinicauda with similar specifications（average: 2.5 g/tail） were placed in each pond with a size of 400 tails（half male and half female）. Every 3 cement ponds were set as a treatment, and then were fed with different feed containing 30%, 34%, 38% and 42% of protein （marked as Group 1#-4#, respectively）. The breeding cycle was 4 months, and the body weight, hepatopancreas index（HSI）, fillet yield, physiological and 
biochemical indexes of E. carinicauda of each feed group were measured.【Results】（1）There was no significant difference in body weight, HSI and fillet yield of E. carinicauda among the four feed groups. The body weight and HSI of Group 2# were slightly higher than those of the other groups.（2）The activities of glutamic-oxalacetic transaminase（GOT）and superoxide dismutase（SOD）in the hepatopancreas of each feed group were significantly different. Among them, Group 3# had the lowest GOT level and Group 2# had the highest SOD level.（3）The protein level in the feed had a significant effect on the ash content in the hepatopancreas, and the ash content of Group 3# was significantly higher than that of Group 1# and Group 2#. （4）The feed protein level had a significant effect on the crude fat content and ash content in the muscle. With the increase of the protein level in the feed, the crude fat content in the muscle showed a significant downward trend, while there was a significant increase 
in the ash content in the muscle of Group 1#-3#, among which the crude fat content of Group 1# and the ash content of Group 3# were the highest.【Conclusion】Under the pond culture conditions, the optimal dietary protein level for E. carinicauda was about 34%.]]></description>
<pubDate>2020/12/28 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[FAN Chenwei1，JIANG Xiaodong1，HE Xianlin1，WU Xugan1,2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>FAN Chenwei1，JIANG Xiaodong1，HE Xianlin1，WU Xugan1,2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202010018&flag=1]]></guid><cfi:id>87</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Research Progress of Timed Artificial Insemination in Sow Reproduction]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202108013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[The demand for intensive, large-scale and effective breeding pattern is growing in pig breeding industry. 
Traditional estrus detection and insemination methods are time-consuming and expensive while more likely to be delayed 
in sow reproduction. Timed artificial insemination（TAI）is an innovative technology combining the artificial insemination and estrus synchronization. It helps to regulate reproductive hormones secretion and the follicular development in sows by 
adding exogenous synthetic hormones to make sows show estrus, ovulate and be inseminated during a given time. Moreover, 
this technology contributes to reducing the dependence on estrus detection, production loss caused by recessive estrus and 
crossing infection, and helps increase sows’reproduction frequency per year and reproductive rate as well as facilitate 
epidemic prevention. However, TAI has been introduced into China for a short time, and it still has many problems, including 
unstable effects and insignificant increase in the number of embryos, which need further researches and solutions. This article 
summarizes the regulation law of reproductive hormones in sows as well as the ways to develop TAI for sows at different stages 
of production, and analyzes the application effects of various hormones and key factors affecting hormone effects to provide a 
reference for optimizing TAI procedure and improving reproductive management level for sows.]]></description>
<pubDate>2021/9/24 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[辛海云，李宝红，孟繁明，王塑天，胡　斌，李剑豪，杜宗亮]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>辛海云，李宝红，孟繁明，王塑天，胡　斌，李剑豪，杜宗亮</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202108013&flag=1]]></guid><cfi:id>86</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Construction of FAT1-FAT2 Gene Targeting Vector and Identification of its Expression and Function in Porcine Kidney Cells]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202108014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Polyunsaturated fatty acids（PUFAs）play a vital role in human medicine and nutritional 
value. With the development of biotechnology, it is possible to change the variety and content of unsaturated fatty acid in 
pork by transgenic technology to synthesize PUFAs by mammalian itself, which contribute to the human nutrition and health.
【Method】By cloning FAT1 and FAT2 genes that modulated ω-3 polyunsaturated fatty acid dehydrogenase and Δ-12 fatty acid desaturase which were lack in pigs, a mutiple loci targeting vector that carrying the FAT1-FAT2 gene was constructed. 
This vector used the internal transcribe spacer sequences（ITS）that between the rRNA genes of pig as the target loci. To 
improve the efficiency of targeted integration, the positive and negative screening system of NEO and TK was introduced into 
the vector for double selection. EGFP reporter gene and Cre/Loxp system were added to the vector to screen out the positive 
clones of homologous recombination cells. The targeting vector with the FAT1-FAT2 gene was transfected into porcine kidney 
cell PK15 by liposome.【Result】RT-PCR results showed that FAT1-FAT2 gene expressed in porcine kidney cell PK15. The 
GC-MS results showed that the contents of ω-6 PUFAs and ω-3PUFAs in control group PN1（cells that transfected with PN1 
plasmid）, treatment group PF1（cells that transfected with FAT1 gene）, treatment group PF1F2（cells that transfected with 
FAT1-FAT2 gene）were 7.9%, 7.03%, 3.92%, 5.64%, 7.01% and 9.43%, and the results showed significant differences. The 
ω-6/ω-3 ratio decreased significantly from 1.4 in the control group to 0.42-1 in the experimental group.【Conclusion】FAT1-
FAT2 mRNA can be expressed and produced by transfecting pig kidney cell PK15 with FAT1-FAT2 double gene by constructing 
target vector, and the content of ω-3 PUFAs in transfected cells can be significantly increased, which lay a foundation for the 
subsequent production of transfected pig with FAT1-FAT2 double gene.]]></description>
<pubDate>2021/9/24 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[林　纯，汤　飞，李紫聪]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>林　纯，汤　飞，李紫聪</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202108014&flag=1]]></guid><cfi:id>85</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effects of Morphological Traits on Body Weight in the F5 Generation of Cyprinus carpio rubrofuscus Population]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202108015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was carried out to explore the effects of the morphological traits on body weight for 
Cyprinus carpio rubrofuscus F5 generation cultured in paddy fields.【Method】Nine growth traits including total length（X1）, 
body length（X2）, body height（X3）, head height（X4）, head length（X5）, caudal peduncle height（X6）, caudal 
peduncle length（X7）, body width（X8）and body weight（Y）of C. carpio rubrofuscus F5 generation cultured in paddy 
fields were measured. The path analysis was used to analyze the data.【Result】The body weight coefficient of variation of F5 C. carpio rubrofuscus cultured in paddy fields was 9.39%. Stepwise multiple regression analysis results showed that 
there were two morphological traits of body width（X8）and body length（X2）that significantly affected body weight. Direct 
path coefficient and indirect path coefficient of body width were 0.617 and 0.264, and direct path coefficient and indirect path 
coefficient of body length were 0.403 and 0.404. The discriminant formula was Y=-231.615+71.416X8+10.934X2（R2
=0.868）.
【Conclusion】The C. carpio rubrofuscus F5 generation was bred with the growth rate and body shape as breeding indicators. 
The body weight coefficient of variation of F5 generation was lower than that of control population, and the body shape of the 
selected offspring was more consistent. Body width and body length were the main morphological traits effecting body weight. 
The study results will provide basal data for further breeding of C. carpio rubrofuscus cultured in paddy fields.]]></description>
<pubDate>2021/9/24 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[樊佳佳，马冬梅，朱华平，黄樟翰，黄剑华，李辉安]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>樊佳佳，马冬梅，朱华平，黄樟翰，黄剑华，李辉安</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202108015&flag=1]]></guid><cfi:id>84</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effects of High Stocking Density on Rearing Environment and Growth Performance of Shan Partridge Ducks]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202107016&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Stocking density is one of the crucial factors influencing large-scale production of livestock 
and animal welfare. The study was carried out to explore the effects of different stocking densities on the rearing environment, 
body weight, blood endotoxin, tibia and pectoral/leg muscle growth of Shan Partridge ducks.【Method】A total of 180 
healthy 56-day-old female Shan Partridge ducks were divided into three groups with different densities: low density group（n 
= 30）, medium density group（n = 60）and high density group（n = 90）for land and water rearing. On the 0, 20 and 40 
days of the test, the number of bacteria, endotoxin in rearing water, body weight and blood endotoxin were measured, and the 
tibia and pectoral/leg musclewere studied by histomorphological and gene expression analysis.【Result】Under high stocking densities, the number of Escherichia coli and total bacteria in rearing water increased by at least 5 times（P < 0.05）, endotoxin 
in water and duck blood increased by more than 2 times（P < 0.05）, body weight decreased by about 0.1 kg on average（P < 
0.01）. In addition, high stocking densities decreased the thickness of bone and medulla substances of tibia by 100-200 μm （P < 0.05）, increased density and decreased diameter in pectoral muscle myofibers significantly, decreased density and 
increased diameter in leg muscle myofibers significantly, and increased expression of inflammatory cytokine IL-6 significantly 
and had significant effects on key muscle growth genes（Myod, Myog and Myh1）in pectoral/l eg muscles of Shan Partridge 
ducks.【Conclusion】Stocking density of 2.5-5 ducks/m2
 deceased the number of bacteria and endotoxin content in the rearing 
environment and promoted the pectoral/leg muscle and tibia development of Shan Partridge ducks, providing references for 
improving the growth performance of Shan Partridge ducks by optimizing the stocking density.]]></description>
<pubDate>2021/8/26 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[付梦思，王金辉，吴伟庆，黎颂超，江丹莉，黄运茂，田允波，张续勐]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>付梦思，王金辉，吴伟庆，黎颂超，江丹莉，黄运茂，田允波，张续勐</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202107016&flag=1]]></guid><cfi:id>83</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Isolation and Identification of Marine Bacillus and Determination of Its Digestive Enzyme Metabolites]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202107017&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study is aimed at isolating and identifying Bacillus which can produce digestive enzyme 
metabolites from marine organisms and environment, and providing probiotics for the development of its microecologics.
【Method】Samples were collected from the coastal waters of Leizhou Peninsula, and the isolated strains were obtained 
by high temperature treatment, enrichment culture, isolation and cultivation method. The isolated strains were identified 
by their colony morphology, cell structure, staining characteristics, biochemical test and 16S rRNA gene sequencing. The 
determination of protease, amylase and lipase activity in the metabolites of the isolated strains was detected by Folin phenol 
colorimetry, starch iodine colorimetry and olive oil emulsification method, respectively.【Result】Forty three suspected 
Bacillus strains are identified from 22 samples. Eight strains of them with the strongest comprehensive activities of three 
digestive enzymes are screened out, and identified through biochemical test and 16S rRNA gene sequencing as three 
strains of Bacillus cereus ,three strains of Bacillus siamensis, one strain of Bacillus tequilensis, and one strain of Bacillus 
xylanilyticus. Among them, Bacillus xylolylysine BC1 produces the highest lipase activity（52.13 U/mL）, Bacillus cereus
BC3 produces the highest protease activity（4.78 U/mL）and Bacillus Siamese BC6 produces the highest amylase activity （2.35 U/mL）.【Conclusion】Marine Bacillus producing digestive enzyme metabolites are screened out successfully.]]></description>
<pubDate>2021/8/26 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[伍楚妍，黄晓冰，刘少君，李　洋，谢为天，徐春厚]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>伍楚妍，黄晓冰，刘少君，李　洋，谢为天，徐春厚</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202107017&flag=1]]></guid><cfi:id>82</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[cDNA Cloning, Distribution and Purification of Chitinases from 
Gastrointestinal Tract of GIFT Tilapia（Oreochromis niloticus）]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202106015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Chitinases are the important hydrolytic enzymes, which can degrade the shells of shrimp and 
crab containing chitin in fish food by hydrolyzing β-1,4- glycosidic bonds. In this study, we tried to under the expression 
profile of chitinases in different tissues of tilapia and the characteristics of chitinase extracted from the stomach of tilapia. 【Method】Three chitinases, named tChit1a, tChi3 and tChit, were cloned from the stomach and intestines of GIFT tilapia. 
ORFs of these chitinases were analyzed by a series of bioinformatics software and their tissue distribution were detected by 
Real-time PCR. Next, the chitinases from stomach of tilapia were purified by affinity chromatography and the chitinase activity 
was detected by the 4-MU method.【Result】The genes tChit1a, tChi3 and tChit encoded 453, 453 and 473 amino acids, 
respectively. Homology alignment results showed that the homology between the deduced amino acid sequences of tChit1a 
and tChi3 was 83.66%, while tChit showed lower homology to tChit1a and tChi3, with 49.89% and 50.11%, respectively. The 
phylogenetic analysis indicated that tChit1a, tChi3 and tChit were classified into fish chitinases-3（FCase-3）rather than acid 
fish chitinase-1/2（AFCase-1/2）. The result of tissue distribution showed that tChit1a, tChi3 and tChit mainly expressed in 
the gastrointestinal tract of tilapia, with the highest mRNA expression in the midgut, foregut and midgut hind respectively. The 
purified chitinases from stomach of tilapia showed that there were two obvious bands with the size of about 40 ku, but no high 
homologous bands（Chitinase-1 of tilapia）were detected by polyclonal antibody of the Chitinase-1 of Epinephelus obliquus. 
The activities of hydrolyzed 4MU-（GlcNAc）2
 and 4MU-（GlcNAc）3
 were 1.73 and 4.89 U/g respectively at the optimum 
pH 5.【Conclusion】The low expression and activity of chitinases extracted from stomach of tilapia suggest that tilapia may 
have little interest in ingesting chitinous substances]]></description>
<pubDate>2021/7/21 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[黄金凤，李　波，白　莹，冯礼燊，李文笙，孙彩云]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>黄金凤，李　波，白　莹，冯礼燊，李文笙，孙彩云</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202106015&flag=1]]></guid><cfi:id>81</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Research Progress of Gene Recombination Technology in Genetic Engineering Vaccine of Porcine Pseudorabies Virus]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202105016&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[Porcine pseudorabies is an acute and highly contagious disease caused by pseudorabies virus (PRV) infection, which can cause disease in pigs of different ages and serious economic losses to the pig industry. One of the main measures to prevent, control and even eradicate porcine pseudorabies is vaccination. It is one of the effective methods to reduce the virulence of PRV or improve the immune protection effect of virus by transforming the key virulence genes of PRV, knocking out the virulence genes or inserting the immune enhancement genes through gene recombination technology, 
and it is also an important means to develop a safe and efficient genetic engineering vaccine of PRV. The development of porcine PRV genetic engineering vaccine was reviewed from the application of different gene recombination technologies, such as homologous recombination, Cre/loxP site-specific recombination, bacterial artificial chromosome and CRISPR gene editing, with a view to provide references for further study of PRV recombinant vaccine.]]></description>
<pubDate>2021/7/4 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[柯海意，王　帅，徐民生，蔡汝健，勾红潮，臧莹安，李春玲，简运华]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>柯海意，王　帅，徐民生，蔡汝健，勾红潮，臧莹安，李春玲，简运华</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202105016&flag=1]]></guid><cfi:id>80</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Preliminary Study on Intracellular Synthesis and Translocation of Rabies Virus M Protein]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202104016&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Rabies virus (RABV) is a highly neurotropic virus, which composes of five structural proteins, 
such as nucleoprotein (N), phosphoprotein (P), matrix protein (M), glycoprotein (G) and RNA-dependent RNA polymerase (L). 
The RABV genomic RNA, combining with N, P and L proteins, constitute the ribonucleoprotein complex (RNP). M proteins 
plays a particular role in regulating the synthesis and assembly of the structural proteins of RABV. However, it is not clear 
where the intracellular synthesis and translocation sites of M proteins are and whether there are differences in M proteins in 
different cell lines among RABV strains. The co-localizations of M protein with ER and Golgi, M protein with G protein and 
M protein with RNP in N2
A and BHK cells were studied to determine the intracellular synthesis pathway and translocation 
of RABV M proteins.【Method】TCID50 of different RABV strains (CVS-11, SRV-9 and PB4) in N2
A or BHK cells were determined. Co-localization of M protein with ER and Golgi as well as with G protein and RNP (represented by N protein) in ER 
and Golgi apparatuses were performed by Immunofluorescent.【Result】The titers in the different RABV strains had certain 
differences. All the M proteins in different RABV strains were co-localized with ER and Golgi. M protein also co-localized with 
G protein as well as with N protein; however, the co-localization of M protein and G protein was mainly in ER and Golgi, while 
that of M protein and N protein in cytoplasm.【Conclusion】The synthesis and processing of the RABV M proteins were carried 
out through ER-Golgi pathways. The study results confirmed the synthesis and translocation cellular sites of RABV M proteins, 
and enriched the data for assembly and budding process of RABV virions.]]></description>
<pubDate>2021/5/27 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[赵维荣，赵铭昕，徐　婧，张丹玮，郭艺迪，关振宏，段　铭，张茂林]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>赵维荣，赵铭昕，徐　婧，张丹玮，郭艺迪，关振宏，段　铭，张茂林</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202104016&flag=1]]></guid><cfi:id>79</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effects of Dietary Mulberry (Morus alba L.) Leaf Powder on Growth Performance, Meat Quality and Flavor of Grass Carp (Ctenopharyngodon idella)]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202104017&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The present study aims to evaluate the effects of different proportions of dietary mulberry 
leaf powder (MLP) on the growth performance, meat quality and flavor of grass carp in order to provide a theoretical basis for the application of MLP in aquaculture. 【Method】Five isonitrogenous (including 25.55% of protein and 3.25% of 
isolipid) diets respectively supplemented with 0 (control), 5%, 10%, 15% and 20% MLP were prepared. A total of 1 500 grass 
carp (initial body weight = 1 112.06±83.19 g) were randomly assigned to five dietary treatment groups and fed for 8 weeks.
【Result】Compared with the control group, the grass carp supplemented with 10% MLP in the diets had significantly higher 
values of the viscera somatic indexes. However, the supplementation of all proportions of MLP showed no significant effect 
on the final body weight, weight gain rate, specific growth rate, feed conversion ratio, survival rate, hepatopancreas somatic 
index and condition factor of grass carp. Supplementation of 5% MLP in the diets could significantly improve the hardness, 
gumminess, chewiness and resilience of the muscles, but had no significant effect on the springiness, cohesiveness and shear 
force of the muscles. Dietary supplementation of 10%, 15% and 20% MLP significantly reduced the cohesiveness of grass carp 
muscle, but showed no significant effect on the hardness, springiness, gumminess, chewiness and shear force of the muscles. In 
addition, both the compositions and contents of nutrients and flavor compounds in the muscles of experimental grass carp were 
analyzed. The results showed that the supplementation of 10% MLP in the diets significantly increased the contents of proteins, 
fat, delicious amino acids, flavor amino acids, essential amino acids, semi-essential amino acids, total amino acids, inosinic 
acid, unsaturated fatty acids and monounsaturated fatty acids, while significantly reduced the content of polyunsaturated fatty 
acids in grass carp muscles. The contents of the above nutrients and flavor compounds were also partially increased in other 
MLP groups.【Conclusion】The supplementation of no more than 20% MLP in the diet does not significantly affect the growth 
performance of grass carp, but can obviously increase the contents of proteins, inosine monophosphate, flavor amino acids and 
unsaturated fatty acids in the muscles as well as improve the meat quality and flavor of grass carp.]]></description>
<pubDate>2021/5/27 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[周东来，廖森泰，黄　勇，李　鑫，梁庆禄，杨　琼，邝哲师]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>周东来，廖森泰，黄　勇，李　鑫，梁庆禄，杨　琼，邝哲师</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202104017&flag=1]]></guid><cfi:id>78</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Comparison Test of Different Sweet Sorghum and Sorghumbicolor×Sorghum sudanense Varieties in Western Guangdong]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202102016&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was conducted to screen out sweet sorghum and Sorghum bicolor×Sorghum 
sudanense varieties suitable for planting in western Guangdong.【Method】Taking the introduced sweet sorghum and 
Sorghum bicolor×Sorghum sudanense as test materials, the phenological period, agronomic characteristics and conventional 
nutritional components（crude protein（CP）, crude fiber（CF）, ether extract（EE））of 8 sweet sorghum varieties
（Niumowang, Hainiu, Baizhan, Dalishi, Desheng, Daka, Juejia, Lvjuren）and 5 Sorghum bicolor×Sorghum sudanense
varieties（Baobaisi, Biaobingyucao, Jiebao, Deli, Leshi）were analyzed. And the organic matter digestibility was calculated.
【Result】There were some differences in phenological period, plant height, stem diameter and stem-leaf ratio among 
different varieties. The hay yield of sweet sorghum was 7.13-18.22 t/hm2, and the contents of CP, CF and EE were 5.97%-
8.99%, 22.91%-33.01% and 1.67%-3.00%, respectively. The hay yield of Sorghum bicolor×Sorghum sudanense
was 5.63-19.99 t/hm2, and the contents of CP, CF and EE were 6.39%-7.04%, 25.30%-30.44% and 1.51%-2.40%, 
respectively. Among the 8 sweet sorghum varieties, Desheng had the highest hay yield, which was significantly higher than 
that of other varieties. Among the top 3 sweet sorghum varieties in hay yield, the CP content of Desheng was significantly higher than that of Lvjuren and Dalishi, however, the digestibility of organic matter was significantly lower than that of Dalishi. 
Baobaisi had the highest hay yield among the 5 Sorghum bicolor×Sorghum sudanense varieties, which was significantly higher 
than that of other varieties. Baobaisi and Leshi were the top two Sorghum bicolor×Sorghum sudanense varieties in hay yield, 
but there was no significant difference in CP content and organic matter digestibility between them【Conclusion】Desheng, 
Dalishi and Baobaisi have good production performance in western Guangdong Province, and they are suitable to be promoted 
and planted in this area.]]></description>
<pubDate>2021/3/8 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[徐　磊，刘　洋]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>徐　磊，刘　洋</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202102016&flag=1]]></guid><cfi:id>77</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Transfer of Sex Identification Model for Silkworm Cocoons Based on Near-infrared Spectrum Among Multiple Devices and Varieties]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202102017&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】In the promotion and application of sex identification equipment of silkworm cocoons, in order to 
reduce the difficulty and time of new varieties and new equipment to be re-modeled, the transfer of a sex identification model for 
silkworm cocoons based on near-infrared spectrum among multiple devices and varieties was studied.【Method】Firstly, two 
kinds of spectrometers（NirQuest512 spectrometer and SW2540 spectrometer）were used to collect the near-infrared diffuse 
transmission spectrum data of four silkworm varieties（9fu, 7xiang, 7xia and 932）, then the convolutional neural networks 
trained by source domain dataset were used as the source domain model, and the outputs of the middle layer were analyzed 
visually. Furthly, the source domain model was tuned finely according to different silkworm varieties and different collection 
devices, and the transferred model was constructed. Finally, the model prediction accuracy after transfer learning was compared 
with the algorithm of Convolutional Neural Networks（CNN）, Support Vector Machine（SVM）and Random Forests （RF）.
【Result】The results show that the CNN source domain model constructed with 1 700 samples of 9fu（NirQuest512 
spectrometer）has good sex resolution ability, and the accuracy of sex resolution is over 99%. The SVM and RF models were 
constructed with the outputs of middle layer of CNN source domain model as inputs, and the accuracy of sex discrimination is 
over 92% and 90%, respectively. Visual analysis shows that the convolutional layer can well extract the sex characteristics. 
For the target domain with less sample size of 100 7xiang（NirQuest512 spectrometer）, 77 7xia and 112 932（SW2540 
spectrometer）, when the proportion of the training set is 70%, by fine-tuning the source domain CNN model, the accuracy of 
the obtained target domain CNN model is 96.90%, 99.67% and 97.29% respectively, with the optimal effect; the accuracy of 
the independent SVM model is 92.49%, 94.25% and 93.65% respectively, with the effect slightly lower than that of CNN; the 
accuracy of the independent RF model is 80.93%, 80.17% and 81.47% respectively, with the effect worse than that of SVM; 
the accuracy of the independent CNN model is only 58.87%, 56.33% and 72.17% respectively, with the worst effect. Through 
modeling comparison of the number of different training sets for many times, it is also shown that, in the case of less data, the 
effect of CNN model after transfer learning is the best, followed by the traditional machine learning method, and that of the 
deep CNN model is the worst.【Conclusion】The above results show the mobility of the deep transfer learning model with 
different spectrometers or different species, which provides theoretical and practical basis for the rapid establishment of a sex 
classification model for silkworm cocoons with multiple spectrometers and various varieties of cocoons.]]></description>
<pubDate>2021/3/8 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[陈楚汉 1
，钟苏苑 2，钟杨生 3，王先燕 2，代　芬 1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>陈楚汉 1
，钟苏苑 2，钟杨生 3，王先燕 2，代　芬 1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202102017&flag=1]]></guid><cfi:id>76</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effect of Chlorella pyrenoidosa on Antioxidant and Intestinal Bacterial in Mice]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202102018&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The effects of Chlorella pyrenoidosa on antioxidant and intestinal bacterial in mice were 
analyzed. 【Method】Fifty sparse-fur（SPF）mice were randomly divided into normal group（equal volume of normal 
saline, CK1）, VE group（100 mg/kg, CK2）, and different doses of C. pyrenoidosa（100 mg/kg, 200 mg/kg, 300 mg/kg）, 
respectively．After 3 weeks, the total antioxidant capacity（T-AOC）and superoxide dismutase（SOD） activity in serum and 
liver homogenates of mice were measured. Moreover, the DNA in mouse feces were exacted, and community structure diversity 
of intestinal bacteria was analyzed by using high-throughput sequencing technology.【Result】It was found that the activity of 
T-AOC and SOD in serum and liver of mice treated with different doses of C. pyrenoidosa were significantly higher than that 
of normal group. High-throughput sequencing results showed that the structures of mice intestinal bacteria were dominated by 
Bacteroides and Firmicutes. Compared with normal group, there was no significantly difference in the Alpha diversity indexes
（Shannon, Simpson, Chao1 and Coverage）of intestinal bacteria treated with C. pyrenoidosa. The results of PCA analysis 
indicated that there was significant difference in intestinal bacteria of mice between treatment with C. pyrenoidosa and normal 
group. Compared with normal group, the abundance of Bacteroides and Lactobacillus significantly increased in mice treated with 
C. pyrenoidosa, while the abundance of Muribaculum significantly decreased.【Conclusion】C. pyrenoidosa could increase 
the antioxidant activity, regulate abundance of beneficial bacteria in intestinal tract, and improve intestinal environment of mice]]></description>
<pubDate>2021/3/8 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[田　力，邱丹缨，温扬敏]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>田　力，邱丹缨，温扬敏</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202102018&flag=1]]></guid><cfi:id>75</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Determination of seven Penicillins in Feed by High 
Performance Liquid Chromatograph]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202101016&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was aimed to establish a HPLC method for the determination of amoxicillin, 
ampicillin, penicillin G, penicillin V, oxacillin, cloxacillin, and dicloxicillin in feeds.【Method】Firstly, the samples of 
formula feed, concentrated feed and concentrate supplement were extracted with 20% acetonitrile solution, and the premixed 
feed samples were extracted by EDTA-Mcllvaine buffer solution and purified by HLB solid-phase extraction column, and 
then derivated by the reagents. Eventually, the samples were separated on a C18（150 mm×4.6 mm, i.d 5 μm）column by 
gradient elution with acetonitrile - phosphate buffer solution as mobile phase, detected by UV detecor at 325 nm, calibrated 
by matrix matching standard working solution and quantified by external standard method.【Result】There were good linear 
relationships among the seven kinds of penicillins of formula feed, concentrated feed and concentrate supplement in the 
concentration range of 0.25-10 μg/mL, and there were good relationships among the seven kinds of penicillins of premixed additive feed in the concentration range of 0.10-10μg/mL. The average recovery rates were in the range of 69.6%-103.7% with 
RSD value less than 15%. The limit of detection（LOD）of compound feed, concentrated feed and concentrate supplement 
was 2.5 mg/kg , and the limit of quantification（LOQ）was 5 mg/kg. The LOD of premixed additive feed was 1.0 mg/kg and the 
LOQ was 2.0 mg/kg.【Conclusion】The method was proved to be specific, rapid, accurate and reproducible for determination of 
seven penicillin antibiotics in daily feed .]]></description>
<pubDate>2021/1/26 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[DING Chenhong，HUANG Xiaomei，WANG Weili，WAN Kai，SU Qiuquan，LI Yafei]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>DING Chenhong，HUANG Xiaomei，WANG Weili，WAN Kai，SU Qiuquan，LI Yafei</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202101016&flag=1]]></guid><cfi:id>74</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Research Progress in Application of 
Nanomaterials in Fish Vaccine]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202101017&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[Vaccines, mainly based on inactivated bacteria, are gradually replacing the antibiotics in fish disease 
treatment. However, the side-effects of vaccine injections can’t be ignored even though they are widely used and effectively 
in treating different aquatic diseases. In contrast, oral vaccines and immersion vaccines might be the best ways while these 
processes usually require repeated vaccinations due to the lower intake efficiency of antigens in fish. Nanoparticle, a vaccine 
delivery system, has been considered as a powerful tool to improve and solve these problems. As a dispersion or solid particle 
with low toxicity, good biodegradability, specific physical properties（size, surface charge, or loading capacity）as well as 
easily-controlled characteristic in delivery, nanoparticle could improve the targeting of immune system and the stimulation of vaccinations. The application of these nano-delivery systems in fish is still in the early stage of development. Here we reviewed 
the application progress of nanoparticles in fish vaccine research, including polylactic acid-glycolic acid nanoparticles, chitosan 
nanoparticles, liposomes nanoparticles and carbon nanotubes. The hot spots and problems in future fish vaccine study were 
proposed, providing a new idea for the development of fish vaccine.]]></description>
<pubDate>2021/1/26 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[CHEN Xinjin1, WANG Zhongliang1, HUANG Yu1，FAN Bolin1，JIAN Jichang1, 2, WANG Bei1, 2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>CHEN Xinjin1, WANG Zhongliang1, HUANG Yu1，FAN Bolin1，JIAN Jichang1, 2, WANG Bei1, 2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202101017&flag=1]]></guid><cfi:id>73</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Encoding Protein Expression of S10 Gene of Tilapia Lake Virus and Preparation of Monoclonal Antibody]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202111014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was carried out to prepare the monoclonal antibodies（MAb）of S10-segmentcoded protein against Tilapia Lake Virus（TiLV）.【Method】A recombinant expression plasmid pET32a -S10 that could efficiently express the TiLV S10 gene segment was constructed and it was converted into BL21（DE3）competent cells, which were induced by IPTG expression and purified by nickel column to obtain recombinant S10 protein with high purity. Female BALB/c mice aged 6-8 weeks were immunized with purified S10 protein to fuse their spleen cells with SP2/0 cells to obtain hybridoma cells. Two hybridoma cell lines that could steadily secrete anti-S10 protein MAb were screened by finite dilution and ELISA method. The hybridoma cells were named 2C3 and 2E3 respectively, and their specificity, subtype and titer were analyzed.【Result】Western blot results show that both 2C3 and 2E3 can recognize S10 recombinant protein and TiLV. Indirect immunofluorescence assay（IFA）results show that 2C3 and 2E3 only react positively with TiB cells infected with TiLV. Subtype detection results show that 2C3 antibodies are IgG1/к and 2E3 antibodies are IgG2a/к. Indirect ELISA shows that the titers of the two MAbs are 1:12 800 and 1:51 200, respectively.【Conclusion】The anti-TiLV-S10 protein MAb is specific and highly effective, which can provide materials and support for the development of TiLV vaccine, the establishment of immunological methods and the study of S10 protein function.]]></description>
<pubDate>2021/12/13 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[王雅慧，王英英，王　庆，李　波，李莹莹，尹纪元，杨　广，曾伟伟]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>王雅慧，王英英，王　庆，李　波，李莹莹，尹纪元，杨　广，曾伟伟</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202111014&flag=1]]></guid><cfi:id>72</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Establishment of Real-time PCR for Detection of Vibrio parahaemolyticus Causing Acute Hepatopancreas Necrosis Disease in Shrimps]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202111015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】A real-time PCR method was developed to detect Acute Hepatopancreas Necrosis Disease
（AHPND）caused by Vibrio parahaemolyticus（VpAHPND）for the rapid detection of such disease.【Method】A pair of 
specific primers and fluorogenic-labeled TaqMan probe were designed according to the conservative sequence of VpAHPND, 
and a real-time PCR method for the detection of VpAHPND was established by optimizing the reaction system and conditions. 
The standard curve was made with recombinant plasmids as standard products, and the specificity, sensitivity, repeatability 
and clinical application test were carried out.【Result】The method had a wide quantitative range from 2.3×101
 to 2.3×107
copies/μL and had linear relationship in its standard curve. The real-time PCR method had a high sensitivity with the 
detection limit as low as to 2.3 copies/μL for the purified recombinant plasmids of PMD-18T-pirB, and the entire detection 
could be completed within 1 h for a single sample. It also had a high specificity in detecting DNA of VpAHPND. The variation 
coefficients among cycle thresholds（Ct）of the repeatability test were 0.45%-0.69%. Comparing with the nested PCR recommended by OIE, the TaqMan probe real-time PCR had a same test rate of VpAHPND.【Conclusion】The TaqMan probe 
real-time PCR method was highly specific, sensitive and repeatable, which could be used for the early diagnosis of acute and 
latent infections of VpAHPND in shrimp samples.]]></description>
<pubDate>2021/12/13 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[陈平亚，张亮亮，吴少荣，黄纪徽，王绥家，吴山楠]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>陈平亚，张亮亮，吴少荣，黄纪徽，王绥家，吴山楠</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202111015&flag=1]]></guid><cfi:id>71</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Progress of the Application of CRISPR/Cas9 GeneEditing Technology in Pig Breeding]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202208011&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[China has a long history in the artificial domestication of wild boars. Many swine breeds with local 
characteristics have been bred in combination with local conditions, making China become the country with the richest 
swine resources in the world. Generally, most indigenous swine breeds in China present excellent meat quality traits such as 
 delicate tenderness and high intramuscular fat content, but also show slower growth rate, lower lean meat rate, and lower feed 
conversion ratio when compared to western swine breeds. Although cross-breeding through introducing western lean swine 
lineage could improve the growth and meat yield of indigenous swine breeds, there are adverse effects on meat quality traits. 
In addition, the introduction of western swine breeds has already brought a serious impact on local swine breeds, leading to 
a sharp decline in the number of many indigenous swine breeds in China, and even being threatened with extinction. The 
application of gene editing technology has brought many breakthroughs in the genetic improvement of indigenous swine 
breeds. Here, we review the developmental history of CRISPR/Cas9 gene editing technology, and progress of its application 
in the genetic improvement of swine, including reproduction, meat yield, disease resistance, and sex controlling. This review 
will facilitate to understand the special role of gene editing technology in domestic pig breeding.]]></description>
<pubDate>2022/10/9 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[XU Meina, ZHU Yizhou, LIN Siyuan, CHEN Yaosheng, HE Zuyong]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>XU Meina, ZHU Yizhou, LIN Siyuan, CHEN Yaosheng, HE Zuyong</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202208011&flag=1]]></guid><cfi:id>70</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Fitting Analysis of Growth Curves of Frizzle Chickenand Huaixiang Chicken Aged 0-16 Weeks]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202208012&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Frizzle chicken and Huaixiang chicken are excellent local breeds produced in the west 
of Guangdong. The early growth and development rules of the two local breeds of chickens are explored so as to provide 
a theoretical basis for the protection, development and utilization of their resources.【Method】By measuring the body 
weights of Frizzle chickens and Huaixiang chickens aged 0-16 weeks, Logistic, Gompertz and Bertalanffy models were 
used for fitting analysis of their growth.【Result】The fitting degree of the fitting curve of three models was above 0.979, 
and the fitting degree of Huaixiang chicken was generally better than that of Frizzle chicken; the fitting degree of Logistic 
model was poor, while the fitting degree of Bertalanffy model was the highest for Frizzle chicken and Huaixiang chicken, 
and it could better describe the actual growth situation of Huaixiang chicken. The ages of growth inflection point of Frizzle 
chicken and Huaixiang chicken fitted by Bertalanffy model were 11.4 weeks and 5.8 weeks respectively, and the weights of 
growth inflection point were 894.1 g and 498.7 g respectively. The growth curve equation of Frizzle chickens was as follows: 
Y=3017.521×〔1-0.726exp（-0.068t）〕3
, and the growth curve equation of Huaixiang chickens was: Y=1683.001× 〔1-0.787exp（-0.147t）〕3
.【Conclusion】Bertalanffy model is suitable for the growth curve fitting of Frizzle chickens and 
Huaixiang chickens, and the corresponding growth curve and key parameters are obtained through the analysis of the growth 
and development rules, which provides a certain scientific basis for the resource protection, breed selection and industrial 
development of the two local chicken breeds .]]></description>
<pubDate>2022/10/9 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[CAO Changren1
, ZHANG Danming2
, DU Bingwang2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>CAO Changren1
, ZHANG Danming2
, DU Bingwang2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202208012&flag=1]]></guid><cfi:id>69</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Research on the in vitro Antibacterial Activity andin vivo Anti-inflammatory and Analgesic Functions of Paederia scandens Extracted by Ethanol]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202208013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The antibacterial effect in vitro and the anti-inflammatory and analgesic effects in 
vivo of the effective active components of Paederia scanden were explored in order to provide theoretical support 
for the full utilization of P. scanden resources.【Method】The P. scanden was extracted with 40% ethanol solution, 
and the chemical components of the extract were preliminarily analyzed. The antimicrobial activities of the extract to 
Escherichia coli, Salmonella, Staphylococcus aureus and Bacillus cereus were detected by drug sensitivity test and double gradient dilution method to determine the minimum inhibitory concentration (MIC). The anti-inflammatory effect 
of P. scanden was explored by relieving xylene-induced ear swelling in mice and carrageenan-induced mice foot swelling. 
And the analgesic effect of acetic acid-induced writhing pain in mice was studied. 【Result】The ethanol extract of 
P. scanden contained many effective chemical components such as flavonoids and terpene lactones, which could inhibit the 
growth of B. cereus, with the inhibitory zone diameter of 8.55 mm and MIC of 500 mg/mL, but the antibacterial effect on E. coli, 
Salmonella and S. aureus was not obvious. Compared with the control group, the inhibitory rates of medium and high doses of P. 
scanden extract on mice ear swelling induced by xylene were 30.93% and 55.00%, and the inhibition rates of mice foot swelling 
induced by carrageenan were 49.74% and 58.26%, respectively, showing extremely significant anti-inflammatory effects, and 
they could effectively alleviate peripheral pain caused by glacial acetic acid in mice, delaying the time of the first writhing 
reaction in mice and reducing the frequency of writhing reaction within 15 minutes.【Conclusion】The ethanol extract of P. 
scanden contains rich medicinal activity. Although the antibacterial effect in vitro is weak, the anti-inflammatory and analgesic 
effects on tested mice are remarkable. Therefore, it can be used as one of the candidate Chinese herbal resources for animal feed 
additives.]]></description>
<pubDate>2022/10/9 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[YANG Ningyu，JIANG Jiali，LIANG Shuying，ZENG Lin，LIN Hongying,
CHEN Jinjun, KANG Danju]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>YANG Ningyu，JIANG Jiali，LIANG Shuying，ZENG Lin，LIN Hongying,
CHEN Jinjun, KANG Danju</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202208013&flag=1]]></guid><cfi:id>68</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effects of Feeding Frequency, Water Temperature and BreedingDensity on Growth Perfirmanc e, Intestinal Structure and HepaticGrowth-related Gene Expression Hepatic of Largemouth Bass]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202208014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】This experiment was conducted to research the effects of feeding frequency, water temperature 
and breeding density on growth performance, intestinal structure and hepatic GH and IGF-1 gene expression levels of 
largemouth bass in an indoor recirculating aquaculture system so as to determine the appropriate feeding frequency, water 
temperature and breeding density of largemouth bass.【Method】The experiment consisted of 8 treatments (Ⅰ - Ⅷ ). After 8 
weeks of breeding, the growth performance indicators such as specific growth rate (SGR), feeding rate (FR), feed coefficient (FCR) 
and survival rate, the intestinal villus height, villus width and muscle thickness, and the expression of hepatic growth factor GH
and IGF-1 genes of largemouth bass were evaluated.【Result】Only the main effects of breeding density on FR and feeding 
frequency on condition factor (CF) were significant, while the interaction effects had a significant effect on CF. The one-way 
ANOVA results showed that, the growth performance of treatment Ⅴ was significantly better than that of other treatments, and 
the SGR was significantly higher than that of treatments Ⅱ , Ⅳ , Ⅵ and Ⅷ , increasing by 10.16%, 10.61%, 9.27% and 8.4%, 
respectively; the FR was significantly higher than that of treatments Ⅰ , Ⅱ , Ⅳ and Ⅵ , increased by 10.94%, 27.02%, 26.45% 
and 17.38%, respectively; the FCR was significantly lower than that of treatments Ⅳ , Ⅷ and Ⅷ , decreasing by 7.87%, 8.89% 
and 8.89%, respectively. Compared with the treatmentⅤ, the FR of treatment I was significantly lower than that of treatmentⅤ, 
but there were no significant differences in SGR and FCR. The main effect and the interaction effect on intestinal villus height, 
villus width, muscle thickness and hepatic GH and IGF-1 gene expression levels were not significant. The intestinal villus 
height of treatment Ⅴ was significantly higher than that of treatments Ⅳ , Ⅵ and Ⅶ , villus width of treatments Ⅰ and Ⅴ was 
significantly higher than that of treatments Ⅲ , the intestinal muscle thickness of treatments Ⅰ and Ⅲ was thicker than that of 
treatment Ⅷ , and there was no significant difference in other treatments. The hepatic GH and IGF-1 gene expression levels of 
treatments Ⅰ and Ⅴ were significantly higher than those of other treatments.【Conclusion】The optimal feeding frequency 
for largemouth bass was 2-3 times/d, the water temperature was 25 ℃, and the breeding density was 25 fish per tank (150 L).]]></description>
<pubDate>2022/10/9 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[HUANG Wenqing1,2, HUANG Long3
, LAI Mingjian3
, LI Xiangni3
, CHEN Jiajia1,
LIU Fengkun1
, XU Zhixiong4
, HU Junru1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>HUANG Wenqing1,2, HUANG Long3
, LAI Mingjian3
, LI Xiangni3
, CHEN Jiajia1,
LIU Fengkun1
, XU Zhixiong4
, HU Junru1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202208014&flag=1]]></guid><cfi:id>67</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Research Progress in Genomics ofLumpy Skin Disease Virus]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202207013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[Lumpy skin disease (LSD) is an animal infectious disease caused by Lumpy Skin Disease Virus (LSDV) that 
produces nodular lesions in cattle, and is widespread in East, South and Southeast Asian countries, causing serious economic losses to the local cattle industry. Currently, LSD is managed as a second-class animal disease in China. Since 2019, more than 
ten provinces in China have reported LSD epidemics, whose situation is very serious. At present, the superior cell for LSDV 
isolation and proliferation is Madin-Darby bovine kidney (MDBK). As a member of the Goat Pox Virus genus of the Poxviridae 
family, LSDV has a virus particle diameter about 290 nm × 270 nm, with two existence forms. The viral genome is complex and 
huge, about 151 kb, and contains more than 150 genes. Currently, only approximately 40 LSDV strains containing the whole 
genome sequence information are stored in the GenBank database. In addition to field wild strains, recombinant strains of field 
wild strains and vaccine strains have also emerged in some parts of Asia in recent years, and the prevalent recombinant strains 
are pathogenic, which poses new challenges for development of vaccine and preparation of prevention and control strategies for 
LSD in the future. The study mainly summarizes the etiology of LSD and the progress in the genome-wide study of LSDV strains 
in different regions of Africa, Europe and Asia, with a view to providing references for the prevention and control of LSD and 
further researches.]]></description>
<pubDate>2022/9/5 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[ZHAI Qi, HUANG Minxia, LYU Dianhong, JIA Chunling, ZHOU Xiurong, WEN Xiaohui, LUO Shengjun]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>ZHAI Qi, HUANG Minxia, LYU Dianhong, JIA Chunling, ZHOU Xiurong, WEN Xiaohui, LUO Shengjun</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202207013&flag=1]]></guid><cfi:id>66</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Morphological Variation Analysis of Different Paphia textileGeographical Populations Along the Coast of Beibu Gulf]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202207014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was carried out to explore the morphological differences among three Paphia textile
populations, which was of great importance for germplasma resources conservation, geographical populations identification 
and artificial breeding of P. textile populations along the coast of Beibu Gulf.【Method】Based on 5 multivariate analysis 
methods (morphological characteristics analysis, variance analysis, principal component analysis, discriminant analysis 
and cluster analysis) were used to compare and analyze the morphological differences among three wild populations of P. 
textile in Beihai , Zhanjiang and Dongxing.【Result】The Beihai population in Guangxi had the most round shell, thicker 
shell and fuller soft part. The average shell height coefficient was 0.585, shell thickness coefficient was 0.033, shell weight 
coefficient was 0.388, and soft part weight coefficient was 0.134. The Dongxing population in Guangxi had thin and light shell with the flattest shell type. The average shell height coefficient was 0.571, shell thickness coefficient was 0.032, and 
shell weight coefficient was 0.135. The Zhanjiang population in Guangdong had thick shell with the lowest shell uplift degree. 
The shell width coefficient was 0.366, shell thickness coefficient was 0.035, and shell weight coefficient was 0.325. Three 
principal components were obtained by the principal component analysis method, the contribution rates of principal component 
1, principal component 2 and principal component 3 were 37.366 22.205% and 12.718%, respectively; and the cumulative 
contribution rate was 72.289%. Then, the discrimination functions of the 3 populations were set up, the identification accuracy 
was 94.00%-100.00% (P1
) and 92.11%-100.00% (P2
) and the total discriminate accuracy was 97.56%. The results of cluster 
analysis indicated that the morphological characteristics of Zhanjiang population were similar to those of Beihai population, 
but quite different from those of Dongxing population. In addition, Dongxing population had the highest degree of divergence.
【Conclusion】Compared with the Beihai population in Guangxi and the Zhanjiang population in Guangdong, the Dongxing 
population in Guangxi has the largest differences in morphology, and among individuals within the population, while the 
morphology of the Beihai population in Guangxi is more similar to that of the Zhanjiang population in Guangdong.]]></description>
<pubDate>2022/9/5 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[LAI Shengqi, YIN Cong, QIU Juwei, DIAO Hongshan, LI Zhimin, LIU Zhigang]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>LAI Shengqi, YIN Cong, QIU Juwei, DIAO Hongshan, LI Zhimin, LIU Zhigang</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202207014&flag=1]]></guid><cfi:id>65</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effects of Morphological Traits onBody Mass of Paphia textile]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202207015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was conducted to evaluate the effects of shell morphological traits on the body mass 
of wild Paphia textile and provide references for artificial breeding of P. textile. 【Method】Four traits including shell 
length (SL), shell height (SH), shell width (SW) and body mass (BM) of 121 P. textile randomly chosen from Beihai, Guangxi 
Province were measured. The shell traits (SL, SH, SW) were used as independent variables and body (BM) was used as 
dependent variable for correlation, path, determination coefficients and multiple regression analysis.【Result】The maximal 
coefficient of variance is in body mass, with extremely significant differences in correlation coefficients among the four 
quantitative traits of P. textile . The correlation coefficient between body mass and shell width is the maximal, with a value 
of 0.882, and the minimal correlation coefficient is between shell length and shell width, with a value of 0.596. The results 
of path analysis indicate that shell width has the greatest direct influence on body mass, and the path coefficient is 0.573. 
The indirect influence of shell height through shell width on body mass is the greatest, and the path coefficient is 0.423. The results of degree of determination analysis are consistent with those of the path analysis results. The optimal multiple regression 
equation of shell traits on body mass is established through stepwise multiple regression analysis: BM=-32.258+0.309SL+0.356
SH+1.456SW(R2
=0.874).【Conclusion】When it comes to artificial breeding of P. textile with body mass as breeding objective, 
shell width should be considered first and the cooperative selection of shell height should be strengthened at the same time.]]></description>
<pubDate>2022/9/5 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[ZHAI Ziqin, YU Dahui, BAI Lirong]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>ZHAI Ziqin, YU Dahui, BAI Lirong</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202207015&flag=1]]></guid><cfi:id>64</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Impact of Training Data Distribution on Plant Diseases 
Recognition Based on Deep Learning]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202206013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was carried out to improve the accuracy of the deep learning model through 
adjusting the distribution of training dataset of lab-condition and field-condition images, to reduce the dependence of 
plant diseases recognition models on field-condition data. 【Method】The plant diseases recognition model was optimized 
through adjusting the distribution of images of lab-conditions and field-conditions in training datasets. Deep learning models of plant diseases trained by three artificial neural networks of ResNeSt-50, VGG-16 and ResNet-50 were tested and 
compared. 【Result】In a training dataset composed of a certain number of plant disease images, it had an impact on the 
model accuracy through adjusting the distribution of images of different conditions. When the proportion of images of the fieldconditions reached 30%, the accuracy of the model was improved by more than 18%. Through adding field-conditions images at 
a number ratio of 30% into a training dataset composed of 100% lab-condition images, the accuracy of the model was improved 
by more than 17%. Through adding lab-conditions images into a training dataset composed of 100% field-condition images, 
the accuracy of the model was improved with the increasing number of images, and the improved ranges were between 2% and 
4%.【Conclusion】This method is suitable for the rapid establishment of high-accuracy plant diseases recognition models in 
the complex agricultural environment. It could reduce the dependence of plant recognition models on field-condition images, 
shorten the field data collection cycle at the beginning of model establishment and reduce the cost of field-condition images 
collection. It promotes a more effective application of artificial intelligence in unmanned farms and smart agriculture.]]></description>
<pubDate>2022/8/9 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[WANG Hongle, WANG Xinglin, LI Wenbo, YE Quanzhou, LIN Yonghai, XIE Hui, DENG Lie]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>WANG Hongle, WANG Xinglin, LI Wenbo, YE Quanzhou, LIN Yonghai, XIE Hui, DENG Lie</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202206013&flag=1]]></guid><cfi:id>63</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Isolation, Identificat ion and Biological Characteristics
of A Strain of Purpureocillium lilacinum]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202206014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was conducted to investigate the growth, protease and chitinase activities 
of a strain of Purpureocillium lilacinum isolated from the stroma of Aschersonia aleyrodis on citrus under different 
culture conditions, with a view to providing a theoretical basis for the use of P. lilacinum as a biocontrol agent.
【Method】The strains of the stroma of A. aleyrodis from Hongjiang orange leaves were isolated and purified by tissue 
isolation, identified by a combination of morphological characteristics and molecular methods, and their protease and 
chitinase activities were determined by the Folin-phenol and DNS method .【Result】The strain ZJPL1812 was identified as 
P. lilacinum by morphological observation and ITS sequence analysis. The best culture temperature for the strain was 30℃ , 
and the suitable pH value was 5.0-9.0. The best mycelial growth was achieved when ammonium dihydrogen phosphate and 
maltose were used as the nitrogen and carbon sources; the highest protease activity was produced when peptone and sucrose 
were used as the nitrogen and carbon sources; and the highest chitinase activity was achieved when ammonium nitrate 
and soluble starch were used as the nitrogen and carbon sources.【Conclusion】The strain can use many different kinds 
of nitrogen and carbon sources, with a wide range of acid-base adaptability and strong environmental adaptability. And it 
produces high protease and chitinase activities, indicating that it has some potential in biological control.]]></description>
<pubDate>2022/8/9 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[KE Xinru, LIU Dengyan, TAN Jianbin, SU Qi, LI Ya]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>KE Xinru, LIU Dengyan, TAN Jianbin, SU Qi, LI Ya</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202206014&flag=1]]></guid><cfi:id>62</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Applications of Base Editing Technology
in Modifications of Pig Genes]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202206015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[Pigs are highly valued in both agriculture and medicine. We can quickly improve the economic properties of 
pigs and develop human disease models via gene modification technologies. CRISPR/Cas9 gene editing technology, with the 
advantages of simple operation, high efficiency and low cost, is widely applied in modifying pig genes. However, CRISPR/
Cas9, due to its proneness to cause problems including structural instability of the genomes, large-scale chromosomal 
rearrangement and gene off-target, could pose potential risks to biological safety. In recent years, the base editing technology based on CRISPR/Cas9 can achieve the directional conversion of a single base without causing DNA double-strand breaks and 
insertion or loss (Indels), which is more precise and safer for gene editing. With the continuous development and improvement 
of base editing tools, it is not only able to produce base C → T (A → G) mutations, but also to produce simultaneous mutations 
of two bases (C and A) and base C → G, increasing the scope of application. improved base editing tools can reduce or even 
eliminate collateral mutations while maintaining editing efficiency, no C → T (A → G) mutations and Indels, making base 
editing technology has more important potential applications in pig genetic modification. In order to provide references for 
the application of base editing in pigs, the study reviews the systemic mechanism of different base editing technologies, the 
application of base editing technologies in the construction of genetically modified pig models and the genetic improvement of 
pigs as well as the editing efficiency and off-target situation of base editing systems in pig fibroblasts.]]></description>
<pubDate>2022/8/9 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[PAN Jiasheng, HUANG Qiuyan, YANG Yecheng, YANG Shuaipeng, ZHU Xiangxing, TANG Dongsheng]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>PAN Jiasheng, HUANG Qiuyan, YANG Yecheng, YANG Shuaipeng, ZHU Xiangxing, TANG Dongsheng</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202206015&flag=1]]></guid><cfi:id>61</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Comparative Genomics Analysis ofProtease Genes in Bacillus sp.]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202205013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study aims to explore the relationship between the protease-producing capacity of 
Bacillus and the protease genes carried by the bacteria and analyze the important enzyme genes that affect the proteaseproducing capacity of Bacillus.【Method】The protease-producing strain was screened by using the selective medium. 
The protease activity of the strains was determined by the Folin method. The protease genes carried on the chromosomes of 
the strains were obtained and classified by sequencing the whole genome of 15 strains of Bacillus. The transcription level of 
protease gene mRNA was measured by the real-time fluorescent quantitative PCR method. Through comparative genomics and Bacterial Genome-Wide Association Analysis (BGWAS), the reasons that affect the ability of bacteria to produce protease 
were explored. 【Result】According to the protease genes carried on the chromosomes of the strains, they could be divided 
into 7 groups of genotypes (G1-G7). Comparative genomics and BGWAS found that the number of protease genes carried on 
bacterial chromosomes was directly related to the protease production capacity, and the protease production capacity of strains 
lacking aprX and aprE was significantly reduced. The difference in the protease production capacity of Bacillus was found to 
be related to the expression level of aprX by further studying the mRNA level of the gene.【Conclusion】There is a correlation 
between the protease gene carried on the chromosome of the strain and the protease production capacity of the strain. The 
important enzyme gene that affects the protease production capacity of Bacillus is aprX.]]></description>
<pubDate>2022/7/11 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[JIA Zhongxin, ZHAO Jianan, JI Fang, WANG Xue, LI Gang, WANG Chengmin, QIN Jianhua]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>JIA Zhongxin, ZHAO Jianan, JI Fang, WANG Xue, LI Gang, WANG Chengmin, QIN Jianhua</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202205013&flag=1]]></guid><cfi:id>60</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Survey on Infection of Rabbit Coccidiosis and the SpeciesIdentification of Coccidia in Western Chongqing]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202205014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was carried out to understand the infection of rabbit coccidiosis and the 
species of coccidia, which provided a basis for the prevention and control of rabbit coccidiosis in western Chongqing.
【Method】A total of 1273 samples from 14 rabbit farms in 8 regions (including Rongchang, Yongchuan, Dazu etc.) in 
western Chongqing were examined by using the flotation technique with saturated salt solution and McMaster’s method, 
and the oocysts were collected to identify the species of coccidia【Result】There were coccidia infections in all the 14 
rabbit farms, in which the mean infection rate was 90.65%, and both the highest infection rates in Bishan and Jiangjin 
regions were 100%. The infection rate of rabbits less than 2 months of age was significantly higher than that of rabbits at 
other ages including 2-4 months, 4-9 months and more than 9 months, with an infection rate of 98.19%. The infection rate of 
rabbit coccidia in small-scale farms was significantly lower than that in large-scale farms, with an infection rate of 86.53%. 
The number of oocysts per gram (OPG) of the eight regions ranged from 9.65×103
 to 6.56×104
, and the highest OPG of rabbits at age of 2-4 months was 6.31×104
. According to a survey on the medicine use situation, the infection rate and OPG of 
farms without taking coccidial drugs were 92.31% and 4.42×104
, while the infection rate and OPG of farms taking coccidial 
drugs were 89.19% and was 2.75×104
. In total, 10 species of Eimeria were identified in 14 rabbit farms and the dominant 
species were E. perforans, E. magna, E. coecicola and E. media with detection rates of 25.20%, 23.38%, 11.96% and 10.02%, 
respectively.【Conclusion】Coccidia infection in rabbits is common in western Chongqing, most of which are mixed infections. 
Therefore, the prevention and control of rabbit coccidiosis should be strengthened in this area.]]></description>
<pubDate>2022/7/11 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[TAN Chun, JIA Hongguo, MEI Sipeng, WANG Bingnan, SUN Xue, LUO Shan, ZHOU Rongqiong]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>TAN Chun, JIA Hongguo, MEI Sipeng, WANG Bingnan, SUN Xue, LUO Shan, ZHOU Rongqiong</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202205014&flag=1]]></guid><cfi:id>59</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Construction of Growth Model for Mandarin Fish(Siniperca chuatsi) Guangqing No.1 fed withCompound Diet and cultivated in Pond]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202205015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was conducted to understand the growth and development law of Siniperca 
chuatsi Guangqing No.1 (hereinafter called mandarin fish) fed with compound diet and cultivated in pond so as to provide 
references for the breeding and large-scale culture of mandarin fish.【Method】The morphological parameters and 
growth performance of mandarin fish fed with compound diet at different month-ages in a whole cultivation period were measured. Three nonlinear models, Logistic, Gompertz and Bertalanffy, were used to construct the growth curves of both body 
mass and body length of the mandarin fish. 【Result】The maximum absolute body weight gain and absolute body length 
gain were found at 5-month-age fish, and 3-month-age fish, respectively. While the relative body weight gain and body 
length gain began to decrease from 4 months age. Morphological parameters, including condition factor (CF), viscerosomatic 
index (VSI) and mesenteric fat index (MFI) increased while hepatosomatic index (HIS) and intestine & stomach index (ISI) 
decreased as the fish month ages increased. The three models could simulate the growth curve of mandarin fish well, with 
a degree of fit R2 ≥ 0.97. Correlation analysis between the model predicted value and the measured value showed that 
the Logistic model was better than the other two models. The growth models for body mass and both length were as follows 
respectively, Wt
=1230.808/(1+39.666e-0.760t), R2
=0.989; Lt
=40.871/(1+3.076e-0.387t), R2
=0.990. The growth inflection point 
for body mass was at 4.843 months, corresponding to body mass and both length of 615.404 g and 20.436 cm, respectively.
【Conclusion】Logistic model is more suitable to describe the growth process of mandarin fish, and it can predict the growth 
data of different stages, which will provide theoretical basis for further breeding and large-scale culture of mandarin fish fed 
with compound diet.]]></description>
<pubDate>2022/7/11 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[WANG Fubao, CHENG Guangzhao, DONG Junjian, SUN Chengfei, WANG Miao, LU Maixin, YE Xing]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>WANG Fubao, CHENG Guangzhao, DONG Junjian, SUN Chengfei, WANG Miao, LU Maixin, YE Xing</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202205015&flag=1]]></guid><cfi:id>58</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Genetic Variation Analysis of ORF5 Genes of Porcine Reproductive and Respiratory Syndrome Virus in Guangdong Province]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202204012&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The molecular epidemiological dynamics of porcine reproductive and respiratory syndrome 
virus (PRRSV) in Guangdong Province in 2021 were monitored in order to provide references for the formulation of comprehensive prevention and control measures for PRRSV.【Method】521 tissue samples of diseased pigs were selected from 
37 large-scale farms that were tested positive as PRRSV in different regions of Guangdong Province for ORF5 gene sequencing, 
then DNAStar was used to analyze the nucleotide homology and deduced amino acid homology of the ORF5 gene, and Mega 
7.0 was used to construct ORF5-based phylogenetic tree.【Result】Positive rate of PRRSV was 24.4% in the collected 
samples, and the positive rate of pig farms was 45.9%. The ORF5 gene sequences of 17 strains of PRRSV from different pig 
farms were obtained by sequencing. Phylogenetic analysis showed that all the isolates belonged to the North America genotype. 
Among them, 5 strains belonged to the Lineage 8 (JXA1-like and CH-1a-like) subgroup, 9 strains belonged to the Lineage 
1 (NADC30-like and NADC34-like) subgroup, 3 strains belonged to the Lineage 3 (QYYZ-like) subgroup, and no strains 
of the Lineage 5 (VR2332-like) were detected. The nucleotide sequence homology of ORF5 gene of 17 strains was 81.3%-
99.3%, and the deduced amino acid sequence homology was 80.0%-98.3%. 【Conclusion】The predominate strains of PRRS 
in Guangdong Province in 2021 belonged to Lineage 1 (NADC30-like and NADC34-like) and Lineage 8 (JXA1-like and CH-
1a-like), accounting for 52.94% and 29.41%, respectively. Lineage 3 (QYYZ-like) was reduced to a secondary epidemic strain, 
accounting for 17.65%.]]></description>
<pubDate>2022/6/6 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[JIANG Zhiyong, CHU Pinpin, CHEN Tianbao, LI Chunling, CAI Rujian]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>JIANG Zhiyong, CHU Pinpin, CHEN Tianbao, LI Chunling, CAI Rujian</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202204012&flag=1]]></guid><cfi:id>57</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Research Progress in Classical Swine Fever Virus Detection and Classical Swine Fever Vaccines]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202204013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[Classical Swine Fever (CSF) is a highly infectious and fatal disease caused by Classical Swine Fever Virus 
(CSFV). It is recognized as one of the most serious viral diseases in pig industry. Since CSF was first reported in 1810, CSF 
has caused significant economic losses to the pig industry in the world and continues to threaten global pork production and 
human food security. CSF was listed as one of the most important notifiable infectious diseases by the World Organization 
for Animal Health (OIE), and it was listed as “Class I infectious diseases” in China. At present, CSF is clinically manifested 
complicated and changeably as acute form with high mortality or subacute type, chronic type, recessive type and continuous 
infection type with variable mortality, and often infected combined with various diseases. Vaccination is still one of the main 
means to prevent and control CSF in most countries, especially in developing countries. The development and application 
of various laboratory diagnostic methods and clinical detection techniques for CSFV antigens and antibodies play a very 
important role in the prevention and control of CSF. With the rapid development of biotechnology, more diagnostic techniques and new-type vaccines for CSFV have been developed and approved. CSFV antigen and antibody diagnostic techniques, and the 
development and prospects of live attenuated and new-type CSFV vaccines such as subunit and vector vaccines are reviewed in 
order to provide references for better prevention and control of CSF.]]></description>
<pubDate>2022/6/6 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[ZHANG Pu, CHEN Jiankai, LAI Yuehui, LIN Derui, LI Fukun, ZHOU Xiaomin, HOU Gaowei, QI Dongmei]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>ZHANG Pu, CHEN Jiankai, LAI Yuehui, LIN Derui, LI Fukun, ZHOU Xiaomin, HOU Gaowei, QI Dongmei</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202204013&flag=1]]></guid><cfi:id>56</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Identification and Enzyme Activity Comparison of Cellulase-producing Bacillus]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202204014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The research was carried out to screen out celllulase-producing strains from 14 strains of 
bacteria in order to provide a material basis for the development of new feed additives. 【Method】The cellulase-producing 
strains were screened out from 14 strains by Congo red staining. Then the colony morphologies of them were observed and 16S 
rDNA gene sequence homology was analyzed and identified. After comparing the ability of each strain to degrade cellulose by 
Congo red staining, the cellulase activity of each strain was detected by DNS method.【Result】Seven cellulase-producing 
strains were screened, including 4 strains of Bacillus licheniformis and 3 strains of Bacillus subtilis. Based on Congo red 
staining, it was preliminarily identified that the cellulose degradation ability of 7 Bacillus strains was showed as: LW006>LW
005>LW004>LW002>LW007>LW003>LW001. Among them, strain LW006 (B. subtilis) had the strongest ability to degrade 
cellulose, producing a clear circle with a diameter of 22.5 mm. The cellulase activities of 7 strains of Bacillus were determined 
by DNS method as follows: LW006>LW005>LW004>LW007>LW003>LW002>LW001. Among them, strain LW006 
(B. subtilis) showed the highest cellulase activity with 1.22(±0.07) U/mL (P<0.01). 【Conclusion】Strain LW006 is a relatively 
high cellulase-producing strain, which is expected to provide new strain resources for new feed additives.]]></description>
<pubDate>2022/6/6 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[JIANG Xuebin, CHENG Xuehong, MA Miaopeng, DENG Zhongqing, LI Jiahui]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>JIANG Xuebin, CHENG Xuehong, MA Miaopeng, DENG Zhongqing, LI Jiahui</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202204014&flag=1]]></guid><cfi:id>55</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effects of Compound Probiotics on Growth Performance, Muscle Quality and Aquaculture Water of Tilapia Under Zero-water Exchange Condition]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202204015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was conducted to explore the effects of compound probiotics on the growth 
performance and muscle quality of tilapia and aquaculture water environment under zero-water exchange condition, with 
a view to providing references for emission reduction as well as efficient and healthy aquaculture in fishery industry. 【Method】Taking tilapia as research object and regular water exchange aquaculture mode as control group, a full-cycle 
aquaculture test of tilapia was carried out by adding compound probiotics under zero-water exchange condition (experimental 
group), and a comparative analysis on growth performance, muscle quality of tilapia and main water quality, microbial 
community structure was conducted.【Result】Under zero-water exchange condition, the survival rate of tilapia of the 
experimental group was 83.3%, significantly higher than the control group; feed coefficient was 2.3, significantly lower than 
the control group; as for the muscle quality, crude protein content of the experiment group was 22.17%, significantly higher 
than the control group. Main water quality of the experimental group stayed stable through the full-cycle of aquaculture period, 
the water temperature was 26.5-30.5℃ , pH was 7.0-8.0, nitrite of water sample was 0.039 mg/L, significantly lower than the 
control group. The dominant bacteria was similar between experimental and control groups, however, the diversity index of the 
experimental group was higher than that of the control group.【Conclusion】Under zero-water exchange condition, adding 
compound probiotics in aquaculture water improved the survival rate of tilapia, reduced the feed conversion rate, and improved 
the muscle quality of tilapia. Meanwhile, the experimental group had strong improving effects on the water environment of 
aquaculture to some certain extent, and increased the diversity index of bacterial community. During the whole aquaculture 
production cycle, the zero-water exchange aquaculture of tilapia with high-activity compound probiotics had a good substitution 
effect for the conventional aquaculture model which relies on frequent water changes to release environmental pressure.]]></description>
<pubDate>2022/6/6 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[CHEN Liting, 
　WU Jianfeng, ZHAO Yubing, YAN Xin, XIAO Jun, LUO Yongju, XIANG Guide, LIANG Junneng]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>CHEN Liting, 
　WU Jianfeng, ZHAO Yubing, YAN Xin, XIAO Jun, LUO Yongju, XIANG Guide, LIANG Junneng</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202204015&flag=1]]></guid><cfi:id>54</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effect of Chicken Coccidia Vaccine and Anticoccidial DrugsShuttle Program on the Drug Resistance of Coccidia]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202203013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was carried out to investigate the effect of biological shuttle program using coccidia 
vaccines and anticoccidial drugs on the drug resistance of chicken coccidia in broiler farms.【Method】Huadu isolates of 
Eimeria tenella were isolated and identified from five chicken farms in Huadu District, and these farms have been using 
coccidial vaccine and anti-coccidial drug shuttle program. Yingde isolates of E. tenella were isolated from five chicken farms 
in Yinde City, and the five farms have been using only anti-coccidial drugs to control coccidiosis. The resistances of Huadu and 
Yingde isolates to 6 anticoccidial drugs were tested respectively by using indicators including anticoccidial index (ACI), rate 
of reduction in lesion score (RLS), percentage of optimum anticoccidial activity (POAA) and relative oocyst production (ROP).
【Result】The cecal tissue sections of chickens of the two challenged groups showed that the mucosal layer was extensively 
affected with loss of intestinal glands, inflammatory cell infiltration, and many coccidial oocysts were found in the mucosa 
and submucosa. The results of ACI showed that the Huadu isolates were resistant seriously to nicarbazine, clodipidine and 
maduramycin, and moderately resistant to other 3 drugs; and the Yingde isolates were moderately resistant to all 6 drugs. The 
results of POAA showed that the Huadu isolates were sensitive to the mixture of maduramycin and nicarbazine, but resistant 
to other drugs, while the Yingde isolates were sensitive to all 6 drugs. The results of RLS and ROP showed that the two mixed 
isolates were resistant to all 6 drugs. Based on comprehensive evaluation, the Huadu isolates were resistant moderately to 
mixture of maduramycin and nicarbazine, and resistant seriously to other five drugs; while the Yingde isolates were resistant 
moderately to all six drugs.【Conclusion】The field isolates of chicken coccidia were resistant to anticoccidial drugs at different 
levels with multi-drug resistances. Both isolates from Huadu District and Yingde City showed resistance to six drugs moderately 
or seriously, suggesting that biological shuttle program could not significantly restore sensitivity of E. tenella compared to 
program using anticoccidial drugs only.]]></description>
<pubDate>2022/5/7 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[WANG Qing, ZENG Xianjun, FAN Zhihong, HE Jiana, KANG Huahua, HE Shunian, CHEN Jie, PENG Xinyu]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>WANG Qing, ZENG Xianjun, FAN Zhihong, HE Jiana, KANG Huahua, HE Shunian, CHEN Jie, PENG Xinyu</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202203013&flag=1]]></guid><cfi:id>53</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[An Overview of Research on Culture History,Application and Culture Conditions of Fish Cell]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202203014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[Cell culture refers to the method that tissues are separated into single cells by enzyme, mechanical or 
chemical methods, and the growth and development of cells are simulated by the animal’s internal environment. Based on 
the potential applications of the fish model, various tools and techniques, including in vitro cell culture, have been developed. 
With the rise of biotechnology in the 20th century, in vitro cell culture has gradually been penetrated into various disciplines, 
such as immunology, pharmacology, toxicology, genetics and tissue regeneration and transplantation. In vitro cultured cells 
have the advantage of imitating the genetic homogeneity of host cells, and the reaction variability in vivo can be reduced to 
a certain extent in the experimental process, with low cost, good repeatability, and less moral constraints. Fish cell culture 
technology was developed in the 1960s and was first applied to virus isolation. Cells can be obtained from different organs 
and tissues. Although the research of fish cell culture technology is later than that of mammals, in the long run, the indepth study of fish cells has far-reaching significance and broad development prospects for both theoretical research and 
practical application. This review briefly discusses the history, application and technology of fish cell culture, which provides 
reference for future research on fish cell culture technology.]]></description>
<pubDate>2022/5/7 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[MAO Meiqin, LAN Zhenyu, LI Jin, GUO Xiyi, HUANG Fengping, LIAO Yuanlong , CAI Xiaohui]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>MAO Meiqin, LAN Zhenyu, LI Jin, GUO Xiyi, HUANG Fengping, LIAO Yuanlong , CAI Xiaohui</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202203014&flag=1]]></guid><cfi:id>52</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effect of Circular RNA hsa_circ_004389 onReplication of Influenza A Virus]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202202016&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Based on the preliminary transcriptome profiles of A549 cells responding to influenza A virus 
(IAV) infection, one of the most significantly up-regulated host circular RNA(circRNA), hsa_circ_004389, was selected to 
further validate its expression and explore its roles in IAV replication.【Method】First, real-time quantitative PCR (qPCR) 
was used to verify the expression levels of hsa_circ_ 004389 at different time courses and different multiplicity of infection 
(MOI) of IAV infection in A549 cells; Second, hsa_circ_ 004389 expression in A549 cells treated with poly: I∶C (polyinosinic 
acid ∶ polycytidylic acid) and LPS (Lipopolysaccharide) were detected, respectively; Third, A549 cells were treated with 
interferon beta (IFNβ) and JAK inhibitor, and the expression of hsa_circ_004389 was determined; Finally, after a transient 
overexpression and knockdown in A549 cells, IAV replication at mRNA and protein levels were determined.【Result】
There was a positive correlation between the expression of hsa_circ_004389 and IAV replication. JAK-STAT signaling path 
was involved in regulating hsa_circ_004389 expression, and hsa_circ_004389 had the ability to inhibit IAV replication. 【Conclusion】Hsa_circ_004389, the product of the host responding to IAV infection, is a potential host anti-IAV factor.]]></description>
<pubDate>2022/3/29 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[ZHANG Shu, YU Xiaohang, ZHANG Le, SU Ning, ZHAO Lili, DUAN Ming]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>ZHANG Shu, YU Xiaohang, ZHANG Le, SU Ning, ZHAO Lili, DUAN Ming</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202202016&flag=1]]></guid><cfi:id>51</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Cloning of IgD Gene in Swordtail Fish and Changes of Its Responses to Aeromonas hydrophila Vaccine Challenges]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202202017&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The swordtail fish (Xiphophorus helleri) was employed to clone the gene sequence of 
immunoglobulin D (IgD), and the tissue distribution and expression laws after vaccine immunization of the IgD in swordtail 
fish were explored.【Method】With the known EST libraries, the cDNA sequences of IgD gene from swordtail fish were cloned and analyzed, and the tissue distribution and the relative expression changes in head kidney, spleen and intestine 
after vaccination were detected by real-time PCR.【Result】The complete open reading frame (ORF) of swordtail fish was 
3 879 bp, which encoded 1 298 amino acids, predicting that the molecular weight was 145.5 ku. The IgD of swordtail fish was 
composed of variable region and constant region, and the constant region contained CH1-CH2-CH3-CH4-CH5-CH6-CH7 
and a transmembrane region (TM). The systematic evolution analysis of gene sequences showed that swordtail fish IgD shared 
similarities of 70% with mandarin fish (Siniperca chuatsi). IgD mRNA expression of swordtail fish was highly detected in head 
kidney, spleen and intestine. After being immunized with Aeromonas hydrophila vaccine, the expression of IgD in the head 
kidney and spleen was significantly down-regulated, and it was significantly up-regulated in the intestine.【Conclusion】IgD 
may play an important role in immune response and intestinal mucosal immunity of swordtail fish.]]></description>
<pubDate>2022/3/29 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[WANG Pianpian, KANG Huahua, LIU Zhenxing, WANG Fang, LIU Chun]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>WANG Pianpian, KANG Huahua, LIU Zhenxing, WANG Fang, LIU Chun</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202202017&flag=1]]></guid><cfi:id>50</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Identification of Proteins Interacting with PA-X from H7N9 Avian Influenza Virus by GST pull-down Combined with Mass Spectrometry]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202201014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】In order to explore the function of PA-X protein from H7N9 avian influenza virus (AIV), 
the host proteins interacted with PA-X were screened and identified by GST pull-down and mass spectrometry technology.
【Method】The recombinant expression vector pGEX-4T-PA-X was constructed and transformed into Escherichia coli
BL21 (DE3) cells to express the recombinant protein, and GST-PA-X fusion protein was purified with GST affinity resin. 
With GST pull-down technique, GST-PA-X and GST control protein were respectively incubated with total proteins from 
the A549 cell lysate in vitro, the protein complexes were separated by SDS-PAGE and then analyzed by mass spectrometry.
【Result】The mass spectrometry analysis demonstrated that 39 candidate proteins with a high credibility might interact 
with PA-X. Through the enrichment analysis of GO and KEGG pathway, it was found that these proteins were mainly 
involved in biological processes such as RNA metabolism and processing, mRNA translation and transport, peptide biosynthetic process, and posttranscriptional regulation of gene expression and so on. As one of the key candidate proteins, 
Hsp70-2 was further chosen to explore its interaction with PA-X. The immunofluorescent analysis demonstrated that Hsp70-2 
and PA-X co-localized in the cytoplasm of the A549 cell line. This further confirmed the interaction between cellular Hsp70-
2 and PA-X.【Conclusion】Thirty-nine candidate proteins were identified to interact with PA-X in A549 cells via the GST 
pull-down combined with mass spectrometry technology, and the colocalization between Hsp70-2 and PA-X was confirmed by 
the immunofluorescence test. These results provide a basis for a further research on the role of PA-X in the life cycle of AIV.]]></description>
<pubDate>2022/2/25 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[XU Guoshuang, JIANG Tongxiao, GUO Yidi, DUAN Ming, ZHANG Maolin, GUAN Zhenhong]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>XU Guoshuang, JIANG Tongxiao, GUO Yidi, DUAN Ming, ZHANG Maolin, GUAN Zhenhong</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202201014&flag=1]]></guid><cfi:id>49</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Metabolic Characteristics of Different Carbon Sources by Microorganisms in Sediment of High-density Tilapia Pond]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202201015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Microbial community in sediment plays an important role in maintaining the balance of 
pond ecosystem. The present research aimed to explore the characteristics and seasonal variation of carbon and nitrogen 
metabolism of microorganisms in sediment of high-density tilapia pond.【Method】Sediment samples were collected 
during the aquaculture season, and the contents of nitrate nitrogen, nitrite nitrogen, ammonia nitrogen and total nitrogen 
in sediment were analyzed. The average well color development (AWCD) of microbial metabolism of substrates containing 
both C and N, containing both C and P and containing C only was analyzed by Biolog-ECO plates. 【Result】The contents of total nitrogen and ammonia nitrogen in pond sediment in July and October were significantly higher than those 
in April. The microorganisms in the sediment could use substrates containing C only, including β-methyl-D-glucoside, 
pyruvic acid methyl ester, D-galacturonic acid, tween 80, D-mannitol and D-cellobiose, substrates containing C and N, 
including L-asparagine and N-acetyl-D-glucosamine (AWCD ＞ 0.5), and substrates containing C and P, including D,L-α-
glycerol phosphate and glucose-1-phosphate (AWCD ＜ 0.5). 2-hydroxy benzoic acid, itaconic acid and α-ketobutyric acid 
could not be utilized. In April, AWCD of substrates containing C and N and substrates containing C only was higher 
than those in July and October. AWCD of substrates containing C and N, and substrates containing C only was positively 
correlated with nitrate content in sediment.【Conclusion】Biolog-ECO plate technology could be used to analyze nitrogen 
use index of sediment microorganisms for evaluating nitrogen removal capacity of microbial community in sediment.]]></description>
<pubDate>2022/2/25 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[WANG Miao, YI Mengmeng, WANG Ruining, LU Maixin]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>WANG Miao, YI Mengmeng, WANG Ruining, LU Maixin</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202201015&flag=1]]></guid><cfi:id>48</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effects of Salinity, Temperature and Light on the Growth of Chaetoceros muelleri from the Coast of Guangxi]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202201016&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Combining with the seawater environmental conditions in Fangchenggang sea area along 
the coast of Guangxi, the main physical and chemical factors of the growth of Chaetoceros muelleri was explored and the 
optimal growth environment conditions were screened out in order to provide technical data for large-scale production.
【Method】The effects of salinity, temperature and light on the growth of C. muelleri were studied by single factor 
method. The experimental design was as follows: seven gradients of 0‰ , 5 ‰ , 10‰ , 20‰ , 30‰ , 35‰ and 40‰ were 
set for salinity test, four gradients of 25, 28, 31 and 34 ℃ were set for temperature test, and three gradients of 1 000, 
4 000 and 7 000 lx were set for the light intensity.【Result】C. muelleri could grow normally with the salinity of 5‰ - 40‰ , among which the salinity of 20‰ was the optimum growth condition. Under the condition of high temperature culture, 
the cell concentration of C. muelleri reached the maximum at 31 ℃. The lower the temperature, the slower the growth rate, the 
longer the stable period; the higher the temperature, the faster the growth rate and the shorter the stable period. 4 000 lx was 
the most suitable light intensity for the growth of C. muelleri. The higher the light intensity, the longer the stability period.
【Conclusion】The best growth conditions for C.muelleri in Fangchenggang sea area along the coast of Guangxi were: 20 ‰ 
salinity, 31 ℃ temperature and 4 000 lx light intensity, Under such conditions, both the growth rate and algal cell density 
reached the highest values. Excessive or low salinity, temperature and light could inhibit the growth of C. muelleri.]]></description>
<pubDate>2022/2/25 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[ZHU Xien, LIN Yuanyou, XIANG Guide, XIONG Jianhua, CHEN Tiancong, CHEN Xiaohan, ZHANG Bin]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>ZHU Xien, LIN Yuanyou, XIANG Guide, XIONG Jianhua, CHEN Tiancong, CHEN Xiaohan, ZHANG Bin</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202201016&flag=1]]></guid><cfi:id>47</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Genetic Evolution Analysis of Porcine Circovirus Type 2 in Yunnan Province]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202212012&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was carried out to investigate the prevalence of porcine circovirus Type 2 (PCV2) in pigs and cattle in Yunnan Province, and to identify the genetic variation and molecular evolution trend of PCV2 strains in Yunnan Province.【Method】PCR method was used to detect the etiology of PCV2 in 783 blood and manure samples of pigs and cattle from 11 regions of Yunnan Province. The whole genome amplification, cloning and sequencing of 6 PCV2 positive samples were carried out. The whole gene and ORF2 amino acid sequences were compared and analyzed by DNAStar software, and the genetic evolution tree was drawn by MEGA software.【Result】The positive rate of PCV2 of detected samples was 48.73%. A total of 6 strain PCV2 genome sequences were measured, of which 5 strain were 1 767 bp and 1 strain was 1 768 bp. The genetic development analysis showed that there were 2 strains of PCV2a subtype, 2 strains of PCV2b subtype and 2 strains of PCV2d subtype. The homology of 6 strains PCV2 was 94.6%-99.9%, and the homology with 20 national reference strains in GenBank database was 91.4 %-99.8 %. The ORF2 of 6 strains PCV2 had their typical amino acid sites. The amino acid site of PCV2a strain was mutated to be consistent with PCV2 (b/d) at the 80th (V80L) amino acid site, and the amino acid site of PCV2b strain was mutated to be consistent with PCV2d at the 59th (R59K), 63rd (K63R), 190th (A190T) and other amino acid sites.【Conclusion】PCV2 commonly exists in pigs in Yunnan, and the three gene subtypes of PCV2a, PCV2b and PCV2d coexist, and PCV2 (a/b) has a tendency to mutate to PCV2d. Holstein cows have not been infected with PCV2.]]></description>
<pubDate>2023/2/6 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[HE Shuai1, ZHANG Yuzhu1, LYU Nianci2, ZHANG Yifang3, CHAI Jun3]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>HE Shuai1, ZHANG Yuzhu1, LYU Nianci2, ZHANG Yifang3, CHAI Jun3</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202212012&flag=1]]></guid><cfi:id>46</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Analysis of the Trigeminal Comb Traits in White-ear Yellow Chicken]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202212013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was carried out to find candidate single nucleotide polymorphisms (SNP) loci associated with the trigeminal comb traits in white-ear yellow chickens.【Method】Sixty white-ear yellow chickens were used for crossbreeding experiment and 120 white-ear yellow chickens were used for fertility rate experiment. The blood samples of eighty-two white-ear yellow chickens were collected for DNA extraction, and then genotype classification was conducted by using Illumina chicken 60 k microarrays. After using PLINK 1.90 for quality control of the genotyping results, GEMMA software was used to perform genome-wide association analysis (GWAS) on SNP and traits to find SNP loci significantly associated with trigeminal comb traits in white-ear yellow chickens.【Result】The trigeminal comb of white-ear yellow chicken showed autosomal inheritance and the egg fertility rate was not significantly different from that of the white-ear yellow chicken with single comb. 55 023 valid SNPs were obtained from the population of 82 white-ear yellow chickens and there was no significant population stratification within the population. Ten SNP loci associated with trigeminal 
comb were located on chromosomes 1, 2, 3, 4, 5, 12 and 14 of white-ear yellow chickens, adjacent to or located on SPRY2, NDFIP2, ITGA9, EMC2, TMEM17, EHBP1, SCAF8, TIAM2, HTT, STON2, PRKCD, RBBP6 ,TNRC6A and MAPK8IP3 genes.【Conclusion】Based on the GWAS analysis, it is found that 10 SNPs loci may be associated with the trigeminal comb traits in white-ear yellow chickens. These results will provide candidate molecular markers for the breeding of white-ear yellow chickens and new ideas for marker-assisted selection of local chickens.]]></description>
<pubDate>2023/2/6 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[ZHANG Wenwu1, QIU Fengfang1,XU Jiguo2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>ZHANG Wenwu1, QIU Fengfang1,XU Jiguo2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202212013&flag=1]]></guid><cfi:id>45</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Research Progress in Nutritional Requirements and Feed for Yellow-feathered Broiler Breeders]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202212014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[In order to promote the efficient and healthy development of yellow-feathered broiler breeders, it is very important to solve the existing problems such as dietary waste, environmental pollution and low reproductive efficiency. The paper summarized the relevant domestic and foreign literature on nutritional requirements and application of feeds of yellow-feathered broiler breeders. It summed up studies about requirements of metabolic energy, crude protein, main essential amino acids (methionine, lysine, threonine, tryptophan, arginine and isoleucine), vitamins (VA, VD and VE), and mineral elements (Ca, P, Fe, Cu, Zn, Mn and Se), feed resource evaluation and utilization, application of bioactive additives, probiotics, plant extracts and Chinese herbal preparations, anti-stress agents in breeders of yellow-feathered broider, and analyzed the trend of future researches. On the whole, in recent years, there have been more and more researches on nutritional requirements and application of feed additives in yellow-feathered broiler breeders, but mainly concentrated in the egg-laying period. The highlight in the researches on nutritional requirements in recent years was correlation analysis between metabolic energy requirements per day and metabolic body weight, average daily gain, daily egg production. The prediction models of dietary nitrogen-corrected metabolic energy requirements for three types of breeder hens of yellow-feathered broilers were established with metabolic weight, average daily gain and egg production as variables. At present, there are few reports on nutrition and feed restriction technology in the brooding period and growing period of yellow-feathered broiler breeders. Key techniques for feed resource evaluation and safe & efficient utilization and reproductive performance improvement of breeder hens still need to be broken through.]]></description>
<pubDate>2023/2/6 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[ZHANG Sheng, JIANG Shouqun]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>ZHANG Sheng, JIANG Shouqun</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202212014&flag=1]]></guid><cfi:id>44</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effect of Phaeodactylum tricornutum and Chlorella Powder on the Growth of Juvenile Haliotis discus hannai]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202212015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study aimed to investigate the effect of adding microalgae on the growth of juvenile Haliotis discus hannai, and explore the feasibility of Phaeodactylum tricornutum and Chlorella as feed additives for juvenile abalone.【Method】1%, 3% and 5% of P. tricornutum and Chlorella powder were added to the feed (marked as PT1, PT3, PT5, CH1, CH3, CH5, respectively), and compare the growth parameters, total antioxidant capacity (T-AOC), superoxide dismutase (SOD), α-amylase (AMS) and cellulase (CL) activity of juvenile abalone under various treatments, the number of bacteria and vibrio in the visceral sac were compared.【Result】The addition of P. tricornutum and Chlorella powder to the feed could significantly increase the shell length , and significantly reduce the mortality of juvenile abalone . The shell length of PT5 was the highest (16.38±2.49 mm), and the mortality of CH1 treatment was the lowest (2.007%±0.67%). The addition of P. tricornutum to the feed significantly increased the body weight and weight gain rate, and decreased the feed coefficient. After 40 days of breeding, the SOD activity of PT5 treatment (12.9±0.75 U/mg) and CH5 treatment (12.99±0.42 U/mg) was significantly higher than that of the control group . The T-AOC activity of PT3 and PT5 (1.15±0.03 U/mg,1.19±0.06 U/mg) was significantly higher than that of the control group. The AMS activity of PT5 (4.92±0.74 U/dL) was significantly higher than that of the control group, and there was no significant difference in CL activity among different groups. After 40 days of breeding, the number of bacteria and vibrio in the visceral sac of PT5 and CH5 was lower than that of the control group .【Conclusion】The effect of adding P. tricornutum on the growth of juvenile abalone was better than that of Chlorella, and the effect of adding 5% P. tricornutum was the most significant.]]></description>
<pubDate>2023/2/6 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[LIU Liyuan1, 2, LI Changqing1, 2, JI Guanglei3, LU Longfei1, 2, YANG Xiaobin2, 4, CHANG Lirong1, 2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>LIU Liyuan1, 2, LI Changqing1, 2, JI Guanglei3, LU Longfei1, 2, YANG Xiaobin2, 4, CHANG Lirong1, 2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202212015&flag=1]]></guid><cfi:id>43</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Genetic Parameters and Genetic Progress of Growth Traits in a Landrace Pig Population]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202210013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Four genetic parameters including days to 100 kg (DAYS_100), average daily gain (ADG_100), 
loin muscle area (LMA_100) and average back fat thickness (BFT_100) at 100 kg body weight in a Landrace pig population 
were estimated, and the correlations between traits as well as genetic and phenotypic progress of the four traits were analyzed, 
which could provide a basis for the genetic improvement of the target population.【Method】Records of growth traits of 
Landrace pigs were collected in a core breeding pig farm in Guangxi from 2002 to 2020. A fixed effect analysis on the factors 
affecting the growth traits of Landrace pigs was conducted by R software. In addition, the genetic parameters of the four traits were estimated with DMU software and a multi-trait animal model. Furthermore, the genetic correlations and phenotypic 
correlations between these traits, genetic progress and phenotypic progress were evaluated.【Result】The estimated heritability 
for the four growth traits of Landrace pigs, including DAYS_100, ADG_100, LMA_100 and BFT_100 were 0.399, 0.391, 0.433 
and 0.421, respectively, and all of them had medium to high heritability. Both genetic correlation and phenotypic correlation 
between DAYS_100 and ADG_100 were significantly negative, with correlation coefficient -0.997 and -0.992, respectively. In 
general, the phenotypic trend of DAYS_100 was rising while the phenotypic trends of ADG_100, LMA_100 and BFT_100 were 
declining; the genetic trends of ADG_100 and BFT_100 showed an overall upward trend while the trends of DAYS_100 and 
LMA_100 were generally downward.【Conclusion】The four growth traits of Landrace pigs are medium-high heritability traits, 
therefore, their genetic progress can be accelerated through direct selection. There is a strong correlation between DAYS_100 
and ADG_100. The management of phenotypic measurement of pig farms and the selection of target traits for pig population 
breeding have an important impact on the performance of growth traits. In addition, the improvements in farm production 
management and changes in breed structure may influence genetic progress.]]></description>
<pubDate>2022/11/29 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[YAN Qiaochu1
, LIN Qing1
, FENG Xueyan1
, SU Zhanqin1
, WU Xibo2
,
SI Jinglei2
, ZHANG Zhe1
, LI Jiaqi1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>YAN Qiaochu1
, LIN Qing1
, FENG Xueyan1
, SU Zhanqin1
, WU Xibo2
,
SI Jinglei2
, ZHANG Zhe1
, LI Jiaqi1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202210013&flag=1]]></guid><cfi:id>42</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Detection, Isolation and Identification of Streptococcus suis in A Slaughterhouse in Dongguan]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202210014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Streptococcus suis (SS) is an important porcine pathogen and a zoonotic pathogen. Among 
various types, Streptococcus suis type 2 (SS2) has the strongest pathogenicity. It can not only cause acute sepsis, meningitis, 
arthritis and even death in pigs, but also lead to human infection and death. In order to evaluate the transmission risks of SS in slaughterhouses, the sampling, detection, isolation and identification of SS were carried out in a slaughterhouse 
in Dongguan.【Method】The synovial fluid and tonsils of 40 pigs were collected for PCR detection and SS isolation. The 
isolated strains were used for morphology, physiology and biochemistry identification, 16S rDNA PCR amplification, typing 
PCR detection and antibiotic sensitivity test.【Result】The nucleic acids extracted from the synovial fluid and tonsils of 40 
pigs were detected by PCR. In 40 synovial fluids, the positive rate of SS was 22.5%, but no SS2 was detected. In 40 tonsil 
samples, the positive rate of SS was 32.5%, in which the positive rate of SS2 was 7.5%. Three strains of SS were isolated from 
80 tonsil and synovial fluid samples of 40 pigs. The PCR results showed that one of the three strains was SS type 4, and the 
genotype was epf-mrp+gdh+gapdh+fbps-orf2+sly-; while the other two strains were SS type 9, and the genotypes were epfmrp+gdh+gapdh+fbps-orf2+sly-and epf-mrp-gdh+gapdh+fbps-orf2+sly+, respectively. The drug sensitivity test showed that 
the three SS strains were resistant to penicillin G, lincomycin, polymyxin B and sulfaisoxazole, and sensitive to enrofloxacin, 
ciprofloxacin, spectinomycin and amoxicillin.【Conclusion】The results suggest that there are a large number of recessive 
infection of SS in the pigs of the slaughterhouse, and there is a risk of human infection by SS2.]]></description>
<pubDate>2022/11/29 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[Streptococcus suis; epidemiology; antibiotic sensitivity test; slaughterhouse]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>Streptococcus suis; epidemiology; antibiotic sensitivity test; slaughterhouse</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202210014&flag=1]]></guid><cfi:id>41</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effect of Noni on Growth and Immunity Performance of Hainan Black Goatj]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202210015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】In order to improve the growth and immune performance of Hainan black goat, a new 
type of immune synergist was developed with Noni (Morinda citrigolia L.) as the material to promote the healthy 
breeding and sustainable industry development of Hainan black goat.【Method】Fifty-four healthy Hainan black goats 
were selected and randomly divided into 6 groups. The experimental period was 60 d, and the growth and immune 
performance of each group were measured on the 0 d, 30 d and 60 d of the experiment, respectively.【Result】On the 60th 
day of the experiment, the average body weight and daily gain of the high-dose Noni group were significantly different from 
those of the Astragalus polysaccharide and other groups, and increased with the increase of the supplemental dose. In 
addition, there was no significant difference in serum total protein and globulin between Astragalus p. group and Noni group. 
On the 30th day and 60th day of the test, the serum levels of IgG, IL-2 and IFN-γ in the high-dose Noni group were slightly higher than those in the other treatment groups, which were significantly different from those in the control group, and they were 
increased with the increase of the additive dose.【Conclusion】Adding 20 g Noni powder every day and feeding for 60 days 
is the ideal feeding scheme, and the daily gain of Hainan black goat can reach 97.27 (±20.87) g. The levels of IgG, IL-2 and 
IFN-γ are 49.17 (±3.52) g/L, 136.24 (±12.77) ng/L and 112.72 (±10.94) ng/L, respectively. Noni can significantly improve 
the growth and immune performance of Hainan black goat. Therefore, Noni has a broad application prospect in the green and 
healthy breeding and industrial development of Hainan black goat.]]></description>
<pubDate>2022/11/29 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[LIU Xianxi1
, WU Fu 1, JIANG Qicheng2
, LIU Bang 2, CHEN Shuai 2, ZHOU Hailong 2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>LIU Xianxi1
, WU Fu 1, JIANG Qicheng2
, LIU Bang 2, CHEN Shuai 2, ZHOU Hailong 2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202210015&flag=1]]></guid><cfi:id>40</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Growth Characteristics of Different Exopalaemon carinicauda Populations Under Low and High Salinity Conditions]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202210016&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】In order to explore the growth characteristics of Exopalaemon carinicauda under different 
salinity conditions, the growth patterns and relationship between body length and body weight were compared to provide 
references for the healthy breeding of E. carinicauda.【Method】The growth parameters of E. carinicauda under lowsalinity (5.0 ‰ ±1.0 ‰ ) and high-salinity (22.0 ‰ ±1.0 ‰ ) conditions were analyzed, including body length and body weight.【Result】(1) The growth trends of E. carinicauda populations from Fengxian and Ninghai were rather similar, 
which grew faster under low-salinity model. The average daily increments of body length and body weight of Fengxian 
population were 0.0203 cm and 0.0194 g under high-salinity model, while those were 0.0252 cm and 0.0265 g under lowsalinity model; the average daily increments of body weight of Ninghai population under high-salinity model and low-salinity 
model were 0.0183 g and 0.0254 g, which were slower than those of Fengxian population; (2) The relationship between body 
length and body weight of E. carinicauda populations from Fengxian and Ninghai was correlated as the power function, 
and their regression equations were: Fengxian population under high-salinity model: W=0.024L2.731 (R2
=0.971), Fengxian 
population under low-salinity model: W=0.023L2.765 (R2
=0.992), Ninghai population under high-salinity model: W=0.022L2.807
(R2
=0.993), Ninghai population under low-salinity model: W=0.028L2.643 (R2
=0.990). All of their b values were less than 
3, which showed allometric growth patterns; (3) There were linear relationships between body length and breeding time in 
Fengxian and Ninghai populations under two salinity models, while power function relationships between body weight and 
breeding time under low-salinity model, in which the index function of Fengxian population was: Y=0.500e0.014t
 (R2
=0.971), 
and that of Ninghai population was: Y=0.017t
1.0148 (R2
=0.977); (4) The trends of fullness of E. carinicauda populations were 
similar, which were highest at the beginning of the experiment and then gradually decreased to the lowest in the 150 day-age.
【Conclusion】In general, the experimental populations under low-salinity breeding environment grow significantly faster than 
they do under high-salinity breeding environment, and low-salinity model is more conducive to their growth and reproduction.]]></description>
<pubDate>2022/11/29 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[ZHANG Nianguo1
, ZHOU Yuhua1
, YU Fei2
, ZHOU Wenyu1
, HOU Wenjie1
, LIU Benwei1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>ZHANG Nianguo1
, ZHOU Yuhua1
, YU Fei2
, ZHOU Wenyu1
, HOU Wenjie1
, LIU Benwei1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202210016&flag=1]]></guid><cfi:id>39</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Cloning and Expression Characteristics Analysis of the Aquaporin Gene EHP00_492 from Enterocytozoon hepatopenaei]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202308016&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The aquaporin gene EHP00_492 from Enterocytozoon hepatopenaei (EHP) was cloned and its expression characteristics in EHP mature spores was analyzed, which provided a theoretical basis for studying the function of EHP aquaporin. 【Method】The complete EHP00_492 gene was cloned from Ent. hepatopenaei. The sequence characteristics of EHP00_492 was analyzed by bioinformatics methods. Recombinant expression vector pCold-TF-EHP00_492 was constructed and transformed into Escherichia coli to induced fusion protein expression for preparing the rabbit polyclonal antibody of EHP00_492. The expression and subcellular localization characteristics of EHP00_492 in EHP mature spores were analyzed by Western blot and indirect immunofluorescence assay. 【Result】EHP00_492 gene is 729 bp in length, encoded 242 amino acids. EHP00_492 is rich in leucine, with a predicted molecular weight of 25 kD. It has no signal peptide, but involving six transmembrane domains, the MIP conserved domain and two aquaporin conserved NPA/G motif. In addition, EHP00_492 has a high homology with other microsporidian aquaporins, and the phylogenetic tree analysis result shows that EHP00_492 is closely related with EBI_27080 protein from Ent. bieneusi. The three-dimensional structure of EHP00_492 is highly similarity with the identified aquaporin NbAQP from Nosema bombycis and EcAQP from Enc. cuniculi by AlphaFold analysis, which is speculated that EHP00_492 is a potential aquaporin. EHP00_492 is a 21 kD-protein in the mature spores of EHP by Western blot analysis. And the subcellular localization analysis showed that EHP00_492 protein was localized at the spore wall of EHP mature spores. 【Conclusion】In this study, the sequence and structure characteristics of EHP00_492 protein, as well as its phylogenetic relationship with other microsporidian aquaporins, and the expression and subcellular localization characteristics of EHP00_492 protein in EHP mature spores were preliminary clarified, which provided basis for further study on the function of EHP aquaporins.]]></description>
<pubDate>2023/10/20 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[LI Chenxi1, WU Huixin1,2, WU Yujiao1, CHEN Jie1, MENG Xianzhi1, PAN Guoqing1, LONG Mengxian1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>LI Chenxi1, WU Huixin1,2, WU Yujiao1, CHEN Jie1, MENG Xianzhi1, PAN Guoqing1, LONG Mengxian1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202308016&flag=1]]></guid><cfi:id>38</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Research Advances in Purine Phosphoribosyltransferases of Protozoan Parasites]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202308017&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[Parasitic protozoa are single-celled organisms that have adapted to live in cells of humans and animals. The protozoan parasites include Leishmania spp., Trypanosoma spp., Plasmodium spp., Toxoplasma gondii, Cryptosporidium spp., and Eimeria spp., which can cause protozoal diseases that seriously endanger human and animal health and cause huge economic losses in the livestock industry. Parasite development and reproduction after invasion of the host require a large number of purine nucleotides, and the corresponding purine bases are catalyzed by purine phosphoribosyl transferase to generate the corresponding purine nucleotides. Purine phosphoribosyltransferases (PPRT) are important metabolic enzymes involved in the ribophosphorylation of purine bases and are present in many protozoan parasites. Adenine phosphoribosyltransferase (APRT) and hypoxanthine-guanine-xanthine phosphoribosyltransferase (HGXPRT) belong to PPRT. APRT and HGXPRT convert adenine, hypoxanthine, guanine, and xanthine into adenosine-5 ′-monophosphate (AMP), inosine-5 ′-monophosphate (IMP), guanosine-5′-monophosphate (GMP), and xanthosine- 5′-monophosphate (XMP), respectively. Purine nucleotides are 
involved in many functions as components of DNA and RNA, as enzyme cofactors in metabolic pathways, as sources of energy in protozoan parasites. Purine phosphoribosyltransferases are key enzyme in the purine salvage pathway of parasitic protozoa, which is significantly different from the de novo synthesis pathway of the host. In recent years, purine phosphoribosyltransferases have become the interesting research hotspot as an antiparasitic drug candidates target, with major progress in the screening of compounds against protozoan parasites. This review focuses on the basic characteristics, biological functions, inhibitors screening and application of purine phosphoribosyltransferases in parasitic protozoa of Leishmania spp., Trypanosoma spp., Plasmodium spp., and Toxoplasma gondii, so as to provide reference for the research of drug targets and the screening of new inhibitors against parasitic protozoa.]]></description>
<pubDate>2023/10/20 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[YU Zhihui1,2, FANG Siyun3, SUN Mingfei1, QI Nanshan1, LIU Wenjun2, LI Juan1, HU Junjing1, LIAO Shenquan1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>YU Zhihui1,2, FANG Siyun3, SUN Mingfei1, QI Nanshan1, LIU Wenjun2, LI Juan1, HU Junjing1, LIAO Shenquan1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202308017&flag=1]]></guid><cfi:id>37</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Association Between SNP of GH Gene and  Comb Traits of Ningdu Yellow Chicken]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202307014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The Paper aims to explor the relationship between single nucleotide polymorphisms (SNP) of 5'-flanking and Intron 1 in growth hormone (GH) gene and the comb traits of Ningdu yellow chicken, and find molecular markers for the comb traits selection of local chicken breeds. 【Method】The comb trait data at seven different week age of 499 Ningdu yellow chickens were recorded including comb height, comb length, comb thickness, wattle length, wattle thickness and number of comb teeth. These traits were associated with SNPs in the 5'- flanking and Intron 1 regions of GH gene using statistical analysis system (SAS). 【Result】A total of 13 SNPs were significantly associated with comb height trait (P < 0.05), including C1272225T, G1272611A, C1271211T, C1270902T, A1270769G, G1270640C, T1270578C, G1270503C, T1270451C, C1270032T. A1270769G was significantly correlated with the comb height traits at seven different week age. G1272611A was significantly associated with the comb height traits at five different week age. A total of 10 SNPs were significantly associated with comb length trait (P < 0.05), including C1272225T, G1272611A, C1271211T, C1270902T, A1270769G, G1270640C, T1270578C, G1270503C, T1270451C, C1270032T. C1271211T was significantly correlated with the comb length at six different week age. G1272611A was significantly correlated with the comb length at five different week age. The locus G1272611A was significantly associated with both comb length and height trait at five consecutive weeks, which had two genotypes in this population, GG and GA, comb height or length of GG genotype individuals were significantly higher than those of GA genotype. 【Conclusion】G1272611A could be used as a candidate molecular marker for selection of Ningdu yellow chicken comb traits.]]></description>
<pubDate>2023/9/20 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[MA Jing’e, ZENG Chengyao, ZHOU Min, TAN Yuwen, ZHU Xuenong, RAO Yousheng, XU Jiguo]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>MA Jing’e, ZENG Chengyao, ZHOU Min, TAN Yuwen, ZHU Xuenong, RAO Yousheng, XU Jiguo</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202307014&flag=1]]></guid><cfi:id>36</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Isolation and Identification of Fowl Adenovirus Serotype 2 and Establishment of A Disease Model for SPF Chickens]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202307015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】A disease model of fowl adenovirus serotype 2 (FAdV-2) was established to provide a refference for evaluating the immunogenicity and vaccine effectiveness of FAdV-2.【Method】 The samples suspected to be infected with avian adenovirus were isolated and identified. The genetic evolution of the Fiber and Hexon gene sequences of the isolates was analyzed. The nucleotide and amino acid sequences of the isolated strain and other FAdV representative strains were analyzed using DNAStar and MEGA7.0. After determining that the isolated virus was FAdV-2, the pathogenicity of the isolated strain was analyzed in 6-week-old SPF chickens under different infection routes and different infection doses in animal experiment. The best infection model and infection dose were selected to establish the FAdV-2 pathogenesis model. 【Result】One FAdV-2 strain was successfully isolated from the infected chicken tissue samples, named KM strain. Genetic evolution analysis shows that KM strain and GX01 strain belong to the FAdV-2 of avian adenovirus species D of group Ⅰ. The homology analysis shows that the nucleotide similarity of Fiber gene and Hexon gene between KM strain and GX01 strain is 
98.9% and 99.3%, and the amino acid similarity is 98.9% and 98.8%, respectively. The pathogenicity of intravenous injection was higher than that of intramuscular injection, and the chickens showed obvious hepatitis hydropericardium syndrome (HHS). The infection route of the KM strain model was intravenous injection, and the infection dose was 108.0 TCID50. The chickens died after infection, with a mortality rate of 50%. Obvious pathological changes were found in the dead chickens at necropsy, and the chickens were positive for cloaca virus shedding at 1 d post infection. The established KM strain disease model provides a certain reference for vaccine development and vaccine evaluation, which will effectively prevent and reduce the transmission of FAdV-2.【Conclusion】It is verified that FAdV-2 can cause hepatitis hydropericardium syndrome, and a pathogenesis model of FAdV-2 infection in SPF chickens is established for the first time.]]></description>
<pubDate>2023/9/20 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[HU Xinyu1，2, SUN Minhua2, ZENG Qinghang1，2, XIE Zimin2, YUAN Zhaoxia1, LIAO Ming2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>HU Xinyu1，2, SUN Minhua2, ZENG Qinghang1，2, XIE Zimin2, YUAN Zhaoxia1, LIAO Ming2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202307015&flag=1]]></guid><cfi:id>35</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Prokaryotic Expression of Doublesex Gene From Macrobrachium rosenbergii and Protein Purification]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202307016&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Doublesex, a member of the DMRT gene family, plays a key role in controlling sex-specific differentiation. To clone the Open Reading Frame (ORF) of Macrobrachium rosenbergii Doublesex (MrDsx) gene, construct the recombinant plasmid and induce its expression, purify and obtain the recombinant protein MrDsx, will provides basic information for further study of MrDsx function. 【Method】Based on the ORF sequence of MrDsx, the recombinant plasmid pET-32a-MrDsx was obtained by ligating the specific PCR product with the expression vector pET-32a (+). The recombinant plasmid pET-32a-MrDsx was transformed into the competent cells of Escherichia coli BL21 (DE3) to construct the prokaryotic expression cells of MrDsx. The isopropyl-beta-D-thiogalactoside (IPTG) was used to induce the expression of positive transformed cells, and the induced recombinant protein was detected by SDS-PAGE, Western blot and mass spectrometry analysis.【Result】Using the gonad cDNA as template, a single DNA band with the size of about 660 bp was obtained by the specific PCR primers of MrDsx. And the MrDsx ORF sequence was confirmed by sequencing. The recombinant protein MrDsx could be obtained from the transformed competent cells after induction by IPTG at 37℃ for 4 h. When the final concentration of IPTG was 0.1-0.5 mmol/L, the expression level of recombinant protein MrDsx reached its peak. Soluble analysis showed that the recombinant protein MrDsx was mainly expressed in the form of inclusion bodies. After solubilization with 8 mol/L urea, Ni purification, and dialysis renaturation, a single band was detected at the relative molecular weight of 55 kD by SDS-PAGE and Western blot, indicating that the recombinant protein MrDsx could be specifically recognized by His-tag mouse monoclonal antibody. The results of mass spectrometry analysis showed that the fragmented peptide was consistent with the theoretical amino acid sequence, and the matching degree was 100%. 【Conclusion】The recombinant plasmid pET-32a-MrDsx was successfully obtained by prokaryotic expression of MrDsx. High purity active MrDsx protein can be obtained by the dissolution, purification and renaturation. This study provides a preliminary basis for the subsequent study of MrDsx gene function, screening of interacting proteins and elucidation of sex regulation mechanisms in M. rosenbergii.]]></description>
<pubDate>2023/9/20 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[WANG Ruirui1,2, MA Keyi3]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>WANG Ruirui1,2, MA Keyi3</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202307016&flag=1]]></guid><cfi:id>34</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Seasonal Variation Characteristics of the Symbiodiniaceae Community Associated with Acropora pruinosa from Weizhou Island]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202307017&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The global coral reef ecosystem is rapidly degrading due to the combined effects of climate change and human activities, which highlights the need to explore the environmental adaptation mechanisms of corals. The environmental adaptability of coral hosts is closely related to symbiotic Symbiodiniaceae, however, the ecological effects of Symbiodiniaceae driven by seasonal variation still need to be further understood.【Method】An environment-sensitive coral Acropora pruinosa from the Weizhou Island, Beibu Gulf was taken as the research object to carry out fixed-point sampling and environmental data monitoring, and analyze the density, community composition, chlorophyll a concentration and correlation with environmental variables of symbiotic Symbiodiniaceae in four different quarters.【Result】The coral reef area was characterized by high temperature and strong light in summer and low temperature and weak light in winter. The density of Symbiodiniaceae and the concentration of chlorophyll a showed the characteristics of decreasing in summer and increasing in winter. The subclade C1 always occupied the dominant position (79.35%-82.97%), and the relative abundance of background subclades such as C1ca, C1p, C72, and Cspc significantly varied with seasonal variation. There were significant differences in the structure of Symbiodiniaceae communities between summer and winter. The contribution of surface seawater temperature (SST) and photosynthetically active radiation (PAR) to the changes in Symbiodiniaceae community structure was higher than chlorophyll a, Kd490, and particulate organic carbon.【Conclusion】The environmental characteristics of high temperature and strong light in summer and low temperature and weak light in winter on Weizhou Island regulate the seasonal changes of Symbiodiniaceae density and chlorophyll a concentration by affecting the photosynthesis of Symbiodiniaceae. Moreover, the physiological characteristics of the C1 subclade ensure that A. pruinosa can adapt to the environment of Weizhou Island. In addition, the SST and PAR affect the abundance changes of the background Symbiodiniaceae, leading to significant differences in community structure.The correlation analysis showed that the SST and PAR were the main factors driving the changes of density, stable symbiosis (subclade C1), and community structure ofSymbiodiniaceae. This study took A. pruinosa as an example to analyze the micro-ecological characteristics of coral-associated Symbiodiniaceae adapting to seasonal variation, providing a new understanding about the adaptation of environment-sensitive corals to environmental changes.]]></description>
<pubDate>2023/9/20 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[XU Yongqian1, CHEN Biao1,2,3, QIN Liangyun1, CHEN Jinni1, NIU Tianyi1, LIANG Jiayuan1,2,3]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>XU Yongqian1, CHEN Biao1,2,3, QIN Liangyun1, CHEN Jinni1, NIU Tianyi1, LIANG Jiayuan1,2,3</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202307017&flag=1]]></guid><cfi:id>33</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effect of Compound Plant Essential Oil on Performance and Intestinal Microflora of Yellow-Feathered Broilers]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202306014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The trial explores the effect of compound plant essential oil from the nutmeg and basill on the 
performance and intestinal microflora of yellow-feathered broilers.【Method】3 942 1-day-old Jiangcun yellow broilers were 
divided into three treatments groups (low-dose compound essential oil group, high-dose compound essential oil group and blank 
control group). The experiment lasted for 60 days. At the age of 60 days, all broilers were weighed, and the number of survival 
chickens, the number of dead and unqualified chickens, feed consumption of each replicates were recorded, and the percentage 
of survival chickens, weight gain and feed-gain ratio were calculated. At the same time, 5 chickens were randomly selected 
from each group and killed with neck cut. The contents of jejunum and cecum were respectively extracted, and bacterial nucleic 
acid was extracted, the 16S rDNA V3-V4 variable region was high-throughput sequencing to analyze the intestinal microflora 
of jejunum and cecum of broilers.【Result】The feed-gain ratio of the high dose group and the low dose group was 2.40, 
which was significantly lower than that of the blank control group (2.71, P<0.01), and the feed conversion rate was increased by 
11.4%. The results of jejunum microflora analysis showed that Firmicutes were the dominant bacteria at phylum level followed 
by Actinobacteriota, and Lactobacillus was the dominant bacteria at genus level. The relative abundance of Verrucomicrobiota 
in jejunum in high-dose and low-dose groups was significantly lower than that in blank control group (P<0.05). The relative 
abundance of Aeriscardovia, Bifidobacterium and Weissella in jejunum of compound essential oil low-dose group was 
significantly higher than that of blank control group (P<0.05). The results of cecal microflora analysis showed that Firmicutes 
and Bacteroidetes were dominant bacteria at phylum level. The relative abundance of Clostridia-vadinBB60-group and Alistipes
in high-dose and low-dose compound essential oil groups was significantly lower than that in blank control group (P<0.05), 
and that of Clostridia-UCG-014 in high-dose compound essential oil group was significantly lower than that in blank control 
group (P<0.05). The relative abundance of Bacteroides in high dose compound essential oil group was significantly higher than 
that in blank control group and low dose compound essential oil group (P<0.05), and the relative abundance of Ruminococcustorques-group in low dose compound essential oil group was significantly higher than that in blank control group (P<0.05).
【Conclusion】Feeding nutmeg and basil compound essential oil can promote the growth of beneficial bacteria in jejunum and 
cecum of yellow-feathered broilers, inhibit the proliferation of harmful bacteria, maintain the balance of bacteria, and promote 
intestinal health, and obviously improve the performance of yellow-feathered broilers.]]></description>
<pubDate>2023/8/17 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[HE Jianan 1,2, HE Shunian 3,4, KANG Huahua 2
, FU Dejia 1,2, FAN Zhihong 3,4, 
ZENG Xianjun 3,4, CHEN Jie 3,4, PENG Xinyu 2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>HE Jianan 1,2, HE Shunian 3,4, KANG Huahua 2
, FU Dejia 1,2, FAN Zhihong 3,4, 
ZENG Xianjun 3,4, CHEN Jie 3,4, PENG Xinyu 2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202306014&flag=1]]></guid><cfi:id>32</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Exploration of GES-induced masculinization ofGambusia affi nis Based on Single Cell Level]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202306015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】To investigate the molecular mechanism of sex reversal in female Western mosquitofish 
(Gambusia affinis) under the effect of gestodene (GES) by studying the development of gonadal cell differentiation and 
gene expression changes involved in the process of sex reversal in female mosquitofish at the single-cell transcriptome 
level.【Method】Sexually mature female mosquitofish were exposed to 500 ng/L GES after culture and domestication for 
2 months , and samples were taken at the 4th and 8th week to observe morphological changes, and single-cell suspensions 
were made from the gonads of normal female controls, male controls, and samples exposed for 4 and 8 weeks, respectively, for single-cell transcriptome sequencing and analysis.【Result】The morphology of GES-treated female mosquitofish showed 
gonopodium, and single-cell transcriptome sequencing revealed completely different cellular composition of gonads between 
male and female mosquitofish. The gonads of male mosquitofish consist mainly of fibroblasts, male germ cells, epithelial cells 
and primordial germ cells, whereas the gonads of female mosquitofish consist mainly of endothelial cells, fibroblasts, oocytes 
and primordial germ cells. After 4 and 8 weeks of exposure, a small amount of male germ cells appeared in the gonads of 
female mosquitofish, and the number of primordial germ cells increased significantly. Further analysis revealed that several 
crucial genes related to the sex-differentiation were differentially expressed in primordial germ cells. Cell trajectory analysis 
showed that some primordial germ cells showed characteristics of differentiation towards males, in which 2 477 genes showed 
different expression patterns in two different different directions of differentiation. The results of KEGG pathway enrichment 
analysis revealed that signaling pathways, such as Toll-like receptor, TGF-beta, Notch, etc., were involved in the process of 
reversal of primordial germ cells towards maleness. Glycine, serine, threonine, cysteine, methionine metabolism, amino sugar 
and nucleotide sugar metabolism and other pathways were also related to this process. 【Conclusion】After exposure to GES 
solution, female mosquitofish showed morphologically masculinized anal fins, and some primordial germ cells in the gonads were 
transformed to male germ cells. Differential expression of amh, fabp3, dmrt1, wt1a, wt1b, cyp19a1a and fgf16, which are key 
genes for sex determination, was observed in the primordial germ cells. Toll-like receptor, TGF-beta, Notch, and some amino 
acid metabolism signaling pathways were involved in the process of sex reversal.]]></description>
<pubDate>2023/8/17 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[FENG Tingyin, DENG Xikai, XING Ke]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>FENG Tingyin, DENG Xikai, XING Ke</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202306015&flag=1]]></guid><cfi:id>31</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Study on the Growth Performance of the Hybrid Progeny Black Soldier Fly Derived from Wuhan Strain×Bioforte Strain]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202306016&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】In order to study whether hybrids of the black soldier fly (Hermetia illucens L.) have growth 
advantages, the hybrid progeny of Wuhan strain and Bioforte strain and the difference of growth performance between Wuhan 
strain and Bioforte strain were studied. 【Method】Experimental strains F and F´ were obtained by cross and transverse cross between Wuhan strain and Bioforte strain, respectively. The growth performance , eclosion time and oviposition time of F 
and F´ were measured and compared with those of Wuhan strain and Bioforte strain. 【Result】The eclosion time of F strain 
and F´ strain was 30.3 d and 35.3 d, and the oviposition time was 36.6 d and 40.3 d, respectively. The eclosion and oviposition 
time of F strain were 5 d shorter than that of Wuhan strain, 9 d shorter than that of Bioforte strain, and the oviposition time of 
F´ strain was 1.4 d shorter than that of Wuhan strain. The eclosion time was 4 d shorter than that of Bioforte strain, and the 
oviposition time was 5.4 d shorter than that of Bioforte strain. In terms of feed utilization, the apparent digestibility of F strain, 
F´ strain, Wuhan strain and Bioforte strain were 59.53%, 59.25%, 58.05% and 58.10%, ingested feed conversion efficiency 
were 4.11%, 4.58%, 3.41% and 3.74%, and the feed-weight ratio were 8.58, 7.78, 10.5 and 9.05, respectively. Compared 
with Wuhan strain and Bioforte strain, the apparent digestibility of F strain was significantly higher, and the eclosion time and 
oviposition time were significantly lower. The digested feed conversion efficiency, apparent digestibility and ingested feed 
conversion efficiency of F´ strain were significantly higher than those of Wuhan strain and Bioforte strain, while the feed-weight 
ratio and protein content were significantly lower than those of Wuhan strain and Bioforte strain. There were no significant 
differences in the number of egg laid, quality of egg mass, or hatching rate among F strain, F´ strain and the Wuhan strain and 
Bioforte strain.【Conclusion】F strain and F´ strain were superior to Wuhan strain and Bioforte strain in feed utilization, but 
the protein content was slightly lower than Wuhan strain and Bioforte strain; The number of egg laid, egg mass and hatching rate 
of F strain, F´ strain were well maintained at the level of Wuhan strain and Bioforte strain; F strain had an advantage in growth 
cycle, and its eclosion time and oviposition time were shorter than Wuhan strain and Bioforte strain.]]></description>
<pubDate>2023/8/17 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[QIN Chengjie1
, HU Bin2,3, YE Canquan1
, ZHU Jianfeng2
, LI Xueling1
, TIAN Ling4
, HUANG Zhijun4
, HU Wenfeng2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>QIN Chengjie1
, HU Bin2,3, YE Canquan1
, ZHU Jianfeng2
, LI Xueling1
, TIAN Ling4
, HUANG Zhijun4
, HU Wenfeng2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202306016&flag=1]]></guid><cfi:id>30</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Biological Characteristic of HA and NA Genes of H9N2 Avian Influenza Virus in Guangdong Province]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202305012&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objection】This study aimed to monitor the genetic variation and molecular evolutionary trends of H9N2 avian influenza virus (AIV) in Guangdong, and provide reference data for the evolutionary analysis and prevention and control of H9N2 AIV in China. 【Method】The HA and NA genes of three H9N2 AIV strains isolated from Guangdong in 2022 were amplified, cloned, and sequenced. The DNAStar and PhyloSuite software were used for sequence assembly and alignment. The MEGA, Megalign, NetNGlyc 1.0 Server, and other software were used for genetic evolution, percent identity, receptor binding, protein activity, drug resistance, and glycosylation site analysis of their nucleotide and amino acid sequences. 【Result】The phylogenetic tree showed that the HA gene of the three isolates belonged to the clade h9.4.2.5 and the NA gene belonged to the clade 1. However, the nucleotide homology with the early strain was only 81.6%~91.7% and 88.2%~91.2%, formed new clade named clade h9.4.2.5c and clade 1.2, respectively. The nucleotide and amino acid sequence analysis revealed that the HA cleavage site was PSRSSR ↓ GLF. The receptor binding site underwent I155T, H183N, A190T/V, T212I, Q226L, and Q227M mutations. The glycosylation site underwent 218N non-glycosylation and acquired new glycosylation site at 313N. The NA stalk showed nine nucleotides deletion at positions 187-195 (ACAGAGATA), leading to the loss of three amino acids at positions 63-65 (TEI). The absorbtion site of NA on red blood cells showed K/E/S368N and D369N mutations. No related mutations to neuraminidase activity and drug resistance were found at the E119, D151, R152, R224, E276, R292, and R371. 【Conclusion】The HA and NA genes of the three H9N2 AIV isolated from Guangdong in 2022 had evolved into a new subgroup. Some mutaitons suggest that prevalent H9N2 in Guangdong may enhanc adaptability of mammals and its antigenicity has changed. Moreover, it has not yet acquired resistance to durgs oseltamivir and zanamivir.]]></description>
<pubDate>2023/7/5 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[ZENG Qinghang1,2, LIU Yang2, SUN Minhua2, HU Xinyu2, XIE Zimin2, ZHANG Minxia2, YUAN Zhaoxia1, LIAO Ming2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>ZENG Qinghang1,2, LIU Yang2, SUN Minhua2, HU Xinyu2, XIE Zimin2, ZHANG Minxia2, YUAN Zhaoxia1, LIAO Ming2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202305012&flag=1]]></guid><cfi:id>29</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Analysis of Virulence and Drug Resistance of Glaesserella parasuis Serotype 2]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202305013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Glaesserella parasuis (GPS) is the pathogen that causes Glässer’s disease in pigs. It is one of the most serious bacterial pathogens that harm pig industry. There are many serotypes of this bacterium, and the crossprotection between different serotypes is weak. Serotype 2 is a moderately virulent strain, and the proportion of clinical isolates has been increasing, resulting in increasingly serious harm in recent years. GPS serotype 2 was isolated from nursery pigs in a large-scale pig farm in Henan Province and a series of tests were conducted, and provided reference for scientific prevention and control of the disease. 【Method】In this study, lung, trachea, blood and other samples of GPS clinically suspected cases pigs were collected. Bacterial isolation and purification, Gram staining microscopy, routine PCR, guinea pig pathogenicity test, AGAR diffusion drug sensitivity test and other methods were used. Morphological observation, identification, serotyping, virulence gene detection, drug-resistant gene detection, pathogenicity and drug resistance of suspected bacteria were studied. 【Result】A strain of GPS was isolated from lung samples and identified as serotype 2. The strain carried virulence genes of vta1, vta2, vta3, wza, ompP2, nanH, cdtA, cdtB, cdtC and espP2, and showed strong virulence to guinea pigs. The strain carried drug-resistant genes aadA1, strA, strB, aphA1, tet(B), sul2, and had strong resistance to penicillin G, kanamycin, amicacin, streptomycin, tetracycline, oxytetracycin, and cotrimoxazole. It had good sensitivity to ceftiofurme, ceftazidime, 
amoxicillin, ampicillin, levfloxacin, ofloxacin, ennofloxacin and ciprofloxacin, and had significant clinical effect. 【Conclusion】The isolates of GPS serotype 2 have strong virulence, can pass through the blood-brain barrier, and have obvious multi-drug resistance. The results of this study provide reference for the epidemiological investigation of GPS serotype 2, pathogenic mechanism, and the formulation of clinical prevention and control measures.]]></description>
<pubDate>2023/7/5 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[WANG Zhifang, XU Yindi, ZHU Wenhao, JIAO Wenqiang, ZHANG Qingxian, LI Haili, XU Feng, WANG Keling]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>WANG Zhifang, XU Yindi, ZHU Wenhao, JIAO Wenqiang, ZHANG Qingxian, LI Haili, XU Feng, WANG Keling</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202305013&flag=1]]></guid><cfi:id>28</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effects of Acute Freshwater Stress on Tissue Structure of Gill, Heart, Spleen and Liver of Juvenile Eleutheronema tetradactylum]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202305014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】To investigate the effects of acute freshwater stress on the tissue structure of gill, heart, spleen and liver of juvenile Eleutheronema tetradactylum, in order to provide reference for exploring low salinity culture and breeding of improved varieties.【Method】Juvenile E. tetradactylum, temporarily reared at salinity 9, were placed directly into an aquarium with a salinity of 0. Live samples were taken at 2, 6, 12, 24, 48 and 72 h. Histological methods were used to study the gill, heart, spleen and liver structures of juvenile E. tetradactylum under different durations of acute fresh-water stress. 【Result】In juvenile E. tetradactylum, the length of the gill lamellae tended to increase gradually, the width of the gill lamellae decreased and then increased, the spacing of the gill lamellae increased and then decreased, and the number of mitochondria-rich cells continued to increase when exposed to acute freshwater stress. The size of the myocardial transverse muscle, the size of the cardiomyocyte gap and the size of the interstitial blood vessels all showed a trend of increasing and then decreasing. The peak of myocardial transverse muscle size was at 12 h of stress, and the peak of myocardial interstitial and interstitial blood vessel size was at 24 h of stress. The white marrow area and marginal zone area gradually increased from 0 to 24 h of stress, and the colour of melanin macrophages also gradually deepened, reaching a peak at 24 h of stress and then gradually decreasing. 72 h later, the red pulp, marginal zone, white pulp and melano-macrophages were roughly restored to the level of the control group. In the control group, the hepatocytes were large, polyhedral, with rounded nuclei and centrally located, and some hepatocytes had 2-3 nuclei; the hepatic plate was clearly defined; the hepatic blood sinusoids were normally distributed among the hepatocytes. During 24 h of stress, the hepatic plate structure was gradually blurred and the gap between the hepatic sinusoids was gradually widened, reaching a peak at 24 h of stress; during 24-72 h of stress, the hepatic plate structure gradually regained clarity, the gap between the hepatic blood sinusoids was gradually reduced and some nuclei lost their intrinsic morphology.【Conclusion】 Under freshwater stress, the tissues (gills, heart, spleen) of juvenile E. tetradactylum exhibit stress responses within 24 h, and adapt to a low salt environment after 24 h. However, some liver cells are still difficult to recover from acute freshwater stress. This indicates that juvenile E. tetradactylum have some ability to adapt to low salt stress.]]></description>
<pubDate>2023/7/5 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[XU Shuyu1,2, OU Youjun1,3, WEN Jiufu1, LIN Xin1,4, LI Jia’er1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>XU Shuyu1,2, OU Youjun1,3, WEN Jiufu1, LIN Xin1,4, LI Jia’er1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202305014&flag=1]]></guid><cfi:id>27</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Study on Genetic Diversity of Qilin Chicken Based on Complete Mitochondrial DNA D-loop Sequences]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202304012&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was carried out to evaluate genetic diversity level of Qilin chicken inferred from complete mitochondrial DNA (mtDNA) D-loop sequences in order to clarify the bread formation of Qilin chicken.【Method】Three populations (yellow, white and black-feathered Qilin chickens) and eight indigenous chicken breeds were used for direct sequencing with PCR products of complete mtDNA D-loop region, and these sequences were then used to calculate the genetic variation and neutral test, to construct median networks of haplotypes and to investigate the breeding history of Qilin chickens.【Result】The length of mtDNA D-loop complete sequence ranged from 1 231 to 1 232 bp. The content of C+G (39.8%) was lower than that of T+A (60.2%). The total nucleotide diversity of Qilin chickens was 0.00630±0.00054, which was significantly higher than that of each of the eight indigenous chicken breeds. The haptype diversity and nucleotide diversity in yellow, white and black-feathered Qilin chickens were 0.777±0.076, 0.816±0.060, 0.710±0.057 and 0.00699±0.00115, 0.00662±0.00090, 0.00546±0.00062, respectively. The level of genetic diversity was positively correlated with population size. The K2P genetic distance and net genetic distance between Qilin chickens and eight indigenous chicken breeds ranged from 0.008 to 0.009 and 0.003 to 0.005, respectively, which were greater than those between the eight indigenous chicken breeds. A total of 45 mutation sites were detected in 90 samples of Qilin chickens. Seventeen haplotypes were defined in Qilin chickens, with eight exclusive haplotypes and haplotype diversity of 0.773±0.039. The numbers of haptypes of yellow, white and black-feathered Qilin chickens were 12, 7 and 5, respectively. Nineteen haplotypes were defined in eight adjacent indigenous chicken breeds, and no haplotype was shared with Qilin chickens. The number of haptypes of Hetian chicken (8) was greater than that of other chickens, and Ningdu yellow chicken only had three haptypes.The neutral test indicated that all chickens used in this study did not show obvious population expansion at the breed level, with the exception of black-feathered Qilin chickens. The haplotypes of Qilin chickens were distributed in clades A, B, C and E, and the dominated haplogroups were B (30.0%) and E (64.4%). The haplotypes of the eight indigenous chicken breeds were mainly distributed in clades A and B.【Conclusion】Unique haptypes indicated that Qilin chickens had an independent breed formation history, and the relatively 
higher level of genetic diversity of this breed will facilitate its protection and utilization.]]></description>
<pubDate>2023/6/8 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[LI Weina1,2,3, GUO Meihui1, WEN Jintian1, WENG Zhuoxian1,2,3,CHEN Jiebo3, DU Bingwang1,2,3, HUANG Xunhe1,2,3]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>LI Weina1,2,3, GUO Meihui1, WEN Jintian1, WENG Zhuoxian1,2,3,CHEN Jiebo3, DU Bingwang1,2,3, HUANG Xunhe1,2,3</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202304012&flag=1]]></guid><cfi:id>26</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Investigation of Rhabdovirus Infection in Hybrid Snakehead (Channa argus ♂ × Channa maculata ♀ ) in the Pearl River Delta]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202304013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Hybrid snakehead is an important special aquatic fish in China. In order to find out the infection status of hybrid snakehead rhabdovirus (HSHRV), the rhabdovirus disease of hybrid snakehead cultured in the Pearl River Delta was investigated from March 2021 to February 2022.【Method】The parents, fry and water samples of hybrid snakehead were randomly sampled in breeding stage, and these were randomly sampled at fixed time in culture stage and sampled when the outbreak of the virus. A total of 333 samples were collected during the whole monitoring period. Mixed samples of mung bean-sized liver, spleen and kidney were stored in liquid nitrogen for RNA extraction. And reversetranscribed cDNA was used as template to detect rhabdovirus by real-time fluorescence quantitative PCR (qPCR).【Result】The main clinical symptoms of rhabdovirus-infected fish were that the fish swam irregularly and spin. Although there was no obvious symptom on the body surface, the liver, spleen, kidney, intestine and swim bladder became red or swollen. The qPCR results revealed that the average positive rate of HSHRV was 13.21%, which was mainly detected from April to November. Among them, the positive rate of samples detected in August was as high as 23.91%. The average positive rate of HSHRV in the culture stage (17.01%) was significantly higher than that in the breeding stage (3.26%). In terms of fish body size, 32.20% of the fry were most susceptible to HSHRV infection within 10 cm, mainly detected from March to September, while 9.52% of the fish of more than 20 cm were least susceptible to HSHRV infection.【Conclusion】The positive rates of HSHRV in hybrid snakehead in breeding stage and cultivating stage as well as in different size are determined, which provide references for further understanding the epidemic regularity of hybrid snakehead rhabdovirus disease, with a view to providing data support for the prevention and control of hybrid snakehead rhabdovirus disease.]]></description>
<pubDate>2023/6/8 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[WEN Caiyi1, JIANG Biao1, SUN Yanxin1, HUANG Weimin1, MA Zhuang1, LIANG Zhiyuan2, LIU Chun1, HU Xiong2, SU Youlu1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>WEN Caiyi1, JIANG Biao1, SUN Yanxin1, HUANG Weimin1, MA Zhuang1, LIANG Zhiyuan2, LIU Chun1, HU Xiong2, SU Youlu1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202304013&flag=1]]></guid><cfi:id>25</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Study on Breeding of Babylonia areolata Applying Artificial Compound Feed with Sthenoteuthis oualaniensis as Protein Source]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202304014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Sthenoteuthis oualaniensis is a mollusk with abundant resources and high development potential in the South China Sea. The study was carried out to investigate the feasibility of S. oualaniensis used as an alternative protein source of artificial compound feed for Babylonia areolata.【Method】Seven artificial compound feeds for B. areolata with different mass fractions (33.1%, 43.1%, 53.1%, 63.1%, 73.1%, 83.1%, and 100%) of S. oualaniensis were designed and fed to B. areolata larvae with initial body weight of 0.37 (± 0.03) g. The control group was fed with fish, and the 
experiment lasted for 90 days. The daily average weight gain and growth rate, feed coefficient, survival rate and yield per unit area were used as indexes to evaluate the breeding effect.【Result】In the treatment with 63.1% of S. oualaniensis, the daily gain of B. areolata was 0.034 g and growth rate was 9.31%, which were significantly higher than those of the control (0.029g and 7.96%); the feed coefficient was 1.33, which was significantly lower than that of the control (2.27); the yield per unit area of 3 168.6 g/m2 was higher than that of 2 856.8 g/m2 in control group, but both there were no significant differences between these two treatments. The daily gain and growth rate of B. areolata in the other groups were lower or significantly lower than that of the control. The survival rate of B. areolata in the groups with the addition of S. oualaniensis were lower than that in the control, and there were no significant differences between the treatment of adding 63.1% or more of S. oualaniensis and the control.【Conclusion】The use of the artificial compound feed for B. areolata does not require removal of residual bait or residues, which can reduce the workload of breeding and reduce the dependence on trash fish and protect offshore fishery resources. In terms of daily average weight gain, daily growth rate and feed coefficient, it is feasible to add S. oualaniensis to artificial compound feed of B. areolata, and the reference value of mass fraction is 63.1%. The application of S. oualaniensis as the main protein source can become an important direction for the development of B. areolata feed in the future.]]></description>
<pubDate>2023/6/8 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[ZHAO Wang1,2, CHEN Xu1,2, CHEN Mingqiang1,2, HUANG Xingmei2,DENG Zhenghua1,2, WEN Weigeng1,2, WANG Jiangyong3]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>ZHAO Wang1,2, CHEN Xu1,2, CHEN Mingqiang1,2, HUANG Xingmei2,DENG Zhenghua1,2, WEN Weigeng1,2, WANG Jiangyong3</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202304014&flag=1]]></guid><cfi:id>24</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Mining Candidate Genes for Litter Size Traits in Large White - Landrace Crossbred Sows Based on Whole Genome Resequencing]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202303014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The litter size is an important economic index for breeding sows. The candidate genes related to the regulation of litter size traits in sows were explored, and the genetic mechanism of commercial pig fertility represented by binary hybrid sows was investigated, in order to lay a foundation for further application of molecular breeding. 【Method】 Comparative analysis was conducted on two groups of extremely high and extremely low yielding Large White - Landrace sows by using whole genome resequencing, and selection elimination analysis was used to obtain Fst and θπ signal intersection as a high selection region for gene annotation and candidate gene screening.【Result】A total of 8 040 367 SNPs were found in two groups of samples, 50.6% of which were located in the intergenic region, 45.2% in the intron region, and only 1.0%, 1.1%, and 0.6% in the exon region, non-coding region 3' end, and 5' end, respectively. Among the SNP located in the exon region of the genome, 28 879 were non-synonymous variants, accounting for 35.0%. Extracting SNP loci located in the exon region, intron region, and non-coding regions at the 3' and 5' ends for gene annotation, a total of 1 136 differentially expressed genes were screened between the two groups of samples. KEGG and GO enrichment analysis on differential genes were conducted, and a total of 10 candidate genes that may affect reproductive performance and litter size traits were screened out, including EPHA4, SMAD7, GLI2, LRP1B, WT1, BAX, BCAT2, IL1B and FAF1. The gene functions mainly involved embryonic development, cell apoptosis, and etc【Conclusion】The Large White - Landrace binary sows have experienced significant artificial selection on the litter size trait, and the candidate genes affecting the litter size trait of binary sows have been preliminarily excavated. These genes play a role in the physiological activities related to pig reproductive performance, and mainly in the period of apoptosis of sow egg cells and the beginning of pregnancy to particpate in regulation.]]></description>
<pubDate>2023/5/5 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[QU Zixiao, YANG Shuaipeng, ZHU Xiangxing, TANG Dongsheng]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>QU Zixiao, YANG Shuaipeng, ZHU Xiangxing, TANG Dongsheng</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202303014&flag=1]]></guid><cfi:id>23</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Study on Annual Growth Characteristics of Pond-culture Bahaba taipingensis Juvenile]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202303015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was conducted to explore the annual growth characteristics of pond-cultured rich the basic data of culture biology so as to provide references for the artificial pond culture and breeding of Bahaba taipingensis, and further protect and use the germplasm resources of B. taipingensis.【Method】Taking the B. taipingensis juveniles artificially cultured for less than one year as research materials, the growth parameters such as body length and body mass of pond-cultured B. taipingensis juveniles were measured regularly within one year, and the annual growth characteristics of B. taipingensis juveniles were analyzed.【Result】The initial average body length and average body mass of juveniles of B. taipingensis were increased from 4.56 (±0.54) cm to 30.22 (±4.32) cm and 10.63 (±0.91) g to 1 085.73 (±328.55) g after 365 days of growth. The body length was increased by 562.16%, with an average daily growth of 0.07 cm. The specific growth rate of body length ranged from 0.08%/d to1.08%/d, and the variation coefficient of body length ranged from 7.83% to 14.31%. The body mass was increased by 10 117.06 %, with an average daily growth of 2.95g. The specific growth rate of body mass ranged from 0.68%/d to 2.77%/d and the variation coefficient of body mass ranged from 8.53% to 33.37%. The specific growth rate and variation coefficient of body mass were greater than those of body length, and the annual change of fatness ranged from 2.21% to 11.18%. The body length of B. taipingensis juvenile was shown to be correlated with body 
mass in a power function: m=0.3783L2.2363, (R2=0.9705, b= 2.2363 ＜ 3), with a negative allometric growth stage. There was linear relationship between the total length (LT) and body length (L): LT =1.1918L+0.2809 (R2=0.9986). There was quadratic function between the total length (LT) and age (t): LT=-0.0002t2+0.159t+4.6652 (R2=0.9791), quadratic function between body length (L) and age (t): L=-0.0002t2+0.1294t+3.7988 (R2=0.9865), and quadratic function between body mass (m) and age (t): m=0.0076t2+0.0618t+28.915 (R2=0.9956).【Conclusion】The B. taipingensis juvenile under artificial pond-cultureconditions grow well within one year, being at negative allometric growth stage.]]></description>
<pubDate>2023/5/5 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[MO Jiehua1, LIU Wenyu2, LU Binqian3, LU Weihua1, LU Changsheng3, LI Chunzhi1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>MO Jiehua1, LIU Wenyu2, LU Binqian3, LU Weihua1, LU Changsheng3, LI Chunzhi1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202303015&flag=1]]></guid><cfi:id>22</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Prokaryotic Expression and Purification of OmpA of Klebsiella pneumoniae Isolated from Mauremys mutica]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202303016&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was conducted to clone the outer membrane protein A (KpOmpA) gene of Klebsiella pneumoniae isolated from Mauremys mutica, construct the recombinant plasmid and induce its expression, purify and obtain the recombinant protein KpOmpA, which would lay a foundation for preparing the vaccine to prevent K. pneumoniae infection. 【Method】Using the DNA as the template, KpOmpA gene was cloned and connected with pET-32a expression vector, and the constructed recombinant plasmid pET-32a-KpOmpA was transformed into the competent cells of E. coli BL21 (DE3). After induction by IPTG, the expression of recombinant protein KpOmpA was detected by using SDS-PAGE and the dissolubility was analyzed. Then the KpOmpA was purified by Ni-IDA agarose resin column. In addition, purified recombinant protein was identified by Western blot.【Result】The CDS sequence of KpOmpA was 1 071 bp in length with presumptive encoding 356 amino acids. The OmpA was highly conserved which shared more than 99% sequence homology with those from other K. pneumoniae strains. The pET-32a-KpOmpA was identified and confirmed by double enzyme digestion and sequencing. The sequencing result confirmed that there was no code shift or mutation, indicating that the recombinant plasmid was successfully constructed. The transformed competent cells of E.coli BL21 were induced by IPTG with the final concentration of 1 mmol/L at 37 ℃ for 4 h, SDS-PAGE assay showed that KpOmpA was expressed abundantly in the form of inclusion bodies, with a molecular weight of about 57 kD. The recombinant protein was purified and obtained by Ni-IDA agarose resin column. Western blot results show that there was a 57 kD band, indicating that recombinant protein KpOmpA could be recognized by His-tag mouse monoclonal antibody.【Conclusion】The recombinant plasmid pET-32a-KpOmpA was successfully constructed and expressed in E. coli expression system. The recombinant protein KpOmpA with high purity was obtained, laying a foundation for preparing the vaccine to prevent K. pneumoniae infection.]]></description>
<pubDate>2023/5/5 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[MO Chaodi, QIN Chenghuan, DAI Longxi, HAN Pengfu, JI Yuexin, WEI Youchuan]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>MO Chaodi, QIN Chenghuan, DAI Longxi, HAN Pengfu, JI Yuexin, WEI Youchuan</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202303016&flag=1]]></guid><cfi:id>21</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Research Progress in the Application of Arginine in Broiler Production]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202303017&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[Arginine is a functional essential amino acid in broilers with a variety of physiological functions. The research progress of arginine in growth, carcass quality, meat quality, intestinal health, immunity and antioxidation in broilers was reviewed, and the appropriate requirement of arginine and its interaction with lysine were analyzed. At the same time, the application of arginine substitutes guanidinoacetic acid and N-carbamoylglutamic acid in broiler production were summarized. Appropriate level of arginine in diet is beneficial to improve growth performance, carcass quality and meat quality, repair intestinal injury, maintain normal intestinal barrier function, regulate intestinal microflora, regulate the expression of intestinal digestive enzymes and amino acid transporters, and improve immunity and antioxidation capacity. Insufficient or excessive arginine in the diet is not conducive to the normal growth and development of broilers. The arginine requirement of broilers is affected by breed type, sex, feeding stage, ratio of arginine to lysine and so on. At present, most of the studies mainly focus on fast-growth white-feathered broilers, but there are few reports on the arginine requirement of yellow-feathered broilers in China, and the research results are quite different. As substitutes for arginine, guanidinoacetic acid and N-carbamoylglutamic acid have different effects on broilers. Guanidinoacetic acid does not completely replace arginine, but dietary supplementation with guanidinoacetic acid can reduce the use of arginine in energy metabolism and make it more useful for muscle growth and other physiological functions. N-carbamoylglutamic acid can increase the synthesis of endogenous arginine, increase the content of arginine in vivo and promote the growth of broilers. With the deepening of the studies on the effect and mechanism of arginine and its substitutes on broilers, the requirements of arginine will be more accurate, and be widely used in broiler production. It will help to further improve the production performance of broilers and improve feed utilization efficiency.]]></description>
<pubDate>2023/5/5 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[TAN Shujun1,2, JIANG Shouqun2, HUANG Yunmao1, JIANG Zongyong2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>TAN Shujun1,2, JIANG Shouqun2, HUANG Yunmao1, JIANG Zongyong2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202303017&flag=1]]></guid><cfi:id>20</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Epidemiological Investigation, Isolation and Identification of Canine Distemper Virus in Parts of Henan and Hebei Provinces from 2019 to 2020]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202302011&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was carried out to investigate the prevalence and genetic diversity of canine distemper virus (CDV) in central regions of China in recent years.【Method】A total of 459 samples of dogs, minks, raccoon dogs and foxes were collected randomly from parts of Henan and Hebei provinces from 2019 to 2020. Then, RTPCR detection, isolation and identification of strains, sequencing of hemagglutinin (H) gene and phylogenetic analysis were performed.【Result】87 CDV positive samples are detected, with a positive rate of 18.96%. The positive rate of CDV in dogs is highest (37.77%) in autumn and highest in minks, raccoon dogs and foxes in July. CDV occurs in animals of different ages, and young animals are more susceptible. A wild strain is isolated and identified from the diseased samples and named HB19-1. Phylogenetic analysis of H gene sequence indicates that HB19-1 belongs to the Asia-1 genotype, and it has the closest nucleotide homology with KJ994343 from GenBank. And its nucleotide and amino acid homologous rates with the vaccine strain Onderstepoort (GeneBank Accession No.: AF378705) are 91.3% and 90.2%, respectively.【Conclusion】A CDV strain HB19-1 is isolated and obtained during the epidemiological investigation of CDV in parts of Henan and Hebei provinces from 2019 to 2020. The phylogenetic analysis indicates that HB19-1 belongs to the Asia-1 genotype and has low homology with the vaccine strain. The research results can provide a basis for the prevention and control of CDV and vaccine selection and application in parts of Henan and Hebei involved in the epidemiological investigation.]]></description>
<pubDate>2023/4/21 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[ZHANG Le，YU Xiaohang，WANG Yihe ，YANG Jiamei ，ZHAO Lili，DUAN Ming]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>ZHANG Le，YU Xiaohang，WANG Yihe ，YANG Jiamei ，ZHAO Lili，DUAN Ming</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202302011&flag=1]]></guid><cfi:id>19</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Progress in Structure and Function of GP5 Protein of Porcine Reproductive and Respiratory Syndrome Virus]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202302012&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[Porcine reproductive and respiratory syndrome (PRRS) is a highly contagious disease caused by porcine reproductive and respiratory syndrome virus (PRRSV). As an RNA virus，there are extensive variations in the genome of PRRSV，of which NSP2 and ORF5 genes are predominant. As the strains of different lineages have characteristic nucleotide sequences in ORF5，ORF5 can be used for the typing of PRRSV. ORF5 encodes the GP5 protein. GP5 protein contains multiple epitopes，which can interact with cellular receptors thus affect viral infection and replication. In recent years，molecular epidemiological investigations on PRRSV tend to reveal amino acid mutations unique to different strains. These mutations usually occur in the signal peptide coding region，non-neutralizing epitope，neutralizing epitope and transmembrane region of GP5 protein. Therefore，some studies have correlated these mutation data with the virulence and neutralization capacity of the virus. Some researchers tried to speculate about the conformational or functional changes in GP5 protein by mutation and explain this link from the structural basis of protein interaction. The tertiary structure of GP5 cannot be observed by traditional high-resolution cryo-EM and X-ray crystallography method，therefore，bioinformatics methods become the only option for related researches. In addition，GP5 can be used for the development of genetic engineering vaccines. This article reviews bioinformatics studies on GP5 protein in recent years，and summarizes the effects of GP5 protein on virus infection，replication，virulence and neutralization as well as the performance evaluation of GP5-based genetic engineering vaccines.]]></description>
<pubDate>2023/4/21 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[KANG Pu1,2，ZHAO Mengpo2，HUANG Yuhao3，LYU Zongji1，WANG Xiaohu2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>KANG Pu1,2，ZHAO Mengpo2，HUANG Yuhao3，LYU Zongji1，WANG Xiaohu2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202302012&flag=1]]></guid><cfi:id>18</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Progress of Molecular Epidemiology of Newcastle Disease in China]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202302013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[Newcastle disease (ND) is one of the diseases that seriously damage the health of poultry and is widely prevalent worldwide. It is characterized by high fever, respiratory distress, dysentery, neurological disorders, and mucosal hemorrhage, seriously threatening the growth and reproduction of poultry and production of poultry meat. Newcastle disease is caused by Newcastle disease virus (NDV), which has been prevalent for nearly 100 years and can infect more than 200 species of poultry, including chickens, ducks, geese and pigeons. It has many genotypes, and the variable genotypes bring great difficulties to the prevention and control of ND. The migration of birds plays an important role in the cross-border spread of the virus, resulting in Newcastle disease outbreaks in various regions of China, bringing huge economic losses to the poultry industry and seriously restricting the healthy development of the poultry breeding industry. In view of the wide distribution of NDV and the strong host mobility, it makes an important epidemic disease threatening the poultry breeding industry. Therefore, to understand the prevalence of NDV in China, the study analyzes its molecular epidemiology and the spatial and temporal distribution of each genotype, and it is found that ND in China is mainly distributed in southern China, such as Guangxi and Guangdong, eastern China, such as Jiangsu and Anhui, and northwestern China, such as Shaanxi and Gansu. The highly pathogenic Class Ⅱ strains isolated from these regions include multiple genotypes, with genotype Ⅱ and 
genotype Ⅶ strains being the main prevalent strains. The collation and analysis of NDV prevalence data provide reference data for epidemic prevention control, and vaccine development.]]></description>
<pubDate>2023/4/21 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[LIAN Cong, WEN Xiaohui, LYU Dianhong, GAO Xiaopeng, JIA Chunling, ZHOU Xiurong, CHANG Qi, LUO Shengjun]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>LIAN Cong, WEN Xiaohui, LYU Dianhong, GAO Xiaopeng, JIA Chunling, ZHOU Xiurong, CHANG Qi, LUO Shengjun</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202302013&flag=1]]></guid><cfi:id>17</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Construction of DF-1 Cell Line of Stably Expressing Cas9 Protein Mediated by Lentivirus and Verification of Its Activity]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202302014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The chicken fibroblast cell line (DF-1) of stably expressing Cas9 protein was screened, and combined with the reporter vector system based on single strand annealing (SSA) repair mechanism, the Cas9 nuclease activity in the established DF-1 cell line was detected.【Method】The lentiviral vector plasmid carrying Cas9 protein was transfected into 293T cells packaging lentivirus together with the helper plasmid. DF-1 cells were infected with lentivirus Cas9 supernatant, and DF-1 cells with stable Cas9 protein expression were obtained through antibiotic screening. The expression of Cas9 protein in positive screened DF-1 cells was verified by polymerase chain reaction (PCR) and Western blot. The sgRNA sequence of chicken ovalbumin (OVA) gene was selected and and the annealing products were cloned into pYP152 to construct sgRNA expression vector. Primers were designed at both ends of the sgRNA target site to amplify the target fragment of OVA gene. The target fragment was cloned into mCherry-SSA reporter vector pCMV-SSA-mCherry-Hind Ⅲ to destroy the expression of mCherry protein. Then the sgRNA expression vector and mCherry-SSA reporter vector were co-transfected into DF-1 cells of stably expressing Cas9 protein. Finally, the repair of mCherry expression by different stable transformants was analyzed by fluorescence microscope.【Result】27 DF-1 cell lines of stably expressing Cas9 protein were obtained after antibiotic screening, cell genome was stably transfected by PCR amplification, and the results showed that these cells contained Cas9 protein sequences. The results of Western blot assay showed that all the stable transfection cell lines expressed Cas9 protein; after co-transformation of sgRNA expression vector and mCherry-SSA reporter vector, fluorescence microscope observation showed that all the selected stable transfection cell lines could restore the expression of mCherry protein in the report vector.【Conclusion】The DF-1 cell line of stably expressing Cas9 protein with cleavage activity was successfully established, which could provide basic materials for the subsequent research on chicken functional genes on the DF-1 cell line of stably expressing Cas9 protein.]]></description>
<pubDate>2023/4/21 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[LI Xiaojiao1，ZHU Xinyu1，ZOU Xian2，YAN Xia2，HE Yanhua2，LUO Chenglong2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>LI Xiaojiao1，ZHU Xinyu1，ZOU Xian2，YAN Xia2，HE Yanhua2，LUO Chenglong2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202302014&flag=1]]></guid><cfi:id>16</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Preparation Method of Chitosan Oligosaccharide and Chitin Oligosaccharide and Their  Application in Aquaculture]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202302015&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[People expect pollution-free and residue-free green aquatic products earnestly. However, in recent years, with the rapid expansion of aquaculture farming density and scale, aquatic animal diseases are frequent, and problems such as strain resistance caused by the abuse of antibiotics have gradually emerged, which bring challenges to the sustainable and green development of aquaculture. Chitosan oligosaccharides and chitin oligosaccharides due to non-toxic, nonharmful, and residue-free characteristics, with a variety of bioactive functions and a wide range of sources, is gradually valued and studied by many scholars. Chitosan oligosaccharides are oligosaccharides obtained from chitin or chitosan by enzymatic, chemical or physical methods. Chitin oligosaccharides are degradation products of chitin or acetylation products of oligochitosan. At present, experimental studies on chitosan oligosaccharides and chitin oligosaccharides as green feed additives, vaccine adjuvants and aquatic products water retention and freshness have been carried out, and they have broad development space and application prospects in aquaculture. Based on this, this review summarizes the preparation methods of chitosan oligosaccharides and chitin oligosaccharides, and discusses the application of Chitosan oligosaccharides and chitin oligosaccharides in aquatic animal feed additives, vaccine adjuvants and aquatic products water preservation and freshness, highlighting their biological activities in regulating aquatic animal immunity, promoting growth, anti-oxidation and affecting fish body composition, with a view to providing references for further research and industrial application of Chitosan oligosaccharides and chitin oligosaccharides in aquaculture.]]></description>
<pubDate>2023/4/21 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[LU Chunlan, WANG Bei]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>LU Chunlan, WANG Bei</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202302015&flag=1]]></guid><cfi:id>15</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Application Effect Analysis of Single-step Genomic Selection in Multi-population of Yorkshire Pig]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202311011&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Exploring the difference in prediction accuracy between genome selection (GS) and traditional BLUP methods in the presence of a large number of non-genotype reference populations. Assessing the application effect of GBLUP and ssGBLUP in the joint evaluation to provide recommendations for joint evaluation.【Method】Two straits of days to reach 100 kg (DAYS_100) and the average backfat thickness at 100 kg (BFT_100) were analyzed in six Yorkshire populations, and the heritability and genetic correlation of the two traits were estimated. Exploring the prediction accuracy of BLUP, GBLUP and ssGBLUP models in different populations and combined populations.【Result】(1) In F population, the heritability of BFT_100 was only 0.071, while in other populations it was between 0.205 and 0.383. The heritability of DAYS_100 in six populations ranged from 0.258 to 0.598. (2) The genetic correlation between the two traits in D population was 0.211, however, in other populations, the genetic correlations were negative, ranging from -0.462 to -0.200. (3) For DAYS_100 trait, the GBLUP model showed the best prediction accuracy in B, C, E, and F populations. For BFT_100 trait, the ssGBLUP model had the best prediction accuracy in populations A, B, and C, while the GBLUP model performed better in populations D and E. (4) The connectedness rating (CR) between F and A population was 3.096 %. In F population, single-step genomic selection using combined reference populations can improve the prediction accuracy of BFT_100 trait. 【Conclusion】When the number of genotyped individuals in the population exceeds 500 and the proportion is above 7%, the prediction accuracy of GBLUP or ssGBLUP model will be higher than that of BLUP model. ssGBLUP model can be used to improve the prediction 
accuracy of low heritability traits in joint population that the CR reaches 3% between population.]]></description>
<pubDate>2024/1/9 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[LU Yujin1 2, HUANG Zhen3, XU Huazhao1, SHI Shaolei2, ZHOU Jie3, ZHANG Zhe2, XIE Shuihua1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>LU Yujin1 2, HUANG Zhen3, XU Huazhao1, SHI Shaolei2, ZHOU Jie3, ZHANG Zhe2, XIE Shuihua1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202311011&flag=1]]></guid><cfi:id>14</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Identification of VRTN Gene Mutation in Liang Guang Small Spotted Pigs and Analysis of Its Effects on Carcass and Meat Quality Traits]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202311012&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The mutations in the VRTN gene g.20311_20312ins291 and g.19034A>C have been found to influence the number of thoracic vertebrae in lean meat pigs. These mutations can increase the number of thoracic vertebrae. This study aims to investigate the frequency of VRTN gene mutations in the Liang Guang Small Spotted pig populations and their impact on carcass and meat quality traits, providing reference for molecular breeding of Liang Guang Small Spotted pigs.【Method】Genomic DNA of 69 Liang Guang Small Spotted pigs was extracted and subjected to PCR amplification.Gel electrophoresis and TA cloning were used to detect the mutations at the g.20311_20312ins291 and g.19034A>C loci of the VRTN gene. Carcass and meat quality traits of the population were recorded, and the animals were grouped based on their mutation status. Statistical analysis was performed using t-tests.【Result】Among the population, 5 individuals were identified as carrying heterozygous mutations at both loci, indicating that the two mutations may be closely linked, with a frequency of 3.62% for both mutations. Statistical analysis indicated that the VRTN linked mutation allele did not significantly affect the number of ribs in Liang Guang Small Spotted pigs. However, they did affect carcass and meat quality traits. In terms of carcass traits, the VRTN gene heterozygous mutations significantly increased body length by 4.6% (P < 0.05) and body weight by 9.6% (P < 0.01), increased body slant length by 3.5%, but the difference was not significant. The backfat thickness and skin thickness were increased by 6.5% and decreased by 3.7%, respectively, but without significant differences. In terms of meat quality traits, the brightness L, chromaticity index a and b values of the heterozygous mutants increased by 0.01%, 14.7%, and 8.4%, respectively. The water loss rate significantly increased by 51.2% (P < 0.05). The difference in changes of shear force and pH value are not significant.【Conclusion】The VRTN gene mutations g.20311_20312ins291 and g.19034A > C are detected in the Liang Guang Small Spotted pig populations, but their frequencies are relatively low. These two mutations may be tightly linked. The VRTN gene heterozygous mutations will not increase the number of thoracic vertebrae in Liang Guang Small Spotted pigs, but they improve carcass traits to some extent while also have adverse effects on meat quality traits. Future breeding of Liang Guang Small Spotted pigs needs to balance the impact of VRTN gene mutations on carcass traits and meat quality traits.]]></description>
<pubDate>2024/1/9 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[JIANG Weilun1, ZENG Jianhua2, SONG Deqing2, LIU Xiaohong1, CHEN Yaosheng1, HE Zuyong1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>JIANG Weilun1, ZENG Jianhua2, SONG Deqing2, LIU Xiaohong1, CHEN Yaosheng1, HE Zuyong1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202311012&flag=1]]></guid><cfi:id>13</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Molecular Marker-Assisted Selection of Green Shank and White Feather Traits in Chickens]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202311013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The shank and feather color, as the characteristic traits of breeds, which remains an important selective trait in the breeding process of new varieties. This study attempts to analyze the formation mechanism of green shank and white feather through molecular biology techniques and establish a molecular marker-assisted selection scheme for the above traits. 【Method】The BCO2 gene was selected as the candidate gene for the formation of green and yellow shank, and the target site on the BCO2 was typed by sequencing to analyze the relationship between the locus and different traits. The MC1R, PMEL17 and TYR encoding extended locus e, dominant white I and recessive white C were used as candidate genes related to white feather, and the recessive white Plymouth Rock breeds, fast-and-big-type white-feather broiler, synthetic recessive white line and hybrid offspring white feather individuals were used as experimental materials. The relevant candidate mutations were genotyped by Sanger sequencing, and the relationship between the polymorphism of related genes and traits was analyzed. 【Result】(1) The green and yellow shank trait is the result of the interaction of melanin in the dermis and the yellow scale in the shank. Mutations in the BCO2 gene are key to the formation of trait of green and yellow shank. The mutations site in 342 bp can be used for molecular marker-assisted selection of correlated traits. Purification can be achieved by eliminating CT genotype individuals. (2) The recessive white gene locus of synthetic recessive white line is cc, which is caused by a nonrecessive white mutation in the offspring white feathers. (3) The hybrids white feather offspring all carry the dominant white allele I encoded by the PMEL17 gene, all of which are heterozygous genotype Ii. (4) The hybrid white feather offspring all carry the E and ER alleles encoded by the MC1R gene. (5) The dominant white mutant alleles I, E and ER alleles are all derived from fast-and-big-type white-feather broiler. 【Conclusion】The green shank trait is caused by a mutation in the BCO2 gene that controls the skin color. The SNP located at 342 bp of the amplified fragment can be used for molecular marker-assisted selection for this trait. The formation of white feather has no relation with recessive white mutation, which is mainly due to 
PMEL17 mutations encoding dominant white allele I.]]></description>
<pubDate>2024/1/9 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[CHAI Xuewen1,2, GONG Jishang1,2, CUI Fangfang1, KANG Zhaofeng3, WU Yanping3, ZHOU Min1,2, SUN Xuemei4, XU Jiguo1,2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>CHAI Xuewen1,2, GONG Jishang1,2, CUI Fangfang1, KANG Zhaofeng3, WU Yanping3, ZHOU Min1,2, SUN Xuemei4, XU Jiguo1,2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202311013&flag=1]]></guid><cfi:id>12</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Genetic Diversity Analysis of Four Cultured Xenocypris davidi Populations Based on Mitochondrial D-loop Sequences]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202311014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Xenocypris davidi is a freshwater economic fish, which feeds on humus, organic debris, plankton, and benthic animals, is one of the main fish for stock enhancement in Zhejiang Province. The research is aims to understand the impact of artificial breeding on the genetic diversity of X. davidi, and to provide basic data for the design and implementation of stock enhancement strategies for X. davidi in natural waters.【Method】D-loop region of mitochondrial DNA (mtDNA) of four cultured X. davidi populations from Changxing, Balidian, Shuangpu in Zhejiang Provice and Liling in Hunan Province 
were amplified and sequenced. The genetic diversity of the four populations was studied by sequence analysis.【Result】The mitochondrial D-loop sequence length of the X. davidi was 1 038-1 093 bp, and the base A+T content (65.3%) was significantly higher than the C+G content (34.7%), the average transitions and transversions ratio (TS/TV) was 4.6. A total of 101 mutation sites including 97 reduced information sites were detected in 128 D-loop sequences of X. davidi, and defining 19 haplotypes. Among them, the haplotype numbers of Changxing, Shuangpu, Balidian, and Liling populations were 5, 12, 4, and 2, respectively. The haplotype diversity (h) ranged from 0.226 to 0.915, and the nucleotide diversity (π) ranged from 0.00640 to 0.01433. The genetic distance between different breeding populations was 0.03782-0.88756, the genetic differentiation coefficient among populations was 0.78903 (P<0.01), among which the degree of genetic difference between Changxing and Balidian populations was the lowest, and that of Shuangpu and Liling populations was the highest, and the genetic variation mainly occurred among populations.【Conclusion】There are certain differences in genetic diversity among the four cultured X. davidi populations. The results of the genetic variation and population structure of X. davidi will provide reference data for the comparative study on the genetic diversity of the X. davidi, and contribute to the resource protection.]]></description>
<pubDate>2024/1/9 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[LIU Shili1, CHEN Dawei1,2, ZHENG Jianbo1, CHENG Shun1, JIANG Wenping1, CHI Meili1, XIA Fengbo3, LI Fei1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>LIU Shili1, CHEN Dawei1,2, ZHENG Jianbo1, CHENG Shun1, JIANG Wenping1, CHI Meili1, XIA Fengbo3, LI Fei1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202311014&flag=1]]></guid><cfi:id>11</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Differential Analysis of Gene Expression of Toll-like Receptors and Antimicrobial Peptides in Immune Organs and Tissues of Tibetan and Duroc-Landrace-Yorkshire pigs]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202403011&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】In mammals, Toll-like receptors and antimicrobial peptide genes are important components of the innate immune system, which play a crucial role in fighting against pathogen attacks. The study was conducted to explore the expression differences of Toll-like receptor and antimicrobial peptide genes between different immune organs or tissues in Tibetan pigs and Duroc-Landrace-Yorkshire pigs, with an aim to reveal the potential contribution of these genes to disease resistance and immune response and provide theoretical support for the screening of molecular markers for disease resistance.【Method】The mRNA abundance of Toll-like receptors genes (TLR1-TLR9) and two types of antimicrobial peptide genes (PBD-1 and PR-39) in lungs, mesenteric lymph nodes, inguinal lymph nodes, submandibular lymph nodes and spleens of 6-month-old Tibetan and Duroc-Landrace-Yorkshire pigs were detected by qPCR.【Result】The mRNA expression of Toll-like receptors and antimicrobial peptide genes in most of the immune organs or tissues of Tibetan pigs was significantly higher than that of Duroc-Landrace-Yorkshire pigs. Among them, the mRNA expression of TLR1 and TLR2 in lungs was increased by about 50%, and PR-39 was increased by 2.6 times; the expression of TLR4 in mesenteric lymph nodes was increased by 40%, and the expression of TLR1 and PR-39 was increased by 88% and 3 times, respectively. In the inguinal lymph nodes, the expression of TLR1 and TLR2 was increased by about 2 times, and the expression of TLR9 and PR-39 was increased by 70%, especially, the expression of PR-39 increasing by 7 times; The expression of TLR1, TLR2, TLR4 and TLR7 in submandibular lymph nodes was increased by more than 2 times, and the expression of PR-39 was increased by nearly 7 times, which was similar to that in inguinal lymph nodes; the expression of TLR1 in the spleen rose by 3.5 times, which was similar to that in submandibular lymph nodes. The expression of TLR4 and TLR9 increased by about 50%, and the expression of PR-39 increased by 2.5 times.【Conclusion】Tibetan pigs show higher expression levels of Toll-like receptors and antimicrobial peptide genes in multiple immune organs or tissues compared to Duroc-Landrace-Yorkshire pigs. It is implied that Tibetan pigs may possess stronger innate immunity and be able to generate more effective local or systemic immune responses against pathogenic microbial infections. The results of this study provide important theoretical support for the identification of disease resistance molecular markers and are expected to provide a scientific basis for further improvement of disease resistance in Tibetan pigs and other pig breeds.]]></description>
<pubDate>2024/4/30 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[LI Xinming1,2,3, HONG Chun1,2,3, HUANG Qiuyan2, CHENG Leiyan2, MENG Fanming2, ZHU Xiangxing1,3, TANG Dongsheng1,3, WANG Sutian2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>LI Xinming1,2,3, HONG Chun1,2,3, HUANG Qiuyan2, CHENG Leiyan2, MENG Fanming2, ZHU Xiangxing1,3, TANG Dongsheng1,3, WANG Sutian2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202403011&flag=1]]></guid><cfi:id>10</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Genome Characterization and Sequence Analysis of Porcine Circovirus Type 2 Isolated with High Viral Load]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202403012&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was carried out to understand the genetic evolution of Porcine Circovirus Type 2 (PCV2) strains on a swine farm in Guangdong Province, and enrich molecular epidemiological data of PCV2, in order to provide reference for the selection and development of local PCV2 vaccine candidate strains.【Method】Samples suspected of PCV2 infection were detected by using qPCR methods. A PCV2-positive isolate with a high viral load was found, named GD222858. Genome-wide molecular cloning and genetic evolution analysis were performed with PCR methods. MegAlign software was used to compare the amino acid sequences encoded by the ORF1 and ORF2 genes of the strain with the PCV2 isotype reference strain, and the similarity of the amino acid sequences was analyzed. The DNAStar was used to predicte the Cap protein secondary structure and B-cell epitope of this strain and compare it with the Cap protein antigen index of four vaccine strains DBN-SX07-2 (HM641752), LG (HM038034), SH (HM038027) and ZJ (AY686764). 【Result】Sequencing results showed that the genome length of GD222858 strain was 1 767 bp. The genetic evolution analysis indicated that the strain belonged to the PCV2d subtype. The nucleotide similarity with 82 reference strains at home and abroad ranged from 91.4% to 99.6%. It was closest to the Vietnamese strain Han8 (GenBank accession No.: JQ181600). Multiple specific mutation sites F70Y, F77L, W202R and N256S were found at the Rep protein encoded by ORF1. The Cap protein encoded by ORF2 was relatively conserved. Protean predicted that the potential B cell epitopes presented at amino acid positions 5-18, 24-25, 39-41, 48-49, 57-65, 99, 101, 112-114, 139-140, 145-150, 162-165, 175-181, 188-189, 205-211 and 227-232 of Cap proteins. The Cap protein antigen index of the GD222858 strain was different from that of the four vaccine strains, and the antigen index at the amino acids 45-57, 124-132 and 223-233 was significantly higher than that of the other four vaccine strains, and the difference with the LG vaccine strain (HM038034) was greatest.【Conclusion】The reason for the infection of GD222858 strain in pig herds may be due to specific mutations in multiple sites of the Rep protein and improper selection of vaccine strains.]]></description>
<pubDate>2024/4/30 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[CHANG Xin1,2, JIANG Zhiyong2, BIAN Zhibiao2, XU Minsheng2, YANG Dongxia2, YANG Aobing3, ZHAI Shaolun2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>CHANG Xin1,2, JIANG Zhiyong2, BIAN Zhibiao2, XU Minsheng2, YANG Dongxia2, YANG Aobing3, ZHAI Shaolun2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202403012&flag=1]]></guid><cfi:id>9</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Research Progress on Curcumin Metabolism and Regulation on Reproductive Performance in Poultry]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202403013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[Curcumin is a polyphenolic compound extracted from the rhizomes of ginger plant turmeric and Araceae plant, which is mainly absorbed and metabolized in the intestine, and has the effects of anti-inflammation, antioxidation, anti-apoptosis, anti-aging, neuroprotection, immune regulation and metabolic regulation. Curcumin has poor bioavailability in the body. In order to improve the utilization, it is made into a variety of preparations and matching types , including membrane preparations, fiber preparations, dairy preparations, hydrogel preparations, piperin, semiterpenoids, cyclodextrin, fenugreek dietary fiber, lecithin, and etc. Different preparations and matching types will increase the stability of curcumin products, which can ensure the effective utilization of curcumin. Reproductive regulation in poultry mainly relys on the negative feedback regulation mechanism of the hypothalamic-gonadal axis (HPG axis) to maintain germ cell development. In addition, reproductive performance is susceptible to external conditions, with light having the greatest influence. Melatonin produced by light stimulation has strong antioxidant properties, which can inhibit apoptosis and aging by alleviating mitochondrial dysfunction of ovarian and testicular tissues, and ensure the stability of negative feedback regulatory mechanism of HPG and follicle development under oxidative stress. Serotonin in gut microbiome is a precursor to melatonin, and it can affect the production of melatonin; furthermore, intestinal microbes stimulate afferent nerves in intestinal epithelial cells to directly affect the hypothalamus secretion of gonadotropin-releasing hormone, and finally, estradiol (E2) produced in the intestine can enter the ovarian tissue through the blood and promote ovarian development. Studies have found that curcumin can play a functional role in vivo through short-chain fatty acids produced by intestinal microorganisms, however, whether curcumin affects the reproductive performance of poultry through short-chain fatty acids produced by intestinal microorganisms needs to be explored urgently. In the study, the biological activity, metabolism mode, product development and utilization, and mechanism of action of curcumin reproduction regulation in poultry and related research progress are reviewed, aiming to provide a theoretical reference for the rational application of curcumin as a feed additive in poultry production.]]></description>
<pubDate>2024/4/30 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[WANG Junyan1,2, RUAN Dong2, JIANG Shouqun2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>WANG Junyan1,2, RUAN Dong2, JIANG Shouqun2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202403013&flag=1]]></guid><cfi:id>8</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Establishment of a Cross Priming Amplification Detection Method of Lateolabrax maculatus Iridovirus]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202403014&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Lateolabrax maculatus iridovirus (LMIV) is a serious threat to the safety of Lateolabrax maculatus aquaculture industry, and there are no specific prevention and control drugs. Early diagnosis plays an important role in the prevention and control of LMIV. The study intends to establish a simple, fast and accurate on-site rapid diagnosis method to provide technical support for the primary layer diagnosis of LMIV. 【Method】Cross priming amplification (CPA) was used to design CPA primers for the highly conserved region of ATPase gene of LMIV. The constructed ATPase recombinant plasmid was used as a positive template to optimize the primer concentration ratio, Bst DNA polymerase, Betaine, MgSO4, dNTPs, concentration reaction temperature and time in the reaction system. Combined with the disposable nucleic acid test strip technology, the visual detection of LMIV was established by LMIV-CPA. 【Result】The results showed that the optimal primer concentration ratio was 1.0 μmol/L for the cross-primer CPF, 0.4 μmol/L for the stripping primers F3 and B3, and 0.8 μmol/L for the probe primers B1 (FAM) and B2 (Biotin); Concentrations of MgSO4, Betaine, dNTPs and Bst DNA polymerase were 6 mmol/L, 0.4 mol/L, 0.6 mmol/L and 0.256 U/μL; The reaction temperature was 62 ℃ and the optimum reaction time was 45 min. The amplified product of this experiment was trapezoidal band by gel electrophoresis, the reaction product with probe was detected by a disposable nucleic acid test strip detection device, and the reaction result could be visualized by the presence or absence of a characteristic band within 3 to 5 minutes. LMIV could be detected specifically by this method without cross-reaction with other aquatic viruses and pathogenic bacteria. A total of 156 clinical samples were detected by the LMIV-CPA method and the conventional PCR method. The positive detection rate of LMIV-CPA was 93.30%, and that of the conventional PCR method was 85.83%. The detection limit of LMIV-CPA was 102 copies/µL and the sensitivity was 10 times that of conventional PCR, revealing that LMIV-CPA was better than PCR.【Conclusion】LMIV-CPA detection method does not rely on expensive instruments and professional technicians. It can be applied to the on-site rapid detection of LMIV, which provides technical support for accurate and rapid diagnosis as well as effective prevention and control of LMIV.]]></description>
<pubDate>2024/4/30 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[MA Yanping1, QIN Baotian1, LIANG Xi2, WANG Gang1, HAO Le1, ZHOU Donglai3, LIU Zhenxing1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>MA Yanping1, QIN Baotian1, LIANG Xi2, WANG Gang1, HAO Le1, ZHOU Donglai3, LIU Zhenxing1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202403014&flag=1]]></guid><cfi:id>7</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Construction of Co-expressed Gene Network Associated with Late Pregnancy, Lactation Period and Fecundity Traits in Holstein Cattle Muscle Tissue]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202402010&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The key target genes related to late pregnancy, lactation and fecundity in muscle tissue of Holstein cattle were screened, with a view to providing a theoretical basis for further study of gene function.【Method】The dataset GSE62159 from the GEO database on gene expression and fecundity in the muscle of Holstein cattles during late pregnancy, early lactation, and mid lactation were selected. The weighted gene co-expression network analysis (WGCNA) method was used for gene co- expression analysis, and the obtained modules were associated with late pregnancy, mid lactation and fecundity traits. The genes with connectivity in the top 30 of each module were selected as the Hub genes. The module was analyzed by protein-protein interaction (PPI) network with String website. The gene with the top 30 node connection degree value in PPI network was selected as the core gene, and intersected with Hub gene to obtain the key target genes related to different physiological periods and fecundity in the muscle.【Result】A total of 13 modules were obtained, of which the Green and Blue modules were related to late pregnancy and mid-lactation periods respectively, and the Magenta module was related to fecundity. The functional enrichment analysis of genes in each target module showed that the main functions of genes related to the late pregnancy were substance metabolism, energy metabolism, protein synthesis and secretion, body response to oxidative stress and neurodegeneration; the functions of genes related to mid-lactation included maintenance of stem cell population, protein synthesis and secretion, antigen processing and presentation and occurrence of many diseases; while those related to fecundity included angiogenesis, intrauterine embryonic development and actin filament binding, etc. Three key genes related to late pregnancy, one key gene related to mid-lactation and eight key genes related to fecundity were screened in the muscle of Holstein cattle.【Conclusion】The genes related to and late pregnancy in the muscle of Holstein cattle were identified as EIF5A, ACO2 and EEF1G, and the gene related to mid-lactation as EIF4A2, and the genes related to fecundity as ITGB1, MYH9, TLN1, CAV1, COL4A1, COL4A2, FLNA and HSPG2.]]></description>
<pubDate>2024/3/21 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[TANG Xueying1,2, ZHANG Zhifei2,3, TONG Xiong2,3, CHEN Weidong2,3, MIN Li2, JU Xianghong1, LI Dagang2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>TANG Xueying1,2, ZHANG Zhifei2,3, TONG Xiong2,3, CHEN Weidong2,3, MIN Li2, JU Xianghong1, LI Dagang2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202402010&flag=1]]></guid><cfi:id>6</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effects of High-fat Diet on Alternative Splicing of RNA in Abdominal Fat and Liver Tissues of Broilers]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202402011&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Excessive deposition of abdominal fat reduces disease resistance and slaughter rate of broilers. RNA alternative splicing is an important regulatory modality that affects gene function. Systematic analysis of RNA alternative splicing events during broiler abdominal fat deposition contributes to further understanding of the molecular regulation mechanism of broiler abdominal fat deposition.【Method】The transcriptome sequencing results of liver and abdominal fat tissues in high-fat-fed and normal-fed broilers were used to identify the differentially expressed and alternative spliced genes in abdominal fat and liver tissues during high-fat feeding. Significantly differentially spliced genes were analyzed by GO functional annotation, KEGG pathway enrichment analysis and Metascape enrichment analysis, and visualization processing was conducted for the significantly differential alternative splicing events, then splicing factors regulating these differential splicing 
events were analyzed.【Result】Transcriptome sequencing was performed on the abdominal fat and liver tissues of high-fat-fed broilers and normal-fed broilers. 233 significantly differentially expressed genes and 349 significantly differentially alternative spliced genes were found in the abdominal fat tissues. It was found that the significantly differential spliced genes were mainly enriched in cell proliferation, such as cell division and cell growth, as well as in pathways related to adipogenesis, such as glycerophospholipid metabolism and insulin signaling pathway. In liver tissues, 276 significantly differentially expressed genes and 224 significantly differential spliced genes were found, and the enrichment analysis of the significantly differential spliced genes revealed that these genes were mainly enriched in biological processes related to cellular metabolism such as protein acetylation, intra-protein amino acid acetylation and mRNA processing and also enriched in adipogenesis-related pathways such as insulin signaling pathway, glucagon signaling pathway and AMPK signaling pathway. Among the significantly differential spliced genes in abdominal fat and liver, a total of four candidate genes related to influencing abdominal fat deposition traits in broiler were screened, including GPAT4, RELCH, CYP39A1, and PPARA.【Conclusion】The results preliminarily revealed the patterns of RNA alternative splicing regulation in abdominal fat and liver tissues of broilers under high-fat feeding. It was inferred that high-fat feeding may lead to abdominal fat deposition in broilers by affecting the alternative splicing process of genes, which may activate the proliferation of preadipocytes and the metabolism of adipogenesis-related cells. Regulation of target genes by splicing factors was significantly involved in influencing the process of abdominal fat deposition in broilers.]]></description>
<pubDate>2024/3/21 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[AO Guangyu, CHEN Genghua, XIA Yilin, LUO Wen]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>AO Guangyu, CHEN Genghua, XIA Yilin, LUO Wen</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202402011&flag=1]]></guid><cfi:id>5</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Isolation, Identification and Drug Resistance Research of Klebsiella pneumoniae in Drinking Water from a Chicken Farm]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202402012&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】It aims to investigate resistance phenotypes and resistance genes of Klebsiella pneumoniae in drinking water of chicken farm, and analyze the correlation between them, to provide references for effective prevention of the disease.【Method】Strains of K. pneumoniae were isolated and identified from the drinking water system of a chicken farm. The resistances of the isolated strains to 25 antibiotics were detected by the drug-sensitive disk diffusion method. The presence of common drug-resistance genes in all these strains was detected by PCR. The whole genomes of four strains of K. pneumoniae were sequenced to analyze and predict the carrying of drug resistance genes by the Comprehensive Antibiotic Research Database (CARD).【Result】Nine strains of K. pneumoniae were isolated from the drinking water system of the chicken farm. The results of drug sensitivity testing revealed that these strains were multiple drug resistant, and only susceptive to colistin sulfate. Thirteen common drug resistance genes carried by all nine strains were confirmed by PCR, and consistency between drug resistance phenotypes and genes was observed in 12 resistant genes including β-lactamase resistance genes CMY-1, BlaSHV and BlaCTX-M; carbapenem resistance gene KPC; quinolone resistance genes QnrB, QnrS and QnrA; aminoglycoside resistance gene Aph(3')-Ia; sulfa resistance gene Sul2 and etc; while inconsistency between the presence of Mcr-1 and its corresponding phenotype was observed. A total of 59 resistance genes, involving 31 classes of antibiotics, were carried by four strains of K. pneumoniae through whole genome scanning analysis. Among these, the number of genes resistant to tetracycline antibiotics was maximum, followed by genes resistant to macrolide and fluoroquinolone antibiotics. Additionally, a total of 23 drug-resistant genes were predicted when the coverage and homology were both set at a rate of higher than 98%, including FosA6, CRP, KpnF, KpnFG, KpnE, OqxA, OqsB, SHV-11, Tet(D), CTX-M-27, QnrB65, DHA-1, Aph(4')-Ia, MsrE, QnrB2, Aph(3')-Ia, Sul1, CmlA6, FloR, AadA12, Ant(3')-IIa, ArmA and Sul2.【Conclusion】Strains of K. pneumoniae isolated from the drinking water of the chicken farm show serious drug resistance and carried multiple drug resistance genes, among which Mcr-1 gene is detected on the isolated strains. However, the isolated strains are sensitive to polymyxin, and the D-type carbapenemase OXA-48 gene was not detected in the isolated strains.]]></description>
<pubDate>2024/3/21 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[FU Dejia1,2, KANG Huahua2, ZENG Xianjun3,4, FAN Zhihong3,4, CHEN Jie3,4, HUANG Weiqian5, JIANG Shunjin5, PENG Xinyu2]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>FU Dejia1,2, KANG Huahua2, ZENG Xianjun3,4, FAN Zhihong3,4, CHEN Jie3,4, HUANG Weiqian5, JIANG Shunjin5, PENG Xinyu2</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202402012&flag=1]]></guid><cfi:id>4</cfi:id><cfi:read>true</cfi:read></item>
<item>
<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Inspiration of Sex Regulation in Teleosts on Sex Reversal of Zig-zag Eel (Mastacembelus armatus)]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202402013&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[Influenced by two mechanisms of genetic sex determination and environmental sex determination, the sex control of fish is of great significance for aquaculture. Compared with other vertebrates, fishes exhibit great plasticity in sex differentiation and gonadal development. There are significant differences in the size and growth rate of male and female teleosts. Cultivating and breeding large and fast-growing unisexual populations is beneficial to improve economic benefits. Mastacembelus armatus is a kind of freshwater aquaculture fish with high nutritional and economic values. Male individuals grow faster and have a larger body size than females, therefore, cultivating all-male M. armatus has higher economic benefits. In the wild environment, the male to female ratio of the M. armatus is approximately 1∶1, however, there is a high female rate under artificial breeding conditions. At present, it has not been clear what factors cause the feminization of M. armatus, and there have been no successful reports of inducing physiological female sex reversal in the M. armatus, which greatly restricts the development of the M. armatus breeding industry and the improvement of economic benefits. At present, researches on the M. armatus mainly focus on morphology, physiology, reproductive biology, genetic diversity, and other aspects. The research on sex determination and differentiation of the M. armatus is relatively weak, and it is necessary to explore ideas from the sex determination mechanisms, sex regulation methods and sex control breeding techniques of other teleosts. Based on the mechanism of sex determination in teleosts, and the review summarizes several ways of sex control in teleosts, mainly including genetic factors regulation like sex determining genes on sex chromosomes and autosomes, as well as environmental factors regulation, such as temperature, exogenous hormones and fish feed. It aims to provide ideas for analyzing the causes and potential mechanism forhigh feminization of artificial cultivation of the M. armatus, and to provide important theoretical basis for the reproduction and maintenance of unisexual populations, the selection of excellent varieties, and the protection of germplasm resources of the M. armatus, to better understand the gonadal development and reproductive mechanism of the M. armatus, solve the bottleneck of low male rate in currently cultivated M. armatus and provide guarantees for the sustainable development of the M. armatus aquaculture industry.]]></description>
<pubDate>2024/3/21 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[XUE Xiaowen, LU Baoyue, HAN Chong, SHU Hu]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>XUE Xiaowen, LU Baoyue, HAN Chong, SHU Hu</atom:name>
</atom:author>
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<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Application of Autoencoder Interpolation to Enhance the Accuracy of Single-Cell Transcriptomic Analysis in Early Porcine Embryos]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202401010&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】Autoencoder, as one of the deep learning algorithms, offers unique advantages in reducing dimensionality and conducting interpolation analysis on single-cell transcriptome data. The study aims to assess the feasibility of applying the autoencoder AutoClass to early porcine embryo single-cell transcriptome data, and to investigate the impacts of different embryonic activation methods on key genes and signaling pathways.【Method】Single-cell transcriptome data were collected from early porcine embryos with 3 different activation modes (in vivo fertilization, in vitro fertilization, and orphaned females), and AutoClass was used to perform data quality control and interpolation analyses and evaluate AutoClass performance in conjunction with downstream analyses. In addition, an in-depth comparison of key genes and signaling pathways in the three types of embryos was performed by differential expression genes (DEGs) and functional enrichment analysis.【Result】After quality control and autoencoder data interpolation, the accuracy of clustering analysis was enhanced, leading to clear clustering among early embryos with different activation methods. QC alone only screened out 1 287 DEGs, while the number of DEGs increased to 11 523 after autocoder interpolation. Functional enrichment analysis further unearthed key biological processes and signaling pathways that were significantly different among the three different types of embryos, such as basic biological processes and cellular metabolism in porcine in vivo fertilized embryos, gonadal development and sex determination in porcine in vitro fertilized embryos and immune defenses and regulation of secretory pathways in orphaned embryos.【Conclusion】The autoencoder was adopted to improve the analytical accuracy of single-cell transcriptome data, whereby the key genes and signaling pathways affecting early embryonic development in three embryonic activation modes were revealed, which provided new ideas for further in-depth study of the molecular mechanisms underlying early embryonic development in porcine.]]></description>
<pubDate>2024/3/8 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[HUANG Zehang, DU Zhiqiang]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>HUANG Zehang, DU Zhiqiang</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202401010&flag=1]]></guid><cfi:id>2</cfi:id><cfi:read>true</cfi:read></item>
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<title xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="text"><![CDATA[Effects of Partial Replacement of Basal Diets by Fermented Kitchen Waste Feed on Growth Performance and Meat Flavor of Broilers]]></title>
<link><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202401011&flag=1]]></link>
<description xmlns:cf="http://www.microsoft.com/schemas/rss/core/2005" cf:type="html"><![CDATA[【Objective】The study was carried out to evaluate the feasibility and efficacy of feeds produced by fermented kitchen waste to spotted-brown chickens as a partial replacement of basal diets, with a view to providing basic data for the resource utilization and promotion of kitchen waste feed.【Method】One-day-old spotted-brown chickens were selected, and 0% (CK), 10% and 20% fermented kitchen waste feeds (FKWF) were added to the basal diet, and the amount of basal diet was reduced accordingly. At the 63rd day of the test period, the growth performance, slaughter performance and nutritional performance indexes such as crude protein, crude fat, calcium, phosphorus, zinc, selenium, inosinic acid and amino acids in chicken meat were measured.【Result】The results of broiler growth performance showed that the differences in feed-to-weight ratios between broilers in the 10% and 20% FKWF groups and CK were not significant, however, broilers in the 10% FKWF group had the highest weight. The results of slaughter performance showed that there were no abnormality in the skin color, muscle tissue elasticity, pH and odor of broilers of 10% and 20% FKWF groups, and the differences in organ coefficients were not significant compared with that of CK; the results of muscle nutritional performance showed that the crude protein contents of chicken meat in 10% and 20% FKWF groups increased by 7.50% and 3.42%, phosphorus contents increased significantly by 11.44% and 4.37%, and inosinic acid contents increased significantly by 7.10% and 6.00% ; the fresh-flavored amino acid (FAA), sweet amino acid (SAA), essential amino acid (EAA), and total amino acid (TAA) were significantly higher in 10% and 20% FKWF groups than those in CK; aspartic acid, glycine, alanine, valine, isoleucine, leucine, tyrosine, phenylalanine, histidine and cysteine were significantly increased by 4.49%to 12.79% compared with CK ; aspartic acid, valine, isoleucine,and leucine in the 20% FKWF group were significantly increased by 3.37% and 5.74%, compared with CK . 【Conclusion】It is feasible and effective to replace the basal diet by a certain proportion of biofeed produced by fermented kitchen waste to feed broilers, which can save feed costs and improve economic benefits.]]></description>
<pubDate>2024/3/8 0:00:00</pubDate>
<category><![CDATA[Animal Husbandry & Veterinary · Aquaculture]]></category>
<author><![CDATA[ZHANG Pian1, LI Heyue2, YAN Xin3, SHENG Yanming2, LI Jinqing2, GUO Suyin4, WANG Xiaohu1, WANG Gang1]]></author>
<atom:author xmlns:atom="http://www.w3.org/2005/Atom">
<atom:name>ZHANG Pian1, LI Heyue2, YAN Xin3, SHENG Yanming2, LI Jinqing2, GUO Suyin4, WANG Xiaohu1, WANG Gang1</atom:name>
</atom:author>
<guid><![CDATA[http://gdnykx.cnjournals.org/gdnykxen/ch/reader/view_abstract.aspx?file_no=202401011&flag=1]]></guid><cfi:id>1</cfi:id><cfi:read>true</cfi:read></item>
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