| 林宝英，谭志琼，张容意.枯草芽孢杆菌B25抗菌物质初步研究[J].广东农业科学,2013,40(1):82-84 |
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枯草芽孢杆菌B25抗菌物质初步研究 |
| Preliminary study of antimicrobial substance produced by Bacillus subtilis B25 |
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| DOI： |
| 中文关键词: 枯草芽孢杆菌B25 抗菌物质 温度 pH值 蛋白酶 |
| 英文关键词: Bacillus subtilis B25 antimicrobial substance tempertaure pH proteinases |
| 基金项目:国家科技支撑计划项目（2007BAD48B04）；海南大学热带作物种质资源保护与开发利用教育部重点实验室开放基金(2011hckled-05)；海南大学“211工程”建设项目；海南大学环境与植物保护学院研究生创新平台 |
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| 中文摘要: |
| 枯草芽孢杆菌B25能够产生抗菌活性物质，对香蕉枯萎病具较好的抑菌效果。研究了抗菌活性物质产生的时间，以及对温度、pH值和蛋白酶（胰蛋白酶、蛋白酶K）的敏感性；并用10、30、50 ku的超滤管超滤。研究结果表明：抗菌物质是从对数期后期就开始产生的，稳定期活性上升到最大值并一直保持在这个水平；该活性物质对热稳定，可于100℃煮沸1 h而不失活；在酸性、中性及微碱性条件下相对稳定，在强碱性条件下不稳定；对蛋白酶K 和胰蛋白酶敏感；10、30 ku的超滤管时超滤膜下面的滤过液无活性，50 ku超滤管时超滤膜下面的滤过液才有活性。 |
| 英文摘要: |
| Bacillus subtilis B25 is found to produce antimicrobial substance, which has exhibited significant inhibitory effect on Fusarium oxysporum. In the paper,the time of the antimicrobial substance produce, the stability of it on temperature, pH, two proteinases (trypsin, proteinaseK) and the activity with 10, 30, 50 ku ultrafiltration tube filtering were studied. The results showed that the antimicrobial substance not changed significantly even in 100益boil 1 hour; It tolerated acidic, neutral and slightly alkaline but was not stable in strong alkalie. With regard to the two proteinases,the activity decreased obviously when deal with trypsin and proteinaseK. It failed to pass 10尧30 ku filter membrane but can pass 50 ku. |
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