| 毕晶磊，朱利泉，孙全，王微娜，向浏欣，何晓红，江怀仲.茎瘤芥PHYA基因与茎瘤芥茎膨大关系的初步研究[J].广东农业科学,2013,40(1):139-142 |
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茎瘤芥PHYA基因与茎瘤芥茎膨大关系的初步研究 |
| Preliminary study on the relationship between PHYA gene of tumourous stem mustard and stem swelling of tumourous stem mustard |
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| DOI： |
| 中文关键词: 茎瘤芥 光敏色素基因 超表达 茎膨大 |
| 英文关键词: tumours stem mustard phytochrome A gene over-expression swell |
| 基金项目:国家自然科学基金（31071461,30771311）；重庆市重点攻关项目（CSTC2011AB1095）；重庆市教委项目（KJ110506）；重庆市自然科学基金（cstc2007BB1328） |
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| 中文摘要: |
| 从茎瘤芥基因组中克隆了全长PHYA 基因，构建了植物超表达载体，通过农杆菌GV3101 转化茎瘤芥——永安小叶，得到PCR反应呈阳性的转基因株系。结果发现，在自然生长环境下，转基因植株发生了多种形态变异，如植株矮化、花期提前、叶片变大且色泽浓绿、转基因植株茎膨大延缓等。初步推断PHYA 基因参与调控茎瘤芥植物茎膨大过程，过量表达PHYA 基因可能会抑制茎膨大过程。 |
| 英文摘要: |
| In this study, we cloned a full-length PHYA cDNA from the total RNA of tumours stem mustard, made a expression
analysis in different organ and swelling development stage, then we constructed a overexpression vector and converted tumourous stem mustard through a Agrobacterium GV3101 by the calli dipping method. Finally, we obtained a transgenic plant that was validated by PCR.Compared with the WT tumourous stem mustard, the transgenic plant showed a conspicuous difference, dwarf stature, an early anthesis,deep green leaves and delayed stem swelling. According to these results, we presumed that PHYA gene may play an important role in the regulation system of stem swelling, and over-expression of PHYA may delay swelling development. |
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