| 李建湘，王振中.5种瓜类植物多聚半乳糖醛酸酶抑制蛋白的诱导表达及活性测定[J].广东农业科学,2013,40(1):143-146 |
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5种瓜类植物多聚半乳糖醛酸酶抑制蛋白的诱导表达及活性测定 |
| Induced expression and activity detection of Polygalacturonaseinhibiting protein in 5 kinds of cucurbits crops |
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| DOI： |
| 中文关键词: 瓜类枯萎病 尖孢镰刀菌 多聚半乳糖醛酸酶 多聚半乳糖醛酸酶抑制蛋白 |
| 英文关键词: fusarium wilt of cucurbits crops Fusarium oxysporum polygalacturonase polygalacturonase-inhibiting protein |
| 基金项目:国家自然科学基金（30971888）；国家公益性行业渊农业冤科研专项（200903049） |
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| 中文摘要: |
| 多聚半乳糖醛酸酶抑制蛋白（PGIP）是一种富含亮氨酸重复单位的糖蛋白，能非竞争性地抑制真菌多聚半乳糖醛酸酶（PG）的活性，从而提高植物的抗病性。以黄瓜、苦瓜、冬瓜、节瓜和甜瓜幼苗为材料，研究PGIP的诱导表达特性及在不同组织中的含量差异。通过比较经孢子诱导、SA诱导和黑暗诱导的黄瓜幼苗中的PGIP的相对含量，发现经病原菌孢子悬浮液处理48 h时PGIP 的表达量最高。对经孢子诱导48 h的黄瓜幼苗的根、茎尧叶中的PGIP含量进行比较，发现茎中PGIP的相对含量最高。测定这5种瓜类的PGIP对6种尖孢镰刀菌粗PG的抑制作用，发现苦瓜PGIP的相对活性较高，并证实了不同的PGIP能够特异性地识别不同的PG，PGIP对不同PG 存在着选择性抑制。 |
| 英文摘要: |
| Polygalacturonase-inhibiting protein (PGIP) is a leucine-rich repeat cell wall-binding protein that can inhibit the activity of fungal PG specifically and non-competitively, thus improve the disease resistance of plants. Cucurbitaceae cucumis, Momordica charantia, Benincasa hispida, Benincasa hispida var. chieh-qua and Cucumis melo seedlings were selected in this research. Biotic stressed plants of C. cucumis showed high induced expression activity, rose up to maximum at 48 h and stems showed maximum expression than other organs. Biotic stressed plants of 5 kinds of cucurbits crops are used to prepare crude inhibitor and their activity was checked by inhibition assay to 6 kinds of PG respectively, PGIP of M. charantia showed high activity than others PGIP. The results showed a wide spectrum of activity of PGIP against different PG and there might be selective inhibition. |
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