| 王庆容，宋培，唐立俊，喻哓丹.不同地理群体的鲇线粒体DNA 16S rRNA基因序列比较分析[J].广东农业科学,2013,40(1):150-153 |
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不同地理群体的鲇线粒体DNA 16S rRNA基因序列比较分析 |
| Comparison and analysis of the five wild Chinese catfish(Silurus asotus Linnaeus) populations based on mitochondrial DNA 16S rRNA sequences |
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| DOI： |
| 中文关键词: 鲇 线粒体DNA 遗传多样性 16S rRNA基因 |
| 英文关键词: Silurus asotus mitochondrial DNA genetic diversity 16S rRNA gene |
| 基金项目:贵州省科技厅项目（黔科合J字2011-2189） |
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| 摘要点击次数: 1896 |
| 全文下载次数: 647 |
| 中文摘要: |
| 对长江中上游主要支流5个野生群体鲇【舞阳河群体（WYH）、乌江群体（WJ）、雅砻江群体（YLJ）、岷江群体（MJ）、金沙江群体（CS）】中的27 个样品的16S rRNA基因进行PCR 扩增，并对扩增产物测序。用CLUSTAL X v 1.81软件对所得的27个16SrRNA 序列进行比对，并用Dna SP 5.10软件进行比较分析，共检测出74个变异位点、8种单倍型。舞阳河、乌江、雅砻江、岷江、金沙江等5个野生群体的单倍型多样性（H）分别为0.600、0.857、0.600、0.600、0.667，核苷酸多样性（仔）分别为0.00072、0.1754、0.00036、0.00072、0.01871。对5个野生群体的16S rRNA 序列进行Tajima’s D和Fst 分析发现，所有群体符合中性进化模型，且有一些单倍型的分化，只有金沙江群体的遗传差异显著。对5个群体进行分子变异等级分析的结果表明，群体间分子变异不显著。对5个群体构建分子系统树发现，乌江、雅砻江、岷江、金沙江群体之间的亲缘关系较近，舞阳河群体与其他群体间的亲缘关系较远。 |
| 英文摘要: |
| The aim of the present study was to survey mtDNA 16S rRNA sequence variation to assess genetic diversity and genetic
structure of the wild Silurus asotus in the upstream and midstream of Yangtze River. The samples of S. asotus have been obtained from the midstream and upstream of Yangtze River included the Wuyanghe (WYH), Wujiang River (WJ), Yalong Jiang (YLJ), Min River (MJ) and Chin-sha River (CS). The PCR technique was used to amplify the mtDNA 16S rRNA among 27 individuals. The PCR products were purified and sequenced. As a result, 1 680 bp nucleotide sequences of 16S rRNA were obtained. 8 haplotypes and 74 variable sites were detected among all mitochondrial 16S rRNA sequences of 27 Chinese catfish. Haplotype diversities (H) in the Wuyanghe putative population, Wujiang River putative population, Yalong Jiang putative population, Min River putative population and Chin-sha River putative population were 0.600, 0.857, 0.600, 0.600 and 0.667 respectively. Nucleotide diversities (仔) in these putative populations were 0.00072, 0.1754, 0.00036, 0.00072 and 0.01871 respectively. Tajima爷s D values showed that all putative populations fited to neutral evolution model, and FST values among all the other populations showed no strong genetic differences expect Chin-sha River (CS).Nosignificant genetic differentiation among all five putative populations were found by analysis of molecular variance (ANOVA). The ML
tree of the five putative populations showed Wujiang River (WJ), Yalong Jiang (YLJ), Min River (MJ) and Chin-sha River (CS) putative populations had the closest relation among the five putative populations, the Wuyanghe (WYH) putative populations had the greatest genetic distance among the five putative populations. |
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