| 刘志典，张秀君.土壤线虫培养方法研究[J].广东农业科学,2013,40(5):60-62 |
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土壤线虫培养方法研究 |
| Study on the culture of soil nematode |
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| DOI： |
| 中文关键词: 土壤线虫 培养 温度 |
| 英文关键词: soil nematode culture temperature |
| 基金项目:国家自然科学基金（40875088） |
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| 摘要点击次数: 1327 |
| 全文下载次数: 670 |
| 中文摘要: |
| 土壤线虫作为模式生物的一种, 已广泛应用于土壤生物群落和土壤污染的指示研究，但有关土壤线虫的培养方法鲜有报道。在实验室条件下，利用3种培养基（生菜蛋黄培养基、葡萄糖酵母菌培养基及土壤浸出液）、5 个温度梯度（15、20、25、30、35℃）对土壤线虫进行培养。结果显示，与土壤培养液和葡萄糖酵母菌培养基相比，土壤线虫更适宜在生菜蛋黄(lettuce eggs，LE)培养基中生长；在15~35℃的温度范围内，最佳的培养温度为25℃。 |
| 英文摘要: |
| Soil nematode, as a kind of model organism, has been widely used in the deictic research on biological communities in soil
and soil pollution, but there are few reports on the cultivation of soil nematodes. This paper studied the cultures of soil nemafode based on the cultivation of the soil nematodes in the laboratory conditions, with three kinds of medium, lettuce eggs (LE) culture medium, glucose yeast culture medium and soil extract, and at five temperature gradient (15, 20, 25, 30 and 35℃). The result showed that lettuce egg culture medium (lettuce eggs, LE) was more suitable than the other two media for the incubation of the soil nematode; and in the 15~35℃ temperature range, the best incubation temperature was 25℃. |
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