| 罗书芳，崔浪军，王健，屈生宪.干旱胁迫下15种丹参miRNAs差异表达分析[J].广东农业科学,2013,40(5):134-137 |
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干旱胁迫下15种丹参miRNAs差异表达分析 |
| Differential expression of 15 miRNAs in Salvia miltiorrhiza Bunge under drought stress |
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| DOI： |
| 中文关键词: 丹参 RT-PCR 干旱胁迫 差异性表达 |
| 英文关键词: Salvia miltiorrhiza Bunge RT-PCR drought stress differential expression |
| 基金项目:国家博士后基金(2008043125)；教育部高等学校博士学科点专项(200807181002)；陕西省教育厅自然科学专项基金（11JK0610）；安康学院高层次人才专项基金（AYQDZR200926） |
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| 中文摘要: |
| 参考已报道的其他植物中响应干旱胁迫的miRNAs，以其中15 个miRNAs为研究对象，10% PEG6000 胁迫处理的丹参幼苗为材料，采用实时定量PCR 技术分析了这些miRNAs在干旱胁迫前后丹参幼苗叶片中的差异性表达。结果表明，在干旱胁迫6 h后, 丹参体内miR156、miR157、miR166、miR168、miR169、miR319、miR774、miR894、miR2911、miR3462 以及miR3626 的表达水平表现为不同程度的上调，其中miR157、miR166、miR319、miR774、miR894、miR2911和miR3626 表现为显著性上调；miR159、miR167、miR172以及miR408 则表现为下调。对这些miRNAs作用的靶基因进行预测和分析发现，它们主要参与植物的新陈代谢、信号传导和生长发育过程，推测miR169 和miR3462参与了丹参对干旱胁迫应答过程。 |
| 英文摘要: |
| 15 candidate miRNAs in Salvia miltiorrhiza seedlings were studied after the herbs treated with 10% PEG6000 for 6 h.The results showed that the expression levels of the 11 miRNAs in drought treated seedlings were up-regulated in comparison with the control seedlings, in which miR157, miR166, miR319, miR774, miR894, miR2911 and miR3626 were up -regulated significantly.However, the expression levels of miR159, miR167, miR172 and miR408 were down-regulated. The analysis on the target genes of these miRNAs revealed that these miRNAs primarily participated in the plant metabolism , signal transduction and growing development, and inferred that miR169 and miR3462 had taken part in the process of drought stress. |
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