| 马勇，陈秀莉，张应龙，门中华.一株产油酵母菌的微波诱变选育[J].广东农业科学,2013,40(6):118-119 |
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一株产油酵母菌的微波诱变选育 |
| Breeding of a strain of oleaginous yeast via the microwave mutation |
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| DOI： |
| 中文关键词: 产油酵母 微波诱变 诱变选育 油脂产量 |
| 英文关键词: oleaginous yeast microwave mutation mutation breeding oil production |
| 基金项目:国家自然科学基金(31160254)；校内青年科学研究基金(BSYKJ2011-17) |
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| 中文摘要: |
| 以产油酵母菌Y1为试验菌株，通过对出发菌株Y1的最佳微波诱变时间确定、诱变后菌株初筛和复筛后得到一株突变菌株Y13，测得Y13 的油脂产量4.39 g/L、油脂含量22.09%，比出发菌株Y1油脂产量2.11 g/L、含油量9.6%明显提高。因此，菌株Y13 可作为后续摇瓶发酵产脂研究的试验菌株。 |
| 英文摘要: |
| The mutant strain Y13 was isolated by the time of optimum microwave mutagenesis, mutagenic strains of screening and
re-screening within oleaginous yeast Y1. The results showed that Y13 was significantly improved in oil production and content, the oil production reached 4.39 g/L from 2.11 g/L and the content reached 22.09% from 9.6%, respectively. It is feasible to employ Y13 as original strains using shake-flask fermentation for lipid-producing. |
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